Papers by Margareth Ribeiro Moysés
Anais Brasileiros De Dermatologia, Dec 1, 2005
PP016-Utilização da terapia ultra-sônica de baixa intensidade na redução da lipodistrofia ginecói... more PP016-Utilização da terapia ultra-sônica de baixa intensidade na redução da lipodistrofia ginecóide: uma terapia segura ou risco cardiovascular transitório?-Um estudo pré-clínico * Use of low intensity ultrasonic therapy in the reduction of gynecoid lipodystrofy: a safe therapy or transitory cardiovascular risk?-A pre-clinical study *
Basic & Clinical Pharmacology & Toxicology, Jul 6, 2011
Raloxifene is a selective oestrogen receptor modulator that has been approved for the prevention ... more Raloxifene is a selective oestrogen receptor modulator that has been approved for the prevention and treatment of osteoporosis in post-menopausal women. Studies have revealed several effects of raloxifene on the cardiovascular system, which might contribute to the blood pressure regulatory mechanisms, particularly in the systemic arterial hypertension. Therefore, the aim of this study was to investigate the effects of raloxifene on the blood pressure, renal excretion of water and Na(+) and plasma nitrite/nitrate levels in 2-kidney-1-clip (2K1C) hypertensive female rats. The groups were as follows: hypertensive (2K1C), hypertensive ovariectomized (2K1C + OVX) and hypertensive ovariectomized treated with raloxifene (2K1C + OVX + R). Seven days after the surgery that produced menopause, 2K1C hypertension was produced in anaesthetized animals. Seven days after the clip application, the rats were put into metabolic cages to allow for the measurement of water ingestion and diuresis, and raloxifene was administered (2 mg/kg/day i.p., for 7 more days). We found a large reduction (p < 0.01) in mean arterial pressure (197 ± 6 to 164 ± 2 mmHg), an increase in renal excretion of sodium and water (p < 0.05) and an increase in plasma levels of nitrite/nitrate in 2K1C + OVX + R animals, when compared with the 2K1C (23.4 ± 1 versus 14 ± 0.5 nmol/mL; p < 0.01, respectively). These findings suggest that raloxifene exerted its antihypertensive effect, at least in part, by improving the renal excretion of sodium and water.
Brazilian Journal of Medical and Biological Research, Apr 1, 2004
The present study was designed to determine relaxation in response to 17ß-estradiol by isolated p... more The present study was designed to determine relaxation in response to 17ß-estradiol by isolated perfused hearts from intact normotensive male and female rats as well as the contribution of endothelium and its relaxing factors to this action. Baseline coronary perfusion pressure was determined and the vasoactive effects of 17ß-estradiol (10 µM) were assessed by in bolus administration before and after endothelium denudation by infusion of 0.25 µM sodium deoxycholate or perfusion with 100 µM L-NAME, 2.8 µM indomethacin, 0.75 µM clotrimazole, 100 µM L-NAME plus 2.8 µM indomethacin, and 100 µM L-NAME plus 0.75 µM clotrimazole. Baseline coronary perfusion pressure differed significantly between males (84 ± 2 mmHg, N = 61) and females (102 ± 2 mmHg, N = 61). Bolus injection of 10 µM 17ßestradiol elicited a transient relaxing response in all groups, which was greater in coronary beds from females. For both sexes, the relaxing response to 17ß-estradiol was at least in part endothelium-dependent. In the presence of the nitric oxide synthase inhibitor L-NAME, the relaxing response to 17ß-estradiol was reduced only in females. Nevertheless, in the presence of indomethacin, a cyclooxygenase inhibitor, or clotrimazole, a cytochrome P450 inhibitor, the 17ßestradiol response was significantly reduced in both groups. In addition, combined treatment with L-NAME plus indomethacin or L-NAME plus clotrimazole also reduced the 17ß-estradiol response in both groups. These results indicate the importance of prostacyclin and endothelium-derived hyperpolarizing factor in the relaxing response to 17ß-estradiol. 17ß-estradiol-induced relaxation may play an important role in the regulation of coronary tone and this may be one of the reasons why estrogen replacement therapy reduces the risk of coronary heart disease in postmenopausal women.
