Papers by Mahmood Jeddi-tehrani
Avicenna journal of medical biotechnology, Jun 18, 2023
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
International Immunopharmacology, Aug 1, 2023
Journal of Assisted Reproduction and Genetics, Nov 6, 2013
Purpose To compare plasminogen activator inhibitor type1 (PAI-1) mutation rates in different grou... more Purpose To compare plasminogen activator inhibitor type1 (PAI-1) mutation rates in different groups of patients with the record of recurrent miscarriage (RM) or implantation failure (IF) with special emphasis on the number of missed pregnancies and/or implantation failures (RM≥2, IF≥2, RM + IF≥2, RM≥3, IF≥3 and RM + IF≥3). Method Case-control study from PCR products and RFLP data of DNA from blood of patients who referred to the infertility clinic including 595 patients (421 RM≥2, 119 IF≥2 and 55 RM + IF≥2) as the case groups and 100 healthy women as the control group. Results All six different subgroups of patients showed increased frequencies of the mutant allele (4G) in comparison to the control group (p <0.001) suggesting a role for PAI-1 mutation in RM and IF. Conclusions The different patient subgroups suffer similar rates of risk in developing RM and IF when compared to controls.
Authorea (Authorea), May 28, 2021
Given the emergence of SARS-CoV-2 virus as a life-threatening pandemic, identification of immunod... more Given the emergence of SARS-CoV-2 virus as a life-threatening pandemic, identification of immunodominant epitopes of the viral structural proteins, particularly the nucleocapsid (NP) protein and receptor binding domain (RBD) of spike protein, is important to determine targets for immunotherapy and diagnosis. In this study, epitope screening was performed using a panel of overlapping peptides spanning the entire sequences of the RBD and NP proteins of SARS-CoV-2 in the sera from 66 COVID-19 patients and 23 healthy subjects by enzyme-linked immunosorbent assay (ELISA). Also, three non-overlapping peptides belonging to the S2 domain of spike protein were assessed. Our results showed that while reactivity of patients' sera with reduced recombinant RBD protein was significantly lower than the native form of RBD (p<0.001), no significant differences were observed for reactivity of patients' sera with reduced and non-reduced NP protein. Pepscan analysis revealed weak to moderate reactivity towards different RBD peptide pools, which was more focused on peptides encompassing aa 181-223 of RBD. NP peptides, however, displayed strong reactivity with a single peptide covering aa 151-170. These findings were confirmed by peptide depletion experiments using both ELISA and Western blotting. Altogether, our data suggest the involvement of mostly conformational disulfide bond-dependent immunodominant epitopes in RBD-specific antibody response, while the IgG response to NP is dominated by linear epitopes. Identification of antigenic epitope in NP and RBD of SARS-CoV-2 could provide important advances for the development of passive and active immunotherapy as well as diagnostic tools for the control of COVID-19 infection.
JOURNAL OF REPRODUCTION AND INFERTILITY, 2000
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection, with an est... more Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection, with an estimated annual worldwide incidence of 50 million cases. A large Proportion of those infected, particularly women, are asymptomatic, and these individuals serve as a major reservoir of infection. Women are also at risk for serious reproductive tract complications with significant morbidity. In an effort to prevent spread of these infections, increased attention is being paid to early diagnosis and treatment. The introduction of sensitive and highly specific nucleic acid amplification tests for detection of C. trachomatis has made the use of noninvasive testing feasible in women. Recent studies have found that nucleic acid amplification tests are sufficiently sensitive to detect C. trachomatis in first-void urine in women. Sensitivities have exceeded 95% in most studies when compared to detection with non culture tests of endocervical specimens as a standard, while at the same time preserving high specificities
PubMed, 1993
A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody pro¬ duced against the c... more A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody pro¬ duced against the colon cancer cell line SW1116. By immunohistochemical stai¬ ning the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (I0R-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characteri¬ zation indicate that at least two I0R-C2 reactive sites are present on the an¬ tigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of I0R-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is dis¬ tinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.
