Papers by María Carolina Amezcua Vesely
International Immunology, Mar 5, 2010
Cell Research, Jan 5, 2018
Journal of Molecular Biology, 2021
PLOS Neglected Tropical Diseases, May 4, 2010
Frontiers in Immunology
This work examines cellular immunity against SARS-CoV-2 in patients from Córdoba, Argentina, duri... more This work examines cellular immunity against SARS-CoV-2 in patients from Córdoba, Argentina, during two major waves characterized by different circulating viral variants and different social behavior. Using flow cytometry, we evaluated the main lymphocyte populations of peripheral blood from hospitalized patients with moderate and severe COVID-19 disease. Our results show disturbances in the cellular immune compartment, as previously reported in different cohorts worldwide. We observed an increased frequency of B cells and a significant decrease in the frequency of CD3+ T cells in COVID-19 patients compared to healthy donors (HD). We also found a reduction in Tregs, which was more pronounced in severe patients. During the first wave, the frequency of GZMB, CD107a, CD39, and PD-1-expressing conventional CD4+ T (T conv) cells was significantly higher in moderate and severe patients than in HD. During the second wave, only the GZMB+ T conv cells of moderate and severe patients increase...
Journal of Experimental Medicine, Jun 11, 2020
Cell, Aug 1, 2019
P. made key suggestions for experiments and edited the manuscript. P.B. performed single cell exp... more P. made key suggestions for experiments and edited the manuscript. P.B. performed single cell experiment and M.S.K. provided key suggestion for the single cell experiment. C.C.D.H, J.Z. Y.K. and L.K. performed RNA-seq and single cell analysis. A.I and N.I. helps with parabiosis experiments. D.H.K. provided Candida albicans. M.C.A.V. did parabiosis and performed all the others experiments.
BENTHAM SCIENCE PUBLISHERS eBooks, Mar 31, 2012
Frontiers in Immunology
BackgroundCOVID-19 severity has been linked to an increased production of inflammatory mediators ... more BackgroundCOVID-19 severity has been linked to an increased production of inflammatory mediators called “cytokine storm”. Available data is mainly restricted to the first international outbreak and reports highly variable results. This study compares demographic and clinical features of patients with COVID-19 from Córdoba, Argentina, during the first two waves of the pandemic and analyzes association between comorbidities and disease outcome with the “cytokine storm”, offering added value to the field.MethodsWe investigated serum concentration of thirteen soluble mediators, including cytokines and chemokines, in hospitalized patients with moderate and severe COVID-19, without previous rheumatic and autoimmune diseases, from the central region of Argentina during the first and second infection waves. Samples from healthy controls were also assayed. Clinical and biochemical parameters were collected.ResultsComparison between the two first COVID-19 waves in Argentina highlighted that p...
<p>A) Plasma IFN-γ and TNF concentration in 20-day <i>T. cruzi</i> infected WT ... more <p>A) Plasma IFN-γ and TNF concentration in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice. Data are shown as mean ± SD, n = 6 mice per group. P values calculated using two-tailed T test. B) Plasma IFN-γ concentration in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-IFN-γ. P values calculated using two-tailed T test. C) Parasitemia determined in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-IFN-γ. Data are shown as mean ± SD, n = 6 mice per group. P values calculated using two-tailed T test. D) Activity of ALT and AST determined in the plasma of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-IFN-γ. Data are shown as mean ± SD, n = 6 mice per group. P values calculated using two-tailed T test. E) Survival of <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-IFN-γ. P value calculated with a Gehan-Breslow-Wilcoxon test, n = 12 per group. Data in A and in B–E are representative of four and two independent experiments, respectively.</p
a<p>Scale: +: mild; ++:moderate; +++: severe; ++++: extremely severe.</p>b<p>PM... more a<p>Scale: +: mild; ++:moderate; +++: severe; ++++: extremely severe.</p>b<p>PMN: Polymorphonuclear cells; Mo/Ly: monocyte/lymphocytes.</p
<p>A) IL-17A and IL-17F concentration determined in the culture supernatants of spleen and ... more <p>A) IL-17A and IL-17F concentration determined in the culture supernatants of spleen and lymph node cells obtained from mice at different times post <i>T. cruzi</i> infection and stimulated during 48 h in the presence of CD3/PdBU. Data are shown as mean ± SD, n = 5 mice per group. B) Plasma IL-17A concentration in non-infected (NI) or 20-day <i>T. cruzi</i>-infected (I) mice. Data are shown as mean ± SD, n = 5 mice per group. C) Percentage of IL-17A positive cells after 5 h PMA/Ionomycin stimulation of liver cell suspensions obtained from 20-day <i>T. cruzi</i>-infected mice. Plot representative of one out of five mice. D) Percentage of IL-17A positive cells within CD3 positive and CD3 negative cell population after 5 h PMA/Ionomycin stimulation of spleen, lymph nodes and liver cells from 20-day <i>T. cruzi</i>-infected mice. Data are shown as mean ± SEM, n = 5 mice per group. E) IL-17A concentration determined in the culture supernatants of NK1.1+ cells, CD4+ and CD8+ T cells, Gr-1+ cells and the remaining negative fraction (NF) sorted from the spleen and liver of 20-day <i>T. cruzi</i> infected mice and stimulated during 48 h. Data are shown as mean ± SD of triplicates cultures. F) Plasma IL-17E concentration in non-infected (NI) or 20-day <i>T. cruzi</i>-infected (I) mice. Data are shown as mean ± SD, n = 5 mice per group. Data in A, E and F and in B–D are representative of two and three independent experiments, respectively.</p
