Papers by Jovana Grahovac
Contemporary Agriculture, Jun 1, 2022
The use of plant protection products (PPPs) has become a social concern regarding food safety and... more The use of plant protection products (PPPs) has become a social concern regarding food safety and quality, and risks to human health and the environment. Accordingly, the agricultural microbials market is a steadily growing sector of the global agriculture industry. This paper evaluates the market of basic groups of pesticides in Serbia (namely herbicides, fungicides and insecticides), as well as other collectively presented plant protection products, with a particular emphasis on the biopesticides segment. The official data of the Serbian Plant Protection Directorate for the period 2000-2020 served as a primary source of data. The movement of PPPs and active ingredients (AIs) imports into Serbia were analyzed separately. The average values of PPP quantities imported into Serbia were computed for the period under consideration. The time series variability was evaluated using the coefficient of variation, whereas the dynamics of PPP import movements was evaluated using the rate of change. In the overall structure of the imported pesticides in Serbia, herbicides remain the dominant product type (57.0%), followed by fungicides (27.5%), insecticides (10.5%), and other PPPs (5.0%). The import of AIs for the production of herbicides claimed a share of 79.8%, whereas the share of AIs for the fungicide production approximated to 12.8% (followed by AIs for the insecticide production (6.7%) and AIs for the other groups of pesticides (0.7%). Biopesticides comprise a small share of the total crop protection market in Serbia. Bioinsecticides were found to be imported into Serbia in largest quantities (followed by biofungicides, bioacaricides, and biobactericides). No imports of bioherbicides have been recorded. According to the official data on the import of PPPs in Serbia in the period 2000-2020, it can be concluded that there is a positive growth trend, which is expected to continue in the future. Although the import of AIs shows a positive rate of change, no significant growth trends were recorded.
Antibiotics, Mar 15, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Chemical Technology & Biotechnology, Dec 23, 2019
BACKGROUND Popularity of biocontrol has increased due to the growing environmental concerns and t... more BACKGROUND Popularity of biocontrol has increased due to the growing environmental concerns and the demand for organic products. It is considered as a sustainable practice for efficient agricultural production. Microbial biopesticides offer environment-friendly biotechnological alternative to chemical control of plant diseases and pests. Due to its advantages in cell separation cross-flow microfiltration has emerged as a powerful alternative to the conventional separation methods. However, the flux enhancement remains to be addressed. This study has estimated the use of flux improvement techniques for biomass harvesting by This article is protected by copyright. All rights reserved. microfiltration using tubular ceramic membranes. Investigated methods for flux enhancement were turbulence promoter i.e. Kenics static mixer, two-phase flow (air sparging) and the combination of these two methods. RESULTS Significant flux improvement coincides with a reduction in specific energy consumption, while at the same time Bacillus velezensis cells have retained viability and antibacterial activity. The highest values of flux and corresponding improvement, compared to the microfiltration without enhancement techniques, are obtained in the case of combining Kenics static mixer and two-phase flow (133.4 L/m 2 h and 502%). Flux improvement for air sparging was 134%, for turbulence promoter alone flux increase was 434%. CONCLUSION The methods proposed herein have achieved effective flux enhancement for cell harvesting. The obtained results also suggest encouraging basis to promote large scale economical production of biomass for biocontrol application.
Polymers, Aug 12, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Micromachines, Feb 28, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Environmental Management, Dec 1, 2017
In this research a process and cost model for a xanthan production facility was developed using p... more In this research a process and cost model for a xanthan production facility was developed using process simulation software (SuperPro Designer ®). This work represents a novelty in the field for two reasons. One is that xanthan gum has been produced from several wastes but never from wastewaters from confectionery industries. The other more important is that the aforementioned software, which in intended exclusively for bioprocesses, is used for generating a base case, i.e. starting point for transferring the technology to industrial scales. Previously acquired experimental knowledge about using confectionery wastewaters from five different factories as substitutes for commercially used cultivation medium have been incorporated into the process model in order to obtain an economic viability of implementing such substrates. A lower initial sugar content in the medium based on wastewater (28.41 g/L) compared to the synthetic medium (30.00 g/L) gave a lower xanthan content at the end of cultivation (23.98 and 26.27 g/L, respectively). Although this resulted in somewhat poorer economic parameters, they were still in the range of being an investment of interest. Also the possibility of utilizing a cheap resource (waste) and reducing pollution that would result from its disposal has a positive effect on the environment.
