Papers by Marija Viljevac Vuletić
Plants
The selection of drought-tolerant sour cherry genotypes is essential for developing sustainable f... more The selection of drought-tolerant sour cherry genotypes is essential for developing sustainable fruit production in today’s climate-change conditions. The phenotypic heterogenic population of sour cherry Oblačinska, with high and regular yield suitable for mechanical harvesting and industrial processing, is a traditional and predominant cultivar in northern Croatia (Pannonian region) and Serbia commercial orchards. In this context, 2-year old virus-free sour cherry plants of 4 isolated Oblačinska sour cherry ecotypes (OS, 18, D6, and BOR) produced by micropropagation were exposed to severe drought in a greenhouse under semi-controlled conditions to evaluate its photosynthetic intra-varietal variability. Relative water content (RWC), chlorophyll fluorescence (ChlF), and photosynthetic pigments were evaluated during the ten days of the experiment. As a visible symptom of stress, the withering of plants was followed by a diminution of RWC and photosynthetic pigments in the drought expo...
Poljoprivreda, 2019
Red clover is a perennial forage crop rich in isoflavones, the bioactive compounds with a positiv... more Red clover is a perennial forage crop rich in isoflavones, the bioactive compounds with a positive effect on human and animal health. The aim of the research was to determine a variation in the isoflavone contents in the Croatian red clover collection and to identify the most promising materials to be used in the breeding program for the development of new cultivars for specific purposes. Leaf samples of 29 red clover cultivars/populations (two cultivars, twenty breeding populations, and seven local populations) were collected in a full flowering stage, and the identification and quantification of isoflavones was performed using the HPLC analysis. The most common isoflavones in the red clover cultivars/populations were formononetin and biochanin A. A significant variation among the cultivars/populations in the total and individual isoflavone content was determined. The populations with very high and low contents of both the total and of the individual isoflavones were identified to ...
Agricultural Water Management
Applied Ecology and Environmental Research, 2018
Codling moth (Cydia pomonella L.) is one of the most important apple pests which population is st... more Codling moth (Cydia pomonella L.) is one of the most important apple pests which population is steadily growing. The aim of this study, conducted in Eastern Croatia, was to determine the efficiency of two biological preparations CpGV and kaolin clay compared with pyrethroids and control treatment. The preparations used in treatments were: 1) Cydia pomonella granulovirus (CpGV), 2) deltamethrin, beta cyfluthrin and alpha-cipemetrina, 3) kaolin, 4) control treatment, applying in two different methods of time control, on the three apple varieties 'Melrose', 'Jonagored' and 'Golden Delicious' clone B. The efficiency of the treatment 1 (CpGV) ranged from 78% to 95%, of treatment 2 (pyrethroids) from 96% to 97%, and treatment 3 (kaolin)between 21.5 and 57%. There was significant negative correlation between yield and damage (r = −07.56). Two methods of determining time control did not differ significantly. Variety 'Melrose' had the highest number of fruits damage in all three years and 'Golden Delicious' clon B the lowest number which can be related to the codling moth caterpillar affinity to a certain variety. It is very important to make the best choice of preparations in control and at the same time be sure of their positive effect on the environment and human health.
Horticulturae, 2022
The aim of this study was to evaluate physiological responses to short-term heat stress in the le... more The aim of this study was to evaluate physiological responses to short-term heat stress in the leaves of traditional (Bistrica) and modern (Toptaste) plum cultivars. In this study, detached plum leaves were incubated at 25 °C (control) and 40 °C (stress). After 1 h of exposure to heat (40 °C), chlorophyll a fluorescence transients were measured, and several biochemical parameters were analyzed. Elevated temperature caused heat stress in both plum cultivars, seen as a decrease in water content (WT), but in the leaves of the cultivar Bistrica, an accumulation of proline and phenols, as well as an accumulation of photosynthetic pigments, suggest the activation of a significant response to unfavorable conditions. Conversely, in the leaves of Toptaste, a significant accumulation of malondialdehyde (MDA) and an activation of guaiacol peroxidase (GPOD), all together with a decreased soluble proteins content, indicate an inadequate response to maintaining homeostasis in the leaf metabolism....
Barley is recognized as one of the most diverse cereal crops. It can be divided as winter or spri... more Barley is recognized as one of the most diverse cereal crops. It can be divided as winter or spring types by growth habit, two-rowed and six-rowed by spike morphology, hulled or hull-less by hull adherence, and malting or non-malting based on its intended use. In this study, twenty eight barley samples, including covered and naked types, were collected from field trials of the Agricultural Institute Osijek. These samples were subjected to grain analyses, comprising protein content, amylose and β-glucan content. The samples were micro-malted and the malts were analysed to determine differences in quality parameters between different groups of barleys. The most obvious differences found betwwen hulled and hull-less barley types were the malt extract development and cytolytic modification achieved during malting process. Variation in the β-glucan content betweenthe hulled and hull-less samples shows a tendency towards a higher β-glucan content in hull-less barley varieties. Among malti...
