Papers by KANIA ARDHANI PUTRI
Dentino Jurnal Kedokteran Gigi, Sep 6, 2019
Background: Haruan fish scales (Channa striata) contain chitin which can be used as antibacterial... more Background: Haruan fish scales (Channa striata) contain chitin which can be used as antibacterial ingredients by processing it into chitosan. Chitosan contains an amino polysaccharide group which can inhibit the growth of Staphylococcus aureus, one of the opportunistic bacteria, which becomes a pathogen if there is an imbalance within oral environment and one of the causes of periodontal disease. Besides, these bacteria are also reported to be resistant to conventional antibacterial agents. Objective: The purpose of this study was to find out and elaborate the difference in antibacterial activity of chitosan from haruan fish scales (Channa striata) at the concentration of 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus. Methods: This study used a randomized pretest-posttest with control group design and One Way ANOVA statistical test was performed and was followed by Post Hoc Dunnet test. The procedure of this study consisted of chitosan fabrication scales from fresh fish by deproteination, demineralization, and deacetylation, followed by an antibacterial test with 10 treatment groups namely 0.5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus to obtain Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: The results of this study presented chitosan at 1.5% concentration as the Minimum Inhibitory Concentration (MIC) and 3.5% concentration as the Minimum Bactericidal Concentration (MBC) on the growth of Staphylococcus aureus. Evaluated from the difference in absorbance, 2.5% chitosan concentration showed superior activity in inhibiting Staphylococcus aureus compared to 0.2% chlorhexidine gluconate. Conclusion: It can be concluded that there are differences in the antibacterial activity of chitosan scales (Channa striata) with a concentration of 0, 5%; 1%; 1.5%; 2%; 2.5%; 3%; 3.5%; 4% on the growth of Staphylococcus aureus.
Dentino Jurnal Kedokteran Gigi, Mar 7, 2018
Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 he... more Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 hectares or 90% of the area classified as peatland water. Society uses peatlands water with acidic pH(3.5-4.5) for everyday purposes including rinsing and brushing teeth. The acidic nature of peatland water can affect the growth of bacterial colonies in the oral cavity so that the acidic conditions of the oral cavity increases and the effect on the occurrence of dental caries. In addition PDAM water is water from river water that is processed through several stages to become clean water after filtration and disinfection stages to eliminate microorganisms. Objective: this study aims to determine the characterization of the number of colonies of aerobic bacteria of the oral cavity on peatland water and PDAM water. Methods: This study used quasi experimental method with post test only control group design. The study sample consisted of 30 respondents. Research materials were 4ml of a result of the salivary gargle on peatland water and PDAM water then the number of aerobic bacteria colonies were calculated by the method of TPC (Total Plate Count) and characterization of aerobic bacteria was done by using catalase test, test MSA and coagulase test. Results: This study showed that there were 1653 CFU/ml of aerobic aerobic colony, whereas in water of PDAM there were 1264 CFU/ml of aerobic colonies. In the characterization tests of aerobic bacteria colonies showed that there were Staphylococcus aureus and Staphylococcus epidermidis bacteria on gargle water of peatland or PDAM water. Independent t-test results showed significant differences in aerobic bacterial colonies in peatland water and PDAM water (p = 0.001)(p = 0.002)(p <0.05). Conclusion: Based on this study it can be concluded that the number of Staphylococcus aureus and Staphylococcus epidermidis found in the oral cavity of children saliva who gargled with peatland water are higher than the number of Stapylococcus aureus and Staphylococcus epidermidis found in the saliva of children who gargle with PDAM water.
Dentino Jurnal Kedokteran Gigi, May 13, 2016
Background: Acidic drinks that cause erosion of the teeth. If the acid continue sticking to the t... more Background: Acidic drinks that cause erosion of the teeth. If the acid continue sticking to the teeth it will decrease the surface hardness of tooth. The demineralization of the tooth surface cause the formation of porosity. Purpose: This study aimed to determine the differences in surface hardness of tooth due to duration of immersion with orange juice in vitro. Methods: This research design was a true experimental research postest only with control group design. The sample was consisted of 4 treatment groups, one group of teeth that was not immersedwith orange juice as a positive control group and 3 another groups teeth immersedwith orange juice for 30, 60 and 120 minutes. Each tooth surface hardness was measured using the Micro Vicker Hardness Tester. Result: The average hardness of the tooth surface that was not immersed orange juice 372.8 VHN, immersedin orange juice for 30 minutes 323.7 VHN, 60 minutes 292.5 VHN, 120 minutes 259.1 VHN. One way ANOVA test showed significant differences in the surface hardness of the tooth between 2 groups. Conclusion: The longer of immersed time may effect in enamel surface hardness decreases.
