The need for rice continues to increase along with the increase in population. Efforts to increas... more The need for rice continues to increase along with the increase in population. Efforts to increase rice production is generally carried out through proper and balanced fertilization. Other than that, plant growth-promoting actinobacterial (PGPB) inoculants can be used as an alternative solution. This study aimed to examine the effect of the application of actinobacterial seed dressing and NPK fertilizers on the growth of rice plants grown in a glass house. A randomized block design with three factors was conducted, consisting of actinobacterial seed dressing (added and not added), type of carrier (zeolite, peat, and combinations) and doses of NPK fertilizer (0 g/pot, 0.375 g/pot, and 0.75 g/pot). The addition of actinobacteria consortium, peat-zeolite combination 1:3, and NPK fertilizer at a dose of 0.75 g/pot (A2C3P3) gave consistent results in increasing the average yield of rice vegetative and reproductive parameters observed in the glass house. The A2C3P3 treatment had a significant effect on the number of tillers, width of flag leaf, dry weight of roots and shoots of rice observed at 10 WAP compared to other treatment combinations. The actinobacterial seed coating plays a pivotal role in supporting rice plant growth.
The further nodulation, nodule development and nitrogen fixation by nodulated Medicago polymorpha... more The further nodulation, nodule development and nitrogen fixation by nodulated Medicago polymorpha and M. truncatula plants transferred from pH 7.0 to three lower pH conditions (4.5, 5.0 and 5.5) in hydroponic culture was examined. Under these conditions, further nodule initiation by strains CC169 and WSM540 was severely retarded. Nodule development (mg nodule dry weight plant-1) was similar on plants at pH 5.0 and 5.5 to that on plants kept at pH 7.0, but at pH 4.5, it was less. Under the lower pH conditions, the nodules were 2–3 times the mass of those found at pH 7.0. Nitrogen fixation, expressed as µg N2 mg-1 (nodule dry weight) d-1, was largely unaffected by pH, and the apparently lower levels of nitrogen fixation at pH 4.5 could be attributed to an effect on nodule development. The determination of nitrogenase activity by acetylene reduction assay showed a strong influence of pH on the observed rates (per plant, or on a mg nodule dry weight basis). No explanation can be offered for this anomoly, but it does indicate the need for caution in applying the assay in this type of experimentation. The four host x strain combinations showed a similar response to the lower pH conditions, despite the fact that M. polymorha fixed higher levels of nitrogen, at a higher rate per mg nodule dry weight, than M. truncatula.
Endophytic actinobacteria can generate bioactive compounds similar to those produced by the host ... more Endophytic actinobacteria can generate bioactive compounds similar to those produced by the host plant. The Eleutherine palmifolia (L.) Merr is an example of a widely utilized host plant in the Dayak community. Flavonoids, which have antioxidant effects, are among the bioactive compounds found in E. palmifolia. The antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia remains unidentified. The aim of this study was to examine the antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia. Antioxidant activity from isolates actinobacteria was determined quantitatively using the DPPH and ABTS methods. Identification of the types of bioactive compound in the samples was identified using TLC assay and bioautography test. Analysis of flavonoid levels was performed using colorimetric aluminum chloride (AlCl3). The result showed that the extraction of six isolates of endophytic actinobacteria with ethyl acetate solvent had different yields. The extract of endophytic actinobacteria EPB 6 isolate produced the highest (0.013%) yield. The extract of endophytic actinobacteria EPB 3 isolate had the best antioxidant activity, with 53.85 mg/mL (DPPH) and 42.43 mg/mL (ABTS). The extract of endophytic actinobacteria EPB 3 isolate and E. palmifolia had the same blue spots in TLC analysis, indicating the presence of bioactive compounds flavonol and flavones groups. The bioautography test of EPB 3 isolate endophytic actinobacteria extract produced yellow color, indicating qualitatively antioxidant activity. The total flavonoid content of the EPB 3 isolate extract was also higher (25.30 QE/mg) than the other five isolates, while it was 46.03 QE/mg in E. palmifolia extract. It is clear from the present study that the endophytic actinobacteria extract can produce antioxidants.
