The effect of cut style and placental pollination on fertilization efficiency was studied for com... more The effect of cut style and placental pollination on fertilization efficiency was studied for compatible Aechmea fasciata plants. These alternative in vitro pollination techniques resulted in lower penetration rates of the ovules by pollen tubes in comparison to pollination on the stigma. An explanation was found in the intervention of the normal pollination process,through which less pollen-pistil interactions were built
In order to save manual labour in plant tissue propagation, we tried adding liquid media to estab... more In order to save manual labour in plant tissue propagation, we tried adding liquid media to established, exhausted cultures, instead of transplanting the tissues to a fresh medium. This has been investigated for the elongation and for root induction stages with Cordyline, Philodendron, Magnolia and Spathiphyllum. The salt concentration and the application time are important factors, determining the success of this method. Charcoal added to the liquid stage Ilia medium eliminated the residual effects of BA applied during stage II.
... LaRue, TAG & Gamborg, OL 1971: Ethylene produc-tion by plant cell cultures. Variations in... more ... LaRue, TAG & Gamborg, OL 1971: Ethylene produc-tion by plant cell cultures. Variations in production during growing cycle and in different plant species. - Plant Physiol. ... Annu. Rev. Plant Physiol. ... Mackenzie, IA & Street, HE 1970. Studies on the growth in culture of plant cells. ...
Pollen germination on stigma, pollen tube growth in the style and pollen tube penetration of ovul... more Pollen germination on stigma, pollen tube growth in the style and pollen tube penetration of ovules have been studied of excised pistils, after crossing and selfing of 7 bromeliad species. Prefertilization barriers were observed after intraspecific, interspecific and intergeneric crosses. Satisfactory pollen germination in both selfed and crossed flowers showed that the stigma did not act as a barrier. Self-incompatibility in Aechmea B and Tillandsia A was confined in the style, which is characteristic for the gametophytic SI system. Unilateral incongruity between Aechmea A and Aechmea B was not related to self-incompatibility, but could be explained by differences in style length. Incompatibility, interspecific and intergeneric incongruity were different with respect to the number of pollen tubes in the style, ratio of pollen tube length and style length, and the number of pollen tube abnormalities. The prefertilization barriers of intergeneric crosses between different subfamilies were not greater than these observed within a subfamily.
The effect of cut style and placental pollination on fertilization efficiency was studied for com... more The effect of cut style and placental pollination on fertilization efficiency was studied for compatible Aechmea fasciata plants. These alternative in vitro pollination techniques resulted in lower penetration rates of the ovules by pollen tubes in comparison to pollination on the stigma. An explanation was found in the intervention of the normal pollination process,through which less pollen-pistil interactions were built
In order to save manual labour in plant tissue propagation, we tried adding liquid media to estab... more In order to save manual labour in plant tissue propagation, we tried adding liquid media to established, exhausted cultures, instead of transplanting the tissues to a fresh medium. This has been investigated for the elongation and for root induction stages with Cordyline, Philodendron, Magnolia and Spathiphyllum. The salt concentration and the application time are important factors, determining the success of this method. Charcoal added to the liquid stage Ilia medium eliminated the residual effects of BA applied during stage II.
... LaRue, TAG & Gamborg, OL 1971: Ethylene produc-tion by plant cell cultures. Variations in... more ... LaRue, TAG & Gamborg, OL 1971: Ethylene produc-tion by plant cell cultures. Variations in production during growing cycle and in different plant species. - Plant Physiol. ... Annu. Rev. Plant Physiol. ... Mackenzie, IA & Street, HE 1970. Studies on the growth in culture of plant cells. ...
Pollen germination on stigma, pollen tube growth in the style and pollen tube penetration of ovul... more Pollen germination on stigma, pollen tube growth in the style and pollen tube penetration of ovules have been studied of excised pistils, after crossing and selfing of 7 bromeliad species. Prefertilization barriers were observed after intraspecific, interspecific and intergeneric crosses. Satisfactory pollen germination in both selfed and crossed flowers showed that the stigma did not act as a barrier. Self-incompatibility in Aechmea B and Tillandsia A was confined in the style, which is characteristic for the gametophytic SI system. Unilateral incongruity between Aechmea A and Aechmea B was not related to self-incompatibility, but could be explained by differences in style length. Incompatibility, interspecific and intergeneric incongruity were different with respect to the number of pollen tubes in the style, ratio of pollen tube length and style length, and the number of pollen tube abnormalities. The prefertilization barriers of intergeneric crosses between different subfamilies were not greater than these observed within a subfamily.
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