Journal of Pediatric Hematology Oncology, Aug 1, 2012
We report a 2.5-year-old boy with an X-linked lymphoproliferative disease (XLP) phenotype who pre... more We report a 2.5-year-old boy with an X-linked lymphoproliferative disease (XLP) phenotype who presented with human herpes virus-8 (HHV-8)-related hemophagocytic lymphohistiocytosis (HLH). XLP is a rare primary immunodeficiency characterized by extreme susceptibility to herpes viruses, mainly Epstein-Barr virus (EBV). Approximately 60% of patients with XLP present with fulminant mononucleosis associated with HLH, whereas remaining patients present with hypogammaglobulinemia or lymphoproliferative disease. Most commonly, one of the XLP phenotypes appears after exposure to EBV, but at least 12% of affected individuals developed symptoms without an evidence of EBV infection. Rarely, patients with XLP may present with central nervous system vasculitis or aplastic anemia. HHV-8 is lymphotrophic and it is associated with lymphoproliferative disorders and Kaposi sarcoma in immunodeficient hosts. Kaposi sarcoma rarely occurs in children with well-defined primary immunodeficiency. Also, HHV-8-related HLH was previously reported in 2 siblings with a perforin gene deficiency. Recently, it became evident that besides EBV, other viruses may trigger the symptoms in XLP. We report for the first time HHV-8-related HLH in EBV-negative pediatric patient with an XLP phenotype.
Since emerging from Wuhan, China, in December of 2019, the novel coronavirus named SARS-CoV-2 has... more Since emerging from Wuhan, China, in December of 2019, the novel coronavirus named SARS-CoV-2 has been causing devastating severe respiratory infections in human population worldwide. The new emerging disease was called COVID-19 and, as early as the beginning of 2020, the world found itself in a COVID 19 pandemic. Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and rapid transmission during the COVID-19 pandemic has enabled it to acquire significant genetic diversity since it first entered the human population. This led to the emergence of numerous variants, some of them recently being labeled, "variants of concern" (VOC). Emerging SARS-CoV-2 variants can be problematic if one or more of the independent mutations result in changes that make the virus more pathogenic, resistant to treatment, able to escape vaccines, or able to evade diagnostic tests. So far, four VOCs have been globally recognized (Alpha or B.1.1.7, Beta or B.1.35...
Gene polymorphisms result from evolutionary processes representing mutations that survive in the ... more Gene polymorphisms result from evolutionary processes representing mutations that survive in the population with a frequency higher than 1%. The most investigated type of gene polymorphisms are single nucleotide polymorphisms (SNPs). The SNPs of IL-12B (rs 3212227) A/C among a population of kidney graft CMV-seropositive recipients have an impact on a clinical events in cytomegalovirus (CMV) disease. Constitutive-308 G/A TNF-α polymorphism (rs1800629) is related to the susceptibility of HR-HPV-associated cervical dysplasia and cancer. SNP located 3 kb upstream of the IL-28B gene (rs12979860) seems to be the strongest host genetic predictor of sustained virologic response (SVR) in hepatitis C genotype 1 patients. It is very important to identify viral and host genetic markers that may facilitate the risk of developing viral disease or some viral-associated cancers. In addition, these markers could be useful in the choice of effective treatments and preventive strategies against virally induced infection.
Until now, the treatment protocols for COVID‐19 have been revised multiple times. The use and app... more Until now, the treatment protocols for COVID‐19 have been revised multiple times. The use and approval of therapeutic monoclonal antibodies (mAbs) for COVID‐19 treatment represent exceptional achievements in modern science, technology and medicine. SARS‐CoV‐2 Omicron evasion of pre‐existing immunity represents a serious public health problem nowadays. This systematic review with meta‐analysis provided comprehensive and up‐to‐date evidence of the clinical efficacy of therapeutic anti‐SARS‐CoV‐2 mAbs against Omicron subvariants in COVID‐19 patients and included 10 articles. The prevalence of hospitalisation among Omicron‐positive patients treated with anti‐SARS‐CoV‐2 mAbs was 2.8% (89/3169) while it controls (Omicron‐positive patients treated with other therapies) 11% (154/1371). There was a statistically significantly different number of hospitalisations between the two studied groups in favour of the anti‐SARS‐CoV‐2 mAbs treated group. (OR = 0.56, 95% CI OR = 0.41–0.77, p < 0.001...