Journal on Processing and Energy in Agriculture, 2023
Intensive food production to feed the growing human population requires significant material and ... more Intensive food production to feed the growing human population requires significant material and energy resources, simultaneously generating large amounts of waste, whose treatment adds an additional cost to the overall production process. Wastewaters arising from meat and dairy processing industries contain large amounts of organic and inorganic nutrients, which present a heavy environmental burden, but, on the other hand, could serve as nutrient sources for microbial growth. The aim of this study was to investigate the suitability of meat processing wastewater and whey from the dairy industry as media for the growth of biocontrol strain Bacillus velezensis IP22. Both waste-based media have contributed to antibacterial and antifungal activity to a similar degree, with highly comparable inhibition zone diameters against Xanthomonas campestris, Xanthomonas euvesicatoria and Aspergillus flavus. Furthermore, biocontrol activity of the cultivation broths obtained using the waste-based media was slightly lower compared to the nutrient broth medium, suggesting the possibility to replace the expensive chemically defined medium with a complex medium alternative. This approach based on circular economy principles promotes responsible resource utilization through the reuse of food industry effluents for the production of value value-added products, opening a chapter of possibilities to establish an industrial symbiosis network between companies in different industry branches.
Journal on Processing and Energy in Agriculture, 2018
The bioethanol market is large and has a constant upward trend, which requires the improvement of... more The bioethanol market is large and has a constant upward trend, which requires the improvement of its production. In the Republic of Serbia, bioethanol is produced using sugar beet molasses-based media. Taking into account a few disadvantages of molasses as raw material, as well as a decreasing trend in its use in Serbia, it is necessary to consider other possibilities provided by the sugar beet processing technology. Therefore, the cogeneration of sugar and bioethanol may serve as an alternative and viable solution. In this paper, the data on the characteristics of intermediate and by-products of sugar beet processing, used as raw materials for bioethanol production, are summarized. The criteria for their selection are given, and the locations of their generation are mapped. Furthermore, the current bioethanol production capacities in the Republic of Serbia are presented, and the possibility of the cogeneration of sugar and bioethanol in domestic factories is considered.
Processes, Apr 4, 2023
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Croatica Chemica Acta, 2021
Groundwater with increased ammonia concentration is a constant concern regarding the preparation ... more Groundwater with increased ammonia concentration is a constant concern regarding the preparation of drinking water. The affinity of ammonia to be adsorbed on the surface of different solid materials significantly influences the selection of its removal process and has been the motivation for this investigation. Crystal-Right™ (CR-100) was used for the removal of ammonia from aqueous solution in batch adsorption procedure. The kinetics of adsorption followed the pseudo-second-order model. The Elovich model suggested that chemisorption rate decreased with the temperature increase. The liquid film diffusion and intra-particle diffusion models revealed that heterogeneous adsorbent surface energy had a particularly pronounced impact on the overall mass transfer rate. The Arrhenius and Eyring's equations suggested spontaneous and endothermic nature of complex adsorption/ion exchange removal process. The isosteric heat of adsorption revealed that with the increase in surface loading lateral interactions between the adsorbed molecules occurred.
Membranes, Jul 4, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of engineering & processing management, Dec 25, 2017
Biopolymers have the ability to absorb large amounts of water or biological fluids. They are swel... more Biopolymers have the ability to absorb large amounts of water or biological fluids. They are swelling and forming hydrogels. Thanks to this, they have found a variety of applications, and among others they can be used to remove some metal ions from the water. Due to its unique structure and exceptional rheological characteristics, microbiological biopolymer, xanthan, can also be used for purification of wastewater because structures formed in the presence of metals can be easily separated.Therefore, the research from this paper was carried out in order to examine the possibilities of crosslinking the xanthan with different metal ions. Based on the successful crosslinking and stability of the formed crosslinked structures, it has been estimated that with the use of this biopolymer it is possible to remove ions of lead and iron from wastewaters.
Bioengineering, Dec 13, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Acta Periodica Technologica, 2017
In the effort to overcome the increase in antimicrobial resistance of different pathogens, natura... more In the effort to overcome the increase in antimicrobial resistance of different pathogens, natural products from microbial sources appear to be the most favorable alternative to current antibiotics. Production of antimicrobial compounds is highly dependent on the nutritional conditions. Hence, in order to achieve high product yields, selection of the media constituents and optimization of their concentrations are required. In this research, the possibility of antimicrobial substances production using Bacillus subtilis ATCC 6633 was investigated. Also, optimization of the cultivation medium composition in terms of contents of glycerol, sodium nitrite and phosphates was done. Response surface methodology and the method of desirability function were applied for determination of optimal values of the examined factors. The developed model predicts that the maximum inhibition zone diameters for Bacillus cereus ATCC 10876 (33.50 mm) and Pseudomonas aeruginosa ATCC 27853 (12.00 mm) are achieved when the initial contents of glycerol, sodium nitrite and phosphates were 43.72 g/L, 1.93 g/L and 5.64 g/L, respectively. The results of these experiments suggest that further research should include the utilization of crude glycerol as a carbon source and optimization of composition of such media and cultivation conditions in order to improve production of antimicrobial substances using Bacillus subtilis ATCC 6633.