Fusarium head blight (FHB) caused by fungi Fusarium spp., is a serious wheat (Triticum aestivum L... more Fusarium head blight (FHB) caused by fungi Fusarium spp., is a serious wheat (Triticum aestivum L.) disease that can reduce yield and quality of the g
Plants, 2021
Genotype-dependent responses of apples to drought stress were evaluated between commercial and tr... more Genotype-dependent responses of apples to drought stress were evaluated between commercial and traditional apple cultivars. The results indicate different mechanisms of tolerance to investigated drought stress conditions. Chlorophyll fluorescence induction (OJIP) parameters, chlorophyll and carotenoid content, malondialdehyde (MDA), hydrogen peroxide (H2O2), proline, phenols and leaf water content (WC) were measured. The traditional cultivar “Crvenka” confirmed the best tolerance to a drought stress condition, presenting higher photosynthetic efficiency, higher leaf water content, higher levels of chlorophyll content and lower lipid peroxidation with greater membrane stability. The commercial cultivar “Golden Delicious Reinders” showed decreased water content in leaves, increased lipid peroxidation levels and photoinhibition. Considering all results, the commercial cultivar “Golden Delicious Reinders” was adversely affected by drought, while traditional cultivars exhibited better to...
Journal of Central European Agriculture, 2020
Kernel water (KW) and total soluble sugars (TSS) are major components of sweet corn taste, while ... more Kernel water (KW) and total soluble sugars (TSS) are major components of sweet corn taste, while total carotenoids (TC), total phenols (TP) and antioxidant activity (AA) contribute to its health benefits. Main objective was to determine the effect of genotype, harvest date and year on chemical components of kernel quality in shrunken (sh2) sweet corn. Nine sh2 genotypes were harvested at 3-day intervals from 20 to 32 days after pollination (DAP) over two years. Growing season significantly affected TC and TP only. Significant differences among genotypes were found for KW (75.3-77.4%), TTS (282.4-343.2 mg/g), TC (9.3-15.7 µg/g), TP (243.6-289.4 mg GEA/100 g) and AA (74.6-83.6 % inhibition). Effect of harvest date and genotype by harvest date interaction was significant for all traits. Compared to the 20 DAP, KW and TSS decreased, while TC, TP and AA tended to increase with later harvest dates. Based on KW, genotypes OS 5Esh and Overland extended harvest window up to 29 DAP. In contrast, optimum harvest window for genotype OS 244sh finished at 20 DAP. Extended harvest window for genotype Overland resulted in significant increase of TC (62.8%), TP (12.1%) and AA (12.4%).
Acta Physiologiae Plantarum, 2020
Yield gains in wheat are a result of greater plant photosynthetic efficiency and better ability t... more Yield gains in wheat are a result of greater plant photosynthetic efficiency and better ability to tolerate biotic or abiotic stresses due to the protection of photosynthetic structures from reactive oxygen species (ROS) by efficient antioxidative system. The slower rate of senescence could be associated to higher grain yield. The objective of this study was to identify antioxidative signalling in two different winter wheat varieties which regulates the process of senescence. Concentration of malondialdehyde (MDA) and hydrogen peroxide (H2O2) content, activities of the antioxidant enzymes (catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GPOD; EC 1.11.1.7) and polyphenol oxidase (PPO; EC 1.14.18.1)), chlorophyll (Chl) and carotenoid (Car) concentration as well as photosynthetic efficiency in the flag leaves were determined. Results showed that compared to high-quality variety (Olimpija), Kraljica (high-yielding variety) increased CAT, APX, GPOD and PPO activities earlier during the course time experiment, which could be the reason of delayed senescence process. Oxidative damage resulting from increased H2O2 accumulation with increased lipid peroxidation and a decline in antioxidative enzymes activity may contribute to accelerated senescence in Olimpija. Furthermore, delay in senescence in Kraljica was associated with a decrease in the grain protein content. As a result of declination in Chl a + b and carotenoid content, MDA concentration in the flag leaves of Olimpija gradually increased at each sampling point with decline in antioxidant enzymes activity. However, the rate of protein and chlorophyll degradation occurs normally below the green tissue where Kraljica maintained higher chlorophyll level longer then Olimpija.
