Papers by José Buratini Junior
Journal of Assisted Reproduction and Genetics
Journal of Assisted Reproduction and Genetics
Zygote, 2021
Given the importance of embryo developmental competence assessment in reproductive medicine and b... more Given the importance of embryo developmental competence assessment in reproductive medicine and biology, the aim of this study was to compare the performance of fertilization and cleavage morphokinetics with embryo morphology to predict post-ICSI live birth. Data from embryos cultured in a time-lapse microscopy (TLM) incubator and with known live birth outcomes (LB: embryos achieving live birth, n = 168; NLB: embryos not achieving live birth, n = 1633) were used to generate receiver operating characteristic (ROC) curves based on morphokinetic or morphological scores, and the respective areas under the curve (AUC) were compared. The association between live birth and 12 combinations of four morphokinetic quality degrees (A–D) with three morphological quality degrees (A–C) was assessed using multivariate analysis. Morphokinetic parameters from tPNa to t8 were reached earlier in LB compared with NLB embryos. The ROC curve analysis indicated that morphokinetic information is more accura...
Arq Neuropsiquiatr, Sep 1, 1999
Reproduction, Fertility and Development, 2010
Embryo transfer is a biotechnology that has been used worldwide to increase the production of off... more Embryo transfer is a biotechnology that has been used worldwide to increase the production of offspring from female bovines. Treatments to induce multiple ovulations (superovulation) have evolved from superstimulatory protocols that depended upon detection of oestrus to treatments that synchronise follicle growth and ovulation, allowing for improved donor management and fixed-timed AI (FTAI). The protocols associated with FTAI facilitate animal handling and produce at least as many viably embryos as conventional treatment protocols that required detection of oestrus. Recent knowledge regarding LH receptors (LHR) and follicular development can be applied to improve embryo transfer protocols. In fact, improvements in the superstimulatory treatment called the ‘P-36 protocol’, which include hormones that stimulate LHR, indicate that adjustments related to LHR availability may increase bovine embryo yield compared with conventional protocols based on the detection of oestrus.
Neurosurgical Focus, 2002
Object The authors conducted a study to assess the efficacy of surgery in patients who underwent ... more Object The authors conducted a study to assess the efficacy of surgery in patients who underwent magnetic resonance (MR) imaging alone for localization of foci in temporal lobe epilepsy (TLE). Methods One hundred patients (43 men, 57 women) with a clinical diagnosis of TLE were prospectively studied (mean age 28 ± 9 years [± standard deviation {SD}]). All patients underwent high-resolution MR imaging, and in all unilateral mesial temporal sclerosis (MTS) was diagnosed by visual inspection. All patients underwent interictal pre-operative electroencephalography (EEG) and in 87 patients pre- and 1-year postoperative neuropsychological testing was performed. Both EEG and neuropsychological examinations were conducted in a blinded fashion, and these data were not taken into account during the surgery-related decision-making process. All patients underwent a corti-coamygdalohippocampectomy at the side of the MTS. Surgery-related outcome was rated as Class I (seizure free or simple partial...
Journal of Pediatric Endocrinology and Metabolism, 2002
Two boys are described with precocious puberty (PP) due to pineal immature teratoma associated wi... more Two boys are described with precocious puberty (PP) due to pineal immature teratoma associated with choriocarcinoma. Patient 1 was a 7 year-old boy with a 2-year history of PP. He had elevated CSF and plasma ß-hCG levels. Magnetic resonance imaging (MRI) showed a 3.0 cm pineal mass. He was initially submitted to a trial with radiotherapy, followed by radical surgical resection, stereotactic radiotherapy and chemotherapy. Long-term follow up included the appearance of acute hydrocephalus requiring CSF shunting, local hemorrhage and extensive radionecrosis. Death occurred 1.5 years after diagnosis. Patient 2 was a 7 year-old boy with an 8-month history of PP. He had elevated CSF and plasma ß-hCG and afetoprotein levels. MRI showed a 1.0 cm pineal mass. He was submitted to radical surgical resection (which caused normalization of levels of markers) and prophylactic chemotherapy. The boy is doing well 1.5 years after diagnosis. An extensive review of the literature corroborates the idea that this last treatment paradigm (surgery and chemotherapy) probably represents the best treatment regimen for these patients.
