Papers by Mojgan Shaiegan
Scientific Journal of Iran Blood Transfus Organ, Jul 10, 2021
Scientific Journal of Iran Blood Transfus Organ, Oct 10, 2021
Scientific Journal of Iran Blood Transfus Organ, 2016
Knowing the human platelet angens (HPA) and genes frequency in different populaons is important n... more Knowing the human platelet angens (HPA) and genes frequency in different populaons is important not only for populaon studies but also for clinical transfusion pracce. HPA genes frequency in Iran is not evaluated, so we invesgated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 paents with platelet refractoriness) was isolated from peripheral blood mononuclear leucocytes and tested by restricted fragment length polymorphism analysis (RFLP). Results: The frequency of HPA phenotypes was determined as follows: (in donors) HPA-2a/2a:75.4%, HPA- 2a/2b:24.8%, HPA-3a/3a:8.1%, HPA-3a/3b:70.7%, HPA-3b/3b:21.2%, HPA-5a/5a:99 % and HPA-5a/5b:1%; (in paents) HPA-2a/2a:66.7%, HPA-2a/2b:33.3%, HPA-3a/3a:23.8%, HPA-3a/3b: 47.6%, HPA-3b/3b:28.6%, HPA-5a/5a:90.5%, and HPA-5a/5b: 9.5%. Frequency of genotypes evaluated by RFLP were as below: HPA-2a=0.87, HPA-2b=0.13, HPA- 3a =0.28, HPA-3b=0.72, HPA-5a=0.99, and ...
International Journal of Hematology-Oncology and Stem Cell Research, 2022
Background: An analysis of red blood cell alloimmunization in patients with thalassemia can help ... more Background: An analysis of red blood cell alloimmunization in patients with thalassemia can help to devise specific strategies to decrease the alloimmunization rate. This study explored the frequency and specificity of alloantibodies and autoantibodies against red blood cell (RBC) antigens in patients with thalassemia referring to the Iranian Blood Transfusion Organization (IBTO) Immunohematology Reference Laboratory (IRL) in Tehran. Materials and Methods: This study first examined the laboratory records of 23,113 patients suffering from different diseases referring to IBTO’s IRL for pretransfusion testing in the 2008-2015 period. ABO and Rh(D) typing and antibody screening tests were performed for all 23,113 patient records and 685 (2.97%) beta-thalassemia patients with positive pre-transfusion test results (antibody screening and/or DAT) were selected for further investigation. Results: The antibody screening test was positive in 640 out of 685 thalassemic patients (93.4%). DAT wa...
Iranian Journal of Basic Medical Sciences, 2015
Human cytomegalovirus (CMV), a double-strand DNA herpesvirus, can be transmitted via blood transf... more Human cytomegalovirus (CMV), a double-strand DNA herpesvirus, can be transmitted via blood transfusion which is especially important for immunocompromised recipients and can cause a fatal infection. CMV seroprevalence in Iran was studied on blood donors, healthy subjects, and some patients. Highly variable rates were detected. The purpose of this study was to review CMV seroprevalence in blood donors and apparently healthy individuals, in Iran. One hundred and fifty-eight electronic and paper-based resources and databases including published articles in internal and external journals, seminars, dissertations, and theses available in the database and different websites were used to be systematically reviewed as a meta-analysis. Less related articles to the issue, papers of specific high risk population, and articles with not enough information, were excluded. Eventually 22 articles that satisfied our selection criteria were systematically reviewed and analyzed. To explore heterogenei...
The aim of this study was to evaluate mixed red cells population and red blood cell chimerism aft... more The aim of this study was to evaluate mixed red cells population and red blood cell chimerism after hematopoietic stem cell transplantation. Red blood cell chimerism after hematopoietic stem cell transplantation was analyzed using a series of fluorescein isothiocyanate-conjugated monoclonal antibodies (BioAtlantic, France) directed against ABH, Rh (D, C, E, c, e), Kell, Duffy, Kidd, and Ss antigens on blood samples of 14 patients with hematologic disorders undergoing hematopoietic stem cell transplantation, by flow cytometric method on days 15, 30, and 60 after transplantation. All patients showed expression of donor red cell antigens within days 15-30 after hematopoietic stem cell transplantation. Graft versus host disease and ABO incompatibility did not affect the expression of chimerism. Flow cytometric analysis is a simple, accurate, and valuable test which is of significant help in monitoring chimerism in allogeneic hematopoietic stem cell transplantation.
