T HERE is an increasing worldwide demand for seafood due to the awareness of fish as an important... more T HERE is an increasing worldwide demand for seafood due to the awareness of fish as an important protein source for a growing population. However, the diseases caused by multi-drug resistant (MDR) bacterial infections and the low survival rate of the fish represent significant challenges to fish farmers. The virulence and pathogenicity of bacteria are often enhanced when growing as a biofilm. Therefore, a variety of new antimicrobial drugs has attracted wide attention in treating fish pathogen bacteria. Herein, the efficiency of macroalgal extracts as an antibacterial agent against MDR Staphylococcus aureus bacteria isolated from Nile tilapia (Oreochromis niloticus) was studied. Out of 200 bacterial isolates, 50 strains were identified as S. aureus. Of these strains, 37 were MDR and showed a potential role in the production of virulence factors, including staphylokinase (70.2%), lecithinase (81%), protease (56.7%) and lipase (59.4%). Significant production of biofilm virulence factor by MDR S. aureus strains was also observed from the quantitative and qualitative analysis. Four algal species namely Jania rubens, Ulva lactuca, Sargassum vulgare, and Sargassum fusiforme were tested for their antibacterial activity against MDR S. aureus strains. Of those, S. vulgare diethyl ether extract showed the highest antibacterial. In addition, GC-MS analysis revealed 20 identified components in S. vulgare diethyl ether extract, in which Longifolene was dominant (16.5%). This study thus established the possibility of developing an antibacterial agent to combat developing MDR S. aureus and biofilm-related infections in Nile tilapia.
Egyptian Journal of Genetics and Cytology, Jul 1, 2016
alinity stress is the most serious environmental challenge in cereal productivity in many arid an... more alinity stress is the most serious environmental challenge in cereal productivity in many arid and semi-arid regions (Clark and Duncan, 1993). It has reached 40% of the irrigated land and 2.1% of the globe and 25% of the irrigated land is affected by salt stress. The increase of salt-affected soils is referred to poor soil and water management in the irrigated areas, and become a great importance for agriculture production in this region (Sayar et al., 2010). MATERIALS AND METHODS Plant materials: Fifteen Egyptian barley cultivars were used, namely
Journal of Agricultural Chemistry and Biotechnology (Print), May 1, 2019
Out of sixteen Streptomyces sp. isolates were isolated from root rhizosphere soil of tomato plant... more Out of sixteen Streptomyces sp. isolates were isolated from root rhizosphere soil of tomato plants. Four isolates coded; C1, 8SW, 30SW and 11SA were selected as the highest antibiotic productivity. The highest records of the antibiotic productivity were 21 and 25 mm as inhibition zones obtained from the original strains 8SW and 11SA after 10 days using E. coli DH-5α as a tester. The highest records of the antibiotic productivity were 12 mm and 11 mm when used Aspergillus niger and Saccharomyces cerevisiae as testers. Three of the four tested Streptomyces isolates i.e. C1, 8SW and 11SA exhibited highly inhibited (31 mm) of the two fungal plant pathogens. Using RAPD and SRAP marker the clustering of tested isolates were different from each other, RAPD marker could be grouped isolates (C1 and 8SW), (30SW and 11SA) to two clusters. However, SRAP grouped isolates (C1, 8SW and 30SW) in one cluster, however isolate 11SA clustered separately. Based on DNA sequences using 16S rDNA the data showed that isolates C1 and 8SW belonged to Streptomyces albidoflavus and isolates 30SW and 11SA were belonged to Streptomyces bobbili. For more accuracy the isolate C1 aligned with all Streptomyces in gene bank and confirmed in belonged to S. albidoflavus.