Universidade Federal do Espírito Santo, Aug 1, 2018
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1988
The contractile reactivity to norepinephrine, methoxamine, and verapamil of the perfused mesenter... more The contractile reactivity to norepinephrine, methoxamine, and verapamil of the perfused mesenteric vascular bed from sinoaortic denervated (SAD) and sham-operated (SO) rats was studied 3 to 30 days after surgery. A gradual but incomplete reduction of arterial hypertension was observed in SAD rats throughout the study. The norepinephrine- and methoxamine-induced dose-response curves were similar in both SAD and SO groups on day 3, but shifted to the left on days 7 and 15 and demonstrated a tendency to shift to the right at 30 days. Verapamil-induced vasodilation was similar in both groups. Enhanced mesenteric vascular responsiveness to endogenous catecholamines could contribute to the increased vascular resistance.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 1988
In a previous study we demonstrated ventricular hypertrophy in male but not in female sinoaortic ... more In a previous study we demonstrated ventricular hypertrophy in male but not in female sinoaortic denervated rats. To evaluate a possible sexual influence on ventricular hypertrophy of other models for experimental hypertension, we studied two-kidney one clip and one-kidney one clip Goldblatt hypertension. The results showed left ventricular hypertrophy in male but not in female two-kidney one clip groups, despite the same arterial hypertension level in both groups. In the one-kidney one clip groups, left ventricular hypertrophy was greater in male than in female rats. The results indicate a sexual influence in ventricular hypertrophy when the arterial renovascular hypertension level is moderate.
Pharmacological Research Communications, 1988
Alterations in vascular alpha-adrenoceptor responsiveness following sinoaortic denervation was st... more Alterations in vascular alpha-adrenoceptor responsiveness following sinoaortic denervation was studied in conscious rats. The arterial hypertension observed in baroreceptor denervated rats decreased progressively during the 30 days of observation. A pressor hyperresponsiveness to methoxamine (10-80 ~g/kg, iv), was observed 3 to 7 days after baroreceptor denervation followed by a gradual normalization of the vascular reactivity. The results indicate a possible participation of an enhanced alphal-adrenoceptor-mediated vasoconstrictor component in the early phase of neurogenic hypertension.
Jornal de Pediatria, 2009
Objective: To evaluate the occurrence of metabolic syndrome (MS) and independent associated risk ... more Objective: To evaluate the occurrence of metabolic syndrome (MS) and independent associated risk factors in adolescents in the city of Vitória, Brazil. Methods: We assessed 380 adolescents aged 10 to 14 years attending public schools. Body mass index and blood pressure at rest were measured. Fasting plasma concentrations of total cholesterol, LDL-cholesterol, HDL-cholesterol, triglycerides and glucose were also obtained. Results: The prevalence of overweight was 9.6% for boys and 7.4% for girls, while obesity was found in 6.2 and 4.9%, respectively. Triglyceride concentrations were borderline or high in 6.8 and 3.4% of the boys and in 11.8 and 5.9% of the girls. HDL-cholesterol was below recommended levels in 8.5% of the boys and in 9.9% of the girls. Blood pressure at rest was borderline for 5.1% of the boys and 7.9% of the girls, while 3.4% of both boys and girls were hypertensive. Fasting glycemia was high in 0.6% of the boys and in 0.5% of the girls. In the group studied, 2.8% of the boys and 2.5% of the girls had two risk factors associated with MS. Prevalence of MS was 1.1% for boys and 1.5% for girls, and overall prevalence was 1.3%. Conclusions: MS and associated cardiovascular risk factors are serious clinical conditions in this age group. A significant number of adolescents showed borderline results, which may increase the prevalence of MS or independent risk factors in the short term. More investments should be made in primary prevention, considering that early diagnosis is an issue of fundamental importance.
PLoS ONE, 2013
The aim of this study was to evaluate the effects of swimming training (SW) and oestrogen replace... more The aim of this study was to evaluate the effects of swimming training (SW) and oestrogen replacement therapy (ERT) on coronary vascular reactivity and the expression of antioxidant enzymes in ovariectomized rats. Animals were randomly assigned to one of five groups: sham (SH), ovariectomized (OVX), ovariectomized with E2 (OE2), ovariectomized with exercise (OSW), and ovariectomized with E2 plus exercise (OE2+SW). The SW protocol (56/week, 60 min/day) and/or ERT were conducted for 8 weeks; the vasodilator response to bradykinin was analysed (Langendorff Method), and the expression of antioxidant enzymes (SOD-1 and 2, catalase) and eNOS and iNOS were evaluated by Western blotting. SW and ERT improved the vasodilator response to the highest dose of bradykinin (1000 ng). However, in the OSW group, this response was improved at 100, 300 and 1000 ng when compared to OVX (p,0,05). The SOD-1 expression was increased in all treated/trained groups compared to the OVX group (p,0,05), and catalase expression increased in the OSW group only. In the trained group, eNOS increased vs. OE2, and iNOS decreased vs. SHAM (p,0,05). SW may represent an alternative to ERT by improving coronary vasodilation, most likely by increasing antioxidant enzyme and eNOS expression and augmenting NO bioavailability.
