Aims-The labelling index as defined by the percentage of bone marrow plasma cells doubling their ... more Aims-The labelling index as defined by the percentage of bone marrow plasma cells doubling their DNA in the S phase is a useful prognostic factor in multiple myeloma. The aim of this study was to examine the specificity and sensitivity of a new flow cytometric method for measuring the labelling index. Methods-Bone marrow specimens from five patients with monoclonal gammopathy of undetermined significance and 61 patients with multiple myeloma were investigated. The labelling index (LI%) was determined by means of a microscopic and flow cytometric method using the antibromodeoxyuridine antibody BU-1. Serum thymidine kinase, another index ofproliferation, was measured by radioimmunoassay.
American Journal of Clinical Pathology, Jul 1, 1996
The relationship between two cellular prognostic parameters of multiple myeloma, the plasma cell ... more The relationship between two cellular prognostic parameters of multiple myeloma, the plasma cell labeling index (LI%) and bone marrow histology was studied. The LI% as the percentage of monoclonal plasma cells in the S-phase was measured by bromodeoxyuridine incorporation using the anti-bromodeoxyuridine antibody BU-1. The histologic classification was based on six plasma cell types that allow prognostic grading as the Marschalko, small cell, cleaved, polymorphous, asynchronous, and blastic types. The biopsies also were used for estimating the degree of infiltration, /^-microglobulin (IMx assay, Abbott, North Chicago, IL), the most significant serum parameter for myeloma also was measured for comparison. Bone marrow specimens and sera were obtained from 50 myeloma patients. Fourteen patients with smoldering myeloma were characterized by well-differentiated Marschalko or small cell type cells, a low LI%, and a low /^-microglobulin concentration. Considering all myeloma patients, plasma cell type and degree of infiltration showed a significant correla-From the
Mllnchcn. Kliniturn Grosshadcrn. M luchioninis1r:I~sc 15. 0-8000 München 70. Federal Republic of ... more Mllnchcn. Kliniturn Grosshadcrn. M luchioninis1r:I~sc 15. 0-8000 München 70. Federal Republic of Gcrm3ny l lnstit ut ruf Klinische Chemie. JMedi:zinische Poliklinik, ' Dermatologische Klinik. ' Augenklinik Harlachin g. 6P.ychiatriI.Che Klinik. Ludwig-Maximilians-Universi' lI1. 0 •8000 München. redenl Republic of G ermany Summary. Thc major pmhological abnormalilic~ of HI V cnccphalopalhy lITe infiltrates of m:lcrophagcs. multinuc1eatcd giant cells. mieroglial nodulcs and dc myclination. Elevil!ed myelin basic pralein (MBP) levels in {he cerebrospinal fluid (CS F) providc a marker for cenual nervous system demyeli• nation. The purpose of this study was 10 invesligale the possible role of CSF MBP as ,I uscful and early markcr for HI V cnccphalopathy. The CS F of 40 consccutive paticms with HIV infcction of various dinieal stages was invcstigated. induding 13 patienls with dinical signs of 1"lI V eneephalopathy. CSF MB P was clcvatcd in 2 paticms (5 .0 and 5.3nglml). bolh of whorn hOld moderate 10 severe HIV encephalopathy. Thc course ofl he disease was rapid in both patients. In the remaining 38 paticms. CSF MBP levels werc marginally elcvaled (11 = 12) or no rmal (11 "" 26)_ Our results suggest that CSF MBP is not iI sensitive marker for the diagnosis and evaluation of HIV encephalopathy. but may be an indieator of prognosis for Ihe course of the discase. Therc wcre only fcw findings of clcvatcd CSF MBP levels in patiems with HI V encephalapath y in the CUTrent study. and Ihis may be because the disorder progres.<;ed slowly in most patients. It is possible thaI CSF MB P levels in HI V cneephalopalhy mny only be elevated with ae ute cl inieal deterioration bUl are normal in slowly progressive form s of dern yelin ation. as seen in multiple sclerosis.