Egyptian Journal of Genetics and Cytology, Jul 1, 2016
ilapia is the most species for fresh water aquaculture and its original habitat is Africa and the... more ilapia is the most species for fresh water aquaculture and its original habitat is Africa and the southwestern Middle East, even spread in worldwide. It is known as one of the main sources of animal protein in the future. Usually live in fresh water, also can live in different salt content of the water. It has a strong adaptability, in the waters of an area of narrow also can breed Badawy (1998), even in the rice fields to grow and have strong adaptability to less dissolved oxygen in the water. The vast majority of tilapia is omnivorous, eating water plants and debris (
Defoliating insects can cause significant damage to soybean plants in Egypt. The cotton leaf worm... more Defoliating insects can cause significant damage to soybean plants in Egypt. The cotton leaf worm (Spodoptera littoralis) is a major pest of soybean and important economic insect pests in Egypt than other insect pests. Susceptibility of 15 soybean genotypes to infestation of cotton leaf worm Spodoptera littoralis were studied under natural field conditions. The relationship between agro-morphological and molecular DNA in relation to leaves defoliation were also studied. Defoliation of tested plants by the cotton leaf worm, in the field screenings system showed the genotypes L105 and L153 recorded the highest values of insect resistant and recorded defoliation ranking from zero to 0.1 respectively, followed by genotypes named H10L10A, L162, and H1L1. Whereas, AGS-129 genotype showed a maximum insect susceptible had defoliation score (4.0) indicating heavy insect feeding, over both seasons. Moreover, highest values of seed yield/fed recorded by L 153, H10L10A and H1L1, this attributed...
Soil salinity is one of the abiotic stresses that cause a significant reduction in barley product... more Soil salinity is one of the abiotic stresses that cause a significant reduction in barley production. Understanding the phenotypic and genetic diversity among Barley genotypes is necessary to improve barley salt tolerance. Herne comprehensive sets of morph-physiological, grain quality traits and Simple Sequence Repeat (SSR) markers combined with economic analysis were done to determine the phenotypic and genetic variation of eight barley genotypes under salinity stress during seasons 2019/2020 and 2020/2021. High genetic variation was observed among studied genotypes for all measured traits. Salinization caused a significant increase in (Sodium content, soluble carbohydrate content, and crude protein content %) in sensitive genotypes (Giza 132 and line 1). SSRs markers generated clear patterns with high polymorphism with 31 alleles by an average of 2.07 alleles per locus. Out of 15 SSR markers, nine
Journal of the Faculty of Agriculture, Kyushu University
Genetic diversity and relationships among 19 Egyptian barley (Hordeum vulgare) genotypes includin... more Genetic diversity and relationships among 19 Egyptian barley (Hordeum vulgare) genotypes including cultivars and promising lines were evaluated. The 19 genotypes were used to detect marker associated with resistance to leaf rust using simple sequence repeat (SSR). Greenhouse was screening to evaluated the seedling stage of leaf rust reaction for the studied genotypes and the field screening analysis was carried out in the Experimental Research Station of Sakha during two growing seasons 2019/2020 and 2020/2021 to evaluate some morphological traits and adult leaf rust reaction among 19 barley genotypes. The genotypes were highly diverse in leaf rust reaction; Giza 131,133,132,136,137, line 6 and line 7 were resistant. Principal components analysis PCA was performed explaining about 53.34 % of total variance which was highly informative. SSR marker, Bmag692, discriminate the resistance and susceptible barley genotypes which it could be useful for the genetic diversity studies of leaf rust in barley. These results will be useful for barley management in terms of biodiversity protection and design of new crosses for disease resistance to leaf rust breeding program.
Net blotch infection is the most widespread and harmful disease for barley that caused substantia... more Net blotch infection is the most widespread and harmful disease for barley that caused substantial losses grain yield and quality, in this study Greenhouse and field experiments were carried out to recorded the net blotch reaction at seedling and adult stage of twenty barley genotypes during two growing seasons 2020/2021 and 2021/2022 .Start Codon Targeted (SCoT) markers were used to study the genetic variances and relationships among barley genotypes against net blotch reaction. The expression of severity to net blotch infection based on agro- physiological traits and net blotch reaction studied showed that Line 3 and Giza 2000 genotypes appeared the highest net blotch infection response that had lowest phenol content, chlorophyll fluorescence (FV/FM ratio) and total chlorophyll content SPAD were (42.52 mg/100g 0.510, 39. 53 SPAD reading and 43.76 mg/100g, 0.543 and 40.50 SPAD reading) respectively. However Giza 132, Giza 134, Giza 137, Giza 136, Line 4, line 5 and line 7 were prov...