Biologia plantarum, 2020
Wheat (Triticum aestivum L.) is leading cereal crop worldwide, but its yield is highly affected d... more Wheat (Triticum aestivum L.) is leading cereal crop worldwide, but its yield is highly affected due to various diseases, especially Fusarium head blight (FHB), which affects the metabolism of plants. The present study was conducted at the Agricultural Institute Osijek using three winter wheat cultivars (Apache, Bezostaya1, and U1) during 2016/2017. The objectives of our studies were to examine differences in physiological characteristics of FHB resistance among wheat cultivars in the early stage of infection. The FHB incidence and severity was the highest in 'Bezostaya1'. Results suggest that activation of some anti-oxidative enzymes in the first 2 h after Fusarium attack was not efficient to prevent disease. 'Apache', which revealed an average FHB incidence, efficiently activated defence response through phenol metabolism elevation. The most effective defence response trough activation of anti-oxidative enzymes triggered by H2O2 was revealed in 'U1', which resulted in a minimal FHB incidence and disease severity. The obtained results confirm differences in defence strategies of wheat genotypes.
Theoretical and Experimental Plant Physiology, 2019
Glasnik zaštite bilja, 2019
Posljednjih deset godina svjedoci smo klimatskih promjena uslijed djelovanja različitih štetnih u... more Posljednjih deset godina svjedoci smo klimatskih promjena uslijed djelovanja različitih štetnih utjecaja na okoliš. U poljoprivrednoj proizvodnji rješenja se sve više traže u ekološkoj proizvodnji koja se temelji na korištenju metoda koje ne ometaju prirodne zakone te ne djeluju štetno na agroekološki sustav kao ni na zdravlje ljudi. S obzirom na velike troškove sadnje novog sortimenta prilikom prelaska na ekološku proizvodnju, cilj ovog istraživanja je bio utvrditi osjetljivost standardnih sorti na glavnu bolest jabuke, mrljavost lista i krastavost ploda (Venturia inaequalis), u sustavu ekološke zaštite u usporedbi s kontrolom tj. ne tretiranim djelom pokusa. Drugi cilj je bio utvrditi učinkovitost ekološkog tretmana na otpornom ili tolerantnom sortimentu jabuke. Dvogodišnji rezultati istraživanja pokazali su da su sorte 'Elshof' i…
Acta Biologica Hungarica, 2018
In wheat, Fusarium fungus promotes the appearance of destructive disease named as Fusarium head b... more In wheat, Fusarium fungus promotes the appearance of destructive disease named as Fusarium head blight (FHB) that can cause grain yield reduction and mycotoxin accumulation. The focus of this research was to verify the influence of Fusarium graminearum and F. culmorum on wheat genotypes with different susceptibility to FHB: "Super Žitarka" (susceptible), "Lucija" (moderately resistant) and "Apache" (resistant). The experiment was performed under field conditions by artificial spore inoculation of ears at the flowering stage. The effectiveness of antioxidative enzymes, hydrogen peroxide (H 2 O 2) content and malondialdehyde (MDA) content were observed at several sampling points after Fusarium inoculation (3, 15 and 24 hours). "Lucija" responded to pathogen by increase of guaiacol peroxidase (POD) activity, high H 2 O 2 and MDA content in the early post-inoculation times (3 and 15 hours), compared to control. "Super Žitarka" displayed inhibition of catalase (CAT) activity throughout the whole time course of the experiment. Infected plants of "Apache" showed notable decline in MDA content over time. Moreover, in "Apache" increased H 2 O 2 accumulation was observed immediately after Fusarium exposure (3 and 15 hours), compared to 24 hours. Rapid overproduction of H 2 O 2 under Fusarium stress marked "Apache" as FHB-resistant.
Poljoprivreda, 2016
The aim of this research was to investigate the variability of photosynthetic performance index (... more The aim of this research was to investigate the variability of photosynthetic performance index (PI ABS) and leaf temperature values measured in V6 development phase on 13 sunflower hybrids, grown in stressful conditions. The pot trial was made up of two treatments, one (T1) with 60% Field Water Capacity (FWC), and the other one (T2) with 80% FWC. Significant differences between T1 and T2 treatments were established for both of these parameters which prove their dependence on the water content in the soil, while the influence of hybrid was evident only in the case of PI ABS. Although in T1, as opposed to T2, all sunflower hybrids reacted by increasing leaf temperature, reaction to stress conditions measured with PI ABS parameter was not uniform. Some of the hybrids reacted by decreasing PI ABS values, while others reacted by increasing their PI ABS values. Therefore, it can be concluded that changes in parameters were independent of each other, which was confirmed by correlation analysis. Investigated parameters are suitable for determining the existence of undesirable environmental conditions that cause stress in plants and can be used in breeding of sunflower to withstand abiotic stress conditions, i.e. in selection of stress tolerant hybrids.
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Papers by Marija Viljevac Vuletić