Research in Pharmaceutical Sciences, Aug 30, 2012
Background and Aims: Monoclonal antibodies (mAbs) are currently among the fastest growing classes... more Background and Aims: Monoclonal antibodies (mAbs) are currently among the fastest growing classes of therapeutic entities. Owning to such efforts, adequate delivery systems to preserve their activity and control their release are required. Although injectable polymeric nanoparticles are among the best candidates to reach this purpose, antibody instabilities have been the major hindrance. Most of the papers in this field has just concentrated on structural stability of proteins loaded in polymers such as poly(lactic-co-glycolic) acid (PLGA) rather than their activity. Methods: In this study we have investigated the activity of bevacizumab, a model antibody drug, during encapsulation into PLGA in presence and absence of stabilizers. Nanoparticles were prepared by double emulsion method. The effects of organic solvents, homogenization techniques and different stabilizers (mannitol, trehalose, glycine, PEG (300, 1000 and 4000 D), polysorbate 80, and bovine serum albumin (BSA), polyvinyl alcohol) on the activity of bevacizumab were evaluated by enzyme-linked immunosorbent assay (ELISA) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Results: Results suggest that all of examined organic solvents cause a considerable reduction in antibody activity. Presence of interfaces between aqueous and organic phase, rather than shear stress during homogenizing, is the main reason of protein denaturation and inactivation in emulsion methods. Up to now, complete release of totally intact protein has not been achieved even in optimized encapsulation processes. We revealed that presence of 10% w/v BSA in internal aqueous phase during primary emulsification was an effective strategy to preserve the activity of bevacizumab. Conclusions: Finally it can be concluded that this systematic process can be use to produce efficient sustained release nanoparticles for antibodies as well as other therapeutic proteins.
PubMed, Jun 1, 2005
In spite of their key role in various immunological processes occurring in the endometrium, T cel... more In spite of their key role in various immunological processes occurring in the endometrium, T cells- especially ab+ subtype- residing in this mucosal tissue, have not been extensively explored. We present here the profile of expressed genes for variable region of b chain of T cell receptor (TCR) in normal endometrium as compared to peripheral blood. Samples from endometrium were taken from normal fertile women during routine check-up by Pipelle pipette or after hysterectomy operation. Total RNA from both blood and endometrial samples was extracted and RT-PCR using BV gene specific primers was performed. After southern blotting, hybridization with radiolabelled specific probe and autoradiography, relative expression of each BV family was determined. Clonal expansions of the over-expressed genes were studied by determining their CDR3 length polymorphism. A total of 12 blood and 14 endometrial samples were collected. Only one TCRBV gene (TCRBV7) was expressed significantly more and 3 genes less frequently in the endometrium compared to blood. Also, two other genes (TCRBV10 and 12) were found marginally more frequent in the endometrium. As for their clonality, all 3 TCRBV genes examined here showed a rather restricted (oligoclonal) and in some cases, very restricted (probably monoclonal) pattern in the endometrium in contrast to polyclonal patterns in the blood. Our results indicate the similarities between T cells residing in different mucosal tissues and support their common recruitment and functional potentials. Moreover, our findings provide a basis for future investigations about endometrial T cell involvement and their antigen specificities in different gynecological problems.
DOAJ (DOAJ: Directory of Open Access Journals), Nov 1, 2014
Background: Varicella zoster virus (VZV) is a member of herpes family viruses, which causes varic... more Background: Varicella zoster virus (VZV) is a member of herpes family viruses, which causes varicella (chickenpox) after primary infection and herpes zoster (shingles) because of latent virus reactivation from dorsal root ganglia. Generally, prevalence of varicella antibodies increases with age. We aimed to compare the prevalence of anti-VZV antibody in children under seven years old, in order to obtain a preliminarily picture of general presence of these antibodies to design an immunization plan. Methods: In this cross-sectional study, performed from September 2011 to September 2012 in Tehran , Iran, 267 serum samples including sera from 7 month old infants, n= 87; 18 month old children, n= 86; and 6 year old children, n= 94 were assessed for the presence of specific IgG antibodies against VZV, using ELISA technique. Results: 4.6% of 7 month, 12.8% of 18 month and 21.3% of 6-year-old children were seropositive. No relation was found between demographic variables (e.g. age and birth weight) and seropositivity in these age groups. VZV antibodies increased with age. Serum levels of varicella antibodies were elevated in 18 months old compared to 7 months old children, significantly (P < 0.001). Conclusion: In view of the significant elevation of VZV antibodies in children from 7 months to 18 months of age and rate of seronegative children, our results support the necessity of varicella immunization between 7 and 18 months of age in order to prevent viral infection.
