International journal of community medicine and public health/International journall of community medicine and public health, Mar 30, 2024
The SARS-CoV-2 emergence in December 2019 in Wuhan, China, caused a devastating impact on numerou... more The SARS-CoV-2 emergence in December 2019 in Wuhan, China, caused a devastating impact on numerous countries and resulted in millions of casualties. 1,2 To mitigate risk and safeguard human lives, a widespread vaccination campaign is vital for the national and global pandemic response, especially in heavily populated countries such as the Philippines. 1 One of the primary factors to ensure the success of the vaccination campaigns is the willingness of the public to ABSTRACT Background: COVID-19 vaccination is essential for public health, aiming to limit virus transmission and severity of illness. The motivation for individuals to get vaccinated can vary and is influenced by a combination of factors. This study aimed to identify the factors that motivated Filipino residents in Cavite, Laguna, Batangas, Rizal, Quezon Province, or the Calabarzon region to get vaccinated with the SARS-CoV-2 vaccine. Methods: A descriptive cross-sectional design was utilized in this study. Researcher-made survey questionnaires were distributed online through Google forms. Study participants were either fully or partially vaccinated with SARS-CoV-2 vaccines or have received their booster shots. Descriptive statistics were used to examine the extrinsic and intrinsic factors toward uptake of SARS-CoV-2 vaccine. The participants' overall knowledge and attitude were scored using Bloom's cutoff point. Results: A total of 398 participants were approached from June to July 2022. Participants were 55.3% female, and the majority were 29 years and below (77.1%). Family/friends, lifestyle, government, community, and peers/associates were the extrinsic factors that motivated them to get vaccinated. As for the intrinsic factors, 86.4% showed adequate knowledge and 63.6% had positive attitude towards the SARS-CoV-2 vaccine. Conclusions: To have a successful vaccination campaign, both extrinsic and intrinsic factors must be considered when exploring the motivation of local residents to get vaccinated for COVID-19. Family/friends were the primary extrinsic factor, followed by lifestyle, government, community, and peers/associates. Most participants showed adequate knowledge and a positive attitude toward the SARS-CoV-2 vaccines. Focusing on these factors may address vaccine hesitancy.
The vacuolar-type H +-ATPase (V-ATPase) is a multisubunit proton pump that is involved in both in... more The vacuolar-type H +-ATPase (V-ATPase) is a multisubunit proton pump that is involved in both intra-and extracellular acidification processes throughout the body. Multiple homologs and splice variants of V-ATPase subunits are thought to explain its varied spatial and temporal expression pattern in different cell types. Recently subunit nomenclature was standardized with a total of 22 subunit variants identified. However this standardization did not accommodate the existence of splice variants and is therefore incomplete. Thus, we propose here an extension of subunit nomenclature along with a literature and sequence database scan for additional V-ATPase subunits. An additional 17 variants were pulled from a literature search while 4 uncharacterized potential subunit variants were found in sequence databases. These findings have been integrated with the current V-ATPase knowledge base to create a new V-ATPase subunit catalogue. It is envisioned this catalogue will form a new platform on which future studies into tissue-and organelle-specific V-ATPase expression, localization and function can be based.
We present rna22, a method for identifying mi-croRNA binding sites and their corresponding hetero... more We present rna22, a method for identifying mi-croRNA binding sites and their corresponding heteroduplexes. Rna22 does not rely upon cross-species conservation, is resilient to noise, and, unlike previous methods, it first finds putative microRNA binding sites in the sequence of interest, then identifies the targeting microRNA. Computationally, we show that rna22 identifies most of the currently known heteroduplexes. Experimentally, with luciferase assays, we demonstrate average repressions of 30% or more for 168 of 226 tested targets. The analysis suggests that some microRNAs may have as many as a few thousand targets, and that between 74% and 92% of the gene transcripts in four model genomes are likely under microRNA control through their untranslated and amino acid coding regions. We also extended the method's key idea to a low-error microRNA-precursordiscovery scheme; our studies suggest that the number of microRNA precursors in mammalian genomes likely ranges in the tens of thousands.
