Proceedings of the National Academy of Sciences, 2009
Directed differentiation of embryonic stem cells indicates that mesodermal lineages in the mammal... more Directed differentiation of embryonic stem cells indicates that mesodermal lineages in the mammalian heart (cardiac, endothelial, and smooth muscle cells) develop from a common, multipotent cardiovascular precursor. To isolate and characterize the lineage potential of a resident pool of cardiovascular progenitor cells (CPcs), we developed BAC transgenic mice in which enhanced green fluorescent protein (EGFP) is placed under control of the c-kit locus (c-kit BAC -EGFP mice). Discrete c-kit-EGFP + cells were observed at different stages of differentiation in embryonic hearts, increasing in number to a maximum at about postnatal day (PN) 2; thereafter, EGFP + cells declined and were rarely observed in the adult heart. EGFP + cells purified from PN 0–5 hearts were nestin + and expanded in culture; 67% of cells were fluorescent after 9 days. Purified cells differentiated into endothelial, cardiac, and smooth muscle cells, and differentiation could be directed by specific growth factors. ...
Proceedings of the National Academy of Sciences, 2009
Directed differentiation of embryonic stem cells indicates that mesodermal lineages in the mammal... more Directed differentiation of embryonic stem cells indicates that mesodermal lineages in the mammalian heart (cardiac, endothelial, and smooth muscle cells) develop from a common, multipotent cardiovascular precursor. To isolate and characterize the lineage potential of a resident pool of cardiovascular progenitor cells (CPcs), we developed BAC transgenic mice in which enhanced green fluorescent protein (EGFP) is placed under control of the c-kit locus (c-kit BAC -EGFP mice). Discrete c-kit-EGFP + cells were observed at different stages of differentiation in embryonic hearts, increasing in number to a maximum at about postnatal day (PN) 2; thereafter, EGFP + cells declined and were rarely observed in the adult heart. EGFP + cells purified from PN 0–5 hearts were nestin + and expanded in culture; 67% of cells were fluorescent after 9 days. Purified cells differentiated into endothelial, cardiac, and smooth muscle cells, and differentiation could be directed by specific growth factors. ...
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Papers by Michael Craven