PubMed, Jun 1, 2018
Antibodies are proteins of the immune system that are produced by B-lymphocytes. These proteins e... more Antibodies are proteins of the immune system that are produced by B-lymphocytes. These proteins exert their effects by recognizing and binding to their targets (antigens). A monoclonal antibody (mAb) is originally produced by a single B-cell. Production of mAbs was first introduced in 1975 using cell fusion technique and hybridoma cell production. A Hybridoma is formed by fusion of an antibody producing B-lymphocyte and a myeloma cell line. These cells have two main characteristics, production of uniform, monospecific antibodies (mAbs) that originate from the B-cell and immortality that comes from the myeloma cell line. A hybridoma cell line is thus acting like a biological factory that produces and secretes mAbs into the cell culture medium. Most of the mAbs have been produced in mice and are thus proteins of murine origin. mAbs were later found to be able to bind biological targets like tumor antigens, molecules involved in autoimmune and infectious disease-related molecules, etc. This led to emergence of therapeutic monoclonal antibodies.
Scandinavian Journal of Immunology, May 1, 2003
One to 10 per cent of healthy adult individuals do not produce protective levels of anti-hepatiti... more One to 10 per cent of healthy adult individuals do not produce protective levels of anti-hepatitis B surface (HBs) antibodies, following a standard vaccination protocol. Lack of an HBs antigen (Ag)-specific T-cell repertoire is amongst the possible defects, which may lead to humoral unresponsiveness and is the main objective of this study. We analysed TcR BV (T-cell receptor beta chain variable) gene usage in T lymphocytes from nine healthy adult responders and six nonresponders to recombinant HB vaccine, before and after booster vaccination. CD4+ and CD8+ T-cell populations were isolated from peripheral blood mononuclear cells by magnetic beads, and the expression of TcR BV genes in each population was investigated by reverse transcription polymerase chain reaction and hybridization with specific probe. When the usage of each TcR BV gene within CD4+ and CD8+ T cells of the responders was compared with that of nonresponders, statistically significant difference (P &lt; 0.01) was noted for BV5S2-3 gene family in CD4+ T cells of nonresponders. Furthermore, individual vaccinees were shown to overexpress several TcR BV genes. To characterize the T-cell repertoire and determine their clonal nature, analysis of CDR3 length polymorphism was performed. Our results show that T-cell response to HBsAg is generally oligoclonal and involves multiple BV families. Furthermore, overexpressed individual TcR BV genes and CDR3 length distributions in response to HBsAg are subject-dependent. In conclusion, our results are not in line with the notion that defective TcR repertoire may be an explanation for unresponsiveness to recombinant HBsAg vaccine.
Hybridoma, Oct 1, 2012
Leptin is an important protein that regulates energy storage and homeostasis in humans and animal... more Leptin is an important protein that regulates energy storage and homeostasis in humans and animals. Leptin deficiency results in various abnormalities such as diabetes, obesity, and infertility. Producing a high affinity monoclonal antibody against human leptin provides an important tool to monitor and trace leptin function in different biological fluids. In this study, recombinant human leptin was conjugated to KLH and injected into mice. After immunization, mouse myeloma SP2/0 cells were fused with murine splenocytes followed by selection of antibody-producing hybridoma cells. After screening of different hybridoma colonies by ELISA, a high affinity antibody was selected and purified by affinity chromatography. The affinity constant of the antibody was measured by ELISA. Western blot, immunocytochemistry, and flow cytometry experiments were used to characterize the antibody. The anti-leptin antibody had a high affinity (around 1.13 × 10(-9) M) for its antigen. The saturation of the antibody with leptin (20 moles leptin per 1 mole antibody) in Western blot analysis proved that the antibody had specific binding to its antigen. Immunocytochemistry and flow cytometry on JEG-3 (human placental choriocarcinoma cell) cells revealed that the anti-leptin antibody recognized intracellular leptin. In conclusion, we report here the production and characterization of a murine anti-leptin antibody with high affinity for human leptin.