Global climate change will result in decreased rainfall and increased evaporation. Thus, it is es... more Global climate change will result in decreased rainfall and increased evaporation. Thus, it is estimated that the frequency and severity of drought stress will get worse. Silica increases plant drought resistance by improving water use efficiency in plants. Despite its abundant availability in soil, most silica sources are not available to plants due to their low solubility. Silica solubilizing bacteria (SSB) have an important role in increasing the available silica. This study aims to observe the silica solubilizing activity of three SSB isolates collections of PPBBI on insoluble silica sources, including magnesium trisilicate, quartz, and feldspar, and see their effects on increasing water use efficiency in corn plants via drought experiments. SSB activity was measured using the modified standard method of 4500-SiO2 D Heteropoly blue. Drought control in the greenhouse follows the Snow and Tingey system. The experimental design used a completely randomized design factorial with irr...
4 Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Pertanian Bogor, Dr... more 4 Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Pertanian Bogor, Dramaga Campus, Bogor 16680, Indonesia; Research Center for Biology, Indonesian Institute of Sciences, Cibinong Science Center,Cibinong 16911, Indonesia; Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong Science Center, Cibinong 16911, Indonesia; Biopharmaca Research Center, Institut Pertanian Bogor, Bogor 16151, Indonesia
Article history: Received 25 June 2014 Received in revised form 8 July 2014 Accepted 14 September... more Article history: Received 25 June 2014 Received in revised form 8 July 2014 Accepted 14 September 2014 Available online 10 October 2014
Biosaintifika: Journal of Biology & Biology Education, Dec 31, 2017
Rhododendron has been known to treat various diseases including diarrhea, but diversity and poten... more Rhododendron has been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria have not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assessed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in the ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escherichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twentythree of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e., RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 o C. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/ mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contributes to the understanding of their diversity and potency as an antibacterial agent.
A total of 2 actinomycetes were isolated 29 strains of and identified by full sequence of 16S rRN... more A total of 2 actinomycetes were isolated 29 strains of and identified by full sequence of 16S rRNA gene analysis. Samples ed-from , Bali Island, consist of 18 soil and 20 leaf litter were collected Eka Karya Botanical Garden Indonesia. Two isolation methods, SDS-east xtract (SY) and Rehydration-Centrifugation (RC) used. i.e. Y E were in this study Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 2 genera. B 8 ased on molecular analysis of 16S rRNA gene similarities showed that Eka Karya Botanical Garden. isolated actinomycetes of origin diverse is Analysis on from soil samples, resulted in is most 144 isolates 24 genera and more than 87 species. the Streptomyces dominant gen where or from isolated actinomycetes belong to this genus. It was followed by us 65 isolates 45% Actinoplanes (25 isolates = 17%). From leaf-litter 85 isolates 9 genera samples, the total number of may be group into ed and more than 41 species. The most dominated genus is (42 isolates = 49%) (16 Actinoplanes Catenuloplanes followed by isolates = 19%).
The diversity of the actinomycete community associated with Neofibularia sp. from Bira Island, In... more The diversity of the actinomycete community associated with Neofibularia sp. from Bira Island, Indonesia, has been largely unstudied. This study was undertaken to address the paucity of information in this respect. Methodology and results: Culturable actinomycetes were isolated and cultured on HV medium. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to analyse the structure of the actinomycete community. Five culturable actinomycetes that were isolated belonged to the genus Streptomyces. They showed various degrees of similarity to the reference strains Streptomyces sampsonii (97-99%), Streptomyces resistomycificus (97-99%), Streptomyces gougerotii (97-99%), Streptomyces erringtonii (97-99%), and Streptomyces albus (97-99%). The culturable actinomycetes isolates also showed differences in morphological characteristics as compared with the reference strains. The metagenomic analysis suggested that the actinomycete community was dominated by rare actinomycetes. Eight DGGE DNA bands that were obtained had sequences that showed similarities to Ferrithrix thermotolerans (88-94%), Lamia majanohamensis (87-92%), Aciditerrimonas ferrireducens (87-92%), and Thermobispora bispora (85-92%), while 4 bands had sequences similar to Propionibacterium acnes (97-100%) and another band matched sequences belonging to an uncultured bacterium clone (86-87%). The actinomycetes detected by the metagenomic approach were assigned identities that were mostly under 97.5% as compared with reference strains available in Genbank. Conclusion, significance and impact of study: Observations from both culture and DGGE analysis give a better understanding of the diversity and community structure of actinomycetes associated with Neofibularia sp. The culturable actinomycetes were Streptomyces spp., while rare actinomycetes were dominant when the metagenomic approach was adopted. Several of these actinomycetes showed identities below 97% when matched to reference strains, indicating possible novel species associated with the sponge Neofibularia.