International Journal of Plant and Soil Science, Dec 15, 2022
Nowadays, water scarcity is a great danger to agriculture development, in semiarid and arid regio... more Nowadays, water scarcity is a great danger to agriculture development, in semiarid and arid regions beside climate change risks. Egypt is suffering from scarce water resources for agriculture; it is of high priority to rationalize irrigation water use. During two seasons, 2019/2020 and 2020/2021 the experimental were carried out to investigate the effect of three different irrigation water treatments (I 1 at 45, I 2 at 60 and I 3 at 75% depletion of available soil moisture) on some photosynthetic, agronomical, grain quality parameters, water productivity for eight barley genotypes. Also, classify them on the SSR molecular level. The results showed that there were high genetic variations found among the eight genotypes with significant responses to irrigation water treatments. Rationalize irrigation water from I 1 to I 3 had a negative effect on all studied phenotypic traits, whereas had an appositive effect on leaf diffusive resistance and cured protein content and inducement all genotypes to flower early by average increasing (27.7, 12.7 and 7.08%) respectively. Giza 138,
Chilean Journal of Agricultural Research, Jun 1, 2022
Salinity is a main obstacle of rice (Oryza sativa L.) cultivation. Selecting rice genotypes for s... more Salinity is a main obstacle of rice (Oryza sativa L.) cultivation. Selecting rice genotypes for salinity tolerance based on phenotypic characteristics alone is inefficient and less reliable, finally will delay progress in rice breeding program. The recent advantages of molecular markers such as simple sequence repeat (SSR) could be used to detect associated salt tolerance markers in rice. This study was conducted to detect genetic variation within some rice genotypes using SSR markers and to identify salt tolerance in the studied genotypes. Forty-five rice genotypes were evaluated for their agromorphological characteristics under non-stress and saline conditions for two growing seasons in 2018 and 2019. Using 21 SSR primers located on chromosome 8, only 18 SSR primer generated polymorphic patterns with a total of 76 alleles, whereas the other 3 primers were monomorphic. The number of alleles per locus ranged from 2 to 6 alleles with an average of 4.22 alleles per locus. The polymorphic information content (PIC) values varied from 0.30 (RM342) to 0.71 (RM6976) with an average PIC of 0.49. Out of the 18 polymorphic markers only 5 primers (RM 6976, RM7631, RM 5556, RM152 and RM342) perfectly distinguished rice genotypes. The best preforming genotypes under salinity stress were N22,
In view of the great strategic importance of wheat as a global food crop besides, also the great ... more In view of the great strategic importance of wheat as a global food crop besides, also the great damage it inflicts on it due to salt-stress, this study was launched as an attempt to understand the nature of this crisis and come up with clear scientific recommendations in this regard. The most important aspect of this investigation was evaluating of a set of wheat genotypes with different response to salt stress tolerance during conducting the selection process on some traits such as yield and its components besides, some physiological attributes and related to salinity tolerance such as osmotic adjustment, proline and glycine betaine contents under normal and salinity conditions. The wheat accessions divided into two group where, the first one included parents namely; Sakha 8, Shandweel 1, Masr 1, Giza 171, Sakha 94, Gimeaza 11 and Gimeaza 12, respectively. While, the second group was 21 F1 wheat crosses obtained from half diallel crossing among the seven wheat genotypes mentioned ...
Field experiments carried out at the Experimental Research Station of Nubaria, during two seasons... more Field experiments carried out at the Experimental Research Station of Nubaria, during two seasons of 2016/2017 and 2017/2018 to characterize the Mediterranean barley (Egyptian, Tunisian, Algerian and Morocco) varieties grown under water stress (40% WHC) and normal irrigation (75% WHC). The barley grain yield had a positive and highly significant correlation with drought stress indices (STI, MP and GMP), indicating that STI, MP and GMP more suitable indices for selecting barley varieties to drought stress tolerance. Results also concluded that the Egyptian varieties (Giza 131, Giza 127, Giza 2000, Giza 130), Tunisian varieties (Kebili 3,Tozeur 2, Sidi-Bou), Algerian varieties (Nailia and Techedrett and Temacine) and Morocco varieties (Laanaceur, Massine and Amira), gained the highest values of most drought tolerance indices and produced greater grain yield proving their drought stress tolerance as compare to Giza 125 and Giza 126 (Egyptian), Tombari, Raihane (Tunisian), Ksar-Megrine, Ras El-Mouch (Algerian) and Tamellalet, Adrar (Morocco) which was the lowest grain yield under water stress condition. The data obtained from this study could be useful for barley breeders, agronomists and grain producers in order to increase grain yield under water stress condition of arid and semi-arid regions in North African countries.
