Turnip crinkle virus (TCV) has been implicated in the suppression of the hypersensitive response ... more Turnip crinkle virus (TCV) has been implicated in the suppression of the hypersensitive response (HR), a type of programmed cell death induced during active resistance in Arabidopsis thaliana. In order to investigate the involvement of individual viral components in mediating suppression, TCV genes were cloned for use in an Agrobacterium tumefaciens mediated transient expression in Nicotiana benthamiana. Agroinfiltration of the HR-inducing avrPto/Pto system in conjunction with individual TCV genes has identified the p38 gene, which encodes the viral coat protein, as the gene responsible for the cell death suppression phenotype. The extent of cell death suppression by coat protein was quantified and found to be equal to the level of suppression by the whole virus and AvrPtoB, another cell death inhibitor from bacteria. Thus, the coat protein alone is sufficient to inhibit the HR in plants. Further, the effect of TCV on HR initiation by an avirulence factor from an unrelated bacterial pathogen was investigated. The presence of TCV does not affect the production, secretion or cellular processing of the bacterial avirulence factor.
The objective of this study was to identify the suitable gelling agent and size of nodal segment ... more The objective of this study was to identify the suitable gelling agent and size of nodal segment in order to enhance in vitro multiplication rate of different Indian potato genotypes. The results showed that MS medium solidified with agar increased the number of leaves, nodes and roots as well as root length, whereas phytagel enhanced the microplant height, internodal length, fresh and dry weight of microplants of potato. Media solidified with agar enhanced the in vitro multiplication rate in Kufri Bahar,
Turnip crinkle virus (TCV) has been implicated in the suppression of the hypersensitive response ... more Turnip crinkle virus (TCV) has been implicated in the suppression of the hypersensitive response (HR), a type of programmed cell death induced during active resistance in Arabidopsis thaliana. In order to investigate the involvement of individual viral components in mediating suppression, TCV genes were cloned for use in an Agrobacterium tumefaciens mediated transient expression in Nicotiana benthamiana. Agroinfiltration of the HR-inducing avrPto/Pto system in conjunction with individual TCV genes has identified the p38 gene, which encodes the viral coat protein, as the gene responsible for the cell death suppression phenotype. The extent of cell death suppression by coat protein was quantified and found to be equal to the level of suppression by the whole virus and AvrPtoB, another cell death inhibitor from bacteria. Thus, the coat protein alone is sufficient to inhibit the HR in plants. Further, the effect of TCV on HR initiation by an avirulence factor from an unrelated bacterial pathogen was investigated. The presence of TCV does not affect the production, secretion or cellular processing of the bacterial avirulence factor.
The objective of this study was to identify the suitable gelling agent and size of nodal segment ... more The objective of this study was to identify the suitable gelling agent and size of nodal segment in order to enhance in vitro multiplication rate of different Indian potato genotypes. The results showed that MS medium solidified with agar increased the number of leaves, nodes and roots as well as root length, whereas phytagel enhanced the microplant height, internodal length, fresh and dry weight of microplants of potato. Media solidified with agar enhanced the in vitro multiplication rate in Kufri Bahar,
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