Papers by Mohammad Zibaei
International Journal of Biomedical Engineering and Technology, 2023
Iranian Journal of Health and Environment, Aug 10, 2020
Optics and Photonics Society of Iran, Mar 10, 2019
Optics and Photonics Society of Iran, Mar 10, 2019
In this paper, an efficient method is proposed for the temperature compensation of optical voltag... more In this paper, an efficient method is proposed for the temperature compensation of optical voltage sensors. In this method, the x-cut LiNbO3 bulk crystal with optical path parallel to z-axis with two orthogonal output signals is used. By using the two output signals and the compensation algorithm, the effect of the temperature and laser intensity was eliminated, and the electric field intensity parameter was measured with high accuracy and the mean error reduced by %1.3. This method has been successfully implemented in order to solve the temperature challenge in optical transformer, designed and constructed by the Niroo Research Institute (NRI), were successfully implemented after passing the industrial tests and ensuring their reliability.
International Journal of Enteric Pathogens, May 23, 2018
Journal of Bacteriology & Parasitology, Aug 31, 2015
Iranian Journal of Parasitology, Dec 15, 2020
Background: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by ... more Background: Toxocariasis is one of the most neglected zoonotic diseases, predominantly caused by Toxocara canis. We aimed to evaluate the expression of microRNAs 21 and 103a in seropositive individuals for human toxocariasis as diagnostic biomarkers. Methods: This study was conducted on 324 individuals for ELISA test on toxocariasis in Tehran and Karaj, Iran 2019. Then positive samples for anti-Toxocara IgG were obtained to quantitative Real-time PCR (qRT-PCR) assays to investigate the transcriptional profiles of miRNAs predicted to be involved in developmental and reproductive processes. qPCR was employed to assess levels of transcription for miRNAs of 103a and 21 in plasma samples. Results: After the experiments, the results were evaluated by REST software, Livak formula and quantitative t-test. The analyzes performed on human samples showed that in the case group compared to the control group, only in Tc-miR-21 gene, a 0.3fold increase in expression was obtained with REST software (Fold change ≤ 1.5, P>0.05), which was statistically significant by t-test (P<0.05). Conclusion: To our knowledge, this is the first study to evaluate miR-21 and miR-103a in toxocariasis, which shed light on the fundamental role of it as a biomarker and diagnostic tool. However, due to the changes in expression of these miRNAs were not vast to be used as biomarkers in diagnosis. Despite of that the changes in the expression of these miRNAs were not vast but they could serve as novel promising biomarkers for diagnosis of toxocariasis.
Antimicrobial Resistance and Infection Control, Jul 17, 2020
Background: The global spread of methicillin-resistant Staphylococcus aureus (MRSA) infections ne... more Background: The global spread of methicillin-resistant Staphylococcus aureus (MRSA) infections necessitates the use of validated methods for the identification and typing of this bacterium. This study aimed to determine the distribution of main molecular types of MRSA strain circulating among hospitalized patients in teaching hospitals in Isfahan and Kashan. Methods: A total of 146 Staphylococcus aureus strains were isolated from patients in four teaching hospitals in Isfahan and Kashan during June 2017 to September 2018. The antimicrobial resistance patterns of Staphylococcus aureus strains were performed by disc diffusion method. The MRSA strains were identified phenotypically and confirmed by PCR assay. The prevalence of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) genes among MRSA strains was evaluated by multiplex PCR. The genotypes of MRSA strains were determined by multilocus sequence typing and SCCmec typing. Results: Of 146 Staphylococcus aureus isolates, 24 (16.4%) isolates were identified as MRSA strains. According to antimicrobial susceptibility testing the highest resistance rates were seen for tetracycline, erythromycin, ciprofloxacin and gentamicin. All of Staphylococcus aureus isolates were susceptible to vancomycin whereas 3 (2.1%) isolates were resistant to linezolid. Three different SCCmec types were obtained among MRSA strains including 16 (66.7%) SCCmec type V, 3 (12.5%) SCCmec type III and 5 (20.8%) SCCmec type II. Of 24 MRSA isolates 20 (83.3%) carried MSCRAMMs genes including eno (70.8%), fib (54.1%), cna (25.0%), fnbB (16.6%), ebps 5 (20.8%), and the fnbA, bbp and clfA genes were not detected in any MRSA isolate. MLST analysis revealed 11 sequence types among MRSA isolates as follows:
Pain Physician, Aug 14, 2012
Background: Headache is a prevalent and disabling condition affecting people in all age groups wo... more Background: Headache is a prevalent and disabling condition affecting people in all age groups worldwide, leading to low job performance and quality of life with a significant economic burden on societies. Objective: We evaluated headache prevalence and characteristics and some probable associated factors in patients referring to neurology specialist clinics.