<p>A) CD11b+Gr-1+ frequency and absolute numbers in blood, spleen and liver of 20-day <i... more <p>A) CD11b+Gr-1+ frequency and absolute numbers in blood, spleen and liver of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly6G mAbs. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated by two-tailed T test. B) Concentration of IL-10 determined in 48 h unstimulated culture supernatants of spleen and liver cell suspensions obtained from 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G mAbs. Each symbol represents a different mouse and horizontal line indicates the mean. P values calculated with two-tailed T test. C) Plasma IFN-γ and TNF concentration in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G mAbs. Each symbol represents a different mouse and horizontal line indicates the mean. P values calculated with two-tailed T test. D) Activity of ALT and AST determined in the plasma of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated using two-tailed T test. E) Parasitemia determined in 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G Abs. Data are shown as mean ± SD, n = 6–8 mice per group. P values calculated using two-tailed T test. F) Survival of <i>T. cruzi</i> infected WT and IL-17RA KO mice treated with anti-Ly-6G. P value calculated with a Gehan-Breslow-Wilcoxon test, n = 12 per group. Data in A–D and in E are representative of three and two independent experiments, respectively.</p
<p>A) Parasitemia determined at day 20 post-infection in infected WT and IL-17RA KO mice ad... more <p>A) Parasitemia determined at day 20 post-infection in infected WT and IL-17RA KO mice adoptively transferred with WT neutrophils. Data are shown as mean ± SD, n = 6 per group. P values calculated with two-tailed T test. B) Concentration of IFN-γ in plasma of 20-day <i>T. cruzi</i> infected WT and IL-17R KO mice adoptively transferred with WT neutrophils. Data are shown as mean ± SD, n = 6 per group. P values calculated with two-tailed T test. C) Survival of <i>T. cruzi</i> infected WT and IL-17R KO mice adoptively transferred with WT neutrophils. P value calculated with a Gehan-Breslow-Wilcoxon test, n = 12 per group. D) Parasitemia determined at day 20 postinfection in infected WT mice adoptively transferred with WT and IL-10 KO neutrophils. Data are shown as mean ± SD, n = 12 per group. P values calculated with two-tailed T test. E) Concentration of IFN-γ in plasma of 20-day <i>T. cruzi</i> infected WT mice adoptively transferred with WT and IL-10 KO neutrophils. Data are shown as mean ± SD, n = 12 per group. P values calculated with two-tailed T test. F) Survival of <i>T. cruzi</i> infected WT mice adoptively transferred with WT and IL-10 KO neutrophils. P value calculated with a Gehan-Breslow-Wilcoxon test, n = 12 per group. Data in A–F are representative of two independent experiments.</p
<p>A–B) Concentration of IL-10 and TNF determined in 48 h culture supernatants of Ly-6G+ ne... more <p>A–B) Concentration of IL-10 and TNF determined in 48 h culture supernatants of Ly-6G+ neutrophils purified from bone marrow of WT and IL-17RA KO mice (A) and of CD11b+Ly-6G+ neutrophils sorted from spleen 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice (B) and stimulated as indicated. Data are shown as mean ± SD of cuatriplicates. C) Proliferation and percentage of IFN-γ-producing CD3 positive splenocytes from normal mice after 5 day stimulation in anti-CD3 and anti-CD28 coated plates in the presence of CD11b+Ly-6G+ neutrophils obtained from 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice and of a blocking anti-IL10R Ab. Data are shown as mean ± SD of triplicates. P values calculated using two-tailed T test. Data in A–C are representative of two independent experiments.</p
<p>A) Cell numbers in spleen, lymph nodes and liver of WT and IL-17RA KO mice determined at... more <p>A) Cell numbers in spleen, lymph nodes and liver of WT and IL-17RA KO mice determined at different times after <i>T. cruzi</i> infection. Data are shown as mean ± SD, n = 5 mice per group. P values calculated using two-way ANOVA followed by Bonferroni's posttest. B) Percentage of CD11b+ Gr-1+ neutrophils in spleen and liver of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice. Plots are representative one out of five mice. C) Absolute numbers of CD11b+ Gr-1+ neutrophils in spleen (left) and liver (right) of 20-day <i>T. cruzi</i> infected WT and IL-17RA KO mice. Each symbol represents a different mouse and horizontal line indicates the mean. P values calculated with two-tailed T test. D) Concentration of CXCL1, CXCL2 and CXCL10 in spleen and liver homogenates obtained from WT and IL-17RA KO mice at different times post <i>T. cruzi</i> infection. Data are shown as mean ± SD of biological triplicates, normalized to total protein concentration, n = 5 mice per group. P values calculated with two-way ANOVA followed by Bonferroni's posttest. (* p: spleen WT vs spleen KO; # p: liver WT vs liver KO). E) Absolute number or frequency of transferred WT and IL-17RA KO neutrophils detected in bone marrow, blood, spleen and liver of non-infected (NI) and infected (I) WT and IL-17RA KO mice 3 h after i.v. injection. Data are shown as mean ± SD, n = 5 per group. P values calculated with two-tailed T test. Data in A–C and D–E are representative of four and two independent experiments, respectively.</p
a<p>Scale: +: mild; ++:moderate; +++: severe; ++++: extremely severe.</p>b<p>PM... more a<p>Scale: +: mild; ++:moderate; +++: severe; ++++: extremely severe.</p>b<p>PMN: Polymorphonuclear cells; Mo/Ly: monocyte/lymphocytes.</p
Nature Communications, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the pa... more An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Papers by María Carolina Amezcua Vesely