A high-affinity folate binding protein in proximal tubule cells of human kidney. High-affinity 3H... more A high-affinity folate binding protein in proximal tubule cells of human kidney. High-affinity 3H-folate binding in solubilized brush border membranes of human kidney cortex display characteristics such as apparent positive cooperativity typical of specific folate binding. The folate binding activity and the activity of the brush border membranemarker enzyme, gammaglutamyltransferase, were eightfold higher in brush border membranes compared to crude kidney homogenate. UltrogeiR AcA 44 chromatography revealed a major (Mr-100 kDa) and
Primary fibromyalgia syndrome (PFS) is a non‐articular rheumatic condition characterized by chron... more Primary fibromyalgia syndrome (PFS) is a non‐articular rheumatic condition characterized by chronic muscular pain. We have performed screening for autoantobodies in 20 women with PFS and in 19 age‐matched healthy women. Fifty‐five percent of the PFS patients had anti‐smooth muscle antibodies and 40% had anti‐striated muscle antibodies. None of the control subjects had any muscle antibodies. There was no significant difference in frequency of the remaining autoantibodies between the groups investigated. The present study indicates autoimmune responses in PFS against antigens of the diseased tissue itself, a finding which may be secondary to the disease or have relevance to the still obscure pathogenesis of the syndrome.
Rabbit antibodies to purified folate binding protein from cow's milk whey were used for devel... more Rabbit antibodies to purified folate binding protein from cow's milk whey were used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for quantitation of bovine folate binding proteins, The folate binding proteins in human milk and serum showed no cross-reactivity. A partial saturation of purified bovine folate binder with folate gave rise to an increased antigenicity probably due to a ligand (folate)-induced exposure of antigenic sites on the protein.
A high-affinity folate binding protein in proximal tubule cells of human kidney. High-affinity 3H... more A high-affinity folate binding protein in proximal tubule cells of human kidney. High-affinity 3H-folate binding in solubilized brush border membranes of human kidney cortex display characteristics such as apparent positive cooperativity typical of specific folate binding. The folate binding activity and the activity of the brush border membranemarker enzyme, gammaglutamyltransferase, were eightfold higher in brush border membranes compared to crude kidney homogenate. UltrogeiR AcA 44 chromatography revealed a major (Mr-100 kDa) and
Primary fibromyalgia syndrome (PFS) is a non‐articular rheumatic condition characterized by chron... more Primary fibromyalgia syndrome (PFS) is a non‐articular rheumatic condition characterized by chronic muscular pain. We have performed screening for autoantobodies in 20 women with PFS and in 19 age‐matched healthy women. Fifty‐five percent of the PFS patients had anti‐smooth muscle antibodies and 40% had anti‐striated muscle antibodies. None of the control subjects had any muscle antibodies. There was no significant difference in frequency of the remaining autoantibodies between the groups investigated. The present study indicates autoimmune responses in PFS against antigens of the diseased tissue itself, a finding which may be secondary to the disease or have relevance to the still obscure pathogenesis of the syndrome.
Rabbit antibodies to purified folate binding protein from cow's milk whey were used for devel... more Rabbit antibodies to purified folate binding protein from cow's milk whey were used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for quantitation of bovine folate binding proteins, The folate binding proteins in human milk and serum showed no cross-reactivity. A partial saturation of purified bovine folate binder with folate gave rise to an increased antigenicity probably due to a ligand (folate)-induced exposure of antigenic sites on the protein.
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