International Journal of Pharmacy and Pharmaceutical Sciences, Apr 28, 2015
Objective: This research work aimed to assess the capability of endophytic Streptomyces sp. IPBCC... more Objective: This research work aimed to assess the capability of endophytic Streptomyces sp. IPBCC. b.15.1539 isolated from Tinospora crispa inproducing α-glucosidase inhibitor compound and examined the effect of its ethyl acetate extract containing α glucosidase inhibitor in lowering blood glucose in streptozotozin mice. Methods: Streptomyces sp. IPBCC. b.15.1539 was grown in a bioreactor filled with International Streptomyces Project 2 medium, for 5, 10, 15, and 20 days, and assayed for its in vitro α-glucosidase inhibitory activity. The ethyl acetate extract was examined for its IC50 Results: The crude extract produced value. An in vivo experiment was set up using thirty mice, which were divided into five treatment groups: (a) acarbose (0.03 mg/30 g body weight) used as a positive control, (b) placebo used as a negative control, (c-e) treatment groups were treated with ethyl acetate extract at 0.036 mg/30 g body weight (P1), 0.36 mg/30 gbody weight (P2), 0.036 mg/30 g body weight (P3). 98.5% α-glucosidase inhibitory activity, with 15,6 biomass after 10 days of production. The ethyl acetateextract at a concentration of 1000 µg/ml produced 96.08% α-glucosidase inhibitory activity, while acarbose at the same concentration gave 97.46% inhibition. The IC50 for the ethyl acetate extract Conclusions: The was 0.047 µg/ml, while for acarbose was 0.003 µg/ml. The ethyl acetate extract applied as the P1 treatment group lowered blood glucose levels in streptozotozin mice by 26%. α-glucosidase inhibitor of Streptomyces sp. IPBCC. b.15.1539 of T. crispa has the potency as an antidiabetic agent for type 2 DM therapy.
Six isolates of Streptomyces spp. producing antibacterial compounds isolated from soils in Sukabu... more Six isolates of Streptomyces spp. producing antibacterial compounds isolated from soils in Sukabumi, Kepulauan Seribu, Cipanas, and East Kalimantan were identified based on their morphology, cell wall chemotype and 16S rDNA sequence analysis. These isolates produced various growth inhibition responses against Gram-positive Bacillus subtilis and Gram-negative Xanthomonas axonopodis, with spore chain morphology spiral and hooked to looped, and LL-DAP type cell wall. LC17-2 (from Lancang, Kepulauan Seribu) showed 98% similarity with Streptomyces thermodiastaticus JCM4840, while B56-2 (from Bokor, Kepulauan Seribu) showed the closest similarity (99%) with S. padanus PMS-702 and type strain ATCC 25646. We have no blast result from PS4-16 because we could not isolate its DNA. Meanwhile, strain SLW8-1 (from Sukabumi) had the closest similarity (98%) with S. cebimarensis DSM 41798T. Both IVNF1-1 (from Kutai-East Kalimantan) and PS1-4 (from Pasir Sarongge Cipanas) gave the closest similarity (99%) with S. lavendulae IFO14028. Key words: Streptomyces spp., antibacterial activity, morphology, cell wall chemotype, 16S rDNA analysis
Indigenous actinomycetes are known to have high biodiversity and chance to acquire a novel specie... more Indigenous actinomycetes are known to have high biodiversity and chance to acquire a novel species. Molecular identification and the role of rice endophytic actinomycetes need to be studied. The research aimed to identify endophytic actinomycetes from Indonesian rice plant based on 16S rRNA and nifH genes properties. DNA genome from the seven isolates of endophytic actinomycetes was isolated using Genomic DNA Mini Kit followed by PCR amplification of 16S rRNA and nifH genes. Indication of their nitrogen fixing activities was conducted based on their capability to grow in Nfree medium, ammonia production, and presence of nifH gene. PCR products were sequenced and analyzed by bioinformatics software to construct phylogenetic tree. An analysis of 16S rRNA gene sequences demonstrated that the seven isolates are most closely related to Streptomyces spp. The 16S rRNA gene sequences of the six isolates were closed related with S. albolongus, S. cavourensis subsp. cavourensis, S. anulatus, ...