Pharmacology, 1988
The effects of nifedipine (15 micrograms/kg, i.v.) on mean blood pressure (MBP) and heart rate we... more The effects of nifedipine (15 micrograms/kg, i.v.) on mean blood pressure (MBP) and heart rate were studied in neurogenic hypertensive rats (NHR), compared with renovascular hypertensive (RHR), and control (CR) rats. The maximal MBP reduction reached 32, 37 and 9%, respectively. The baseline MBP was recovered after 120, 150, and 5 min, respectively. The hypotension was not accompanied by changes in the baseline high heart rate of NHR and by tachycardia in CR and RHR groups. The data show the antihypertensive efficacy of nifedipine in both models of hypertension and indicate that under conditions of increased sympathetic tone the cardiac pacemaker cannot be easily inhibited by nifedipine.
Clinical and Experimental Hypertension, 2004
The renin-angiotensin system plays a role in the pathophysiology of renovascular hypertension. In... more The renin-angiotensin system plays a role in the pathophysiology of renovascular hypertension. In addition, some studies have demonstrated a beneficial effect of L-arginine (L-Arg), the precursor of nitric oxide (NO), in this model of hypertension. This study was designed to investigate the effects of L-Arg on cardiovascular parameters and on the activity of the angiotensin-converting enzyme (ACE), after 14 days of renovascular hypertension. The experiments were performed on conscious male Wistar rats. Two-kidney, one-clip renovascular hypertension (2KIC) was initiated in rats by clipping the left renal artery during 14 days, while control rats were sham-operated. One group was submitted to a similar procedure and treated with L-Arg (10 mg/ml; average intake of 300mg/day) from the 7th to the 14th day after surgery, whereas the respective control group received water instead. At the end of the treatment period, the mean arterial pressure (MAP) was measured in conscious animals. The rats were sacrificed and the ACE activity was assayed in heart and kidneys, using Hip-His-Leu as substrate. In a separate group, the heart was removed, the left ventricle (LV) was weighed and the LV/body weight ratios (LV/BW) were determined. We observed significant differences in MAP between the L-Arg-treated and untreated groups (129 +/- 7 vs. 168 +/- 6 mmHg; P< 0.01). The cardiac hypertrophy described for this model of hypertension was attenuated in the 2K1C-L-Arg-treated group (14th day, wet LV/BW: 2K1C-L-Arg = 1.88 +/- 0.1; 2K1C = 2.20 +/- 0.1 mg/g; P < 0.05). L-Arg administration caused an important decrease in cardiac ACE activity (2K1C-L-Arg: 118 +/- 15; 2K1C: 266 +/- 34 micromol/min/mg; P < 0.01). L-Arg also decreased the ACE activity in the clipped kidney by 47% (P < 0.01), but not in the nonclipped kidney. These data suggest that increased NO formation and reduced angiotensin II formation are involved in the anthihypertensive effect of orally administered L-arginine.
Brazilian Journal of Medical and Biological Research, 2007
We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in m... more We determined the effects of helium-neon (He-Ne) laser irradiation on wound healing dynamics in mice treated with steroidal and nonsteroidal anti-inflammatory agents. Male albino mice, 28-32 g, were randomized into 6 groups of 6 animals each: control (C), He-Ne laser (L), dexamethasone (D), D + L, celecoxib (X), and X + L. D and X were injected im at doses of 5 and 22 mg/kg, respectively, 24 h before the experiment. A 1-cm long surgical wound was made with a scalpel on the abdomens of the mice. Animals from groups L, D + L and X + L were exposed to 4 J (cm 2)-1 day-1 of He-Ne laser for 12 s and were sacrificed on days 1, 2, or 3 after the procedure, when skin samples were taken for histological examination. A significant increase of collagen synthesis was observed in group L compared with C (168 ± 20 vs 63 ± 8 mm 2). The basal cellularity values on day 1 were: C = 763
Brazilian Journal of Medical and Biological Research, 2011
Tamoxifen has been associated with a reduction in the incidence of myocardial infarction. However... more Tamoxifen has been associated with a reduction in the incidence of myocardial infarction. However, the effects of tamoxifen on coronary reactivity have not been fully elucidated. The objective of this study was to determine the effects of chronic treatment with tamoxifen on coronary vascular reactivity in spontaneously hypertensive rats (SHR). Female SHR were divided into four groups (N = 7 each): sham-operated (SHAM), sham-operated and treated with tamoxifen (0.1 mg/100 g) by gavage for 90 days (TAMOX), ovariectomized (OVX), and ovariectomized and treated with tamoxifen (OVX+TAMOX). Mean arterial pressure (MAP), heart rate (HR), coronary perfusion pressure (CPP), and coronary vascular reactivity were measured. MAP and HR were reduced (9.42 and 11.67%, respectively) in the OVX+TAMOX group compared to the OVX group (P < 0.01). The coronary vascular reactivity of the OVX+TAMOX group presented smaller vasoconstrictor responses to acetylcholine (2-64 µg) when compared to the OVX group (P < 0.01) and this response was similar to that of the SHAM group. The adenosine-induced vasodilator response was greater in the TAMOX group compared to the SHAM and OVX groups (P < 0.05). Baseline CPP was higher in OVX+TAMOX and TAMOX groups (136 ± 3.6 and 130 ± 1.5 mmHg) than in OVX and SHAM groups (96 ± 2 and 119 ± 2.3 mmHg; P < 0.01). Tamoxifen, when combined with OVX, attenuated the vasoconstriction induced by acetylcholine and increased the adenosine-induced vasodilatory response, as well as reducing the MAP, suggesting beneficial effects of tamoxifen therapy on coronary vascular reactivity after menopause.
