Papers by Marina Mora Ortiz
Nutrients, 2024
Abstract: The literature on the postprandial metabolic changes in individuals with Metabolic Syn-... more Abstract: The literature on the postprandial metabolic changes in individuals with Metabolic Syn- drome (MetS) remains limited, despite the fact that postprandial states represent the most common physiological condition in Western societies. Background/Objectives: The objective of this study was to investigate the plasma metabolomics profile in both fasting and postprandial states following a high-fat challenge in individuals with MetS who consumed diets with varying quantities and qualities of dietary fat over 12 weeks. Methods: Seventy-five patients with MetS (28 males and 47 females) from the Spanish LIPGENE cohort were included in the study. MetS patients were randomly stratified to follow one of four dietary interventions (isoenergetic diets) for a 12-week long- term study. The four diets were high in saturated fatty acids and high in monounsaturated fatty acids (HSFA and HMUFA), low-fat high-complex carbohydrates (LFHCC), and LFHCC supplemented with n-3. The metabolomics analysis of plasma samples was carried out using Liquid Chromatography Time-of-Flight Mass Spectrometry (LC-TOF/MS). Results: We observed a decrease in inflamma- tion biomarkers, including acetylcarnitine and L-carnitine during the fasting state and hexanoyl-L- carnitine and isobutyryl-L-carnitine during the postprandial period, mediated by the replacement of HSFA with HMUFA. Additionally, antioxidant compounds such as 4-hydroxybenzaldehyde and L-valine were expressed at higher levels after consumption of the HMUFA diet compared to the HSFA diet. HSFA also presented altered levels of phosphatidylcholine, a metabolite previously linked with insulin resistance. Conclusions: These findings suggest that replacing HSFA with HMUFA may reduce inflammation and improve antioxidant profiles, supporting the potential for tailored dietary interventions in individuals with MetS.
Colonoscopy inferential statistics. Boxplots in panels from left to right show the traits: total ... more Colonoscopy inferential statistics. Boxplots in panels from left to right show the traits: total number of abnormalities in mucosa, number of polyps and /or diverticulosis location, total number of polyps and total number of diverticulosis locations. The top row shows effects in age, and the bottom one in BMI. Frailty was not found significant.<b></b>
Cohort distribution. Panel a) Histograms with population distribution for age, BMI and frailty (f... more Cohort distribution. Panel a) Histograms with population distribution for age, BMI and frailty (from left to right). Panel b) Line chart pairing twin one (lowest value for each trait) with twin two (highest value for the trait). Steep lines indicate discordancy between traits. These traits are, from left to right: socioeconomical status, BMI and frailty. Panel c) Pie charts showing percentages of individuals belonging to each class of the phenotypes: rearing, ethnicity, sex, area where are they living, whether or not the visited together the colonoscopy appointment, smoking status, whether or not they are living in the same county and socio-economic status (SES).
<b>Eligibility criteria for</b><b>TwinsUK ExHiBITT</b>
Summary of trimming of sequencing reads. Table with summary of the trimming of the sequencing rea... more Summary of trimming of sequencing reads. Table with summary of the trimming of the sequencing reads. (DOCX 12Â kb)
SSRs identified in the sainfoin combined assembly. Excel file with list of SSR motifs identified ... more SSRs identified in the sainfoin combined assembly. Excel file with list of SSR motifs identified in transcripts from Sainfoin Transcriptome 1.0. (XLSX 498Â kb)
Biology of Sex Differences
Metabolic diseases, such as obesity, metabolic syndrome (MetS) and type 2 diabetes (T2D), are now... more Metabolic diseases, such as obesity, metabolic syndrome (MetS) and type 2 diabetes (T2D), are now a widespread pandemic in the developed world. These pathologies show sex differences in their development and prevalence, and sex steroids, mainly estrogen and testosterone, are thought to play a prominent role in this sexual dimorphism. The influence of sex hormones on these pathologies is not only reflected in differences between men and women, but also between women themselves, depending on the hormonal changes associated with the menopause. The observed sex differences in gut microbiota composition have led to multiple studies highlighting the interaction between steroid hormones and the gut microbiota and its influence on metabolic diseases, ultimately pointing to a new therapy for these diseases based on the manipulation of the gut microbiota. This review aims to shed light on the role of sexual hormones in sex differences in the development and prevalence of metabolic diseases, f...