Jundishapur Journal of Microbiology, Feb 7, 2015
Background: Urinary tract infection (UTI) is one of the most prevalent infectious diseases. Uropa... more Background: Urinary tract infection (UTI) is one of the most prevalent infectious diseases. Uropathogenic Escherichia coli (UPEC), as the most important cause of UTI, are associated with a number of virulence factors. Objectives: The current study aimed to investigate the virulence associated determinants as well as their patterns of antibiotic resistance in UPEC isolated from hospitalized patients with UTI. Materials and Methods: A total of 150 E. coli isolates were collected from patients with UTI from December 2012 to June 2013 in Kashan, Iran. Antimicrobial susceptibility screening of 12 antibiotics was determined using disk diffusion method. Polymerase Chain Reaction (PCR) assay was used to detect virulence-related genes in UPEC strains. The purified PCR products were sequenced. Results: Of the total 150 UPEC isolates, 111 (74%) were multidrug-resistant. High resistance was observed against ampicillin (81.3%), nalidixic acid (71.3%), cotrimoxazole (64.7%) and ciprofloxacin (61.3%), respectively. Eighty-four out of the 150 isolates showed resistance against the extended spectrum cephalosporins. Totally, virulence genes were detected in 126 (84%) UPEC isolates .The PCR results identified the traT gene in (74%), PAIs markers in (61.3%) and the pap gene in (16.6%) of the isolates. Conclusions: The traT gene and PAI markers were highly prevalent among UPEC strains isolated from patients in Kashan, Iran; therefore these determinants could be used as targets for prophylactic interventions. Also there was a high level of resistance against the antibiotics commonly used for urinary tract infection treatment. To reach better therapeutic outcomes, treatment regimens have to be modified.
نشریه دانشگاه علوم پزشکی البرز, Aug 1, 2022
Frontiers in Pharmacology, Sep 20, 2022
Background: Recently, a hypothesis about the negative relationship between cancers and parasites ... more Background: Recently, a hypothesis about the negative relationship between cancers and parasites has been proposed and investigated; some parasitic worms and their products can affect the cancer cell proliferation. Due to the potential anti-cancer effect of helminthic parasites, in the present study, the excretory-secretory protein of Toxocara canis (T. canis) parasite was used to evaluate the possible anti-cancer properties and their effect on gastrointestinal and liver cancer cell proliferation-related genes in laboratory conditions. Methods and materials: The selected synthesized peptide fraction from the T. canis excretory-secretory Troponin protein peptide (ES TPP) was exposed at 32, 64, 128, and 256 μg/ml concentrations to three gastrointestinal cancer cell lines AGS, HT-29, and Caco 2, as well as HDF cells as normal cell lines. We used the MTT assay to evaluate cellular changes and cell viability (CV). Variations in gene (Bcl-2, APAF1, ZEB1, VEGF, cyclin-D1, and caspase-3) expression were analyzed by real-time RT-PCR. Results: After 24 h of exposure to pept1ides and cell lines, a decrease in CV was observed at a concentration of 64 μg/ml and compared to the control group. Then, after 48 h, a significant decrease in the CV of Caco 2 cells was observed at a concentration of 32 μg/ml; in the other cancer cell lines, concentrations above 32 μg/ml were effective. The peptide was able to significantly alter the expression of the studied genes at a concentration of 100 μg/ml. Conclusion: Although the studied peptide at high concentrations could have a statistically significant effect on cancer cells, it is still far from the standard drug RECEIVED
Polymer Bulletin, Apr 2, 2022