Biological role of endophytic Streptomyces spp. which lives inside rice plant tissue may promote ... more Biological role of endophytic Streptomyces spp. which lives inside rice plant tissue may promote rice plant growth. The research aimed to examine the capability of rice endophytic Streptomyces spp. in producing indole acetic acid (IAA) and fixing nitrogen. In vitro production of IAA from ten selected endophytic Streptomyces spp. isolated from local rice varieties and agricultural soil was examined. The tested isolates were grown in a liquid medium of International Streptomyces Project (ISP) no. 2 with the addition of 0.2 ml 0.2% L-tryptophan. All tested isolates produced indole acetic acid with various concentrations ranging about 11-99 ppm. Three isolates which capable of producing high indole acetic acid were AB131-1 (99.2 ppm), AB131-2 (99.2 ppm), and PS4-16 (93.4 ppm). Meanwhile, based on in-vitro assay, the tested isolates have the ability to grow in N-free medium, able to reduce acetylene and produced ammonium with various activity. The SSW-02 was considered as the most promis...
Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early develop... more Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages. Biodiversitas 11 (2): 65-68.Terminal Restriction Fragment Length Polymorphism (T-RFLP) was used to monitor the dynamics of the bacterial communities associated with early developmental stages of white shrimp (Litopenaeus vannamei) larvae. Samples for analysis were egg, hatching nauplii, 24 hours old nauplii, and 48 hours old nauplii which were collected from one cycle of production at commercial hatchery. T-RFLP results indicated that the bacterial community associated with early stages of shrimp development might be transferred vertically from broodstock via egg. There was no significant difference between bacterial communities investigated, except the bacterial community of 48 hours old nauplii. Diversity analyses showed that the bacterial community of egg had the highest diversity and evenness, meanwhile the bacterial community of 48 hours old nauplii had the lowest diversity. Nine phylotypes were found at all stages with high abundance. Those TRFs were identified as γ-proteobacteria, α-proteobacteria, and bacteroidetes group.
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The need for rice continues to increase along with the increase in population. Efforts to increas... more The need for rice continues to increase along with the increase in population. Efforts to increase rice production is generally carried out through proper and balanced fertilization. Other than that, plant growth-promoting actinobacterial (PGPB) inoculants can be used as an alternative solution. This study aimed to examine the effect of the application of actinobacterial seed dressing and NPK fertilizers on the growth of rice plants grown in a glass house. A randomized block design with three factors was conducted, consisting of actinobacterial seed dressing (added and not added), type of carrier (zeolite, peat, and combinations) and doses of NPK fertilizer (0 g/pot, 0.375 g/pot, and 0.75 g/pot). The addition of actinobacteria consortium, peat-zeolite combination 1:3, and NPK fertilizer at a dose of 0.75 g/pot (A2C3P3) gave consistent results in increasing the average yield of rice vegetative and reproductive parameters observed in the glass house. The A2C3P3 treatment had a significant effect on the number of tillers, width of flag leaf, dry weight of roots and shoots of rice observed at 10 WAP compared to other treatment combinations. The actinobacterial seed coating plays a pivotal role in supporting rice plant growth.
The further nodulation, nodule development and nitrogen fixation by nodulated Medicago polymorpha... more The further nodulation, nodule development and nitrogen fixation by nodulated Medicago polymorpha and M. truncatula plants transferred from pH 7.0 to three lower pH conditions (4.5, 5.0 and 5.5) in hydroponic culture was examined. Under these conditions, further nodule initiation by strains CC169 and WSM540 was severely retarded. Nodule development (mg nodule dry weight plant-1) was similar on plants at pH 5.0 and 5.5 to that on plants kept at pH 7.0, but at pH 4.5, it was less. Under the lower pH conditions, the nodules were 2–3 times the mass of those found at pH 7.0. Nitrogen fixation, expressed as µg N2 mg-1 (nodule dry weight) d-1, was largely unaffected by pH, and the apparently lower levels of nitrogen fixation at pH 4.5 could be attributed to an effect on nodule development. The determination of nitrogenase activity by acetylene reduction assay showed a strong influence of pH on the observed rates (per plant, or on a mg nodule dry weight basis). No explanation can be offered for this anomoly, but it does indicate the need for caution in applying the assay in this type of experimentation. The four host x strain combinations showed a similar response to the lower pH conditions, despite the fact that M. polymorha fixed higher levels of nitrogen, at a higher rate per mg nodule dry weight, than M. truncatula.