Citation for published version (APA): Abdel-Mageed, W. M., Lehri, B., Jarmusch, S. A., Miranda, K... more Citation for published version (APA): Abdel-Mageed, W. M., Lehri, B., Jarmusch, S. A., Miranda, K., Al-Wahaibi, L. H., Stewart, H. A., Jamieson, A. J., Jaspars, M., & Karlyshev, A. V. (2020). Whole genome sequencing of four bacterial strains from South Shetland Trench revealing biosynthetic and environmental adaptation gene clusters. Marine Genomics, 54, [100782]. https://doi.org/10.1016/j.margen.2020.100782
International journal of community medicine and public health/International journall of community medicine and public health, Mar 30, 2024
The SARS-CoV-2 emergence in December 2019 in Wuhan, China, caused a devastating impact on numerou... more The SARS-CoV-2 emergence in December 2019 in Wuhan, China, caused a devastating impact on numerous countries and resulted in millions of casualties. 1,2 To mitigate risk and safeguard human lives, a widespread vaccination campaign is vital for the national and global pandemic response, especially in heavily populated countries such as the Philippines. 1 One of the primary factors to ensure the success of the vaccination campaigns is the willingness of the public to ABSTRACT Background: COVID-19 vaccination is essential for public health, aiming to limit virus transmission and severity of illness. The motivation for individuals to get vaccinated can vary and is influenced by a combination of factors. This study aimed to identify the factors that motivated Filipino residents in Cavite, Laguna, Batangas, Rizal, Quezon Province, or the Calabarzon region to get vaccinated with the SARS-CoV-2 vaccine. Methods: A descriptive cross-sectional design was utilized in this study. Researcher-made survey questionnaires were distributed online through Google forms. Study participants were either fully or partially vaccinated with SARS-CoV-2 vaccines or have received their booster shots. Descriptive statistics were used to examine the extrinsic and intrinsic factors toward uptake of SARS-CoV-2 vaccine. The participants' overall knowledge and attitude were scored using Bloom's cutoff point. Results: A total of 398 participants were approached from June to July 2022. Participants were 55.3% female, and the majority were 29 years and below (77.1%). Family/friends, lifestyle, government, community, and peers/associates were the extrinsic factors that motivated them to get vaccinated. As for the intrinsic factors, 86.4% showed adequate knowledge and 63.6% had positive attitude towards the SARS-CoV-2 vaccine. Conclusions: To have a successful vaccination campaign, both extrinsic and intrinsic factors must be considered when exploring the motivation of local residents to get vaccinated for COVID-19. Family/friends were the primary extrinsic factor, followed by lifestyle, government, community, and peers/associates. Most participants showed adequate knowledge and a positive attitude toward the SARS-CoV-2 vaccines. Focusing on these factors may address vaccine hesitancy.
The vacuolar-type H +-ATPase (V-ATPase) is a multisubunit proton pump that is involved in both in... more The vacuolar-type H +-ATPase (V-ATPase) is a multisubunit proton pump that is involved in both intra-and extracellular acidification processes throughout the body. Multiple homologs and splice variants of V-ATPase subunits are thought to explain its varied spatial and temporal expression pattern in different cell types. Recently subunit nomenclature was standardized with a total of 22 subunit variants identified. However this standardization did not accommodate the existence of splice variants and is therefore incomplete. Thus, we propose here an extension of subunit nomenclature along with a literature and sequence database scan for additional V-ATPase subunits. An additional 17 variants were pulled from a literature search while 4 uncharacterized potential subunit variants were found in sequence databases. These findings have been integrated with the current V-ATPase knowledge base to create a new V-ATPase subunit catalogue. It is envisioned this catalogue will form a new platform on which future studies into tissue-and organelle-specific V-ATPase expression, localization and function can be based.
We present rna22, a method for identifying mi-croRNA binding sites and their corresponding hetero... more We present rna22, a method for identifying mi-croRNA binding sites and their corresponding heteroduplexes. Rna22 does not rely upon cross-species conservation, is resilient to noise, and, unlike previous methods, it first finds putative microRNA binding sites in the sequence of interest, then identifies the targeting microRNA. Computationally, we show that rna22 identifies most of the currently known heteroduplexes. Experimentally, with luciferase assays, we demonstrate average repressions of 30% or more for 168 of 226 tested targets. The analysis suggests that some microRNAs may have as many as a few thousand targets, and that between 74% and 92% of the gene transcripts in four model genomes are likely under microRNA control through their untranslated and amino acid coding regions. We also extended the method's key idea to a low-error microRNA-precursordiscovery scheme; our studies suggest that the number of microRNA precursors in mammalian genomes likely ranges in the tens of thousands.
Citation for published version (APA): Abdel-Mageed, W. M., Lehri, B., Jarmusch, S. A., Miranda, K... more Citation for published version (APA): Abdel-Mageed, W. M., Lehri, B., Jarmusch, S. A., Miranda, K., Al-Wahaibi, L. H., Stewart, H. A., Jamieson, A. J., Jaspars, M., & Karlyshev, A. V. (2020). Whole genome sequencing of four bacterial strains from South Shetland Trench revealing biosynthetic and environmental adaptation gene clusters. Marine Genomics, 54, [100782]. https://doi.org/10.1016/j.margen.2020.100782
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Papers by Kevin Miranda