Papers by Marcelle Danelon
Journal of Applied Oral Science
Effect of fluoride gels with nanosized sodium trimetaphosphate on the in vitro remineralization o... more Effect of fluoride gels with nanosized sodium trimetaphosphate on the in vitro remineralization of caries lesions* Objective: To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. Methodology: Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP), 4,500 ppm F (4500F), 4500F + 2.5% TMPnano (2.5% Nano), 4500F + 5% TMPnano (5% Nano), 4500F + 5% TMPmicro (5% Micro), 9,000 ppm F (9000F), and 12,300 ppm F (Acid gel). The gels were applied in a thin layer for one minute. Half of the blocks were subjected to pH cycling for six days, whereas the remaining specimens were used for loosely-(calcium fluoride; CaF 2) and firmly-bound (fluorapatite; FA) fluoride analysis. The percentage of surface hardness recovery (%SHR), area of subsurface lesion (ΔKHN), CaF 2 , FA, calcium (Ca), and phosphorus (P) on/in enamel were determined. Data (log 10-transformed) were subjected to ANOVA and the Student-Newman-Keuls' test (p<0.05). Results: We observed a dose-response relation between F concentrations in the gels without TMP for %SHR and ΔKHN. The 2.5% Nano and 5% Micro reached similar %SHR when compared with 9000F and Acid gels. For ΔKHN, Placebo and 5% Nano gels had the highest values, and 5% Micro, 2.5% Nano, 9000F, and Acid gels, the lowest. All groups had similar retained CaF 2 values, except for Placebo and Acid gel. We verified observed an increase in Ca concentrations in nano-sized TMP groups. Regarding P, TMP groups showed similar formation and retention to 9000F and Acid. Conclusion: Adding 2.5% nano-sized or 5% micrometric TMP to low-fluoride gels lead to enhanced in vitro remineralization of artificial caries lesions.
Brazilian Oral Research
The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated w... more The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (ΔKHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest ∆KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and ∆KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression.
Antibiotics
This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations c... more This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, and low concentrations of fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid and punicalagin). Total phenolics were quantified among formulations, and their stability was analyzed for 28 days. The formulation effects were evaluated as follows: (1) inorganic component concentration and reduced demineralization on bovine enamel blocks subjected to pH cycling; (2) anti-biofilm effect on dual-biofilms of Streptococcus mutans ATCC 25175 and Candida albicans ATCC 10231 treated for 1 and 10 min, respectively; and (3) cytotoxicity and production of inflammatory mediators (interleukin-6 and tumor necrosis factor-alpha). The formulation containing 3% PPE, 0.3% sodium-trimetaphosphate, and 225 ppm of fluoride resulted in a 34.5% surface hardness loss; a 13% (treated for 1 min...
Clinical Oral Investigations
OBJECTIVE This study evaluated in vitro the effects of calcium gluconate (CaGlu), sodium fluoride... more OBJECTIVE This study evaluated in vitro the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium hexametaphosphate (HMP), and NaF/TMP added to a 35% hydrogen peroxide (H2O2) bleaching gel on the color change, enamel hardness, and trans-amelodentinal diffusion. MATERIALS AND METHODS Enamel discs/bovine dentin (n = 150) were divided according to the bleaching gel: 35% H2O2 (H2O2); 35% H2O2 + 0.1% NaF (H2O2/NaF); 35% H2O2 + 1% HMP (H2O2/HMP); 35% H2O2 + 0.1% NaF + 1% HMP (H2O2/NaF/HMP), and 35% H2O2 + 2% CaGlu (H2O2/Caglu). The bleaching gels were applied three times (40 min/session) at 7-day intervals between each application. Then, color alteration (ΔE), whitening index (ΔWID), percentage of surface hardness loss (% SH), cross-sectional hardness (ΔKHN), and trans-amelodentinal diffusion were determined. Data were submitted for analysis of variance (ANOVA), followed by the Student-Newman-Keuls test (p < 0.05). RESULTS All bleaching gels showed significant color changes after treatment (p < 0.001). ΔE and ΔWID were similar among the evaluated gels. Mineral loss (% SH and ΔKHN) and trans-amelodentinal diffusion of hydrogen peroxide were lower for H2O2/NaF/HMP; the H2O2/CaGlu group presented the highest values about the other groups (p < 0.001). CONCLUSION It is possible to conclude that the addition of NaF/HMP to the in-office bleaching agent did not interfere with the bleaching efficacy and reduced enamel demineralization and H2O2 diffusion. CLINICAL SIGNIFICANCE The association of NaF/HMP to the bleaching gel can be used as a novel approach for minimizing the adverse effects of H2O2 by-products and with similar clinical efficacy.