Endophytic actinobacteria can generate bioactive compounds similar to those produced by the host ... more Endophytic actinobacteria can generate bioactive compounds similar to those produced by the host plant. The Eleutherine palmifolia (L.) Merr is an example of a widely utilized host plant in the Dayak community. Flavonoids, which have antioxidant effects, are among the bioactive compounds found in E. palmifolia. The antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia remains unidentified. The aim of this study was to examine the antioxidant activity of bioactive compounds produced by endophytic actinobacteria of E. palmifolia. Antioxidant activity from isolates actinobacteria was determined quantitatively using the DPPH and ABTS methods. Identification of the types of bioactive compound in the samples was identified using TLC assay and bioautography test. Analysis of flavonoid levels was performed using colorimetric aluminum chloride (AlCl3). The result showed that the extraction of six isolates of endophytic actinobacteria with ethyl acetate solvent had different yields. The extract of endophytic actinobacteria EPB 6 isolate produced the highest (0.013%) yield. The extract of endophytic actinobacteria EPB 3 isolate had the best antioxidant activity, with 53.85 mg/mL (DPPH) and 42.43 mg/mL (ABTS). The extract of endophytic actinobacteria EPB 3 isolate and E. palmifolia had the same blue spots in TLC analysis, indicating the presence of bioactive compounds flavonol and flavones groups. The bioautography test of EPB 3 isolate endophytic actinobacteria extract produced yellow color, indicating qualitatively antioxidant activity. The total flavonoid content of the EPB 3 isolate extract was also higher (25.30 QE/mg) than the other five isolates, while it was 46.03 QE/mg in E. palmifolia extract. It is clear from the present study that the endophytic actinobacteria extract can produce antioxidants.
Global climate change will result in decreased rainfall and increased evaporation. Thus, it is es... more Global climate change will result in decreased rainfall and increased evaporation. Thus, it is estimated that the frequency and severity of drought stress will get worse. Silica increases plant drought resistance by improving water use efficiency in plants. Despite its abundant availability in soil, most silica sources are not available to plants due to their low solubility. Silica solubilizing bacteria (SSB) have an important role in increasing the available silica. This study aims to observe the silica solubilizing activity of three SSB isolates collections of PPBBI on insoluble silica sources, including magnesium trisilicate, quartz, and feldspar, and see their effects on increasing water use efficiency in corn plants via drought experiments. SSB activity was measured using the modified standard method of 4500-SiO2 D Heteropoly blue. Drought control in the greenhouse follows the Snow and Tingey system. The experimental design used a completely randomized design factorial with irr...
4 Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Pertanian Bogor, Dr... more 4 Department of Biology, Faculty of Mathematic and Natural Sciences, Institut Pertanian Bogor, Dramaga Campus, Bogor 16680, Indonesia; Research Center for Biology, Indonesian Institute of Sciences, Cibinong Science Center,Cibinong 16911, Indonesia; Research Center for Biotechnology, Indonesian Institute of Sciences, Cibinong Science Center, Cibinong 16911, Indonesia; Biopharmaca Research Center, Institut Pertanian Bogor, Bogor 16151, Indonesia
Article history: Received 25 June 2014 Received in revised form 8 July 2014 Accepted 14 September... more Article history: Received 25 June 2014 Received in revised form 8 July 2014 Accepted 14 September 2014 Available online 10 October 2014
Biosaintifika: Journal of Biology & Biology Education, Dec 31, 2017
Rhododendron has been known to treat various diseases including diarrhea, but diversity and poten... more Rhododendron has been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria have not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assessed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in the ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escherichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twentythree of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e., RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 o C. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/ mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contributes to the understanding of their diversity and potency as an antibacterial agent.