Introduction/Objective. The aim of this study was to investigate the distribution of genotypes an... more Introduction/Objective. The aim of this study was to investigate the distribution of genotypes and alleles of proinflammatory cytokines TNF, IFN-?, and IL-12 and their effect on the development of a cervical illness and also to determine their associated influence with cofactors in HR HPV-positive women in Serbia. Methods. We have investigated 24 women and based on the cytological findings they were classified into four groups: PAP II, ASCUS, LSIL, and HSIL. Analysis of TNF, IL-12, and IFN-? polymorphisms was performed using the real-time PCR TaqMan method. Statistical analysis was performed using parametric and non-parametric tests and correlation and multiple regression analysis. Results. Significantly higher frequency of high production-related TNF AA genotype was observed in severe dysplasia. The correlation between TNF gene polymorphism and cervical findings were highly significant. There was a moderate, significant correlation between low production IFN-? AA genotype and earli...
The Journal of Infection in Developing Countries, 2020
Introduction: Hepatitis C Virus (HCV) is the leading cause of chronic liver disease and is a seri... more Introduction: Hepatitis C Virus (HCV) is the leading cause of chronic liver disease and is a serious global health problem. Hepatitis C infection is highly prevalent in patients with end stage renal disease (ESRD), due to frequent exposure to blood and blood products, nosocomial transmission of HCV, and prolong hemodialysis duration. The aim of the study was to evaluate the influence of IL-33/ST2 signaling pathway on severity of the liver disease in ESRD HCV+ patients. Methodology: Blood samples from patients with end stage renal disease (ESRD) and hepatitis C infection (HCV), 20 patients with HCV infection, 20 patients with ESRD and 20 healthy control donor patients were taken for the examination of biochemical parameters, for the determination of the serum cytokine concentration, and for the molecular diagnostics of HCV. Results: Systemic sST2 positively correlated with serum level of urea and creatinine, respectively. Serum sST2 was significantly increased in ESRD HCV+ patients i...
Ove godine, Nobelova nagrada za medicinu dodeljena je trojici naučnika koji su pre 31 godinu iden... more Ove godine, Nobelova nagrada za medicinu dodeljena je trojici naučnika koji su pre 31 godinu identifikovali novi, krvlju prenosivi primarno-hepatotropni virus (engl. blood born virus-BBV). Bio je to hepatis C virus (HCV), uzročnik istoimene infekcije i globalnog zdravstvenog problema koji se povezuje sa teškim komplikacijama, cirozom jetre i hepatocelularnim karcinomom (HCK). Zbog ovog velikog otktića trojice laureta ovogodišnje Nobelove nagrade – Harvija Altera, Majkla Hotona i Čarlsa Rajsa, kojim su zadužili čitavo čovečanstvo, nezamislivo je da se, u godini kada im je dodeljeno najprestižnije priznanje za otkrića u nauci, ne osvrnemo na njihov rad [1]. Hepatitis C virus je nova paradigma za identifikaciju i kontrolu virusnih infekcija, a njegovo otkriće je postalo prekretnica u borbi protiv virusnih bolesti tokom 20. veka. Od trenutka kada je HCV, kao novi BB hepatotropni virus identifikovan, pokrenuta je era naglog razvoja veoma osetljivih laboratorijskih testova za njegovo doka...
Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and r... more Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and rapid transmission during the COVID-19 pandemic has enabled it to acquire significant genetic diversity since it first entered the human population. This led to the emergence of numerous variants, some of them recently being labeled “variants of concern” (VOC), due to their potential impact on transmission, morbidity/mortality, and the evasion of neutralization by antibodies elicited by infection, vaccination, or therapeutic application. The potential to evade neutralization is the result of diversity of the target epitopes generated by the accumulation of mutations in the spike protein. While three globally recognized VOCs (Alpha or B.1.1.7, Beta or B.1.351, and Gamma or P.1) remain sensitive to neutralization albeit at reduced levels by the sera of convalescent individuals and recipients of several anti-COVID19 vaccines, the effect of spike variability is much more evident on the neutra...
Clinical significance of the cytomegalovirus (CMV) genotypes in patients undergoing allogeneic he... more Clinical significance of the cytomegalovirus (CMV) genotypes in patients undergoing allogeneic hematopoietic stem cell transplant (HSCT) has been evaluated mostly in adults. The studies of diverse CMV glycoprotein B (gB) and N (gN) genotype variants in transplanted children and adolescents are lacking. We analyzed the investment of gB and gN genotype variants in the HSCTed children and their relation to clinical complications and disease outcome. The cohort included forty two pediatric recipients of the HSCT. Patients positive for CMV DNAemia (24/42, 57.1%) were genotyped. The gB4 and gN1 genotype variants predominated and were evidenced in 7/18 (38.9%) and 9/19 (47.4%) patients, respectively. The graft-versus-host disease (GvHD) predominated in children with viremia (p < 0.05). Frequencies of the gB and gN genotypes contrasted those reported in recent studies. The GvHD scaled strongly with CMV reactivation whereas viral loads were uncorrelated to medical complications and treatment outcomes.
Accumulating evidence suggests that hepatitis B virus (HBV) biological features may influence the... more Accumulating evidence suggests that hepatitis B virus (HBV) biological features may influence the course and clinical manifestations of infection and possibly the development of fulminant hepatitis (FH). Since HBV is not a cytocidal virus, virus-induced liver damage results from an interplay between the virus replication and the host's defense. Therefore, viral factors contributing to enhanced replication, induction of a stronger immune attack or apoptosis of hepatocytes could be crucial in development of FH. Numerous mutations in basal core promoter, pre-C, C and S regions of the HBV genome contribute to development of FH by different mechanisms, including enhanced viral replication, the loss of a decoy for immune response, unbalanced expression of viral proteins and retention of unprocessed cytotoxic proteins in hepatocytes.
Introduction: Viruses are the most important and common cause of opportunistic infections followi... more Introduction: Viruses are the most important and common cause of opportunistic infections following transplantation. The risk correlates with the virus encountered, transplanted tissue and organ, intensity of immune suppression, and other host factors governing susceptibility. Infections caused by the human herpesviruses, continue to challenge the clinical management of transplant recipients. Aim: The aim of this study was to investigate the prevalence of herpesvirus infections among pediatric hematopoetic stem cell and renal transplant recipients (HSCTR and RTR). Material and methods: This is a retrospective study of 150 pediatric HSCTR and RTR investigated in plasma samples by PCR in multiple testings, on the presence of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus type 1 and 2 (HSV1/2) and human herpes virus 6 (HHV6) during 2015/2016 period. Visualization of PCR products was performed by electrophoresis on 2% agarose gel with ethidium bromide. For statistical analyses T test, McNemar's test, Chi-square and Fisher's exact test were used. Results: During 2015, statistical significance was reached at the follow ups, where 33.3% (p=0.031) and 46.7% (p=0.016) of HSCTR, and 4.3% and 28.0% of RTR, had positive CMV and EBV results, respectively, in regard to the first test. During 2016, similar finding was observed where HSCT recipients had 70.6% CMV (p=0.002) and 29.4% EBV positive results during the follow ups. Cytomegalovirus (CMV) finding was negative in all RTR, but 12.5 and 4.0% of investigated kidney recipients were EBV positive during the first test and follow ups, respectively. Conclusion: The results demonstrated that HSCTR are in a greater risk of CMV and EBV infections, compared to RTR. Therefore, the importance of permanent post-transplant monitoring of herpesviruses is in timely diagnosis and prevention of overt infections from occurring. Medicinski podmladak / Medical Youth Markolović M. et al. The prevalence of herpesvirus infections in children and young adults transplant recipients-kidney and hematopoetic stem cells.