Majalah Obat Tradisional, 2021
Use and demand of traditional medicinal plants currently growing in the direction that research i... more Use and demand of traditional medicinal plants currently growing in the direction that research in traditional medicines have also increased. One of the plants that is efficacious as a medicine is S. jamaecensis. This plant is usually used by people as a traditional medicine to treat various diseases. This study aims to determine the antioxidant activity of porterweed root. Antioxidant activity assays performed using hydroxyl radical scavenger that begins with the extraction by maceration. Dry roots of S. jamaecensis  (250 g) was soaked with ethanol 96% for 3x24 hours at room temperature. The liquid extract obtained is evaporated by rotary evaporator and waterbath till viscous extracts is obtained. Then to extract performed phytochemical screening by quantitative and qualitative method. The antioxidant test was carried out using the hydroxyl radical scavenger method. The extract concentrations were varied, namely 125, 250, 500 and 1000 ppm. The data obtained was performed one-way AN...
Background: The most often disease that occurs in the oral cavity is Periodontitis and one of tha... more Background: The most often disease that occurs in the oral cavity is Periodontitis and one of that is chronic periodontitis type. The dominant bacteria in chronic periodontitis with 96,2% of prevalence is Porphyromonas gingivalis . Supporting treatment for chronic periodontitis is mouthwash administration, but in long term use may causing some side effect, so that an alternative antibacterial agent with minimal side effects is needed. One of the plants that have antibacterial compounds is the Kasturi plant typical of South Kalimantan, because contain tannins, terpenoids, flavonoids and phenolics. Purpose: Determine and analyzing the antibacterial effectiveness of Kasturi leaf extract ( Mangifera casturi ) against the growth of Porphyromonas gingivalis bacteria. Methods: This study was used True Experimental with posttest only control group and used the broth dilution method to determine MIC and agar dilution to determine the MBC. Results: The leaves extract of Kasturi has a Minimum ...
Systematic Reviews in Pharmacy, 2020
Background: Haruan fish is one of the fisheries production which is quite popular in South Kalima... more Background: Haruan fish is one of the fisheries production which is quite popular in South Kalimantan. The body parts of the fish that usually become waste are scales. The lack of management of these wastes raises various issues in the environmental field that can extend to social and health problems, whereas fish scales contain chitin which can be synthesized into chitosan which is useful in the biomedical field. However, chitosan scales (Channa striata) have never been used optimally because there are no data available regarding the content of chitosan scales. Purpose:: The purpose of this study is to identify and know the characteristics of chitosan contained in the scales of haruan (Channa striata). Methods and materials: This research used a descriptive observational method by conducting quantitative analysis to determine physical characteristics (yield, moisture content, ash content, protein content, fat content, fiber content, carbohydrate content), chemical characteristics (...
Manggis (Garcinia mangostana L.) merupakan tanaman tahunan dan tanaman asli asia tenggara. Kulit ... more Manggis (Garcinia mangostana L.) merupakan tanaman tahunan dan tanaman asli asia tenggara. Kulit dari buah manggis mengandung pigmen dari kelompok antosianin dengan kemampuan sebagai antioksidan yang disebut dengan senyawa xanthone. Salah satu bentuk alternatif pemanfaatan hasil kulit manggis adalah sebagai campuran dalam produk es krim. Serbuk kulit manggis dibuat dengan beberapa tahapan yaitu kulit manggis dicuci dan dipotong kecil-kecil untuk selanjutnya dikeringkan di bawah paparan sinar matahari selama ± 3 hari. Setelah itu kulit manggis kering dihancurkan menggunakan blender dan diayak sehingga lolos ayakan berukuran 80 mesh. Serbuk kulit manggis kemudian diolah menjadi produk es krim. Pembuatan es krim kulit manggis dilakukan dengan mencampurkan 210 gram serbuk kulit manggis, 600 gram susu cair, 200 gram susu kental manis, 45 gram tepung maizena, 25 gram pengemulsi, 45 gram gula pasir menggunakan dimixer selama 15 menit hingga menjadi adonan es krim. Strawberry yang telah dib...
Dentino : Jurnal Kedokteran Gigi, 2020
ABSTRACTBackground: Karamunting leaf extract has secondary metabolite compounds such as tannin, f... more ABSTRACTBackground: Karamunting leaf extract has secondary metabolite compounds such as tannin, flavonoid, and phenol that have antibacteria potential to be used as an alternative to mouthwash in addition to Chlorhexidine 0.12% to lower the incidence of tooth caries. Caries is caused by several important virulence factors, including Streptococcus mutans Glucosyltransferase (GTF) enzyme. Karamunting leaf extract can inhibit the work of GTF enzyme by precipitating, denaturing protein and damaging cell walls. Inhibition of Streptococcus bacteria enzyme activity will decrease plaque formation, thus decreasing the potential of dental caries. Objective: The purpose of this study was to determine the effect of Karamunting leaf extract and 0,12% Chlorhexidine on Glucosyltransferase enzyme activity of Streptococcus mutans. Methods: This study used true experimental design with post test only with control group design using 7 groups, including Karamunting leaf extract group with 5%, 10%, 15%,...