Analecta, May 12, 2014
Xanthan gum is one of the major commercial biopolymers employed in many industrial processes owin... more Xanthan gum is one of the major commercial biopolymers employed in many industrial processes owing to its unique physical properties such as a high degree of pseudoplasticity and high viscosity even at low concentrations. Commercially available xanthan gum is relatively expensive due to glucose or sucrose being used as the sole carbon source for its production and cost reduction could be achieved by using less expensive substrates, such as food industrial wastewaters. Effluents from the confectionery industry, because of its high organic content, are significant environmental pollutants and before their release into environment it is necessary to purify them. The present study examines xanthan production by Xanthomonas campestris under aerobic conditions on wastewaters from five different factories of the confectionery industry. Xanthan yield was obtained as a quantitative characteristic of the process and was in the range between 4.28 g/L and 10.03 g/L and its quality is determined by following rheological characteristics of obtained cultivation media. The results obtained in this study indicate that wastewater from confectionary industry can be used as the basis of media for the production of this highly valuable product.
Journal of Biotechnology, May 1, 2022
The microbiologically induced calcite precipitation (MICP) can be an emerging approach that could... more The microbiologically induced calcite precipitation (MICP) can be an emerging approach that could tap onto soil bacterial diversity and use as a bioremediation technique. Based on the concept that bacteria with biomineralization capacity could be effective CaCO3 inductance agents, this study aimed to evaluate the simultaneous influence of 11 operational and environmental factors on the MICP process, for the first time. Therefore, Bacillus muralis, B. lentus, B. simplex, B. firmus, and B. licheniformis, isolated from alkaline soils, were used in the selection of the best performing bacterium compared with a well-known MICP bioagent Sporosarcina pasteurii DSM 33. Plackett-Burman's experimental design was labouring to screen all independent variables for their significances on five outputs (pH value, number of viable cells and spores, amount of urea and CaCO3 precipitate). According to experimentally obtained data, an artificial neural network model based on the Broyden-Fletcher-Goldfarb-Shanno algorithm showed good prediction capabilities, while differences in the relative influences were observed at the bacterial strain level. B. licheniformis turn out to be the most potent bioagent, with a maximum amount of CaCO3 precipitate of 3.14g/100mL in the optimal conditions.
World Academy of Science, Engineering and Technology, International Journal of Biotechnology and Bioengineering, May 16, 2016
Journal on Processing and Energy in Agriculture, 2019
In this study, the influence of cultivation time on xanthan biosynthesis by usingXanthomonascampe... more In this study, the influence of cultivation time on xanthan biosynthesis by usingXanthomonascampestris ATCC 13951 on effluents from different stages of white wine production was examined in order to determine the critical bioprocess parameters. The obtained results show that after 96h of cultivation there is no significant change in the concentration of sugar components in all investigated media, and consequently there is no significant production of biopolymer, which indicates that the reduction of cultivation time for 24 h is possible without negative effect on the yield of desired product. It has also been found that the consumption of total nitrogen was noticeably higher in relation to the amount of ammonia and amino compounds, indicating that the wastewaters generated during the white wine production contain other nitrogen components that the applied producing strain can metabolize.
Bioethanol, biodiesel and biogas represent very significant alternatives to fossil fuels in order... more Bioethanol, biodiesel and biogas represent very significant alternatives to fossil fuels in order to reduce carbondioxide emissions, so there is a need to develop energy efficient technologies, equipment and waste management procedures to make production profitable by reducing production costs. One way to accomplish this is to solve the problem of waste effluents by using them to obtain high-value products. All biotechnological processes are energy intensive to some extent, so saving energy becomes one of the main goals of all biotechnological processes in addition to preserving the environment and the management or reuse of waste. The availability of waste effluents from the production of biofuels, such as distillery stillage and crude glycerol, will increase over the next few years due to the growth of the biofuel industry and will provide a great possibility for the biotechnological production of high-value products.
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Papers by Jovana Grahovac