Hepatitis B virus (HBV) reactivation occurs as a major complication of immunosuppressive therapy ... more Hepatitis B virus (HBV) reactivation occurs as a major complication of immunosuppressive therapy among persons who have recovered from acute hepatitis and those who have controlled chronic infection. Recent literature data emphasize the presence of a high degree of S gene variability in HBV isolates from patients who developed reactivation. In reactivated HBV, the most frequently detected mutations belong to the second loop of “a” determinant in HBsAg. These mutations were identified to be immune escape and responsible for vaccine- and diagnostic-escape phenomena. Their emergence clearly provides survival in the presence of a developed humoral immune response and is often associated with impaired serological diagnosis of HBV reactivation. The knowledge of their existence and roles can elucidate the process of reactivation and strongly highlights the importance of HBV DNA detection in monitoring all patients with a history of HBV infection who are undergoing immunosuppression. This r...
Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk... more Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk-HPV (HR-HPV) is necessary but not a sufficient aetiological agent for the development of cervical neoplasia. A number of viral, host, environmental and behavioural factors are suggested to be associated with the progression of cervical disorder. This study aimed to evaluate the impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia. A total of 541 women have been tested by PCR for the presence of HPV on the cervix. HPV genotypes were determined by direct DNA sequencing. Women identified as HR-HPV-positive were further classified into four subgroups according to their cytological status. All relevant information about demographical and behavioural factors was obtained by interviewer-based questionnaire. A number of analytical and descriptive statistical methods were used for processing the data. The cofactors found to...
Journal of Pediatric Hematology Oncology, Aug 1, 2012
We report a 2.5-year-old boy with an X-linked lymphoproliferative disease (XLP) phenotype who pre... more We report a 2.5-year-old boy with an X-linked lymphoproliferative disease (XLP) phenotype who presented with human herpes virus-8 (HHV-8)-related hemophagocytic lymphohistiocytosis (HLH). XLP is a rare primary immunodeficiency characterized by extreme susceptibility to herpes viruses, mainly Epstein-Barr virus (EBV). Approximately 60% of patients with XLP present with fulminant mononucleosis associated with HLH, whereas remaining patients present with hypogammaglobulinemia or lymphoproliferative disease. Most commonly, one of the XLP phenotypes appears after exposure to EBV, but at least 12% of affected individuals developed symptoms without an evidence of EBV infection. Rarely, patients with XLP may present with central nervous system vasculitis or aplastic anemia. HHV-8 is lymphotrophic and it is associated with lymphoproliferative disorders and Kaposi sarcoma in immunodeficient hosts. Kaposi sarcoma rarely occurs in children with well-defined primary immunodeficiency. Also, HHV-8-related HLH was previously reported in 2 siblings with a perforin gene deficiency. Recently, it became evident that besides EBV, other viruses may trigger the symptoms in XLP. We report for the first time HHV-8-related HLH in EBV-negative pediatric patient with an XLP phenotype.
Since emerging from Wuhan, China, in December of 2019, the novel coronavirus named SARS-CoV-2 has... more Since emerging from Wuhan, China, in December of 2019, the novel coronavirus named SARS-CoV-2 has been causing devastating severe respiratory infections in human population worldwide. The new emerging disease was called COVID-19 and, as early as the beginning of 2020, the world found itself in a COVID 19 pandemic. Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and rapid transmission during the COVID-19 pandemic has enabled it to acquire significant genetic diversity since it first entered the human population. This led to the emergence of numerous variants, some of them recently being labeled, "variants of concern" (VOC). Emerging SARS-CoV-2 variants can be problematic if one or more of the independent mutations result in changes that make the virus more pathogenic, resistant to treatment, able to escape vaccines, or able to evade diagnostic tests. So far, four VOCs have been globally recognized (Alpha or B.1.1.7, Beta or B.1.35...
