Papers by Mohamed Motawei
Arabian Journal for Science and Engineering
Seed Science and Technology, 2021
Buffelgrass (Cenchrus ciliaris) is widely grown in tropical and sub-tropical arid rangelands arou... more Buffelgrass (Cenchrus ciliaris) is widely grown in tropical and sub-tropical arid rangelands around the globe because of its high tolerance to drought and high level of nutrients. In the present study, the seed germination response of 12 buffelgrass genotypes (nine collected accessions and three introduced varieties) was tested to record the various effects of storage duration and temperature treatments on germination. Five constant temperature treatments (15, 20, 25, 30 and 35°C) and two storage durations (12 and 24 months) were used. The studied buffelgrass genotypes responded differently to the different storage durations and temperature regimes, suggesting the importance of genotype assessment under different environments. The highest germination percentages were recorded for genotype BUR-1 at 20°C (45%) after 12 months. No germination occurred after 12 months at low constant temperatures (15°C) for all genotypes except BUR-1, BUR-2 and UIA. Therefore, there is a large genetic v...
Molecular Biology Reports, 2021
Rusts are a group of major diseases that have an adverse effect on crop production. Those targeti... more Rusts are a group of major diseases that have an adverse effect on crop production. Those targeting wheat are found in three principal forms: leaf, stripe, and stem rust. Leaf rust causes foliar disease in wheat; in Egypt, this causes a significant annual yield loss. The deployment of resistant genotypes has proved to be a relatively economical and environmentally sustainable method of controlling the disease. Gene pyramiding can be performed using traditional breeding techniques. Additionally, pathotypes can be introduced to examine specific leaf rust genes, or the breeder may conduct more complex breeding methods. Indirect selection via DNA markers linked to resistance genes may facilitate the transfer of targeted genes, either individually or in combination, even in a disease-free environment. The use of selective crosses to counter virulent races of leaf, stripe, and stem rust has resulted in the transfer of several resistance genes into new wheat germplasm from cultivated or wild species. Quantitative trait locus (QTL) technology has been adopted in a wide variety of novel approaches and is becoming increasingly recognized in wheat breeding. Moreover, several researchers have reported the transference of leaf and stripe rust resistance genes into susceptible wheat cultivars.
Wheat cultivar ‘Yeocra Rojo’ and ten selected genotypes originating from immature embryo culture ... more Wheat cultivar ‘Yeocra Rojo’ and ten selected genotypes originating from immature embryo culture of this cultivar were evaluated for their productivity in two field experiments during 2005/2006 and 2006/2007 winter seasons. Wheat genotypes YR-9 and YR-10 exhibited the tallest plants whereas the shortest plants were those of cv. ‘Yeocra Rojo’. In general, genotypes YR-2, YR-7, and YR-10 produced the highest grain yield while YR-8 produced the lowest. YR-8 also gave the lowest harvest index. YR-7 had the most grains per spike, spike length and number of spikelets per spike compared to its parent cultivar and all other genotypes. A set of PCR-based markers for specific HMW glutenin genes encoding By-subunits were used to identify wheat genotypes carrying By genes at the Glu-B1 locus for its breadmaking quality. The presence of the gene encoding By8, which exists in the allele combination Glu-B1b (Bx7 + By8), was detected only in one genotype: YR-7. Primer pair ZSBy9aF1/R3 gave characte...
Genetika
Stress induced by high temperature represents a major constraint over wheat production in many pr... more Stress induced by high temperature represents a major constraint over wheat production in many production areas. Here, the comprehensive coverage of the wheat genome achievable using single nucleotide polymorphism markers was exploited to carry out a genetic analysis targeting yield components in plants exposed to high temperature stress. The mapping population was a set of doubled haploid lines derived from a cross between the cultivars Yecora Rojo and Ksu106. Both of the parental cultivars and their derived population were tested in the field in two locations over two consecutive seasons; at each site, two sowing dates were included, with the later sowing intended to ensure that the plants were exposed to high temperature stress during the grain filling period. Composite interval mapping detected 93 quantitative trait loci influencing grain yield and some related traits, along with 20 loci associated with a ?heat susceptibility index? (HSI). The loci were distributed over all 21 o...