Universidade Estadual Paulista (UNESP), Departamento de Ciencias Basicas, Faculdade de Odontologi... more Universidade Estadual Paulista (UNESP), Departamento de Ciencias Basicas, Faculdade de Odontologia de Aracatuba
Brazilian Oral Research, 2017
Archives of Health Investigation, 2016
Foi objetivo avaliar o efeito in vitro da adicao do trimetafosfato de sodio (TMP) microparticulad... more Foi objetivo avaliar o efeito in vitro da adicao do trimetafosfato de sodio (TMP) microparticulado (m) e nanoparticulado (n) no cimento de ionomero de vidro (CIV) sobre a desmineralizacao do esmalte. Blocos de esmalte (n=96) bovino foram selecionados pelo teste de dureza de superficie inicial (DS 1 ). Confeccionou-se 12 corpos-de-prova de cada material: CIV sem TMP (CIV); CIV com TMPm 3,5%, 7% e 14%; CIV com TMPn 3,5%, 7% e 14%. Como grupo controle foi utilizado 12 blocos sem material. Os corpos-de-prova dos CIVs foram adaptados aos blocos de esmalte e submetidos a ciclagem de pH. Analisou-se a dureza de superficie final (DS 2 ) e em seccao longitudinal (DSL) e a concentracao de fluoreto (F) no esmalte. Para as variaveis obtidas apos a ciclagem de pH (DS 2 , %DS, ΔKHN e F) foram considerados como fatores de variacao os materiais. Os resultados de DS 2 , %DS e ΔKHN foram submetidos a analise de variância, os dados de F foram submetidos a analise de Kruskal-Wallis, seguidos pelo teste...
Revista de Odontologia da UNESP, 2014
Universidade Estadual Paulista (UNESP), Faculdade de Odontologia (FOA), Departamento de Ciencias ... more Universidade Estadual Paulista (UNESP), Faculdade de Odontologia (FOA), Departamento de Ciencias Basicas, Aracatuba, SP, Brasil
Archives of Health Investigation, 2016
O presente estudo avaliou a eficacia de vernizes fluoretados suplementados ou nao com trimetafosf... more O presente estudo avaliou a eficacia de vernizes fluoretados suplementados ou nao com trimetafosfato (TMP) sobre a erosao e abrasao dentinaria. Discos de dentina bovina polidos (n = 60; diâmetro de 4 mm) foram selecionados por microdureza de superficie (SMH). Em seguida foram divididos em 5 grupos (n=12) de acordo com o tipo de verniz utilizado: sem F/TMP (Placebo), 5% NaF (5%NaF), 2,5% NaF (2,5%NaF), 2,5% NaF associado a 5% TMP (2,5NaF + 5%TMP), 5% NaF associado a 5% TMP (5%NaF + 5%TMP). O desafio erosivo foi promovido por imersao em acido citrico (0,05 M, pH 3,2) por 5 minutos (4 vezes ao dia) e o tratamento abrasivo, por escovacao mecânica por 15 segundos, por um periodo de 5 dias. Apos os desafios, determinou-se o desgaste dentinario (μm). Para analise dos dados foi considerado como fator de variacao o tipo de verniz experimental, e como variavel de medida, o desgaste da superficie dentinaria (µm). Os dados foram submetidos a analise de variância (1-criterio) seguida pelo teste ...