Braz J Med Biol Res, 2004
Archives of Medical Research, 2008
Background. We investigated the influence of short-term treatment with supraphysiological doses o... more Background. We investigated the influence of short-term treatment with supraphysiological doses of an anabolic-androgenic steroid on the Bezold-Jarisch reflex (BJR) control of heart rate (HR) and whether this treatment induced cardiac hypertrophy and anabolic effects in rats. Methods. Male rats were treated with nandrolone decanoate (10 mg/kg À1 body weight/4 weeks; DECA) or vehicle control (CON). After 4 weeks of treatment, BJR was evaluated by bradycardia responses that were elicited by serotonin administration (2e32 mg/kg À1). Mean arterial pressure (MAP) was assessed and cardiac hypertrophy was determined by the left ventricle weight/body weight (LVW/BW) ratio. Histological analyses of LV and the measurement of the total body protein content of the animals were performed. Results. Nandrolone decanoate (ND) treatment had no effect on the MAP (CON 5 105 AE 5; DECA 5 110 AE 3 mmHg). However, the mean basal HR of DECA animals was significantly lower than that of control animals (CON 5 381 AE 14; DECA 5 324 AE 12 bpm; p !0.01). ND did not change the sensitivity of the BJR. The LVW/BW ratio indicated significant hypertrophy of the LV in DECA animals (CON 5 1.76 AE 0.04; DECA 5 2.0 AE 0.04 mg/g; p !0.01). Histological and morphometrical analyses demonstrate that there is also modest myocyte hypertrophy (CON 5 14.5 AE 1.5; DECA 5 20.0 AE 0.9 myocyte nuclei/field; p !0.05). However, the Masson-trichromic-stained samples showed an enhancement of collagen deposits on the LV matrix. Conclusions. We concluded that 4 weeks ND treatment induced an anabolic effect and the beginnings of LV remodeling, mainly due to excessive collagen deposition in the cardiac extracellular matrix. However, the treatment did not influence BJR control of bradycardia, an effect that could be explained by an enhanced efferent vagal tonus in DECA animals.
Acta Physiologica, 2010
The relaxation induced by oestrogen in the coronary vascular bed from normotensive rats has been ... more The relaxation induced by oestrogen in the coronary vascular bed from normotensive rats has been well described. However, almost nothing is known about this action in spontaneously hypertensive rats (SHR). We investigated the effect of 17 b-oestradiol (E 2) in coronary arteries from SHR as well as the contribution of the endothelium and the vascular smooth muscle to this action. Methods: Coronary arteries from male and female rats were used. Mean arterial pressure (MAP) and baseline coronary perfusion pressure (CPP) were determined. The effects of 10 lm E 2 were assessed by in bolus administration before and after endothelium denudation (0.25 lm sodium deoxycholate) or perfusion with 100 lm N x-nitro-l-arginine methyl ester (l-NAME), 2.8 lm indomethacin, 0.75 lm clotrimazole, 100 lm l-NAME after endothelium denudation (0.25 lm sodium deoxycholate), 100 lm l-NAME plus 2.8 lm indomethacin, 0.75 lm clotrimazole plus 2.8 lm indomethacin and 4 mm tetraethylammonium (TEA). Results: MAP was higher in the male group, while CPP was higher in the female group (P < 0.05). There were no differences in E 2-induced relaxation between females and males ()17 AE 1.6 vs.)17 AE 2% respectively). Only in the female group the E 2 response was significantly attenuated after endothelium removal or perfusion with clotrimazole. The response to E 2 was reduced in both groups with l-NAME, l-NAME plus indomethacin, l-NAME after endothelium removal or TEA. Conclusions: Nitric oxide, endothelium-derived hyperpolarizing factor and potassium channels may have the most important role to E 2 response in the female group, whereas nitric oxide and potassium channels may have the most important role in the male group.
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Papers by Margareth Ribeiro Moysés