Reports of biochemistry & molecular biology, 2016
BACKGROUND Interfering with cell proliferation and survival is a critical role for antineoplastic... more BACKGROUND Interfering with cell proliferation and survival is a critical role for antineoplastic drugs leading to cell death through induction of apoptosis. Alternative treatments with herbal extracts offer insights into acute myeloid leukemia (AML) therapy. Parthenolide (PTL), an extract from feverfew, induces apoptosis in primary human leukemia stem cells (LSCs) and bulk leukemic cell populations. Osteopontin (OPN) preserves cell viability in response to anticancer agents and its receptors could be utilized for therapeutic targeting of cancer cells. METHODS U937 cells were cultured in RPMI 1640 with concentrations of 2, 4, 6, 8, and 10 µM PTL for 20-24 hours for MTT assays. Apoptosis assays were performed with Annexin V-Alexa Fluor-488/PI as Annexin V+/PI- and Annexin V+/PI+ to measure early and late apoptosis, respectively. Quantitative real-time PCR was used to measure OPN gene expression using the 2(-ΔΔCt) method. The PTL-treated cells were stained with FITC-CD38 antibody for ...
International Journal of Hematology-Oncology and Stem Cell Research, 2016
Background: Acute myeloid leukemia (AML) is an immunophenotypically heterogeneous malignant disea... more Background: Acute myeloid leukemia (AML) is an immunophenotypically heterogeneous malignant disease, in which CD34 positivity is associated with poor prognosis. Osteopontin (OPN) plays different roles in physiologic and pathologic conditions like: survival, metastasis and cell protection from cytotoxic and apoptotic stimuli. Due to anti-apoptotic effect of OPN in normal and malignant cells, silencing of OPN leads to elevation of sensitivity towards chemotherapeutic agents and attenuates cancer cells migration and invasion. Therefore, the aim of this study was to evaluate OPN roles in modulating curcumin-mediated growth inhibitory on leukemic stem cells (LSCs) colony forming potential and survival in AML cell lines and primary CD34+/CD38- bone marrow-derived AML cells. Materials and Methods: Primary human CD34+/CD38- cells were isolated from bone marrow mononuclear cells of 10 AML patients at initial state of diagnosis, using a CD34 Multi sort kit. The growth inhibitory effects of cu...
Iranian journal of pediatric hematology and oncology, 2020
Background: Human platelet antigens (HPAs) are part of platelet GP complexes have the potential t... more Background: Human platelet antigens (HPAs) are part of platelet GP complexes have the potential to contribute to the autoantibody production. Moreover, these antigens demonstrate different patterns of distribution on different ethnic groups and variation in some types of diseases. This study was objected to determine the incidence of HPA-1 to -5 and -15 polymorphisms in the Iranians suffering from primary Immune thrombocytopenic purpura (ITP). Materials and Methods: In this case-control investigation, 30 patients by definite primary ITP were randomly selected and enrolled in the study. HPA genotyping was performed implicating by the Single Specific Primer PCR (SSP-PCR). For the control group, data of recently published gene polymorphism among Iranian Blood donors were deployed for comparison. Results: The incidence of HPA-1 to -5 and -15 polymorphisms in the Iranian patients with primary ITP was found to be: HPA-1a/1a: 0.933, HPA-1a/1b: 0.067, HPA-2a/2a: 0.133, HPA-2a/2b: 0.867, HPA...
Scientific Journal of Iran Blood Transfus Organ, 2017
Scientific Journal of Iran Blood Transfus Organ, 2018
Background and Objectives Estimation of the frequency and causes of immunization to red blood cel... more Background and Objectives Estimation of the frequency and causes of immunization to red blood cells in transfusion dependent thalassemia (TDT) patients can help us know the limitations of current practice and plan specific measures to reduce it. This study aimed to evaluate the frequency and causes of immunization in TDT patients. Materials and Methods This case control study was conducted on 732 (TDT) patients between 20 November 2015 to November 2016 in Dastgheib Hospital, Shiraz, Iran. Direct coombs test, antibody screening, and antibody identification were performed for every TDT patient. Then, the frequency and type of antibodies were surveyed. Clinical and laboratory data of immunized and nonimmunized patients were compared. Results All of the case group had positive results in direct or indirect coombs tests. Indirect coombs test (ICT) was positive in 59 patients (63.4%) while direct coombs tests (DCT) was positive in 41 (44%) of immunized patients. In addition, 7 patients (7...
Therapeutic Apheresis and Dialysis
BACKGROUND This study was designed to evaluate platelet transfusion outcome via flow cytometric m... more BACKGROUND This study was designed to evaluate platelet transfusion outcome via flow cytometric monocyte phagocytic assay (FMPA). METHODS Fifteen patients with a history of multiple platelet transfusions and fifteen controls were enrolled in this study. CMFDA-labeled platelets were incubated with patients' sera and were finally incubated with monocytes in a tube and analyzed by flow cytometry. Monocytes that phagocytosed platelets were detected as a CMFDA-positive platelet population via monocyte gate. The FMPA results were compared with CCI results for the patients. RESULTS The FMPA result correlated with 1-hour (r = -0.885, P = 0.001) and 24-hour (r = -0.884, P = 0.001) CCI. There is a significant difference in means of FMPA results between the patients with immune platelet refractoriness (68.46 ± 10.4%), non-refractory group (37.73 ± 15.21%) and the control group (18.27 ± 2.86%). CONCLUSION Our data showed that FMPA has good results in evaluation of platelet transfusion ou...