PubMed, Apr 26, 2016
Background: Methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) C6... more Background: Methylenetetrahydrofolate reductase (MTHFR) single-nucleotide polymorphisms (SNPs) C677T and A1298C have been described as strong risk factors for idiopathic recurrent miscarriage (RM). However, very few studies have investigated the association of paternal MTHFR SNPs with RM. The aim of the present study was to evaluate the prevalence of paternal C677T and A1298C SNPs among Iranian RM couples. Methods: The study subjects comprised 225 couples with more than three consecutive pregnancy losses, and 100 control couples with no history of pregnancy complications. All females in the case group had MTHFR polymorphisms; and genotype SNPs were analyzed by PCR-RFLP. Groups were statistically compared using Mann Whitney U-test and Chi-square statistical tests. The p<0.05 were considered significant. Results: Statistically significant difference was detected in the frequency of MTHFR SNPs in male partners of the two groups (p=0.019). Combined heterozygosity of MTHFR polymorphisms was a common phenomenon in the males; 52 (23.1%) and 14 (14%) of males in RM and control groups, respectively. Absence of combined homozygosity for both SNPs in all studied groups/genders was observed. Conclusion: The MTHFR gene composition of male partners of RM couples may contribute to increased risk of miscarriage.
Molecular Medicine, Jul 1, 1996
Background: The principal symptoms of myasthenia gravis (MG), muscde weakness and fatigue due to ... more Background: The principal symptoms of myasthenia gravis (MG), muscde weakness and fatigue due to impaired neuromuscular transmission, are caused by autoantibodies to the musde nicotinic acetylcholine receptor (AChR). The mechanisms underlying the autoimmnune response, however, appear to be initiated by activation of specific HLA dass II-restricted CD4+ T lymphocytes. Thus, central to elucidating the causation of MG is deternmining how T cells are recruited to contribute to misguided immunological assaults on the major autoantigenic target, AChR. Materials and Methods: By combining a polymerase chain reaction (PCR)-based strategy and Southern blot technique, we have analyzed the frequency of expression of 22 individual T cell receptor (TCR) VP gene subfamilies in CD4+ and CD8+ peripheral blood T cell subsets derived from eight MG patients and seven healthy controls. The quantification of relative usage of individual TCR Jo3 gene segments was performed by hybridization of PCR-amplified products (specifically V,Bl-C13) with a complete panel of 32P-5'-end-labeled Jo3-specific oligonucleotide probes, followed by scanning analysis of autoradiographs. Results: Comparisons of data obtained from VP3 analyses of T cells from MG patients with those from healthy individuals established that MG patients significantly overexpressed VPB1, VP313.2, VPB17, and V1320 gene family members within both CD4+ and CD8+ T cell subpopulations. Moreover, analysis of the relative utilization of individual TCR J,B gene segments in VPB1 +/CD4+ and V(31+/CD8+ T lymphocytes revealed distribution patterns in patients indistinguishable from those recorded in the corresponding cell subsets derived from controls. Conclusions: T lymphocytes from MG patients displayed a biased overexpression of four TCR VP3 gene segments: V,Bl, Vj313.2, V,B17, and Vf20. The relative frequencies of association of individual Vf3l (D,3)J,B combinations revealed that Jo gene usage in the V131-overrepresented T cell subsets had normal distribution patterns. It can thus be deduced that Jf gene segment products appear not to have a selective effect on the process leading to overexpression of Vo1 exons in MG patients. Hence, our observations suggest a possible role for superantigen(s) in the T cell activation in MG patients.