Dentino : Jurnal Kedokteran Gigi, 2020
ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will ... more ABSTRACTBackground: Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Methods: This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration wi...
International Journal of Research in Pharmaceutical Sciences, 2020
Channa micropeltes is a local peatland water species which has been utilized as an alternative of... more Channa micropeltes is a local peatland water species which has been utilized as an alternative of Channa striata among South Kalimantan population to anticipate the scarcity of Haruan species. Channa micropeltes originate from the same family of Channa striata in which both fishes contain albumin. Albumin is proven to accelerate wound healing, thus can be produced as capsules. The capsulate form of Channa micropeltes extract will later be deployed as an alternative herbal medicine for the acceleration of mucosal wound healing. Nevertheless, a study of a hepatological profile is pivotal to assess the safety of its consumption. The present study aimed to analyze the effect of Channa micropeltes extracts capsulate oral administration at 0.7 g dosage on SGOT and SGPT level of Wistar rat liver. This was a real experimental study with post-test only and control group design. The samples comprised of 12 rats which were distributed into three groups namely a treatment group of 0.7 g Channa ...
Dentino : Jurnal Kedokteran Gigi, 2020
Background: Periodontal disease is one of the dental and oral diseases that is often found in peo... more Background: Periodontal disease is one of the dental and oral diseases that is often found in people in Indonesia. RISKESDAS (2018) stated that the prevalence of people who have dental and oral health problems in Indonesia is 57.6%. The prevalence of oral and dental problems in South Kalimantan is 60%. Periodontal disease is damage to the supporting tissues of the teeth caused by specific microorganisms such as Porphyromonas gingivalis which are gram negative bacteria. The growth of these bacteria can be inhibited and killed by chitosan from haruan fish scales. Chitosan is a natural product of chitin, chitosan is the basic ingredient of fish scales. Chitosan has advantages and more safety levels, because it has an active group that will bind to microbes so chitosan can inhibit and kill microbial growth. Objective: to analyze the antibacterial activity of chitosan scales (Channa striata) on the growth of Porphyromonas gingivalis. Methods: This research used true experimental with ran...
BIO Web of Conferences, 2020
Antibacterial compounds are generally produced from many herbal ingredients, but antibacterials m... more Antibacterial compounds are generally produced from many herbal ingredients, but antibacterials may be produced from soil microbes, one of which is Actinomycetes. Actinomycetes are a soil bacterium capable of producing secondary metabolites in the form of antibacterial compounds. The antibacterial compound has the potential as an alternative antibacterial agent against oral microbes. One of the oral microbes that have a major role in the occurrence of caries isStreptococcus mutansandLactobacillus acidophilus. This study aimed to analyze the antibacterial activity of Actinomycetes against the growth ofS. mutansandL. acidophilusActinomycetes extracts at the concentration of 0.625%, 1.25%, 2.5%, 5%, 10%, 20%, and 40% were respectively exerted as the treatment groups while 0.12% Chlorhexidine Gluconate (CHX) and aquadest as control. The measurement of antibacterial activity is done by looking at MIC and MBC. One Way ANOVA and Post Hoc Dunnets T3 test MIC and MBC values show p=0,000 (p &...
Dental Journal (Majalah Kedokteran Gigi), 2018
Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxid... more Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl ace...
Dental Journal (Majalah Kedokteran Gigi), 2016
Background: Dental caries is a major problem in oral cavity. If dental caries causes cavity, the ... more Background: Dental caries is a major problem in oral cavity. If dental caries causes cavity, the structure of dental hard tissue will not be reversible because of damage in the structure of the hard tissue. The early pathogenesis mechanism of dental caries is an adhesion interaction between cariogenic Streptococcus mutans microorganisms and tooth surface pellicles. The attachment involves a specific molecular component interaction between the bacterial complement molecules and the surface of the host. Streptococcus sanguis as a dominant ecology at the beginning of bacterial plaque aggregation will colonize the tooth surface earlier than S. mutans. The surface of bacterial cells can express some adesin. The bacteria also can express receptors for adhesins of other bacteria. Specific receptors for adhesions of S. Mutans bacteria are not only found in the pellicles, but also present in pioneer bacteria, such as S. sanguis. Adhesion between those bacteria is called as coagregation. Purp...
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Papers by KANIA ARDHANI PUTRI