Papers by Katrina Jolliffe
Acta Crystallographica Section E-structure Reports Online, Dec 6, 2006
Acta Cryst. (2007). E63, o92-o93 Banister et al.¯C 11 H 14 O 2 organic papers o92 Banister et al.... more Acta Cryst. (2007). E63, o92-o93 Banister et al.¯C 11 H 14 O 2 organic papers o92 Banister et al. C 11 H 14 O 2
Journal of Cell Science, 2021
The lipid content of mammalian cells varies greatly between cell type. Current methods for analys... more The lipid content of mammalian cells varies greatly between cell type. Current methods for analysing lipid components of cells are technically challenging and destructive. Here, we report a facile, inexpensive method to identify lipid content – intracellular flow cytometric lipid analysis (IFCLA). Distinct lipid classes can be distinguished by Nile Blue fluorescence, Nile Red fluorescence or violet autofluorescence. Nile Blue is fluorescent in the presence of unsaturated fatty acids with a carbon chain length greater than 16. Cis-configured fatty acids induce greater Nile Blue fluorescence than their trans-configured counterparts. In contrast, Nile Red exhibits greatest fluorescence in the presence of cholesterol, cholesteryl esters, some triglycerides and phospholipids. Multiparametric spanning-tree progression analysis for density-normalized events (SPADE) analysis of hepatic cellular lipid distribution, including vitamin A autofluorescence, is presented. This flow cytometric syst...
Chemistry – A European Journal, 2020
Chemistry (Weinheim an der Bergstrasse, Germany), Jan 8, 2018
Fluorescent sensors that illuminate specific molecules and chemical events allow the selective an... more Fluorescent sensors that illuminate specific molecules and chemical events allow the selective and sensitive study of the cellular environment. At the centre of this technology lies the fluorescent reporter molecule, and it is therefore crucial to provide a breadth of fluorophores with varying photophysical and biological behaviour. 4-Amino-1,8-naphthalimides are commonly employed in fluorescent sensors, but the narrow range of structural derivatives limits versatility of application. Here we report the synthesis and investigation of a set of twelve 4-amino-1,8-naphthalimides bearing an additional substituent on the aromatic core. Photophysical characterisation and Time-Dependent Density Functional Theory studies provided insights into the structure-photophysical property relationships of these derivatives, which show an expanded range of emission wavelengths and other photophysical properties. These compounds could all be visualised within cells by confocal microscopy, showing cyto...
Australian Journal of Chemistry
ABSTRACT
Australian Journal of Chemistry, 2015
Two tripodal ligands, each derived from 1,1,1-tris(hydroxymethyl)ethane and terminated respective... more Two tripodal ligands, each derived from 1,1,1-tris(hydroxymethyl)ethane and terminated respectively by 4-pyridyl (L1) and 2-pyridyl groups (L2), have been synthesised. Competitive seven-metal extraction studies (H2O/CHCl3) incorporating equal concentrations of cobalt(ii), nickel(ii), copper(ii), zinc(ii), silver(i), cadmium(ii), and lead(ii) in the aqueous phase and L1 or L2 in the organic phase showed selective extraction of silver(i) in each case. A parallel solvent extraction experiment involving a related tripodal tris-pyridyl ligand (L3) based on a 1,3,5-substituted aryl ring scaffold and incorporating thioether sulfurs in each tripod arm also showed extraction selectivity for silver(i); extraction efficiencies towards this metal ion fall in the order L3 > L1 > L2. Physical data are in accord with L1 forming a capsule-like complex of type [Ag3L12]3+ in which silver ions link pairs of pyridyl groups from different ligands. In contrast, L2 yields a complex of type [Ag2L2(NO...
ChemInform, 2005
A range of 3-substituted-4,6-dimethoxyindoles bearing electron-withdrawing groups in either the 2... more A range of 3-substituted-4,6-dimethoxyindoles bearing electron-withdrawing groups in either the 2-or 7-position, can be nitrated using nitric acid adsorbed on silica, to give 7-nitro and 2-nitro-indoles, respectively. A 1-cyano-indole gives regioselectively the 2-nitroindole with loss of the cyano group.
Tetrahedron, 2004
ABSTRACT
Journal of the American Chemical Society, 1999
The Journal of Organic Chemistry, 2004
The cyclization of small peptides which do not incorporate turn inducers is often difficult. We h... more The cyclization of small peptides which do not incorporate turn inducers is often difficult. We have developed a method involving the use of removable turn inducers, in the form of pseudoprolines, for the cyclization of difficult peptide sequences. The pseudoprolines induce a cisoid amide bond in the peptide backbone which facilitates cyclization. They are then readily removed to yield a cyclic peptide that does not contain any turn inducers.
Journal of Medicinal Chemistry, 2006
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2011
... bis-b-diketones Jack K. Clegg Bianca Antonioli David J. Bray Karsten Gloe Kerstin Glo... more ... bis-b-diketones Jack K. Clegg Bianca Antonioli David J. Bray Karsten Gloe Kerstin Gloe Katrina A. Jolliffe Olga N. Kataeva George V. Meehan Marco Wenzel Received: 9 March 2011 / Accepted: 21 March 2011 Ó Springer Science+Business Media BV 2011 ...