Journal of Genetic Engineering and Biotechnology, 2018
In order to investigate the effects of drought stress on germination components of barley cultiva... more In order to investigate the effects of drought stress on germination components of barley cultivars, a laboratory experiment was conducted in a factorial randomized complete design with four replications. The controlled experiment included ten of Egyptian barley cultivars namely; (Giza 123, 124, 125, 126, 127, 129, 130, 134, 135 and 2000) as first factor. The second factor included 4 levels of drought stress inducer by applying 0, 5, 10 and 20% of polyethylene glycol-6000 (PEG) which is equivalent to four osmotic potential levels including À0.001, À0.27, À0.54 and À1.09 MPa, respectively. The results showed that, the highest reduction was related to the drought level of 20% PEG among the barley cultivars. The best cultivars in terms of germination traits were Giza 134, Giza 127, and Giza 126 this indicate their tolerance to drought stress and Giza 130, 135, 2000 cultivars was moderately tolerance and remaining is less tolerance. The protein band 27 kDa and 78 kDa showed high intensity after stress in almost all cultivars. Those two protein bands their exciting was very clear in treated barley leaf tissue. It could be related to dehydrine and oxygen evolving enhancer protein 2 (OEE2) which involved in drought stress tolerance response. Cultivars Giza 127, 130 and 134 showed highest tolerance response under drought stress. The antioxidant enzymes PAGE pattern of Peroxidase (POX), Sodium dismutase (SOD) and Ascorbate peroxidase (APX) for Barley cultivars under drought stress revealed a high activities for Giza 126, 127, 134, 136 and 2000 under À0.5 MPa osmotic stress by PEG in most of their isoforms. Based on similarity coefficient values the highest values were 1.0 with 100% similarly between tolerant cultivars Giza 130 and Giza 127. Similarly between the susceptible cultivars 125 and Giza 129 was 60%.These data confirmed by the growth parameters which we ranked as tolerant to drought stress.
Journal of Umm Al-Qura University for Applied Sciences
White-spotted stink bugs, were noticed as a first time in a few numbers of the Egyptian barley cu... more White-spotted stink bugs, were noticed as a first time in a few numbers of the Egyptian barley cultivar Giza 138 plants in mid of January of 2019/2020 season at soil improvement and conservation Research department at Sakha Agricultural Research station, farm, Kafr El-Sheikh Government, Egypt. Morphological identification and DNA barcoding of mitochondrial gene cytochrome c oxidase subunit I (COI) were used identified the species of collected bugs. The results revealed a new record of Eysarcoris ventralis (Westwood 1837) (Hemiptera: Heteroptera: Pentatomidae). The results of pest insect survey of directly accounts on barley cultivar weekly during two growing winter 2019/2020 and 2019/2021 seasons displayed a significant effect of seasons on the E. ventralis population density. Positive significantly correlation was found between the E. ventralis population density and growth stages of barley under 2019/2020 and 2019/2021 seasons by (0.311 and 0.531) respectively. Regression coeffici...
The scarcity of water resources in arid and semi-arid areas is considered a threat to agricultura... more The scarcity of water resources in arid and semi-arid areas is considered a threat to agricultural sustainability. Therefore, approaches are needed to rationalize use of irrigation water without reducing crop productivity or degrading soil properties. The objective of this study was to investigate the effect of different organic amendments (O1 = control, O2 = compost, and O3 = vermicompost) combined with different rates of nano- zinc foliar spraying (Zn1 = 0, Zn2 = 1 and Zn3 = 2 gm/L), under irrigation supplements (I1 = 100%, I2 = 85%, and I3 = 65% of water requirements) on clay soil characteristics, on the production of Egyptian barley Giza 126. Over two successive winter growing seasons, 2018/2019 and 2019/2020, field experiments were conducted as a split-split plot design with three replications. The results show that using vermicompost is an appropriate organic amendment to improve the physical and chemical properties of soils as compared with compost. Application of vermicompos...