Gene polymorphisms result from evolutionary processes representing mutations that survive in the ... more Gene polymorphisms result from evolutionary processes representing mutations that survive in the population with a frequency higher than 1%. The most investigated type of gene polymorphisms are single nucleotide polymorphisms (SNPs). The SNPs of IL-12B (rs 3212227) A/C among a population of kidney graft CMV-seropositive recipients have an impact on a clinical events in cytomegalovirus (CMV) disease. Constitutive-308 G/A TNF-α polymorphism (rs1800629) is related to the susceptibility of HR-HPV-associated cervical dysplasia and cancer. SNP located 3 kb upstream of the IL-28B gene (rs12979860) seems to be the strongest host genetic predictor of sustained virologic response (SVR) in hepatitis C genotype 1 patients. It is very important to identify viral and host genetic markers that may facilitate the risk of developing viral disease or some viral-associated cancers. In addition, these markers could be useful in the choice of effective treatments and preventive strategies against virally induced infection.
Until now, the treatment protocols for COVID‐19 have been revised multiple times. The use and app... more Until now, the treatment protocols for COVID‐19 have been revised multiple times. The use and approval of therapeutic monoclonal antibodies (mAbs) for COVID‐19 treatment represent exceptional achievements in modern science, technology and medicine. SARS‐CoV‐2 Omicron evasion of pre‐existing immunity represents a serious public health problem nowadays. This systematic review with meta‐analysis provided comprehensive and up‐to‐date evidence of the clinical efficacy of therapeutic anti‐SARS‐CoV‐2 mAbs against Omicron subvariants in COVID‐19 patients and included 10 articles. The prevalence of hospitalisation among Omicron‐positive patients treated with anti‐SARS‐CoV‐2 mAbs was 2.8% (89/3169) while it controls (Omicron‐positive patients treated with other therapies) 11% (154/1371). There was a statistically significantly different number of hospitalisations between the two studied groups in favour of the anti‐SARS‐CoV‐2 mAbs treated group. (OR = 0.56, 95% CI OR = 0.41–0.77, p < 0.001...
Introduction/Objective. The aim of this study was to investigate the distribution of genotypes an... more Introduction/Objective. The aim of this study was to investigate the distribution of genotypes and alleles of proinflammatory cytokines TNF, IFN-?, and IL-12 and their effect on the development of a cervical illness and also to determine their associated influence with cofactors in HR HPV-positive women in Serbia. Methods. We have investigated 24 women and based on the cytological findings they were classified into four groups: PAP II, ASCUS, LSIL, and HSIL. Analysis of TNF, IL-12, and IFN-? polymorphisms was performed using the real-time PCR TaqMan method. Statistical analysis was performed using parametric and non-parametric tests and correlation and multiple regression analysis. Results. Significantly higher frequency of high production-related TNF AA genotype was observed in severe dysplasia. The correlation between TNF gene polymorphism and cervical findings were highly significant. There was a moderate, significant correlation between low production IFN-? AA genotype and earli...
The Journal of Infection in Developing Countries, 2020
Introduction: Hepatitis C Virus (HCV) is the leading cause of chronic liver disease and is a seri... more Introduction: Hepatitis C Virus (HCV) is the leading cause of chronic liver disease and is a serious global health problem. Hepatitis C infection is highly prevalent in patients with end stage renal disease (ESRD), due to frequent exposure to blood and blood products, nosocomial transmission of HCV, and prolong hemodialysis duration. The aim of the study was to evaluate the influence of IL-33/ST2 signaling pathway on severity of the liver disease in ESRD HCV+ patients. Methodology: Blood samples from patients with end stage renal disease (ESRD) and hepatitis C infection (HCV), 20 patients with HCV infection, 20 patients with ESRD and 20 healthy control donor patients were taken for the examination of biochemical parameters, for the determination of the serum cytokine concentration, and for the molecular diagnostics of HCV. Results: Systemic sST2 positively correlated with serum level of urea and creatinine, respectively. Serum sST2 was significantly increased in ESRD HCV+ patients i...