Introduction Cerebrospinal fluid (CSF) analysis is important for detecting inflammation of the ne... more Introduction Cerebrospinal fluid (CSF) analysis is important for detecting inflammation of the nervous system and the meninges, bleeding in the area of the subarachnoid space that may not be visualized by imaging, and the spread of malignant diseases to the CSF space. In the diagnosis and differential diagnosis of neurodegenerative diseases, the importance of CSF analysis is increasing. Measuring the opening pressure of CSF in idiopathic intracranial hypertension and at spinal tap in normal pressure hydrocephalus constitute diagnostic examination procedures with therapeutic benefits. Recommendations (most important 3-5 recommendations on a glimpse): The indications and contraindications must be checked before lumbar puncture (LP) is performed, and sampling CSF requires the consent of the patient.Puncture with an atraumatic needle is associated with a lower incidence of postpuncture discomfort. The frequency of postpuncture syndrome correlates inversely with age and body mass index, ...
Bakterielle ZNS-Erkrankungen bei systemischen Infektionen, 1998
In der orientierenden Notfalldiagnostik last sich die Schrankenfunktion durch die Bestimmung des ... more In der orientierenden Notfalldiagnostik last sich die Schrankenfunktion durch die Bestimmung des Gesamteiweises im Liquor abschatzen. Eine prazisere Aussage ist jedoch nur durch die Ermittelung des Liquorserum-Albuminquotienten mit immunchemischen Methoden moglich, da Albumin als Einzelprotein mit relativ hoher Konzentration einerseits im Liquor gut mesbar ist und andererseits mit Sicherheit unter keinen Umstanden im ZNS produziert wird. Somit gibt der Liquorserumkonzentrationsgradient fur Albumin zumindest die Schrankenpermeabilitat fur Proteine der Grose von ca. 70 k Dalton korrekt wieder, und das Ergebnis wird nicht durch lokal freigesetzte Proteine verfalscht.
Verhandlungen der Deutschen Gesellschaft für Neurologie, 1987
High-dose 7S-intravenous immunoglobulins (7S i.v.IG) have been demon strated to improve acute aut... more High-dose 7S-intravenous immunoglobulins (7S i.v.IG) have been demon strated to improve acute autoimmune idiopathic thrombocytopenic purpura (ITP) in children (1).
Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verz... more Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verzeichnet diese Publikation in der Deutschen Nationalbibliografie; detaillierte bibliografische Daten sind im Internet über http://dnb.d-nb.de abrufbar.
... Or filter your current search. Brunel D, Find all citations by this author (default). Or filt... more ... Or filter your current search. Brunel D, Find all citations by this author (default). Or filter your current search. de Rohan-Chabot P, Find all citations by this author (default). Or filter your current search. Raphael JC, Find all citations by this author (default). ...
Autoimmune phenomena are of major importance in the pathogenesis of the main paraneoplastic disea... more Autoimmune phenomena are of major importance in the pathogenesis of the main paraneoplastic diseases in humans. The autoimmune process is probably triggered by oncogenic antigens, which are cross-reactive or identical with certain neural antigens. Despite the known pathomechanism these syndromes are still considered as non-treatable. We report two cases of paraneoplastic cerebellar degeneration, in which intensive immunosuppression with cytostatics lead to considerable neurological improvement.
Zur Diagnosesicherung der Myasthenia gravis dient die Bestimmung von Autoantikorpern gegen den ni... more Zur Diagnosesicherung der Myasthenia gravis dient die Bestimmung von Autoantikorpern gegen den nikotinischen Acetylcholin-Rezeptor (AChR) des Muskels (Ubersicht bei 6). Dies erfolgt mit Hilfe eines Immunprazipitationstests (1,2). Als Antigen-Quelle wird menschliches Material verwendet (3), hauptsachlich Amputate von Patienten mit Diabetes mellitus. Die Amputate sind oft nur schwer verfugbar und im Hinblick auf die Menge und Art des AChR nicht standardisiert. Bei der partiellen Denervierung durch die diabetische Polyneuropathie steigt der Anteil an extrajunktionalen AChR, der sich auch immunologisch von dem junktionalen Rezeptor der Synapse unterscheidet. Dadurch sind die anti-AChR-Antikorper- Werte zwischen unterschiedlichen Amputatpraparationen nicht exakt vergleichbar.