A total of 2 actinomycetes were isolated 29 strains of and identified by full sequence of 16S rRN... more A total of 2 actinomycetes were isolated 29 strains of and identified by full sequence of 16S rRNA gene analysis. Samples ed-from , Bali Island, consist of 18 soil and 20 leaf litter were collected Eka Karya Botanical Garden Indonesia. Two isolation methods, SDS-east xtract (SY) and Rehydration-Centrifugation (RC) used. i.e. Y E were in this study Based on 16S rRNA gene analysis, isolated actinomycetes may be grouped into 2 genera. B 8 ased on molecular analysis of 16S rRNA gene similarities showed that Eka Karya Botanical Garden. isolated actinomycetes of origin diverse is Analysis on from soil samples, resulted in is most 144 isolates 24 genera and more than 87 species. the Streptomyces dominant gen where or from isolated actinomycetes belong to this genus. It was followed by us 65 isolates 45% Actinoplanes (25 isolates = 17%). From leaf-litter 85 isolates 9 genera samples, the total number of may be group into ed and more than 41 species. The most dominated genus is (42 isolates = 49%) (16 Actinoplanes Catenuloplanes followed by isolates = 19%).
The diversity of the actinomycete community associated with Neofibularia sp. from Bira Island, In... more The diversity of the actinomycete community associated with Neofibularia sp. from Bira Island, Indonesia, has been largely unstudied. This study was undertaken to address the paucity of information in this respect. Methodology and results: Culturable actinomycetes were isolated and cultured on HV medium. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to analyse the structure of the actinomycete community. Five culturable actinomycetes that were isolated belonged to the genus Streptomyces. They showed various degrees of similarity to the reference strains Streptomyces sampsonii (97-99%), Streptomyces resistomycificus (97-99%), Streptomyces gougerotii (97-99%), Streptomyces erringtonii (97-99%), and Streptomyces albus (97-99%). The culturable actinomycetes isolates also showed differences in morphological characteristics as compared with the reference strains. The metagenomic analysis suggested that the actinomycete community was dominated by rare actinomycetes. Eight DGGE DNA bands that were obtained had sequences that showed similarities to Ferrithrix thermotolerans (88-94%), Lamia majanohamensis (87-92%), Aciditerrimonas ferrireducens (87-92%), and Thermobispora bispora (85-92%), while 4 bands had sequences similar to Propionibacterium acnes (97-100%) and another band matched sequences belonging to an uncultured bacterium clone (86-87%). The actinomycetes detected by the metagenomic approach were assigned identities that were mostly under 97.5% as compared with reference strains available in Genbank. Conclusion, significance and impact of study: Observations from both culture and DGGE analysis give a better understanding of the diversity and community structure of actinomycetes associated with Neofibularia sp. The culturable actinomycetes were Streptomyces spp., while rare actinomycetes were dominant when the metagenomic approach was adopted. Several of these actinomycetes showed identities below 97% when matched to reference strains, indicating possible novel species associated with the sponge Neofibularia.
International Journal of Pharmacy and Pharmaceutical Sciences, Apr 28, 2015
Objective: This research work aimed to assess the capability of endophytic Streptomyces sp. IPBCC... more Objective: This research work aimed to assess the capability of endophytic Streptomyces sp. IPBCC. b.15.1539 isolated from Tinospora crispa inproducing α-glucosidase inhibitor compound and examined the effect of its ethyl acetate extract containing α glucosidase inhibitor in lowering blood glucose in streptozotozin mice. Methods: Streptomyces sp. IPBCC. b.15.1539 was grown in a bioreactor filled with International Streptomyces Project 2 medium, for 5, 10, 15, and 20 days, and assayed for its in vitro α-glucosidase inhibitory activity. The ethyl acetate extract was examined for its IC50 Results: The crude extract produced value. An in vivo experiment was set up using thirty mice, which were divided into five treatment groups: (a) acarbose (0.03 mg/30 g body weight) used as a positive control, (b) placebo used as a negative control, (c-e) treatment groups were treated with ethyl acetate extract at 0.036 mg/30 g body weight (P1), 0.36 mg/30 gbody weight (P2), 0.036 mg/30 g body weight (P3). 98.5% α-glucosidase inhibitory activity, with 15,6 biomass after 10 days of production. The ethyl acetateextract at a concentration of 1000 µg/ml produced 96.08% α-glucosidase inhibitory activity, while acarbose at the same concentration gave 97.46% inhibition. The IC50 for the ethyl acetate extract Conclusions: The was 0.047 µg/ml, while for acarbose was 0.003 µg/ml. The ethyl acetate extract applied as the P1 treatment group lowered blood glucose levels in streptozotozin mice by 26%. α-glucosidase inhibitor of Streptomyces sp. IPBCC. b.15.1539 of T. crispa has the potency as an antidiabetic agent for type 2 DM therapy.