otato (Solanum tuberosum L.) is an economically important vegetable crop in Egypt. It is the four... more otato (Solanum tuberosum L.) is an economically important vegetable crop in Egypt. It is the fourth most important crop by volume of production; it is
T HERE is an increasing worldwide demand for seafood due to the awareness of fish as an important... more T HERE is an increasing worldwide demand for seafood due to the awareness of fish as an important protein source for a growing population. However, the diseases caused by multi-drug resistant (MDR) bacterial infections and the low survival rate of the fish represent significant challenges to fish farmers. The virulence and pathogenicity of bacteria are often enhanced when growing as a biofilm. Therefore, a variety of new antimicrobial drugs has attracted wide attention in treating fish pathogen bacteria. Herein, the efficiency of macroalgal extracts as an antibacterial agent against MDR Staphylococcus aureus bacteria isolated from Nile tilapia (Oreochromis niloticus) was studied. Out of 200 bacterial isolates, 50 strains were identified as S. aureus. Of these strains, 37 were MDR and showed a potential role in the production of virulence factors, including staphylokinase (70.2%), lecithinase (81%), protease (56.7%) and lipase (59.4%). Significant production of biofilm virulence factor by MDR S. aureus strains was also observed from the quantitative and qualitative analysis. Four algal species namely Jania rubens, Ulva lactuca, Sargassum vulgare, and Sargassum fusiforme were tested for their antibacterial activity against MDR S. aureus strains. Of those, S. vulgare diethyl ether extract showed the highest antibacterial. In addition, GC-MS analysis revealed 20 identified components in S. vulgare diethyl ether extract, in which Longifolene was dominant (16.5%). This study thus established the possibility of developing an antibacterial agent to combat developing MDR S. aureus and biofilm-related infections in Nile tilapia.
Egyptian Journal of Genetics and Cytology, Jul 1, 2016
alinity stress is the most serious environmental challenge in cereal productivity in many arid an... more alinity stress is the most serious environmental challenge in cereal productivity in many arid and semi-arid regions (Clark and Duncan, 1993). It has reached 40% of the irrigated land and 2.1% of the globe and 25% of the irrigated land is affected by salt stress. The increase of salt-affected soils is referred to poor soil and water management in the irrigated areas, and become a great importance for agriculture production in this region (Sayar et al., 2010). MATERIALS AND METHODS Plant materials: Fifteen Egyptian barley cultivars were used, namely
Journal of Agricultural Chemistry and Biotechnology (Print), May 1, 2019
Out of sixteen Streptomyces sp. isolates were isolated from root rhizosphere soil of tomato plant... more Out of sixteen Streptomyces sp. isolates were isolated from root rhizosphere soil of tomato plants. Four isolates coded; C1, 8SW, 30SW and 11SA were selected as the highest antibiotic productivity. The highest records of the antibiotic productivity were 21 and 25 mm as inhibition zones obtained from the original strains 8SW and 11SA after 10 days using E. coli DH-5α as a tester. The highest records of the antibiotic productivity were 12 mm and 11 mm when used Aspergillus niger and Saccharomyces cerevisiae as testers. Three of the four tested Streptomyces isolates i.e. C1, 8SW and 11SA exhibited highly inhibited (31 mm) of the two fungal plant pathogens. Using RAPD and SRAP marker the clustering of tested isolates were different from each other, RAPD marker could be grouped isolates (C1 and 8SW), (30SW and 11SA) to two clusters. However, SRAP grouped isolates (C1, 8SW and 30SW) in one cluster, however isolate 11SA clustered separately. Based on DNA sequences using 16S rDNA the data showed that isolates C1 and 8SW belonged to Streptomyces albidoflavus and isolates 30SW and 11SA were belonged to Streptomyces bobbili. For more accuracy the isolate C1 aligned with all Streptomyces in gene bank and confirmed in belonged to S. albidoflavus.