ACS Applied Materials & Interfaces, May 17, 2018
Designable coordination polymers with suitable chemical diversities and biocompatible structures ... more Designable coordination polymers with suitable chemical diversities and biocompatible structures have been proposed as a promising class of vehicles for drug delivery systems. Here, we hydrothermally synthesized a novel oned i m en s i on a l (1 D) co o r d i nat i o n p o ly m er , [Z n-(H 2 O) 6 K 2 (H 2 BTC) 2 (H 2 O) 4 ](H 2 BTC) 2 •2H 2 O, where H 3 BTC = benzene-1,3,5-tricarboxylic acid (trimesic acid), cp.1. As the hydrogen bonds stabilized 1D chains in three dimensions, the cp.1 could be a good candidate for delivering small-molecule chemotherapeutics such as 5-fluorouracil (5-Fu). The synthesized cp.1 showed a remarkable 5-Fu loading of 66% with encapsulation efficiency of 98% and almost complete release process. The 5-Fu-loaded cp.1 displayed a time-dependent cytotoxicity effect against breast cancer cell lines MCF-7 and 4T1. The cellular uptake of cp.1 particles was investigated via confocal laser scanning microscopy using fluorescein isothiocyanate and LysoTracker Red staining. Furthermore, the in vivo antitumor impact of 5-Fu-loaded cp.1 was studied on 4T1 breast cancer BALB/c mice model. The intratumor treatment of 5-Fu-loaded cp.1 demonstrated beneficial antitumor efficacy by postponing tumor growth. These results suggest that the 5-Fu-loaded cp.1 microparticles with a great locoregional delivery can be efficient anticancer drug carriers for further clinical treatments.
Journal of Medical Microbiology, Jun 30, 2023
All data generated or analyzed during this study are included in this published article.
Annals of Oncology, Sep 1, 2017
Monoclonal antibodies in immunodiagnosis and immunotherapy, Apr 1, 2020
Toxoplasma gondii is an intracellular protozoan parasite that can infect a wide range of warm-blo... more Toxoplasma gondii is an intracellular protozoan parasite that can infect a wide range of warm-blooded animals. Humans as an intermediate host are infected by ingesting infectious oocytes or tissue cysts, or passing through the placenta in pregnant women. The aim of this study is producing monoclonal antibodies against a synthetic peptide from (surface antigen 1 [SAG1] or P30) protein of T. gondii. A synthetic peptide from SAG1 (P30) protein was conjugated to Keyhole Limpet Hemocyanin (KLH (and then used for immunization of two BALB/c mice. The produced antibody was purified by affinity chromatography and its specific interaction with the immunized peptide was then determined by enzyme-linked immunosorbent assay (ELISA). Immunoreactivity of the antibody was also tested by Western blot in T. gondii cell lysate. The results show that the produced antibody has excellent reactivity with the immunizing peptide and also detects a single band of 30 kDa, which corresponds to SAG1 protein. This antibody can be used as a tool in different applications in T. gondii research areas, including diagnosis, therapy, and infection inhibition.
Blood, Apr 1, 1998
Idiotypic structures expressed on the myeloma Ig protein might be regarded as a tumor-specific an... more Idiotypic structures expressed on the myeloma Ig protein might be regarded as a tumor-specific antigen. Five patients with IgG myeloma were immunized with the purified serum M-component by repeated intradermal injections together with soluble granulocyte-macrophage colony-stimulating factor (GM-CSF). All patients developed an idiotype (Id)-specific T-cell immunity, defined as blood T cells predominantly secreting interferon-␥ (IFN-␥) and interleukin-2 (IL-2) (type I cells). Id-specific DNA synthesis was induced in one patient. Delayed-type hypersensitivity against the Id was not evoked. The specific IFN-␥/IL-2 T-cell response was inhibited (46% to 100%) by a major histocompatibility complex (MHC) class I monoclonal antibody (MoAb) in all five patients. A 5% to 37% inhibition by an MHC class II MoAb was seen in four patients. CD4 ؉ as well as CD8 ؉ T cells enriched by magnetic microbeads contained Id-specific cells. The T cells recognized peptides corresponding to the complementarity-determining regions 1, 2, and 3 of the heavy chain of the Id. There was a transient rise of B cells producing IgM anti-idiotypic antibodies in all patients. The results indicate that immunization of myeloma patients using the autologous M-component and soluble GM-CSF may evoke an Id-specific predominantly MHC class I-restricted type I T-cell response.
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Papers by Mahmood Jeddi-tehrani