Six isolates of Streptomyces spp. producing antibacterial compounds isolated from soils in Sukabu... more Six isolates of Streptomyces spp. producing antibacterial compounds isolated from soils in Sukabumi, Kepulauan Seribu, Cipanas, and East Kalimantan were identified based on their morphology, cell wall chemotype and 16S rDNA sequence analysis. These isolates produced various growth inhibition responses against Gram-positive Bacillus subtilis and Gram-negative Xanthomonas axonopodis, with spore chain morphology spiral and hooked to looped, and LL-DAP type cell wall. LC17-2 (from Lancang, Kepulauan Seribu) showed 98% similarity with Streptomyces thermodiastaticus JCM4840, while B56-2 (from Bokor, Kepulauan Seribu) showed the closest similarity (99%) with S. padanus PMS-702 and type strain ATCC 25646. We have no blast result from PS4-16 because we could not isolate its DNA. Meanwhile, strain SLW8-1 (from Sukabumi) had the closest similarity (98%) with S. cebimarensis DSM 41798T. Both IVNF1-1 (from Kutai-East Kalimantan) and PS1-4 (from Pasir Sarongge Cipanas) gave the closest similarity (99%) with S. lavendulae IFO14028. Key words: Streptomyces spp., antibacterial activity, morphology, cell wall chemotype, 16S rDNA analysis
Indigenous actinomycetes are known to have high biodiversity and chance to acquire a novel specie... more Indigenous actinomycetes are known to have high biodiversity and chance to acquire a novel species. Molecular identification and the role of rice endophytic actinomycetes need to be studied. The research aimed to identify endophytic actinomycetes from Indonesian rice plant based on 16S rRNA and nifH genes properties. DNA genome from the seven isolates of endophytic actinomycetes was isolated using Genomic DNA Mini Kit followed by PCR amplification of 16S rRNA and nifH genes. Indication of their nitrogen fixing activities was conducted based on their capability to grow in Nfree medium, ammonia production, and presence of nifH gene. PCR products were sequenced and analyzed by bioinformatics software to construct phylogenetic tree. An analysis of 16S rRNA gene sequences demonstrated that the seven isolates are most closely related to Streptomyces spp. The 16S rRNA gene sequences of the six isolates were closed related with S. albolongus, S. cavourensis subsp. cavourensis, S. anulatus, ...
Biological role of endophytic Streptomyces spp. which lives inside rice plant tissue may promote ... more Biological role of endophytic Streptomyces spp. which lives inside rice plant tissue may promote rice plant growth. The research aimed to examine the capability of rice endophytic Streptomyces spp. in producing indole acetic acid (IAA) and fixing nitrogen. In vitro production of IAA from ten selected endophytic Streptomyces spp. isolated from local rice varieties and agricultural soil was examined. The tested isolates were grown in a liquid medium of International Streptomyces Project (ISP) no. 2 with the addition of 0.2 ml 0.2% L-tryptophan. All tested isolates produced indole acetic acid with various concentrations ranging about 11-99 ppm. Three isolates which capable of producing high indole acetic acid were AB131-1 (99.2 ppm), AB131-2 (99.2 ppm), and PS4-16 (93.4 ppm). Meanwhile, based on in-vitro assay, the tested isolates have the ability to grow in N-free medium, able to reduce acetylene and produced ammonium with various activity. The SSW-02 was considered as the most promis...
Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early develop... more Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages. Biodiversitas 11 (2): 65-68.Terminal Restriction Fragment Length Polymorphism (T-RFLP) was used to monitor the dynamics of the bacterial communities associated with early developmental stages of white shrimp (Litopenaeus vannamei) larvae. Samples for analysis were egg, hatching nauplii, 24 hours old nauplii, and 48 hours old nauplii which were collected from one cycle of production at commercial hatchery. T-RFLP results indicated that the bacterial community associated with early stages of shrimp development might be transferred vertically from broodstock via egg. There was no significant difference between bacterial communities investigated, except the bacterial community of 48 hours old nauplii. Diversity analyses showed that the bacterial community of egg had the highest diversity and evenness, meanwhile the bacterial community of 48 hours old nauplii had the lowest diversity. Nine phylotypes were found at all stages with high abundance. Those TRFs were identified as γ-proteobacteria, α-proteobacteria, and bacteroidetes group.
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