Egyptian Journal of Genetics and Cytology, Jul 1, 2016
ilapia is the most species for fresh water aquaculture and its original habitat is Africa and the... more ilapia is the most species for fresh water aquaculture and its original habitat is Africa and the southwestern Middle East, even spread in worldwide. It is known as one of the main sources of animal protein in the future. Usually live in fresh water, also can live in different salt content of the water. It has a strong adaptability, in the waters of an area of narrow also can breed Badawy (1998), even in the rice fields to grow and have strong adaptability to less dissolved oxygen in the water. The vast majority of tilapia is omnivorous, eating water plants and debris (
Defoliating insects can cause significant damage to soybean plants in Egypt. The cotton leaf worm... more Defoliating insects can cause significant damage to soybean plants in Egypt. The cotton leaf worm (Spodoptera littoralis) is a major pest of soybean and important economic insect pests in Egypt than other insect pests. Susceptibility of 15 soybean genotypes to infestation of cotton leaf worm Spodoptera littoralis were studied under natural field conditions. The relationship between agro-morphological and molecular DNA in relation to leaves defoliation were also studied. Defoliation of tested plants by the cotton leaf worm, in the field screenings system showed the genotypes L105 and L153 recorded the highest values of insect resistant and recorded defoliation ranking from zero to 0.1 respectively, followed by genotypes named H10L10A, L162, and H1L1. Whereas, AGS-129 genotype showed a maximum insect susceptible had defoliation score (4.0) indicating heavy insect feeding, over both seasons. Moreover, highest values of seed yield/fed recorded by L 153, H10L10A and H1L1, this attributed...
Soil salinity is one of the abiotic stresses that cause a significant reduction in barley product... more Soil salinity is one of the abiotic stresses that cause a significant reduction in barley production. Understanding the phenotypic and genetic diversity among Barley genotypes is necessary to improve barley salt tolerance. Herne comprehensive sets of morph-physiological, grain quality traits and Simple Sequence Repeat (SSR) markers combined with economic analysis were done to determine the phenotypic and genetic variation of eight barley genotypes under salinity stress during seasons 2019/2020 and 2020/2021. High genetic variation was observed among studied genotypes for all measured traits. Salinization caused a significant increase in (Sodium content, soluble carbohydrate content, and crude protein content %) in sensitive genotypes (Giza 132 and line 1). SSRs markers generated clear patterns with high polymorphism with 31 alleles by an average of 2.07 alleles per locus. Out of 15 SSR markers, nine
Journal of the Faculty of Agriculture, Kyushu University
Genetic diversity and relationships among 19 Egyptian barley (Hordeum vulgare) genotypes includin... more Genetic diversity and relationships among 19 Egyptian barley (Hordeum vulgare) genotypes including cultivars and promising lines were evaluated. The 19 genotypes were used to detect marker associated with resistance to leaf rust using simple sequence repeat (SSR). Greenhouse was screening to evaluated the seedling stage of leaf rust reaction for the studied genotypes and the field screening analysis was carried out in the Experimental Research Station of Sakha during two growing seasons 2019/2020 and 2020/2021 to evaluate some morphological traits and adult leaf rust reaction among 19 barley genotypes. The genotypes were highly diverse in leaf rust reaction; Giza 131,133,132,136,137, line 6 and line 7 were resistant. Principal components analysis PCA was performed explaining about 53.34 % of total variance which was highly informative. SSR marker, Bmag692, discriminate the resistance and susceptible barley genotypes which it could be useful for the genetic diversity studies of leaf rust in barley. These results will be useful for barley management in terms of biodiversity protection and design of new crosses for disease resistance to leaf rust breeding program.
Net blotch infection is the most widespread and harmful disease for barley that caused substantia... more Net blotch infection is the most widespread and harmful disease for barley that caused substantial losses grain yield and quality, in this study Greenhouse and field experiments were carried out to recorded the net blotch reaction at seedling and adult stage of twenty barley genotypes during two growing seasons 2020/2021 and 2021/2022 .Start Codon Targeted (SCoT) markers were used to study the genetic variances and relationships among barley genotypes against net blotch reaction. The expression of severity to net blotch infection based on agro- physiological traits and net blotch reaction studied showed that Line 3 and Giza 2000 genotypes appeared the highest net blotch infection response that had lowest phenol content, chlorophyll fluorescence (FV/FM ratio) and total chlorophyll content SPAD were (42.52 mg/100g 0.510, 39. 53 SPAD reading and 43.76 mg/100g, 0.543 and 40.50 SPAD reading) respectively. However Giza 132, Giza 134, Giza 137, Giza 136, Line 4, line 5 and line 7 were prov...