NOVEMBER 2020
Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely in... more Buffelgrass (Cenchrus ciliaris L. Link) is an African agamospermous grass that has been widely introduced into arid regions of the world to improve rangelands for cattle production and as a result, it has invaded adjacent habitats. Cenchrus ciliaris is apomictic, a condition that is normally associated with low genetic variation within populations, but even moderate levels of genetic variation among populations could account for differences in invasiveness. We used Inter-Simple Sequence Repeat markers (ISSRs) to examine genetic variation among buffelgrass genotypes collected from nine different locations in the central region of Saudi Arabia, and three introduced cultivars. The genetic similarity coefficients among the twelve buffelgrass genotypes ranged from 0.66 to 0.94. Maximum similarity was observed between local populations. Introduced cultivars 'Biloela', 'Gayndah' and 'USA' were quite distinct from local populations. The dendogram constructed with UPG...
Saudi Journal of Biological Sciences, 2021
Wheat is an important cereal crop, and its significance is more due to compete for dietary produc... more Wheat is an important cereal crop, and its significance is more due to compete for dietary products in the world. Many constraints facing by the wheat crop due to environmental hazardous, biotic, abiotic stress and heavy matters factors, as a result, decrease the yield. Understanding the molecular mechanism related to these factors is significant to figure out genes regulate under specific conditions. Classical breeding using hybridization has been used to increase the yield but not prospered at the desired level. With the development of newly emerging technologies in biological sciences i.e., marker assisted breeding (MAB), QTLs mapping, mutation breeding, proteomics, metabolomics, next-generation sequencing (NGS), RNA_sequencing, transcriptomics, differential expression genes (DEGs), computational resources and genome editing techniques i.e. (CRISPR cas9; Cas13) advances in the field of omics. Application of new breeding technologies develops huge data; considerable development is...
Molecular Biology Reports
Cereal Research Communications
Cereal Research Communications
Journal of Plant Nutrition
November 2019
Six bread wheat genotypes were evaluated in three separate irrigation regime experiments to compa... more Six bread wheat genotypes were evaluated in three separate irrigation regime experiments to compare the response of agronomic performance and to identify genotypes with high yield potential under drought stress. The first irrigation treatment (I3) was given normal water irrigation (about 7000 m3 ha-1, according to recommendation for Qassim Region). The second (I2) and third (I1) treatments were given 2/3 and 1/3 of water amount of the first treatment, respectively. Factorial experiments in randomized complete block design with three replications were conducted during 2009/2010 and 2010/2011 seasons in the arid environment of central region of Saudi Arabia. Measurements were taken on days to heading, plant height, number of spikes m-2, number of kernels spike-1, 1000-kernel weight and grain yield. The drought susceptibility index (DSI) and water utilization efficiency (WUE) were calculated. The results revealed that effect of irrigation regime was highly significant for all traits, e...
Bulletin of the National Research Centre
Background Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and nat... more Background Cucurbita spp. is a main source of crypto-xanthine, zeaxanthin lutein folates, and natural poly-phenolic flavonoid compounds. Collection and conservation of genetic variability are helpful in genetic advancement programs. Twenty-two pumpkin genotypes (21genotypes of Cucurbita pepo L. and one genotype of C. maxima L.) were collected from different regions of Saudi Arabia. Fifteen HFO-TAGhigh frequency oligonucleotide–targeting active gene markers were used to analyze genetic variability among 22 pumpkin genotypes. Results A total of 107 alleles were detected by the 15 HFO-TAG markers, an average of 7.133 alleles per primer. Polymorphisms were found in 102 alleles, an average of 6.866 alleles per primer. The PIC values measured from all of the HFO-TAG markers were high, and ranged from 0.8940 to 0.7225, with an average 0.8212 per marker. Conclusions The results of the cluster analysis of pumpkin genotypes were separated into seven groups according to the collection region.
