The gene encoding the recombinant peptide has been synthesized by chemical method and it was suce... more The gene encoding the recombinant peptide has been synthesized by chemical method and it was sucessfully expressed in E. coli . The new method was developed for detection of the antimicrobial activities of the pure recombinant peptide which after has been purified on affinity chromatography and cleaved by the factor Xa.
Antibodies are immunoglobulin molecules which originally are glycoprotein that play role to ident... more Antibodies are immunoglobulin molecules which originally are glycoprotein that play role to identify and neutralize foreign agents such as viruses or bacteria. In this study, antibody protein recombine specificity of E. coli O157:H7 which was purified using hybrid methods. Next, antibody activity was tested by the methods as Dot Blot, Western Blot & ELISA. The results showed thatantibody protein specificly paired with E. coli O157:H7. Initially determine the specificity of antibodies by recombinant methods silica nanoparticles attached to antibodies and staining with E. coli O157:H7 and examined under a microscope. Thereby creating a scientific basis for the study of microbial diagnostic kit created in food samples as well as providing additional sources of antibody therapy of poisoning caused by bacteria E. coli O157:H7.
Lung cancer is one of the most severe and frequent types of cancer in men, from 50 to 70 years of... more Lung cancer is one of the most severe and frequent types of cancer in men, from 50 to 70 years of age. There are two main types: small cell lung cancer and non-small cell lung cancer. Non-small cell lung cancer (NSCLC) accounts for about 80%. CYFRA21-1 antigen is associated explicitly with NSCLC according to recent studies on lung cancer and the mechanism of lung cancer generation. In the serum of healthy people, CYFRA21-1 antigen content makes up = 3.3 ng/mL. However, for those who appear NSCLC, this content increases very quickly. The concentration of CYFRA21-1 antigen in serum is directly proportional to the stage of cancer development. The higher the concentration, the shorter the lifetime of the patient. Therefore, it is possible to study the increase in serum CYFRA21-1 levels as a sign of early identification of NSCLC. The Phage Display Real-time Immuno-PCR method (PDRTI-PCR) was used in this study to quantify CYFRA21-1 antigen in NSCLC patients. PDRTI-PCR method has a combination of specificity of ELISA with sensitivity of Real-time PCR technique and phage display technique. Quantification of CYFRA21-1 antigen by PDRTI-PCR method provides reliable sensitivity and accuracy, which is the basis for creating an NSCLC early diagnosis kit.
The gene encoding the recombinant peptide has been synthesized by chemical method and it was suce... more The gene encoding the recombinant peptide has been synthesized by chemical method and it was sucessfully expressed in E. coli . The new method was developed for detection of the antimicrobial activities of the pure recombinant peptide which after has been purified on affinity chromatography and cleaved by the factor Xa.
Antibodies are immunoglobulin molecules which originally are glycoprotein that play role to ident... more Antibodies are immunoglobulin molecules which originally are glycoprotein that play role to identify and neutralize foreign agents such as viruses or bacteria. In this study, antibody protein recombine specificity of E. coli O157:H7 which was purified using hybrid methods. Next, antibody activity was tested by the methods as Dot Blot, Western Blot & ELISA. The results showed thatantibody protein specificly paired with E. coli O157:H7. Initially determine the specificity of antibodies by recombinant methods silica nanoparticles attached to antibodies and staining with E. coli O157:H7 and examined under a microscope. Thereby creating a scientific basis for the study of microbial diagnostic kit created in food samples as well as providing additional sources of antibody therapy of poisoning caused by bacteria E. coli O157:H7.
Lung cancer is one of the most severe and frequent types of cancer in men, from 50 to 70 years of... more Lung cancer is one of the most severe and frequent types of cancer in men, from 50 to 70 years of age. There are two main types: small cell lung cancer and non-small cell lung cancer. Non-small cell lung cancer (NSCLC) accounts for about 80%. CYFRA21-1 antigen is associated explicitly with NSCLC according to recent studies on lung cancer and the mechanism of lung cancer generation. In the serum of healthy people, CYFRA21-1 antigen content makes up = 3.3 ng/mL. However, for those who appear NSCLC, this content increases very quickly. The concentration of CYFRA21-1 antigen in serum is directly proportional to the stage of cancer development. The higher the concentration, the shorter the lifetime of the patient. Therefore, it is possible to study the increase in serum CYFRA21-1 levels as a sign of early identification of NSCLC. The Phage Display Real-time Immuno-PCR method (PDRTI-PCR) was used in this study to quantify CYFRA21-1 antigen in NSCLC patients. PDRTI-PCR method has a combination of specificity of ELISA with sensitivity of Real-time PCR technique and phage display technique. Quantification of CYFRA21-1 antigen by PDRTI-PCR method provides reliable sensitivity and accuracy, which is the basis for creating an NSCLC early diagnosis kit.
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