The initial step in molecular biology and genetic engineering fields are the finding of optimal m... more The initial step in molecular biology and genetic engineering fields are the finding of optimal methods for the extraction of the genomic DNA from microorganisms and cells. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is a novel method for the isolation of nucleic acid. In the present study, the synthesized magnetic nanoparticles were used to extract the genomic DNA of Escherichia coli. In this study, the naked (Fe3O4) and coated (Fe3O4/SiO2; Fe3O4/SiO2/TiO2) magnetic nanoparticles were synthesized by precipitation methods. The purity of the product was assessed using energy-dispersive x-ray spectroscopy (EDS) and x-ray crystallography. The morphology and size of specimens were observed via field emission scanning electron microscopy (FESEM). The efficacy of the synthesized nanoparticles in genomic DNA extraction from Escherichia coli strain ATCC25922 was compared. The quality of the resulting DNA was analyzed by NanoDrop spectrophotometer, agarose gel electrophoresis, and PCR assays. According to the findings of SEM, the size of Fe3O4/SiO2/TiO2 magnetic nanoparticles was about 43.25 nm (Fe3O4/SiO2/TiO2). XRD analysis showed that the product nanoparticles were synthesized without impurities. The extraction results also showed that the purity and concentration of extracted genomic DNA by coated magnetic nanoparticles in comparison with naked magnetic nanoparticles were higher. In addition, the use of 0.4 mg of nanoparticles and 80% polyethylene glycol were optimal conditions for achieving a higher quality of DNA. According to the obtained results, the coated magnetic nanoparticles Fe3O4/SiO2/TiO2 can be used to extract genomic DNA of high quality from gram-negative bacteria.
International Journal of Enteric Pathogens, Dec 16, 2017
It is vital that this meeting be attended by scientists and scholars working in the field of medi... more It is vital that this meeting be attended by scientists and scholars working in the field of medicine as well as by clinicians, epidemiologists, and social scientists concerned with preventive and other aspects of medical research. The reasons for this are simple. The National Clinical Case Reports is the most media-intensive event in the world in regard to dissemination of information about the diseases. The program included an opening ceremony, 1 invited lecture, 1 closing lecture, 5 symposia on selected topics,
Annals of Clinical Microbiology and Antimicrobials, May 6, 2023
Background Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global health crisis. This st... more Background Carbapenem-resistant Acinetobacter baumannii (CRAB) is a global health crisis. This study aimed to determine the clonal relatedness of antibiotic-resistant A. baumannii isolates in hospitalized patients who suffered from burn wound infection. Methods One hundred and six A. baumannii isolates from 562 patients with burn wound infections, were identified and examined for antimicrobial susceptibility. Detection and characterization of carbapenem-hydrolyzing class D OXA-type beta-lactamases (CHDLs) were performed by PCR assays. The clonal relatedness of A. baumannii isolates was determined by multilocus sequence typing (MLST) according to the Pasteur scheme, dual-sequence typing of bla OXA−51-like and ampC genes, and RAPD-PCR method. Results All isolates were carbapenem-resistant while susceptible to colistin, minocycline, doxycycline, and ampicillinsulbactam. The intrinsic bla OXA−51-like was detected in all isolates, and bla OXA−23-like was identified in 92.5% of isolates. However, bla OXA−143-like and bla OXA−58-like genes were not detected among isolates. Four distinct bla OXA−51-like alleles were determined as follows: bla OXA−317 (67.0%), bla OXA−90 (9.4%), bla OXA−69 (17.0%), and bla OXA−64 (6.6%) and four ampC (bla ADC) allele types including ampC-25 (6.6%), ampC-39 (9.4%), ampC-1 (17.0%), and bla ADC−88 (67.0%) were identified. MLST (Pasteur scheme) analysis revealed four ST types including ST136 (singleton), ST1 (CC1), ST25 (CC25), and ST78 (singleton) in 71, 18, 7, and 10 of A. baumannii strains, respectively. Five RAPD clusters including A (1.9%), B (26.4%), C (57.5%), D (7.5%), and E (1.9%) were characterized and 5 (4.7%) strains were found to be singletons. Conclusion The present study demonstrated that there was a high prevalence of bla OXA−23-like producing CRAB in the clinical setting. The majority of isolates belonged to ST136 (singleton). However, bla OXA−23-like producing multidrug resistant international clones including ST1, and emerging lineages (e.g. ST25 and ST78) were also identified. Interestingly, in this study ST2 was not detected.
