I am now a retiree. My intetest is molecular genetics, pharmaceutical and pediatrics. Most of my time are spending in my home. I can translate medical literatures.
Brown clear fluid, slightly fragrant, taste sweet, slightly bitter. [Indications]Filling kidney a... more Brown clear fluid, slightly fragrant, taste sweet, slightly bitter. [Indications]Filling kidney and growing essence. Develop yin and help yang.Use for ache of waist and knee, swirl and tingle, physically and mentally fatigue, insomnia and forgettery, chilly and cold extremities, frequent nocturia and numbness of extremities caused by lack of kidney vital energy and lack of kidney essence.
Brown clear fluid, slightly fragrant, taste sweet, slightly bitter. [Indications]Filling kidney a... more Brown clear fluid, slightly fragrant, taste sweet, slightly bitter. [Indications]Filling kidney and growing essence. Develop yin and help yang.Use for ache of waist and knee, swirl and tingle, physically and mentally fatigue, insomnia and forgettery, chilly and cold extremities, frequent nocturia and numbness of extremities caused by lack of kidney vital energy and lack of kidney essence.
As far as we know neonatal septicemia caused by coagulase negative Staphylococcus hemolyticus ha... more As far as we know neonatal septicemia caused by coagulase negative Staphylococcus hemolyticus has not been reported in literature (14). This paper presents 3 such cases and the application of plasmid analysis antibiograms and phage typing for epidemiological analysis.
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方法 培养新生大鼠脑的微血管内皮，星型小胶质和平滑肌细胞，8-异- PGF2α的细胞毒性通过MTT分析和乳酸脱氢酶释放，propidium碘混合和DNA片段化（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记[TUNEL]）来测定。另外，确定了室内注射8-异- PGF2α的效果和8-异- PGF2α诱导的细胞毒性可能涉及到血栓烷。
结果 8-异- PGF2α诱导了时间和浓度依赖的内皮细胞死亡（EC50=0.1nmol/L）,但对平滑肌和星型小胶质细胞几乎不起作用。内皮细胞死亡绝大多数死于膨胀性质（propidium碘混合和乳酸脱氢酶释放），细胞死亡与内皮血栓烷A2（TXA2）形成增加有关并可被TXA2合成酶抑制剂（CGS12970和U63557A）所预防；TXA2类似物U46619和I-BOP也引起内皮细胞死亡。室内注射8-异- PGF2α诱导室周损伤，但可被CGS12970减弱。
结论 这些资料揭示了8-异- PGF2α在氧化剂所诱导的脑微血管损伤和大脑损害中的新作用。这些作用涉及TXA2。
（Stroke 2003;34:776-782）
关键词：细胞凋亡，脑损伤，坏死，氧化剂，血栓烷，白质
方法 培养新生大鼠脑的微血管内皮，星型小胶质和平滑肌细胞，8-异- PGF2α的细胞毒性通过MTT分析和乳酸脱氢酶释放，propidium碘混合和DNA片段化（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记[TUNEL]）来测定。另外，确定了室内注射8-异- PGF2α的效果和8-异- PGF2α诱导的细胞毒性可能涉及到血栓烷。
结果 8-异- PGF2α诱导了时间和浓度依赖的内皮细胞死亡（EC50=0.1nmol/L）,但对平滑肌和星型小胶质细胞几乎不起作用。内皮细胞死亡绝大多数死于膨胀性质（propidium碘混合和乳酸脱氢酶释放），细胞死亡与内皮血栓烷A2（TXA2）形成增加有关并可被TXA2合成酶抑制剂（CGS12970和U63557A）所预防；TXA2类似物U46619和I-BOP也引起内皮细胞死亡。室内注射8-异- PGF2α诱导室周损伤，但可被CGS12970减弱。
结论 这些资料揭示了8-异- PGF2α在氧化剂所诱导的脑微血管损伤和大脑损害中的新作用。这些作用涉及TXA2。
（Stroke 2003;34:776-782）
关键词：细胞凋亡，脑损伤，坏死，氧化剂，血栓烷，白质
application of plasmid analysis antibiograms and phage typing for epidemiological analysis.