Papers by Meera Srivastava
The pulse beetle Callosobruchus sp. (Coleoptera: Bruchidae), is a major pest of economically impo... more The pulse beetle Callosobruchus sp. (Coleoptera: Bruchidae), is a major pest of economically important leguminous grains, such as cowpeas), lentils, green gram, and black gram (Talukder & Howse, 1994; Park et al., 2003).As it is not advisable to mix insecticides with food grains the plant materials appear to be the most promising agents for grain protection against them. Euphorbiaceae is world wide in distribution and exposed to all sorts of habitats. A chemical review of the different classes of compounds which have been isolated from the Euphorbiaceae (other than the diterpenoids) has been given by Abdel-Fattah (1987). Members of this family have been reported to possess antibacterial (ElBassuony,2007; Ogbulie et al., 2007; Valente et al., 2004; Zakaria et al.,2006; Falodun et al., 2008; Awanchiri et al.,2009), anti-viral (Bedoya et al.,2009;,Bentacur-Galvis et al.,2002;Lin et al., 2002), anti-fungal (Cox et al.,2006; Devi and Gupta, 2000), nematicidal (Shakil et al.,2006;Shi et a...
Introduction In India, where the population is predominantly vegetarian, pulses are the most impo... more Introduction In India, where the population is predominantly vegetarian, pulses are the most important and rich source of protein and several amino acids. Besides, these also provide energy, minerals and certain vitamins. Pulses contain 20-30% of protein which is almost three times higher than cereals (Doharey et al., 1990). According to reports available, in India, over 200 species of insects have been recorded infesting various pulses (CABI, 2007). Of these, 17 species of bruchids belonging to 11 genera have been recorded infesting different pulses (Arora, 1977). The genus Callosobruchus attacks grain legumes during both pre and post harvest stages all over the world; but in India, C.maculatus, C. analis and C. chinensis are the predominant pest species of the genera (Dias, 1988). The bruchid infestation also affects seed quality, market value and can reduce cowpea seed viability to 2% after months of storage (Caswell, 1980).
UK Journal of Pharmaceutical Biosciences, 2016
Animals including camel (Camelus dromedaries), have been domesticated by man since ancient times ... more Animals including camel (Camelus dromedaries), have been domesticated by man since ancient times especially in Rajasthan. Due to harsh climatic conditions there is often scarcity of grazing pastures in this area and therefore during summers camels are fed roughages and concentrates, as there remains no option to the local farmers. As a result, the proportion of the concentrate and roughage in the complete ration is expected to change the microbial population in the rumen, which in turn is expected to affect their capacity to colonize feed particles and may influence the nutrient utilization from the feed. Cluster bean (guar) belonging to family Leguminosae is one of the most suitable feed in arid areas. Besides, camels are also fed some millet flour or barley flour and gur (molasses) 1g/kg body weight. If this molasses is given in excess amount, it causes gastro-intestinal disorders. Due to introduction of new feed resources, this study was an attempt to investigate certain blood an...
UK Journal of Pharmaceutical Biosciences, 2014
An in vitro trial was conducted in fermentation vessels under anaerobic conditions using composit... more An in vitro trial was conducted in fermentation vessels under anaerobic conditions using composite feed-1 and composite feed-2 with inclusion of different levels of Coconut Oil (CO) along with buffered rumen liquor of sheep to observe the effects of oil on rumen methanogenesis and fermentation. The Composite Feed-1(CF-1) consisted of 40% of concentrate and Composite Feed-2 (CF-2) had 25 % of concentrate. The different levels of CO used were 0.5,1 and 2 µl/ml. Among the gas production and methane concentration parameters the inclusion of CO showed a significant decrease of 38.67%. A non significant effect on gas production was noted with the inclusion of CO in CF-2. Further, a decrease in total gas production and methane concentration was observed which was highly pronounced in CF-1 as compared to CF-2. The rumen digestibility by inclusion of CO in CF-1 and CF-2 decreased significantly (P < 0.01) from control. The inclusion of CO in CF-2 did not affect Ammonia Nitrogen (NH3 –N) co...
Animal Biotechnology, 2016
Interleukin (IL)-1β and IL-8 are pro-inflammatory cytokines produced primarily by monocytes and m... more Interleukin (IL)-1β and IL-8 are pro-inflammatory cytokines produced primarily by monocytes and macrophages in response to a variety of microbial and nonmicrobial agents. As yet, no molecular data have been reported for IL-1β and IL-8 of the Asian elephant. In the present study, we have cloned and sequenced the cDNA encoding IL-1β and IL-8 of the Asian elephant. The open reading frame (ORF) of Asian elephant IL-1β is 789 bp in length, encoded a propeptide of 263 amino acid polypeptide. The predicted protein revealed the presence of IL-1 family signature motif and an ICE cut site. Whereas, IL-8 contained 321 bp of open reading frame. Interestingly, the predicted protein sequence of 106 aa, contains an ELR motif immediately upstream of the CQC residues, common in all vertebrate IL-8 molecules. Identity levels of the nucleic acid and deduced amino acid sequences of Asian elephant IL-1β ranged from 68.48 (Squirrel monkey) to 98.57% (African elephant), and 57.78 (Sheep) to 98.47% (African elephant), respectively, whereas that of IL-8 ranged from 72.9% (Human) to 87.8% (African elephant), and 63.2 (human, gorilla, chimpanzee) to 74.5% (African elephant, buffalo), respectively. The phylogenetic analysis based on deduced amino acid sequenced showed that the Asian elephant IL-1β and IL-8 were most closely related to African elephant. Molecular characterization of these two cytokines, IL-1β and IL-8, in Asian elephant provides fundamental information necessary to progress the study of functional immune responses in this animal and gives the potential to use them to manipulate the immune response as recombinant proteins.