Ove godine, Nobelova nagrada za medicinu dodeljena je trojici naučnika koji su pre 31 godinu iden... more Ove godine, Nobelova nagrada za medicinu dodeljena je trojici naučnika koji su pre 31 godinu identifikovali novi, krvlju prenosivi primarno-hepatotropni virus (engl. blood born virus-BBV). Bio je to hepatis C virus (HCV), uzročnik istoimene infekcije i globalnog zdravstvenog problema koji se povezuje sa teškim komplikacijama, cirozom jetre i hepatocelularnim karcinomom (HCK). Zbog ovog velikog otktića trojice laureta ovogodišnje Nobelove nagrade – Harvija Altera, Majkla Hotona i Čarlsa Rajsa, kojim su zadužili čitavo čovečanstvo, nezamislivo je da se, u godini kada im je dodeljeno najprestižnije priznanje za otkrića u nauci, ne osvrnemo na njihov rad [1]. Hepatitis C virus je nova paradigma za identifikaciju i kontrolu virusnih infekcija, a njegovo otkriće je postalo prekretnica u borbi protiv virusnih bolesti tokom 20. veka. Od trenutka kada je HCV, kao novi BB hepatotropni virus identifikovan, pokrenuta je era naglog razvoja veoma osetljivih laboratorijskih testova za njegovo doka...
Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and r... more Despite the slow evolutionary rate of SARS-CoV-2 relative to other RNA viruses, its massive and rapid transmission during the COVID-19 pandemic has enabled it to acquire significant genetic diversity since it first entered the human population. This led to the emergence of numerous variants, some of them recently being labeled “variants of concern” (VOC), due to their potential impact on transmission, morbidity/mortality, and the evasion of neutralization by antibodies elicited by infection, vaccination, or therapeutic application. The potential to evade neutralization is the result of diversity of the target epitopes generated by the accumulation of mutations in the spike protein. While three globally recognized VOCs (Alpha or B.1.1.7, Beta or B.1.351, and Gamma or P.1) remain sensitive to neutralization albeit at reduced levels by the sera of convalescent individuals and recipients of several anti-COVID19 vaccines, the effect of spike variability is much more evident on the neutra...
Clinical significance of the cytomegalovirus (CMV) genotypes in patients undergoing allogeneic he... more Clinical significance of the cytomegalovirus (CMV) genotypes in patients undergoing allogeneic hematopoietic stem cell transplant (HSCT) has been evaluated mostly in adults. The studies of diverse CMV glycoprotein B (gB) and N (gN) genotype variants in transplanted children and adolescents are lacking. We analyzed the investment of gB and gN genotype variants in the HSCTed children and their relation to clinical complications and disease outcome. The cohort included forty two pediatric recipients of the HSCT. Patients positive for CMV DNAemia (24/42, 57.1%) were genotyped. The gB4 and gN1 genotype variants predominated and were evidenced in 7/18 (38.9%) and 9/19 (47.4%) patients, respectively. The graft-versus-host disease (GvHD) predominated in children with viremia (p < 0.05). Frequencies of the gB and gN genotypes contrasted those reported in recent studies. The GvHD scaled strongly with CMV reactivation whereas viral loads were uncorrelated to medical complications and treatment outcomes.
