The computer skills present an economic role for all countries both individually and politically ... more The computer skills present an economic role for all countries both individually and politically in the current globalized world. The development of these skills requests the modernization of scholar and academic educational spaces. This modernization is necessary as it may allow better opportunities for the new generations as well as instruments to face the current social and economic challenges. However, the technology alone does not guarantee improvements in education and will depend on the pedagogical strategies of teaching and learning including the use of it as supporting and teaching tools. On that purpose, the offering of new alternatives for the elaboration and development of new methodological strategies for teaching biotechnology, a strategic worldwide area, is very important. The analysis of online tools in science education showed that they are very concentrated in the areas of basic technological science (eg. physics, chemistry and biology). Despite the closeness, ther...
Regulatory toxicology and pharmacology : RTP, 2017
The application of in silico methods is increasing on toxicological risk prediction for human and... more The application of in silico methods is increasing on toxicological risk prediction for human and environmental health. This work aimed to evaluate the performance of three in silico freeware models (OSIRIS v.2.0, LAZAR, and Toxtree) on the prediction of carcinogenicity and mutagenicity of thirty-eight volatile organic compounds (VOC) related to chemical risk assessment for occupational exposure. Theoretical data were compared with assessments available in international databases. Confusion matrices and ROC curves were used to evaluate the sensitivity, specificity, and accuracy of each model. All three models (OSIRIS, LAZAR and Toxtree) were able to identify VOC with a potential carcinogenicity or mutagenicity risk for humans, however presenting differences concerning the specificity, sensitivity, and accuracy. The best predictive performances were found for OSIRIS and LAZAR for carcinogenicity and OSIRIS for mutagenicity, as these softwares presented a combination of negative predi...
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
The computer skills present an economic role for all countries both individually and politically ... more The computer skills present an economic role for all countries both individually and politically in the current globalized world. The development of these skills requests the modernization of scholar and academic educational spaces. This modernization is necessary as it may allow better opportunities for the new generations as well as instruments to face the current social and economic challenges. However, the technology alone does not guarantee improvements in education and will depend on the pedagogical strategies of teaching and learning including the use of it as supporting and teaching tools. On that purpose, the offering of new alternatives for the elaboration and development of new methodological strategies for teaching biotechnology, a strategic worldwide area, is very important. The analysis of online tools in science education showed that they are very concentrated in the areas of basic technological science (eg. physics, chemistry and biology). Despite the closeness, ther...
Regulatory toxicology and pharmacology : RTP, 2017
The application of in silico methods is increasing on toxicological risk prediction for human and... more The application of in silico methods is increasing on toxicological risk prediction for human and environmental health. This work aimed to evaluate the performance of three in silico freeware models (OSIRIS v.2.0, LAZAR, and Toxtree) on the prediction of carcinogenicity and mutagenicity of thirty-eight volatile organic compounds (VOC) related to chemical risk assessment for occupational exposure. Theoretical data were compared with assessments available in international databases. Confusion matrices and ROC curves were used to evaluate the sensitivity, specificity, and accuracy of each model. All three models (OSIRIS, LAZAR and Toxtree) were able to identify VOC with a potential carcinogenicity or mutagenicity risk for humans, however presenting differences concerning the specificity, sensitivity, and accuracy. The best predictive performances were found for OSIRIS and LAZAR for carcinogenicity and OSIRIS for mutagenicity, as these softwares presented a combination of negative predi...
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extend... more The aim of this study was to characterize the KPC-type carbapenem-hydrolysing b-lactamase, extended-spectrum b-lactamases (ESBLs) and class 1 integrons among nosocomial Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. Methods: MICs were determined and isolates were screened for ESBLs, metallo-b-lactamases (MBLs) and class A carbapenemase-producing phenotypes. The main b-lactamases resistance genes (bla TEM , bla SHV , bla CTX-M , bla KPC , bla IMP and bla VIM) and class 1 integrons were detected by PCR followed by DNA sequencing. The genetic relatedness of isolates was determined by PFGE. Results: All K. pneumoniae isolates were positive for ESBL and class A carbapenemase production and negative for MBL production. All isolates were resistant to all b-lactam antibiotics, ciprofloxacin and gentamicin, being susceptible only to tigecycline and polymyxin B. The bla KPC-2 , bla CTX-M-1 , bla CTX-M-2 , bla CTX-M-8 and bla SHV-11 genes were detected. PFGE analysis revealed two clonal types among KPC-producing isolates, both identified in the same hospital. Conclusions: Our findings should alert medical authorities to implement stringent methods for the detection and spread control of emerging KPC-2 carbapenemases in the hospital setting in Brazil.
Uploads
Papers by Lília Guerra