International Journal of Enteric Pathogens, May 28, 2020
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Bulgarian Journal of Veterinary Medicine, 2020
Soil-associated invertebrates which are coprophagic are capable of acting as paratenic hosts for ... more Soil-associated invertebrates which are coprophagic are capable of acting as paratenic hosts for Toxocara species larvae eggs. The aim of the present study was to assess the role of Lumbricus terrestris earthworms as potential intermediate host for Toxocara species. A total of 240 earthworms were collected from the public places in four areas of Karaj, Alborz Province, Iran. The subjects of the study were digested to detect Toxocara species using microscopy and molecular analyses. Toxocara larvae and larval eggs were recovered from 1.7% (4/240) of the sampled earthworms. The recovery rate in Shah'Abbasi and Talaghani regions in the studied areas was higher than that of the other regions. In the mentioned area, 8.3% (4/48) of earthworms were revealed to be positive for Toxocara species by microscopic observation and only one was confirmed to be positive for Toxocara canis by polymerase chain reaction (PCR). This is the first molecular report of Toxocara canis infective larvae in Lumbricus terrestris earthworms that could be demonstrated in common environment.
BMC Infectious Diseases, May 3, 2023
Background Toxocara infection is one of the most common neglected infections of poverty and a hel... more Background Toxocara infection is one of the most common neglected infections of poverty and a helminthiasis of global importance. Traditional diagnostic methods such as antibodies detection in serum samples are limited due to cross-reactivity and poor sensitivity. The use of molecular base methods for diagnosis of Toxocara infection in Iran has not been fully explored. The purpose of the current study was to estimate the prevalence of Toxocara infection from serum samples of people living with HIV in Alborz province, Iran using serological and molecular methods. Methods Blood samples were collected from 105 people living with HIV. Epidemiological data of participant were obtained through a structured questionnaire to investigate the risk factors. Patients CD4 + T cell count were recorded. Anti-Toxocara IgG antibodies were detected by ELISA, with a cutoff point of 11. PCR was performed to detect genetic material of Toxocara species in the serum samples. Results The mean CD4 + count in HIV-infected individuals with positive toxocariasis serology was 255.1 ± 21.6 cells/µL. Seropositivity for Toxocara species was observed in 12/105 (11.4%) people living with HIV. Three samples gave positive results on PCR analysis. Based on the data, a statistically significant relationship was found between anti-Toxocara IgG antibodies seropositivity and underlying conditions (p = 0.017). No significant statistical association was observed between seropositivity for Toxocara and gender, age, exposure to domestic animals or pet keeping, education levels, and occupation (p > 0.05). The findings of PCR confirmed Toxocara DNA in 3/12 (25.0%) serum samples. Conclusion These findings demonstrated for the first time that people living with HIV from Alborz province, are being exposed to this zoonosis and a relatively high seroprevalence of Toxocara in HIV/AIDS people needs comprehensive health education regarding personal hygiene and how to avoid exposure to this parasite infection, especially in people with an impaired immune system.
International Journal of Enteric Pathogens, Sep 23, 2017
Background Paper currencies are exchanged by persons with different hygiene habits, and are often... more Background Paper currencies are exchanged by persons with different hygiene habits, and are often stored under improper hygienic conditions. Although credit cards have replaced cash exchange in some cases, currency notes are still commonly used all over the world for the purchase of services and materials. 1 Paper banknotes have a large surface area for bacterial attachment and would be a vector for transmission of potentially pathogenic microorganisms between population. 2 The risk of microbial transfer by paper currency is influenced by factors such as paper value and duration of usage. 3 It is documented that lower value paper notes commonly carry the higher number of bacterial contamination because they are circulated more than higher denomination notes especially among the people with special occupation including beggars, school children, butchers and so on. 4 Recent studies showed that currency papers were contaminated by nematodes, protozoa, fungi and specially bacteria. 5-7 Several studies in different countries showed high rates of microbial contamination among paper currencies in circulation. 8-10 For example, all currency papers from India, Ghana, Bangladesh and Iraq were found to carry pathogenic or potentially pathogenic bacteria. 9-13 Moosavy et al showed that the most prevalent bacterial isolates from paper currencies were Escherichia coli, Staphylococcus aureus and Bacillus cereus. 14 In a study, it was revealed that coagulase-negative staphylococci, including Staphylococcus saprophyticus, Staphylococcus epidermidis and Staphylococcus hominis were the most
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Papers by Mohammad Zibaei