APRIL 2019
Low-fertility soil is considered a major challenge for growing wheat (Triticum aestivum) in organ... more Low-fertility soil is considered a major challenge for growing wheat (Triticum aestivum) in organic systems. The objective of this study was to identify spring wheat genotype(s) adapted to growing in organic systems with low-fertility soil compared with conventional systems in the central region of Saudi Arabia. Organic and conventional trials were established during the 2010, 2011 and 2012 growing seasons in the arid environment of central region of Saudi Arabia. Both trails were seeded in a sandy loam soil. Eight bread wheat genotypes were evaluated for the following parameters: grain and straw yields, grain-filling rate (GFR), days to heading (DTH), days to maturity (DTM), number of kernels per spike and 1000-kernel weight. The experiment was a randomized complete block design (RCBD) with four replications. The findings showed that there were significant differences in grain and straw yields between the two systems and among genotypes. The Egyptian genotypes 'Sids 12' and...
Journal of Agricultural and Marine Sciences [JAMS]
Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplif... more Genetic diversity of 10 potato cultivars was investigated at the DNA level with the random amplified polymorphic DNA (RAPD) procedure and at the phenotypic level using morphological characters. The results indicated that there were considerable variations among the different studied cultivars. Cultivars Victoria, Frizia and Safaren had the highest chlorophyll content compared to the other cultivars in both seasons. The largest leaf area and leaf dry weight were measured in cultivar Safaren and Mondial in both seasons. Cultivars Aboulx and Mondia produced the highest tuber yield in both seasons. On the other hand, cultivars Victoria and Edward were characterized by their considerably lower yield than other studied cultivars. Specific gravity varied considerably among the different potato cultivars. Thirteen random decamer primers were used to amplify DNA via the polymerase chain reaction and 75 RAPDs were generated. The RAPD profiles obtained were successfully used to differentiate p...
Journal of Agricultural and Marine Sciences [JAMS]
The suitability of randomly amplified polymorphic DNA (RAPD) fingerprints as genetic markers in d... more The suitability of randomly amplified polymorphic DNA (RAPD) fingerprints as genetic markers in date palms was tested. Five date palm cultivars (Barbi, Nabtet Ali. Rothanah, Ajwa, and Sokkari) from Saudi well- known dates were subject to DNA fingerprint analysis. From 20 primers tested, only 12 were selected as reproducible, giving 64 bands. The RAPD profiles obtained were successfully used to differentiate the genotypes. Based on the pair-wise comparison of amplification products, the genetic similarity was estimated. The five date palm cultivars showed variation at the DNA level. The genetic similarity among all date palm cultivars ranged from 70 to 85%. Sokkary was quite distant from Haiti and Ajwa cultivats. A dendrogram was constructed using UPGMA analysis. On the basis of this analysis, the populations were clustered into two clusters: cluster l contained Barhi and Ajwa cultivars, and cluster II contained Nabtet Ali, Rothanah and Sokkari cultivars. Therefore, the polymorphism ...
Electronic Journal of Biotechnology
African Journal of Biotechnology, 2012
This study was focused on studying the genetic diversity among pasture grass cultivars using inte... more This study was focused on studying the genetic diversity among pasture grass cultivars using intersimple sequence repeats (ISSR) markers to determine their genetic background. Six grass cultivars were used in this study: two perennial ryegrass (Lolium perenne L.) cultivars (Aries and Quartet), two endophyte-free tall fescue (Festuca arundinacea Schreb.) cultivars (Fawn and K5666v) and two orchardgrass (Dactylis glomerata L.) cultivars (Tekapo and Niva). Analysis of the 15 selected ISSR primers among the six grass cultivars generated 77 bands, 66 (85.7%) of which were polymorphic. The two perennial ryegrasses cultivars (Aries and Quartet) were distinguished by a 600-bp amplification fragment produced by primer UBC807. The 500-bp amplification fragment was produced by primer UBC825, which was distinguished in Niva, but absent in Tekapo-orchard grass. Furthermore, Fawn-tall fescue was distinguished by a 400-bp amplification fragment produced by primer UBC825. The minimum genetic similarity (GS) value was obtained between the two orchard grass cultivars (Niva and Tekapo), while the maximum GS value was obtained between the two perennial ryegrasses cultivars (Aries and Quartet).
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Papers by Mohamed Motawei