Aims-The labelling index as defined by the percentage of bone marrow plasma cells doubling their ... more Aims-The labelling index as defined by the percentage of bone marrow plasma cells doubling their DNA in the S phase is a useful prognostic factor in multiple myeloma. The aim of this study was to examine the specificity and sensitivity of a new flow cytometric method for measuring the labelling index. Methods-Bone marrow specimens from five patients with monoclonal gammopathy of undetermined significance and 61 patients with multiple myeloma were investigated. The labelling index (LI%) was determined by means of a microscopic and flow cytometric method using the antibromodeoxyuridine antibody BU-1. Serum thymidine kinase, another index ofproliferation, was measured by radioimmunoassay.
American Journal of Clinical Pathology, Jul 1, 1996
The relationship between two cellular prognostic parameters of multiple myeloma, the plasma cell ... more The relationship between two cellular prognostic parameters of multiple myeloma, the plasma cell labeling index (LI%) and bone marrow histology was studied. The LI% as the percentage of monoclonal plasma cells in the S-phase was measured by bromodeoxyuridine incorporation using the anti-bromodeoxyuridine antibody BU-1. The histologic classification was based on six plasma cell types that allow prognostic grading as the Marschalko, small cell, cleaved, polymorphous, asynchronous, and blastic types. The biopsies also were used for estimating the degree of infiltration, /^-microglobulin (IMx assay, Abbott, North Chicago, IL), the most significant serum parameter for myeloma also was measured for comparison. Bone marrow specimens and sera were obtained from 50 myeloma patients. Fourteen patients with smoldering myeloma were characterized by well-differentiated Marschalko or small cell type cells, a low LI%, and a low /^-microglobulin concentration. Considering all myeloma patients, plasma cell type and degree of infiltration showed a significant correla-From the
Mllnchcn. Kliniturn Grosshadcrn. M luchioninis1r:I~sc 15. 0-8000 München 70. Federal Republic of ... more Mllnchcn. Kliniturn Grosshadcrn. M luchioninis1r:I~sc 15. 0-8000 München 70. Federal Republic of Gcrm3ny l lnstit ut ruf Klinische Chemie. JMedi:zinische Poliklinik, ' Dermatologische Klinik. ' Augenklinik Harlachin g. 6P.ychiatriI.Che Klinik. Ludwig-Maximilians-Universi' lI1. 0 •8000 München. redenl Republic of G ermany Summary. Thc major pmhological abnormalilic~ of HI V cnccphalopalhy lITe infiltrates of m:lcrophagcs. multinuc1eatcd giant cells. mieroglial nodulcs and dc myclination. Elevil!ed myelin basic pralein (MBP) levels in {he cerebrospinal fluid (CS F) providc a marker for cenual nervous system demyeli• nation. The purpose of this study was 10 invesligale the possible role of CSF MBP as ,I uscful and early markcr for HI V cnccphalopathy. The CS F of 40 consccutive paticms with HIV infcction of various dinieal stages was invcstigated. induding 13 patienls with dinical signs of 1"lI V eneephalopathy. CSF MB P was clcvatcd in 2 paticms (5 .0 and 5.3nglml). bolh of whorn hOld moderate 10 severe HIV encephalopathy. Thc course ofl he disease was rapid in both patients. In the remaining 38 paticms. CSF MBP levels werc marginally elcvaled (11 = 12) or no rmal (11 "" 26)_ Our results suggest that CSF MBP is not iI sensitive marker for the diagnosis and evaluation of HIV encephalopathy. but may be an indieator of prognosis for Ihe course of the discase. Therc wcre only fcw findings of clcvatcd CSF MBP levels in patiems with HI V encephalapath y in the CUTrent study. and Ihis may be because the disorder progres.<;ed slowly in most patients. It is possible thaI CSF MB P levels in HI V cneephalopalhy mny only be elevated with ae ute cl inieal deterioration bUl are normal in slowly progressive form s of dern yelin ation. as seen in multiple sclerosis.