O declinio de carie dentaria nas ultimas decadas tem sido atribuido ao amplo uso de fluoreto em s... more O declinio de carie dentaria nas ultimas decadas tem sido atribuido ao amplo uso de fluoreto em suas diversas formas de apresentacao e aplicacao. A aplicacao do gel fluoretado visa maior formacao de fluoreto de calcio que se manteria em contato com o esmalte durante um periodo mais prolongado, mas sua alta concentracao de fluoreto traz tambem maior risco de intoxicacao aguda. O objetivo do presente estudo foi otimizar in vitro o potencial anticarie de geis com baixa concentracao de fluoreto suplementados com trimetafosfato de sodio (TMP). Selecionou-se 160 blocos de dentes bovinos atraves da dureza de superficie (SHi) sendo divididos em 8 grupos de geis (n=20): sem F e TMP (Placebo); TMP3%, TMP5%; 4500 µg F/g (4500); 4500 µg F/g + TMP3% (4500 TMP3%), 4500 µg F/g + TMP5% (4500 TMP5%), 9000 µg F/g (9000) e 12300 µg F/g (Gel acido). Os blocos foram tratados uma unica vez (1 min.) com os geis, antes de serem submetidos a ciclagem de pH durante cinco dias. A seguir, determinou-se a durez...
Archives of Health Investigation, 2016
O objetivo foi avaliar a incorporacao do hexametafosfato de sodio (HMP) microparticulado (m) e na... more O objetivo foi avaliar a incorporacao do hexametafosfato de sodio (HMP) microparticulado (m) e nanoparticulado (n) no cimento de ionomero de vidro na liberacao de fluoreto (F) e sobre a desmineralizacao do esmalte. Foram confeccionados 6 corpos-de-prova (c-d-p) para os grupos: CIV, CIV com 6, 9 e 12% de HMPm, CIV com 6, 9 e 12% de HMPn. Os c-d-p foram submetidos a ciclagem de pH durante 15 dias. As solucoes da ciclagem foram recolhidas diariamente para analise de F liberado. Blocos de esmalte (n=80) bovino foram selecionados pelo teste de dureza de superficie inicial (DS 1 ) adaptados a c-d-p dos diferentes grupos dos CIVs e imersos diariamente em solucao de desmineralizacao (6h) e remineralizacao (18h) durante 7 dias. Ao final da ciclagem realizou-se dureza de superficie final (DS 2 ) para o calculo da % de perda de dureza (%PD). Os dados de liberacao de fluor foram submetidos a analise de variância e os de %PD ao teste de Kruskal-Wallis, seguido pelo teste de Student-Newman-Keuls ...
Archives of Health Investigation, 2016
O objetivo do presente estudo foi avaliar a capacidade de um gel topico de pH neutro, com concent... more O objetivo do presente estudo foi avaliar a capacidade de um gel topico de pH neutro, com concentracao reduzida de fluoreto (F) e suplementado com trimetafosfato de sodio (TMP), em reduzir in situ a desmineralizacao do esmalte dentario. Este estudo foi cego e cruzado, realizado em 5 fases experimentais com duracao de 7 dias cada. Voluntarios (n=12) utilizaram dispositivos palatinos, contendo inicialmente 4 blocos de esmalte selecionados pela dureza de superficie inicial (SHi). O desafio cariogenico foi realizado com sacarose a 30% (6×/dia). Os regimes de tratamentos foram: Gel + Dentifricio sem F (Gel Placebo); Dentifricio com 1100 µg F/g (DF); Gel com 4500 µg F/g suplementado com 5%TMP (Gel TMP), Gel com 9000 µg F/g (Gel 9000) e Gel acido (Gel acido). Imediatamente apos a aplicacao topica do gel ou escovacao com DP/DF, por 1 minuto, 2 blocos foram removidos para a analise da concentracao de fluoreto de calcio formado no esmalte (CaF 2 ) (n=120). Apos o periodo experimental (7 dias)...