International Journal of Plant and Soil Science, Dec 15, 2022
Nowadays, water scarcity is a great danger to agriculture development, in semiarid and arid regio... more Nowadays, water scarcity is a great danger to agriculture development, in semiarid and arid regions beside climate change risks. Egypt is suffering from scarce water resources for agriculture; it is of high priority to rationalize irrigation water use. During two seasons, 2019/2020 and 2020/2021 the experimental were carried out to investigate the effect of three different irrigation water treatments (I 1 at 45, I 2 at 60 and I 3 at 75% depletion of available soil moisture) on some photosynthetic, agronomical, grain quality parameters, water productivity for eight barley genotypes. Also, classify them on the SSR molecular level. The results showed that there were high genetic variations found among the eight genotypes with significant responses to irrigation water treatments. Rationalize irrigation water from I 1 to I 3 had a negative effect on all studied phenotypic traits, whereas had an appositive effect on leaf diffusive resistance and cured protein content and inducement all genotypes to flower early by average increasing (27.7, 12.7 and 7.08%) respectively. Giza 138,
Chilean Journal of Agricultural Research, Jun 1, 2022
Salinity is a main obstacle of rice (Oryza sativa L.) cultivation. Selecting rice genotypes for s... more Salinity is a main obstacle of rice (Oryza sativa L.) cultivation. Selecting rice genotypes for salinity tolerance based on phenotypic characteristics alone is inefficient and less reliable, finally will delay progress in rice breeding program. The recent advantages of molecular markers such as simple sequence repeat (SSR) could be used to detect associated salt tolerance markers in rice. This study was conducted to detect genetic variation within some rice genotypes using SSR markers and to identify salt tolerance in the studied genotypes. Forty-five rice genotypes were evaluated for their agromorphological characteristics under non-stress and saline conditions for two growing seasons in 2018 and 2019. Using 21 SSR primers located on chromosome 8, only 18 SSR primer generated polymorphic patterns with a total of 76 alleles, whereas the other 3 primers were monomorphic. The number of alleles per locus ranged from 2 to 6 alleles with an average of 4.22 alleles per locus. The polymorphic information content (PIC) values varied from 0.30 (RM342) to 0.71 (RM6976) with an average PIC of 0.49. Out of the 18 polymorphic markers only 5 primers (RM 6976, RM7631, RM 5556, RM152 and RM342) perfectly distinguished rice genotypes. The best preforming genotypes under salinity stress were N22,
In view of the great strategic importance of wheat as a global food crop besides, also the great ... more In view of the great strategic importance of wheat as a global food crop besides, also the great damage it inflicts on it due to salt-stress, this study was launched as an attempt to understand the nature of this crisis and come up with clear scientific recommendations in this regard. The most important aspect of this investigation was evaluating of a set of wheat genotypes with different response to salt stress tolerance during conducting the selection process on some traits such as yield and its components besides, some physiological attributes and related to salinity tolerance such as osmotic adjustment, proline and glycine betaine contents under normal and salinity conditions. The wheat accessions divided into two group where, the first one included parents namely; Sakha 8, Shandweel 1, Masr 1, Giza 171, Sakha 94, Gimeaza 11 and Gimeaza 12, respectively. While, the second group was 21 F1 wheat crosses obtained from half diallel crossing among the seven wheat genotypes mentioned ...
Field experiments carried out at the Experimental Research Station of Nubaria, during two seasons... more Field experiments carried out at the Experimental Research Station of Nubaria, during two seasons of 2016/2017 and 2017/2018 to characterize the Mediterranean barley (Egyptian, Tunisian, Algerian and Morocco) varieties grown under water stress (40% WHC) and normal irrigation (75% WHC). The barley grain yield had a positive and highly significant correlation with drought stress indices (STI, MP and GMP), indicating that STI, MP and GMP more suitable indices for selecting barley varieties to drought stress tolerance. Results also concluded that the Egyptian varieties (Giza 131, Giza 127, Giza 2000, Giza 130), Tunisian varieties (Kebili 3,Tozeur 2, Sidi-Bou), Algerian varieties (Nailia and Techedrett and Temacine) and Morocco varieties (Laanaceur, Massine and Amira), gained the highest values of most drought tolerance indices and produced greater grain yield proving their drought stress tolerance as compare to Giza 125 and Giza 126 (Egyptian), Tombari, Raihane (Tunisian), Ksar-Megrine, Ras El-Mouch (Algerian) and Tamellalet, Adrar (Morocco) which was the lowest grain yield under water stress condition. The data obtained from this study could be useful for barley breeders, agronomists and grain producers in order to increase grain yield under water stress condition of arid and semi-arid regions in North African countries.