Journal of Antimicrobial Chemotherapy, 2013
Antifungal treatment of uncommon filamentous fungal infections is problematic. This study determi... more Antifungal treatment of uncommon filamentous fungal infections is problematic. This study determined the in vitro susceptibility of miltefosine, as a single agent and in combination with posaconazole or voriconazole, against these pathogens. Methods: Susceptibility to miltefosine of 34 uncommon filamentous fungi was tested using CLSI broth microdilution M38-A2 methodology. Twenty isolates were studied for potential synergy using miltefosine/posaconazole and miltefosine/voriconazole combinations and the chequerboard microdilution assay. Results: MICs of miltefosine were high (in general, .8 mg/L) for most isolates compared with amphotericin B, echinocandins and the azoles. Miltefosine had greatest activity against Scedosporium spp., Lichtheimia corymbifera and Rhizomucor sp. (MICs ≤4 mg/L). Miltefosine in combination either with posaconazole or voriconazole demonstrated synergy [fractional inhibitory concentration index (FICI) ≤0.5] in 12 instances (11 isolates): miltefosine/ posaconazole combinations were synergistic against 3 of 4 Fusarium oxysporum strains (FICI range 0.37-0.5) and 5 of 10 mucormycete strains (FICI range 0.06-0.5). The combination of voriconazole with miltefosine showed synergy against one Scedosporium prolificans isolate and three mucormycetes-a single strain each of L. corymbifera, Rhizopus oryzae and Rhizomucor sp. No antagonism was observed. Conclusions: Miltefosine demonstrated synergy in 8/20 (40%) and 4/20 (20%) instances when combined with posaconazole and voriconazole, respectively. Synergy was most often observed against F. oxysporum and the mucormycetes. Study of miltefosine/azole combinations as a novel antifungal approach is indicated.
Fungal Genetics and Biology, 2014
Miltefosine (MI) is a novel, potential antifungal agent with activity against some yeast and fila... more Miltefosine (MI) is a novel, potential antifungal agent with activity against some yeast and filamentous fungal pathogens. We previously demonstrated in the model yeast, Saccharomyces cerevisiae, that MI causes disruption of mitochondrial membrane potential and apoptosis-like cell death via interaction with the Cox9p sub-unit of cytochrome c oxidase (COX). To identify additional mechanisms of antifungal action, MI resistance was induced in S. cerevisiae by exposure to the mutagen, ethyl methanesulfonate, and gene mutation(s) responsible for resistance were investigated. An MI-resistant haploid strain (H-C101) was created. Resistance was retained in the diploid strain (D-C101) following mating, confirming dominant inheritance. Phenotypic assessment of individual D-C101 tetrads revealed that only one mutant gene contributed to the MI-resistance phenotype. To identify this gene, the genome of H-C101 was sequenced and 17 mutated genes, including metacaspase-encoding MCA1, were identified. The MCA1 mutation resulted in substitution of asparagine (N) with aspartic acid (D) at position 164 (MCA1 N164D). MI resistance was found to be primarily due to MCA1 N164D , as single-copy episomal expression of MCA1 N164D , but not two other mutated genes (FAS1 T1417I and BCK2 T104A), resulted in MI resistance in the wild-type strain. Furthermore, an MCA1 deletion mutant (mca1D) was MI-resistant. MI treatment led to accumulation of reactive oxygen species (ROS) in MI-resistant (MCA1 N164D-expressing and mca1D) strains and MI-susceptible (MCA1-expressing) strains, but failed to activate Mca1 in the MI-resistant strains, demonstrating that ROS accumulation does not contribute to the fungicidal effect of MI. In conclusion, functional disruption of Mca1, leads to MI resistance and inability to mediate MI-induced apoptotic effects. Mca1-mediated apoptosis is therefore a major mechanism of MI-induced antifungal action.
Fungal Genetics and Biology, 2013
Antimicrobial Agents and Chemotherapy, 2010
Ten bis(alkylpyridinium)alkane compounds were tested for antifungal activity against 19 species (... more Ten bis(alkylpyridinium)alkane compounds were tested for antifungal activity against 19 species (26 isolates) of yeasts and molds. We then determined the MICs and minimum fungicidal concentrations (MFCs) of four of the most active compounds (compounds 1, 4, 5, and 8) against 80 Candida and 20 cryptococcal isolates, in comparison with the MICs of amphotericin B, fluconazole, itraconazole, voriconazole, posaconazole, and caspofungin, using Clinical Laboratory and Standards Institutes broth microdulition M27-A3 (yeasts) or M38-A2 (filamentous fungi) susceptibility protocols. The compounds were more potent against Candida and Cryptococcus spp. (MIC range, 0.74 to 27.9 μg/ml) than molds (0.74 to 59.7 μg/ml). MICs against Exophiala were 0.37 to 5.9 μg/ml and as low as 1.48 μg/ml for Scedosporium but ≥25 μg/ml for zygomycetes, Aspergillus , and Fusarium spp. Compounds 1, 4, 5, and 8 exhibited good fungicidal activity against Candida and Cryptococcus , except for Candida parapsilosis (MICs ...
Antimicrobial Agents and Chemotherapy, 2007
The susceptibilities of 77 dermatophytes to miltefosine (MI), 1,12-bis(4-pentylpyridinium)dodecan... more The susceptibilities of 77 dermatophytes to miltefosine (MI), 1,12-bis(4-pentylpyridinium)dodecane (PYR), 1,12-bis(tributylammonium)dodecane (AM), itraconazole (ITC), terbinafine (TRB), and butenafine (BTF) were compared. Geometric mean MICs of TRB, BTF, ITC, MI, PYR, and AM were 0.039, 0.059, 1.718, 0.671, 6.006, and 4.771 μg/ml, respectively. MI was more active than ITC ( P < 0.001).
Angewandte Chemie International Edition, 1998
The high affinity of Ag ions for aromatic π donors and cyano groups is exploited in a novel MALDI... more The high affinity of Ag ions for aromatic π donors and cyano groups is exploited in a novel MALDI-TOF mass spectrometric method for the identification of hydrogen-bonded assemblies. The interaction with the Ag ions-which, for example, can be complexed by two phenyl groups in a sandwich-type manner (see drawing on the right)-provides positively charged assemblies in a nondestructive way.
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Papers by Katrina Jolliffe