Introduction Cerebrospinal fluid (CSF) analysis is important for detecting inflammation of the ne... more Introduction Cerebrospinal fluid (CSF) analysis is important for detecting inflammation of the nervous system and the meninges, bleeding in the area of the subarachnoid space that may not be visualized by imaging, and the spread of malignant diseases to the CSF space. In the diagnosis and differential diagnosis of neurodegenerative diseases, the importance of CSF analysis is increasing. Measuring the opening pressure of CSF in idiopathic intracranial hypertension and at spinal tap in normal pressure hydrocephalus constitute diagnostic examination procedures with therapeutic benefits. Recommendations (most important 3-5 recommendations on a glimpse): The indications and contraindications must be checked before lumbar puncture (LP) is performed, and sampling CSF requires the consent of the patient.Puncture with an atraumatic needle is associated with a lower incidence of postpuncture discomfort. The frequency of postpuncture syndrome correlates inversely with age and body mass index, ...
Bakterielle ZNS-Erkrankungen bei systemischen Infektionen, 1998
In der orientierenden Notfalldiagnostik last sich die Schrankenfunktion durch die Bestimmung des ... more In der orientierenden Notfalldiagnostik last sich die Schrankenfunktion durch die Bestimmung des Gesamteiweises im Liquor abschatzen. Eine prazisere Aussage ist jedoch nur durch die Ermittelung des Liquorserum-Albuminquotienten mit immunchemischen Methoden moglich, da Albumin als Einzelprotein mit relativ hoher Konzentration einerseits im Liquor gut mesbar ist und andererseits mit Sicherheit unter keinen Umstanden im ZNS produziert wird. Somit gibt der Liquorserumkonzentrationsgradient fur Albumin zumindest die Schrankenpermeabilitat fur Proteine der Grose von ca. 70 k Dalton korrekt wieder, und das Ergebnis wird nicht durch lokal freigesetzte Proteine verfalscht.
Verhandlungen der Deutschen Gesellschaft für Neurologie, 1987
High-dose 7S-intravenous immunoglobulins (7S i.v.IG) have been demon strated to improve acute aut... more High-dose 7S-intravenous immunoglobulins (7S i.v.IG) have been demon strated to improve acute autoimmune idiopathic thrombocytopenic purpura (ITP) in children (1).
Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verz... more Bibliografische Information der Deutschen Nationalbibliothek Die Deutsche Nationalbibliothek verzeichnet diese Publikation in der Deutschen Nationalbibliografie; detaillierte bibliografische Daten sind im Internet über http://dnb.d-nb.de abrufbar.
... Or filter your current search. Brunel D, Find all citations by this author (default). Or filt... more ... Or filter your current search. Brunel D, Find all citations by this author (default). Or filter your current search. de Rohan-Chabot P, Find all citations by this author (default). Or filter your current search. Raphael JC, Find all citations by this author (default). ...
Autoimmune phenomena are of major importance in the pathogenesis of the main paraneoplastic disea... more Autoimmune phenomena are of major importance in the pathogenesis of the main paraneoplastic diseases in humans. The autoimmune process is probably triggered by oncogenic antigens, which are cross-reactive or identical with certain neural antigens. Despite the known pathomechanism these syndromes are still considered as non-treatable. We report two cases of paraneoplastic cerebellar degeneration, in which intensive immunosuppression with cytostatics lead to considerable neurological improvement.
Zur Diagnosesicherung der Myasthenia gravis dient die Bestimmung von Autoantikorpern gegen den ni... more Zur Diagnosesicherung der Myasthenia gravis dient die Bestimmung von Autoantikorpern gegen den nikotinischen Acetylcholin-Rezeptor (AChR) des Muskels (Ubersicht bei 6). Dies erfolgt mit Hilfe eines Immunprazipitationstests (1,2). Als Antigen-Quelle wird menschliches Material verwendet (3), hauptsachlich Amputate von Patienten mit Diabetes mellitus. Die Amputate sind oft nur schwer verfugbar und im Hinblick auf die Menge und Art des AChR nicht standardisiert. Bei der partiellen Denervierung durch die diabetische Polyneuropathie steigt der Anteil an extrajunktionalen AChR, der sich auch immunologisch von dem junktionalen Rezeptor der Synapse unterscheidet. Dadurch sind die anti-AChR-Antikorper- Werte zwischen unterschiedlichen Amputatpraparationen nicht exakt vergleichbar.
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