Journal of Genetic Engineering and Biotechnology, 2018
In order to investigate the effects of drought stress on germination components of barley cultiva... more In order to investigate the effects of drought stress on germination components of barley cultivars, a laboratory experiment was conducted in a factorial randomized complete design with four replications. The controlled experiment included ten of Egyptian barley cultivars namely; (Giza 123, 124, 125, 126, 127, 129, 130, 134, 135 and 2000) as first factor. The second factor included 4 levels of drought stress inducer by applying 0, 5, 10 and 20% of polyethylene glycol-6000 (PEG) which is equivalent to four osmotic potential levels including À0.001, À0.27, À0.54 and À1.09 MPa, respectively. The results showed that, the highest reduction was related to the drought level of 20% PEG among the barley cultivars. The best cultivars in terms of germination traits were Giza 134, Giza 127, and Giza 126 this indicate their tolerance to drought stress and Giza 130, 135, 2000 cultivars was moderately tolerance and remaining is less tolerance. The protein band 27 kDa and 78 kDa showed high intensity after stress in almost all cultivars. Those two protein bands their exciting was very clear in treated barley leaf tissue. It could be related to dehydrine and oxygen evolving enhancer protein 2 (OEE2) which involved in drought stress tolerance response. Cultivars Giza 127, 130 and 134 showed highest tolerance response under drought stress. The antioxidant enzymes PAGE pattern of Peroxidase (POX), Sodium dismutase (SOD) and Ascorbate peroxidase (APX) for Barley cultivars under drought stress revealed a high activities for Giza 126, 127, 134, 136 and 2000 under À0.5 MPa osmotic stress by PEG in most of their isoforms. Based on similarity coefficient values the highest values were 1.0 with 100% similarly between tolerant cultivars Giza 130 and Giza 127. Similarly between the susceptible cultivars 125 and Giza 129 was 60%.These data confirmed by the growth parameters which we ranked as tolerant to drought stress.
Journal of Umm Al-Qura University for Applied Sciences
White-spotted stink bugs, were noticed as a first time in a few numbers of the Egyptian barley cu... more White-spotted stink bugs, were noticed as a first time in a few numbers of the Egyptian barley cultivar Giza 138 plants in mid of January of 2019/2020 season at soil improvement and conservation Research department at Sakha Agricultural Research station, farm, Kafr El-Sheikh Government, Egypt. Morphological identification and DNA barcoding of mitochondrial gene cytochrome c oxidase subunit I (COI) were used identified the species of collected bugs. The results revealed a new record of Eysarcoris ventralis (Westwood 1837) (Hemiptera: Heteroptera: Pentatomidae). The results of pest insect survey of directly accounts on barley cultivar weekly during two growing winter 2019/2020 and 2019/2021 seasons displayed a significant effect of seasons on the E. ventralis population density. Positive significantly correlation was found between the E. ventralis population density and growth stages of barley under 2019/2020 and 2019/2021 seasons by (0.311 and 0.531) respectively. Regression coeffici...
The scarcity of water resources in arid and semi-arid areas is considered a threat to agricultura... more The scarcity of water resources in arid and semi-arid areas is considered a threat to agricultural sustainability. Therefore, approaches are needed to rationalize use of irrigation water without reducing crop productivity or degrading soil properties. The objective of this study was to investigate the effect of different organic amendments (O1 = control, O2 = compost, and O3 = vermicompost) combined with different rates of nano- zinc foliar spraying (Zn1 = 0, Zn2 = 1 and Zn3 = 2 gm/L), under irrigation supplements (I1 = 100%, I2 = 85%, and I3 = 65% of water requirements) on clay soil characteristics, on the production of Egyptian barley Giza 126. Over two successive winter growing seasons, 2018/2019 and 2019/2020, field experiments were conducted as a split-split plot design with three replications. The results show that using vermicompost is an appropriate organic amendment to improve the physical and chemical properties of soils as compared with compost. Application of vermicompos...
otato (Solanum tuberosum L.) is an economically important vegetable crop in Egypt. It is the four... more otato (Solanum tuberosum L.) is an economically important vegetable crop in Egypt. It is the fourth most important crop by volume of production; it is
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