Archives of Iranian medicine, 2016
Three main goals were considered in this celebration: 1To honor and appreciate all hematopoietic ... more Three main goals were considered in this celebration: 1To honor and appreciate all hematopoietic stem cell donors (including related donors, unrelated donors, parents who donate their baby’s cord blood, and volunteers who are waiting to donate their stem cells) 2To promote the culture of stem cell donation in public society. 3-To encourage voluntary citizens or non-governmental organizations to help, fund and support stem cell donation and registries in the country. A number of stem cell donors (Adult and cord blood donors) in recent years in Iran were appreciated during the ceremony. The BMDW (Bone Marrow Donor Worldwide) database contains 28,796,748 donors and 705,134 cord blood units, according to the reports on BMDW website in November 2016. Currently different registries are established for recruitment
Iranian Journal of Allergy, Asthma and Immunology
Human platelet antigens (HPAs) are glycoproteins on the platelet surface that a single nucleotide... more Human platelet antigens (HPAs) are glycoproteins on the platelet surface that a single nucleotide mutation in the coding region gene could lead to the variation of different HPA polymorphisms. These antigens have shown variation among different races and may trigger immune responses during blood transfusion and pregnancy. Genotyping of HPAs is useful for managing these reactions and establishing a platelet registry to decrease platelet transfusion reactions. This study aimed to compare allelic and genotype frequencies of human platelet antigens in the Azeri ethnicity by TaqMan Real-time and polymerase chain reaction with sequence-specific primers (PCR-SSP) methods. DNA was extracted from the whole blood of 100 Azeri blood donors in the Ardabil Blood Transfusion Center. Genotyping of HPA-1 to -5 and -15 was performed by TaqMan Real-time PCR, and PCR-SSP and consistency of results were evaluated. The results of PCR-SSP and TaqMan Real-time PCR showed complete consistency. The allele f...
International Journal of Cancer Management, Apr 28, 2018
Background: Daunorubicin (DNR) is capable of killing the human acute myeloid leukemia cells throu... more Background: Daunorubicin (DNR) is capable of killing the human acute myeloid leukemia cells through apoptosis or necrosis with arresting cell cycle and various mechanisms. The response of AML cells to DNR associated with defect and or defiance in survival has been a consideration subject. Objectives: We have represented the transcription gene profile of some critical prosurvival proteins by qRT-PCR, including osteopontin (OPN), AKT1, mTOR, β-catenin, and NF-kB/RelB. Methods: The U937 cells were treated with DNR with clinically achievable concentrations for MTT assay, annexin V (AV) /Propidium iodide (PI), and cell cycle analysis. QRT-PCR was performed, using primers of OPN, NF-kB/RelB, AKT1, mTOR, PTEN, and β-catenin. Results: The AV/PI assay displayed that DNR-induced death in cells was a dose-dependent and necrotic manner. Cell cycle distribution following treatment with DNR exhibited a relatively lower chromatin of S phase than untreated cells. OPN gene expression was significantly attenuated. NF-kB/RelB, mTOR, β-catenin, as well as PTENgenes showed unchanged or non-significant increase in expression. However, AKT1increased significantly. Conclusions: U937 sensitivity to DNR could be due to the targeting of anti-apoptotic proteins in the transcriptional stages. The decrease in OPNlevels appears to play a significant role in the death of the observed by DNR.
Quality of life has emerged as an important concept and outcome in health and health care. This s... more Quality of life has emerged as an important concept and outcome in health and health care. This study was performed to evaluate the ability of the health education program to improve quality of life of patients with coronary artery bypass graft surgery (CABG). In pre operation period, 70 patients were randomized in two groups, experimental and control group (35 patients in each). The demographic information, SF-36 and Nottingham Health Profile questionnaire were administered before surgery to all patients. Patients in the experimental group received the educational intervention according to Mico ' s education planning model. These patients were followed up to 1-month. At result, SF-36, Nottingham Health Profile questionnaires were administered 1-month after education by patients again. Significant improvements in quality of life between two groups, as measured by the Nottingham Health Profile, were seen in energy (P < 0.001), pain (P < 0.006), emotional reaction (P < 0....
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Papers by Mojgan Shaiegan