Indian Journal of Animal Research, 2015
Trypanosomosis caused by Trypanosoma evansi is a disease of economic significance of domestic liv... more Trypanosomosis caused by Trypanosoma evansi is a disease of economic significance of domestic livestock, causing trypanosomiasis, commonly known as Surra. In the present study, gene of Trypanosoma evansi have beer cloned and sequenced. Beta-tubulin is important for cellular structure and physical functions. Sequence analysis of this gene will be useful for the vaccine studies. The open reading frame of -tubulin of Trypanosoma evansi is 1329 bp in length, encoding 442 amino acid polypeptide. The sequence analysis revealed that the ß tubulin gene of Trypanosoma evansi from India was having the identity of 99% with T.equiperdum, T.evansi (China isolate), T.evansi (Indian isolates), T.brucei and 88% with T.cruzi. The stable biological characteristics of Trypanosomatidae beta-tubulin make it an ideal target of many anti-parasitic drugs that block tubulin function.
International Journal of Scientific Research, 2012
Rhizopertha domonica is secondary pest of stored grains. Both the adults and grubs cause consider... more Rhizopertha domonica is secondary pest of stored grains. Both the adults and grubs cause considerable damage to the grains under storage in tropical and subtropical parts including India. Many synthetic organic pesticides are used to control stored grain pest which results in damage to the environment. In the last decade, however, there has been an increasing interest in natural products particularly those of plant origin to control pest species. In view of this, the smoke of different parts of the plants, Peganum harmala. Peganum harmala was used against the Rhizopertha domonica and adult mortality was recorded after 48 hours of exposure. 20 adult insects were subjected to the smoke of various plant parts in chamber specially designed for this purpose. It was observed that the smoke treatement of the leaf of Peganum harmala causes 40-50% mortality in Rhizopertha dominica after 48 hours of treatement.
International Journal of Scientific Research, 2012
The present research was carried out to evaluate the grain protectant efficacy of plant Tribulus ... more The present research was carried out to evaluate the grain protectant efficacy of plant Tribulus terrestris, different parts of which were formulated using different extracts and at various concentrations against Tribolium castaneum. The results showed that the damaged percentage of grains of Triticum vulgare infested with Tribolium castaneum at direct relationship with concentration. The sets treated with dose concentration of 10% were most effective and recorded lowest damage to the grains. Efficacy of Plant Tribulus Terrestris on The Percentage of Damaged Grains of Triticum Vulgare Infested by Beetle Tribolium Castaneum
International Journal of Scientific Research, 2012
Indian Journal of Applied Research, 2011
The study deals with evaluating insecticidal efficacy of a desert plant, Peganum harmala (family:... more The study deals with evaluating insecticidal efficacy of a desert plant, Peganum harmala (family: Zygophyllaceae), different parts of which were formulated using different extracts and at various concentrations against Tribolium castaneum. The results showed that the ether extracts at highest dose concentration of 10% were the most effective in increasing adult mortality, retarding rate of development, reducing egg laying, adult emergence and weight loss in host grains of Triticum vulgare. Among the plant parts the leaf was found to be most effective followed by fruit, root and stem.
International Journal of Scientific Research, 2012
The present research was carried out to evaluate the grain protectant efficacy of plant Peganum h... more The present research was carried out to evaluate the grain protectant efficacy of plant Peganum harmala, different parts of which were formulated using different extracts and at various concentrations against Callosobruchus chinensis. The results showed that the damaged percentage of grains of Vigna radiata infested with Callosobruchus chinensis have direct relationship with concentration. The sets treated with dose concentration of 10% were most effective and recorded lowest damage to the grains.
Indian Journal of Applied Research, 2011
Rhizopertha dominica is major pest of pulses and cereals. Both the adults and grubs cause conside... more Rhizopertha dominica is major pest of pulses and cereals. Both the adults and grubs cause considerable damage to the grains under storage in tropical and subtropical parts including India. The management of stored grain pest has been typically carried out synthetic organic pesticides which resulted in damage to the environment. In the last decade, however there has been an increasing interest in natural products, particularly those of plant origin to control the pest species. In view of this, the smoke of different parts (root, stem, leaf and fruit) of plant Aerva tomentosa was used against the pest R.dominica and adult mortality was recorded after 48 hours of exposure. 20 adult insects were subjected to the smoke of various plant parts in chambers specially fabricated for this purpose. It was observed that the smoke treatment of leaf of Aerva tomentosa causes 40-50% mortality in Rhizopertha after 48 hours of treatment.
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Papers by Meera Srivastava