Accumulating evidence suggests that hepatitis B virus (HBV) biological features may influence the... more Accumulating evidence suggests that hepatitis B virus (HBV) biological features may influence the course and clinical manifestations of infection and possibly the development of fulminant hepatitis (FH). Since HBV is not a cytocidal virus, virus-induced liver damage results from an interplay between the virus replication and the host's defense. Therefore, viral factors contributing to enhanced replication, induction of a stronger immune attack or apoptosis of hepatocytes could be crucial in development of FH. Numerous mutations in basal core promoter, pre-C, C and S regions of the HBV genome contribute to development of FH by different mechanisms, including enhanced viral replication, the loss of a decoy for immune response, unbalanced expression of viral proteins and retention of unprocessed cytotoxic proteins in hepatocytes.
Introduction: Viruses are the most important and common cause of opportunistic infections followi... more Introduction: Viruses are the most important and common cause of opportunistic infections following transplantation. The risk correlates with the virus encountered, transplanted tissue and organ, intensity of immune suppression, and other host factors governing susceptibility. Infections caused by the human herpesviruses, continue to challenge the clinical management of transplant recipients. Aim: The aim of this study was to investigate the prevalence of herpesvirus infections among pediatric hematopoetic stem cell and renal transplant recipients (HSCTR and RTR). Material and methods: This is a retrospective study of 150 pediatric HSCTR and RTR investigated in plasma samples by PCR in multiple testings, on the presence of cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus type 1 and 2 (HSV1/2) and human herpes virus 6 (HHV6) during 2015/2016 period. Visualization of PCR products was performed by electrophoresis on 2% agarose gel with ethidium bromide. For statistical analyses T test, McNemar's test, Chi-square and Fisher's exact test were used. Results: During 2015, statistical significance was reached at the follow ups, where 33.3% (p=0.031) and 46.7% (p=0.016) of HSCTR, and 4.3% and 28.0% of RTR, had positive CMV and EBV results, respectively, in regard to the first test. During 2016, similar finding was observed where HSCT recipients had 70.6% CMV (p=0.002) and 29.4% EBV positive results during the follow ups. Cytomegalovirus (CMV) finding was negative in all RTR, but 12.5 and 4.0% of investigated kidney recipients were EBV positive during the first test and follow ups, respectively. Conclusion: The results demonstrated that HSCTR are in a greater risk of CMV and EBV infections, compared to RTR. Therefore, the importance of permanent post-transplant monitoring of herpesviruses is in timely diagnosis and prevention of overt infections from occurring. Medicinski podmladak / Medical Youth Markolović M. et al. The prevalence of herpesvirus infections in children and young adults transplant recipients-kidney and hematopoetic stem cells.
Hepatitis B virus (HBV) reactivation occurs as a major complication of immunosuppressive therapy ... more Hepatitis B virus (HBV) reactivation occurs as a major complication of immunosuppressive therapy among persons who have recovered from acute hepatitis and those who have controlled chronic infection. Recent literature data emphasize the presence of a high degree of S gene variability in HBV isolates from patients who developed reactivation. In reactivated HBV, the most frequently detected mutations belong to the second loop of “a” determinant in HBsAg. These mutations were identified to be immune escape and responsible for vaccine- and diagnostic-escape phenomena. Their emergence clearly provides survival in the presence of a developed humoral immune response and is often associated with impaired serological diagnosis of HBV reactivation. The knowledge of their existence and roles can elucidate the process of reactivation and strongly highlights the importance of HBV DNA detection in monitoring all patients with a history of HBV infection who are undergoing immunosuppression. This r...
Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk... more Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk-HPV (HR-HPV) is necessary but not a sufficient aetiological agent for the development of cervical neoplasia. A number of viral, host, environmental and behavioural factors are suggested to be associated with the progression of cervical disorder. This study aimed to evaluate the impact of environmental and behavioural cofactors on the development of cervical disorders in HR-HPV-infected women in Serbia. A total of 541 women have been tested by PCR for the presence of HPV on the cervix. HPV genotypes were determined by direct DNA sequencing. Women identified as HR-HPV-positive were further classified into four subgroups according to their cytological status. All relevant information about demographical and behavioural factors was obtained by interviewer-based questionnaire. A number of analytical and descriptive statistical methods were used for processing the data. The cofactors found to...
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