BMC Medicine
Background Type 2 diabetes mellitus (T2DM) is one of the most widely spread diseases, affecting a... more Background Type 2 diabetes mellitus (T2DM) is one of the most widely spread diseases, affecting around 90% of the patients with diabetes. Metabolomics has proven useful in diabetes research discovering new biomarkers to assist in therapeutical studies and elucidating pathways of interest. However, this technique has not yet been applied to a cohort of patients that have remitted from T2DM. Methods All patients with a newly diagnosed T2DM at baseline (n = 190) were included. An untargeted metabolomics approach was employed to identify metabolic differences between individuals who remitted (RE), and those who did not (non-RE) from T2DM, during a 5-year study of dietary intervention. The biostatistical pipeline consisted of an orthogonal projection on the latent structure discriminant analysis (O-PLS DA), a generalized linear model (GLM), a receiver operating characteristic (ROC), a DeLong test, a Cox regression, and pathway analyses. Results The model identified a significant increase...
Panel a) Study design included the recruitment of at least 200 twin volunteers. All samples were ... more Panel a) Study design included the recruitment of at least 200 twin volunteers. All samples were taken on the day of the visit except for the stool posted sample that was donated one week after. Panel b) Samples taken on the visit included colon biopsies, blood, saliva and caecal fluid. Panel c) Different factors can interact with the host immune system, among them the microbiota. Some of the mechanisms previously described involved allergies, Type 1 Diabetes (T1D), Rheumatoid Arthritis (RA), Multiple Sclerosis (MS) and Inflammatory Bowel Disease (IBS). <i>Panel c) was adapted from </i><i>(12)</i>.
Concordant twin pairs for: a) diverticulosis and b) polyps. c) Twin pair where one twin had a pot... more Concordant twin pairs for: a) diverticulosis and b) polyps. c) Twin pair where one twin had a potential cancer and twin 2 had more had 7 polyps.
SNP polymorphisms in the five sainfoin transcript assemblies. A csv file listing putative SNP var... more SNP polymorphisms in the five sainfoin transcript assemblies. A csv file listing putative SNP variants identified in one or more of the five sainfoin transcriptome assemblies. (CSV 6315Â kb)
Experimentally validated SNPs. Excel file summarising the experimentally validated SNPs. (XLSX 11... more Experimentally validated SNPs. Excel file summarising the experimentally validated SNPs. (XLSX 11Â kb)
Transcriptomics assembly. FASTA file of 92772 sainfoin transcripts. (ZIP 26489Â kb)
Annotation of the sainfoin assembly. Excel file with list of annotated contigs from Sainfoin Tran... more Annotation of the sainfoin assembly. Excel file with list of annotated contigs from Sainfoin Transcriptome 1.0. Contig names in green indicate transcripts encoding genes involved in the flavonoid biosynthesis pathway. Contigs in blue indicate transcripts with potential involvement in proanthocyanidin biosynthesis. (XLSX 1685Â kb)
Tannins are a diverse group of plant phenolic compounds. Condensed tannins (CTs) represent a majo... more Tannins are a diverse group of plant phenolic compounds. Condensed tannins (CTs) represent a major subgroup of tannins and were extracted from tilia (Tilia L.) flowers and black locust (Robinia pseudoacacia) leaves. These extracts were examined for their effects on the metabolic profile of chicken caeca. By using in vitro, a nuclear magnetic resonance (1H-NMR), which was combined with multivariate statistics, the current study was applied for the first time to investigate how three different CT compositions, procyanidins (PC) and/or prodelphinidins (PD) units influenced the metabolic end-products in caecal contents of chickens. In the presence of tannins, glutamate, leucine, lysine, pyroglutamate, phenylalanine, proline, and sarcosine were significantly decreased. CT extracts significantly influenced the fermentation, increasing the concentrations of some fatty acids such as acetate, butyrate, and propionate whereas. In contrast, lactate decreased between the treatments. This study ...
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Papers by Marina Mora Ortiz