Papers by Koyam Morales PhD
Frontiers in Molecular Neuroscience, Oct 28, 2022
and Bonansco C (2022) Wnt-5a induces the conversion of silent to functional synapses in the hippo... more and Bonansco C (2022) Wnt-5a induces the conversion of silent to functional synapses in the hippocampus.
Frontiers in Molecular Neuroscience
Synapse unsilencing is an essential mechanism for experience-dependent plasticity. Here, we showe... more Synapse unsilencing is an essential mechanism for experience-dependent plasticity. Here, we showed that the application of the ligand Wnt-5a converts glutamatergic silent synapses into functional ones by increasing both α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and N-methyl-D-aspartate (NMDA) currents (IAMPA and INMDA, respectively). These effects were mimicked by the hexapeptide Foxy-5 and inhibited by secreted frizzled-related protein sFRP-2. INMDA potentiation was produced by increased synaptic potency, followed by an increase in the probability of release (Pr), even in the presence of 7-nitro-2,3-dioxo-1,4-dihydroquinoxaline-6-carbonitrile (CNQX). At a longer time of Wnt-5a exposure, the Pr increments were higher in INMDA than in IAMPA. In the presence of NMDAR inhibitors, Wnt-5a-induced conversion was fully inhibited in 69.0% of silent synapses, whereas in the remaining synapses were converted into functional one. Our study findings showed that the Wnt-5a-act...
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Papers by Koyam Morales PhD