Archives of Health Investigation, 2016
Varios materiais sao utilizados em dentifricios com finalidade de depositar o material no interio... more Varios materiais sao utilizados em dentifricios com finalidade de depositar o material no interior dos canaliculos ou obstruir fisicamente as extremidades abertas dos tubulos dentinarios (TD) para aliviar a dor dentinaria. O trimetafosfato de sodio (TMP) e um sal de fosfato inorgânico que apresenta capacidade de interagir com a hidroxiapatita. O objetivo foi avaliar in vitro a acao de dentifricios suplementados com TMP microparticulado (m) e nanoparticulado (n), associado ao fluoreto (F) na obliteracao dos TD apos desafio acido. Blocos de dentina bovina (4x4x2mm) tiveram os TD desobliterados. Os blocos (n=30) foram submetidos a escovacao mecânica 2x/dia por 7 dias com dentifricios: placebo (sem F e sem TMP), 1100 ppm F sem TMP, 1100 ppm F associado a 3% TMPm, 1100 ppm F associado a 3% TMPn. Os blocos foram imersos em acido citrico (pH 3,2) por 1 minuto, sob agitacao. Dez blocos nao foram submetidos a tratamento. Analisou-se a superficie dentinaria quanto a area, o diâmetro e numero ...
Revista de Odontologia da UNESP, 2012
European Archives of Paediatric Dentistry, 2021
PURPOSE To evaluate the type and the amount of fluoridated dentifrice applied on children's t... more PURPOSE To evaluate the type and the amount of fluoridated dentifrice applied on children's toothbrushes by parents/guardians according to descriptions typically recommended by scientific societies, as well as to assess the influence of demographic and socioeconomic variables on dentifrice use. METHODS Parents/guardians of children (0-7 years old; n = 306; convenience sample) attending vaccination centres from Araçatuba (Brazil), answered to a structured questionnaire comprising items related to interviewees' education, child's age, gender, brushing habits and use of fluoridated dentifrice. The amount of toothpaste used by children during toothbrushing was estimated using a portable scale. Similarly, the interviewees were requested to apply dentifrices on toothbrushes according to eight descriptions, ranging from "smear" to "all bristles", following a random sequence. Data were submitted to Mann-Whitney's, Kruskal-Wallis' and Friedman's tests, and Spearman's correlation coefficient (p < 0.05). RESULTS The type of toothpaste and the amount of product used at home were not affected by the respondents' educational level or family income. However, child's age was significantly correlated with the amount of toothpaste placed on the toothbrush (r = 0.324, p < 0.001). Also, the amount of toothpaste placed on the toothbrush increased according to what would be expected from the descriptions, although wide variations were observed within each description, with large interquartile and overall ranges. CONCLUSION The amount and the type of dentifrice used by children were influenced by their age, while parents/caregivers' interpretation on verbal instructions regarding appropriate dentifrice quantities varied widely. This reinforces the need for educative measures on the appropriate use by dentifrices by children.
Journal of Dentistry, 2019
To evaluate the antimicrobial/antibiofilm and mechanical properties, and the effect on enamel dem... more To evaluate the antimicrobial/antibiofilm and mechanical properties, and the effect on enamel demineralization of a resin-modified GIC (RMGIC) containing CHX and nano-sized sodium trimetaphosphate (TMP). Methods: RMGIC was associated with CHX (1.25 or 2.5%) and/or TMP (7 or 14%). Antimicrobial and antibiofilm activity were assessed using agar diffusion test and evaluation of biofilm metabolism, respectively. In addition, fluoride (F) and TMP releases as well as the diametral tensile (DTS) and compressive (CS) strength were determined. The percentage of mineral loss (%SH), integrated loss of subsurface hardness (ΔKHN) and enamel F concentrations were also evaluated. Results: RMGICs containing CHX associated or not with TMP presented higher inhibition zones and effect on S. mutans biofilm. A reduction on CS was observed only for RMGIC + 2.5%CHX and on DTS for RMGIC + 2.5%CHX + 14%TMP. The highest F and TMP releases and lowest %SH and ΔKHN values were detected for RMGIC + 1.25%CHX + 14%TMP and RMGIC + 2.5%CHX + 14%TMP. Higher enamel F concentrations were observed for TMP groups. Conclusion: 1.25%CHX and 14%TMP increased antimicrobial/antibiofilm action and the ability to prevent enamel demineralization, with minimal effect on the mechanical properties of RMGIC. Clinical significance: RMGIC containing CHX and TMP is an alternative material for patients at high risk for dental caries and can be indicated for low-stress regions or provisional restorations.
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Papers by Marcelle Danelon