Papers by Mehran Alamdari
Arsenic contamination of groundwater has been previously reported in Ghopuz, a village located in... more Arsenic contamination of groundwater has been previously reported in Ghopuz, a village located in the Northwest of Iran. Samples were taken from consuming and irrigation water and plants of the region for chemical analysis. A seven-year old ewe, which had lived in and fed a lifelong at the same place, with clinical signs such as weakness, wasting and inappropriate integument was necropsied. Grossly, buccal erosion, stomatitis, cutaneous ulcers and serous atrophy of fat deposits were observed. Rumen contents, wool and several tissue samples were obtained for toxicological and histopathological examinations. Mean arsenic concentration in the spring water, irrigation water and grass/algae were 70.11, 48.74 and 141.85 ppb (µg/kg), respectively. Arsenic levels were 486.73, 247.94, 127.92, 125.97 and 231.24 ppb in wool, skin, rumen contents, liver and kidney, respectively. Microscopic study revealed hyperemia and heavy parasitic infestation of the abomasal wall. Hyperemia and regeneration of renal tubule epithelia were observed in kidneys and hyperkeratosis, suppurative deep dermatitis and paniculitis were found in skin. Periacinar fibrosis and a poorly differentiated cholangiocarcinoma were seen in liver. In pancreas, reduced cell density of islands of Langerhans was noticeable. In the central nervous system, perineuronal and perivascular edema, ischemic changes in gray matter neurons, and microcavitation of white matter were present. Our findings confirmed chronic arsenic toxicosis in small ruminants in this region. It can be concluded that long-term consumption of arsenic contamined water and forage may be associated with chronic arsenic poisoning in domestic animals and human beings, with consequent neoplastic disease and induction of diabetes in this region.
ALAMDARI, M., S. A. GHORASHI, M. AHMADI, R. SALEHI TABAR: Detection of foot-and-mouth disease vir... more ALAMDARI, M., S. A. GHORASHI, M. AHMADI, R. SALEHI TABAR: Detection of foot-and-mouth disease virus and identifi cation of serotypes in East Azerbaijan province of Iran. Vet. arhiv 76, 413-419, 2006. ABSTRACT Three serotypes of foot-and-mouth disease virus (FMDV) including A, Asia1 and O, have been detected in Iran. Following the mass vaccination program which was implemented in all parts of the country in 2002, the number of outbreaks has been significantly reduced. Therefore, rapid detection of FMDV and its serotypes in clinical samples is essential for control of new outbreaks. In this study a reverse transcription-polymerase chain reaction (RT-PCR) was used for detection and serotyping of FMDV in clinical samples. Twelve tissue samples were collected from suspected outbreaks from East Azerbaijan province and tested by this method. Of 12 samples, 10 were found to be positive for FMDV. These samples were also tested in a multiplex-PCR for serotype identification. Four samples showed to be serotype O, 4 samples identified as serotype A and 2 samples detected to be serotype Asia 1. Multiplex-PCR products were sequenced and specificity of results was confirmed. Results indicate that in order to practice a good control measure, the RT-PCR and multiplex-PCR could be successfully used as a robust diagnostic method.
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Papers by Mehran Alamdari