Epilepsia, 2001
To study the efficacy of extensive coverage of the brain surface with subdural grids in defining ... more To study the efficacy of extensive coverage of the brain surface with subdural grids in defining extratemporal cortical areas amenable for resection in patients with refractory extratemporal epilepy (R-ExTE) and normal or nonlocalizing magnetic resonance imaging (MRI) scans. Methods: Sixteen patients with R-ExTE were studied. Eleven patients had simple partial, eight had complex partial, and three had supplementary motor area seizures. Seizure frequency ranged from three per month to daily episodes. Interictal EEG showed large focal spiking areas in 11 patients, secondary bilateral synchrony in four, and was normal in one patient. Surface ictal recordings were nonlocalizing in six patients, and in 10, they disclosed large ictal focal spiking areas. MRI was normal in 10 patients, and in six patients, focal nonlocalizing potentially epileptogenic lesions were found. All patients were given an extensive coverage of the cortical convexity with subdural electrodes through large unilateral (n ס 13) or bilateral (n ס 3) craniotomies. Bipolar cortical stimulation was carried out through the implanted electrodes. Results: Interictal invasive recording findings showed widespread spiking areas in 13 patients and secondary bilateral synchrony in three. Ictal invasive recordings showed focal seizure onset in all patients. There were six frontal, two parietal, one temporooccipital, four rolandic, and three posterior quadrant resections. Thirteen patients had been rendered seizure free after surgery, and three had ജ90% of seizure-frequency reduction. Pathologic findings included gliosis (n ס 10), cortical dysplasia (n ס 5), or no abnormalities (n ס 1). Six patients had transient postoperative neurologic morbidity. Conclusions: Extensive subdural electrodes coverage seems to be an effective way to investigate patients with R-ExTE and normal or nonlocalizing MRI findings.
Arquivos de Neuro-Psiquiatria, 2001
PURPOSE: To study the seizure's outcome in patients with refractory epilepsy and normal MRI s... more PURPOSE: To study the seizure's outcome in patients with refractory epilepsy and normal MRI submitted to resections including the rolandic cortex. METHODS: Four adult patients were studied. All patients had motor or somatosensory simple partial seizures and normal MRI and were submitted to subdural grids' implantation with extensive coverage of the cortical convexity (1 in the non-dominant and 3 in the dominant hemisphere). RESULTS: ECoG was able to define focal areas of seizures' onset in every patient. All patients were submitted to resection of the face and tongue motor and sensitive cortex; two patients had resections including the perirolandic cortex and 2 had additional cortical removals. Three patients are seizures' free and one had a greater then 90% reduction in seizure frequency. CONCLUSION: Resections including the face and tongue rolandic cortex can be safely performed even within the dominant hemisphere.
Arquivos de Neuro-Psiquiatria, 1999
Epilepsia refratária ao tratamento medicamentoso é condição que interfere direta e negativamente ... more Epilepsia refratária ao tratamento medicamentoso é condição que interfere direta e negativamente na qualidade de vida dos pacientes, dificultando-lhes principalmente a integração social. O tratamento cirúrgico tem se mostrado eficaz no controle das crises em casos refratários, ocupando lugar importante no tratamento das epilepsias. Avaliamos a qualidade de vida de pacientes epilépticos, antes e após o tratamento cirúrgico, através de um questionário sobre qualidade de vida, adaptado do QOLIE-10 aplicado em 12 indivíduos epilépticos adultos, operados consecutivamente. O questionário, com 10 perguntas, envolvendo aspectos psicossociais e relacionados às drogas antiepilépticas, foi respondido no período pré-cirúrgico e repetido num intervalo médio de 8 meses após a cirurgia. Na comparação do questionário no período pré-operatório com o período pós-operatório, observamos diferenças estatisticamente significantes em 70% das perguntas, mostrando melhora da qualidade de vida após a cirurgi...
Arquivos de Neuro-Psiquiatria, 2000
PURPOSE: The introduction of new technologies in the clinical practice have greatly decreased the... more PURPOSE: The introduction of new technologies in the clinical practice have greatly decreased the number of patients submitted to invasive recordings. On the other hand, some patients with refractory temporal lobe epilepsy have normal MR scans or bilateral potentially epileptogenic lesions. This paper reports the results of invasive neurophysiology and surgical outcome in such patients. METHOD: Sixteen patients were studied. Eleven had normal MRI (Group I) and five had bilateral mesial temporal sclerosis (Group II). All patients had BITLS and non-localizatory seizures on video-EEG monitoring. All patients were implanted bilaterally with 32-contacts subdural grids. They were submitted to a cortico-amygdalo-total hippocampectomy at the side defined by chronic electrocorticography (ECoG). RESULTS: In Group I, seizures came from a single side in nine patients. In nine patients, seizures started at one side, spread to the ipsolateral contacts and contralaterally afterwards. On the other ...
Arquivos de Neuro-Psiquiatria, 2000
RATIONALE: The need for invasive monitoring in patients with refractory epilepsy has been greatly... more RATIONALE: The need for invasive monitoring in patients with refractory epilepsy has been greatly reduced by the introduction of new technologies such as PET, SPECT and MRI in the clinical practice. On the other hand, 10 to 30% of the patients with refractory epilepsy have non-localizatory non-invasive preoperative work-up results. This paper reports on the paradigms for subdural electrodes implantation in patients with different refractory epileptic syndromes. METHODS: Twenty-nine adult refractory epileptic patients were studied. Patients were divided into five different epileptic syndromes that represented the majority of the patients who needed invasive recordings: bitemporal (Group I; n=16 ), bi-frontal-mesial (Group II, n=5), hemispheric (Group III; n=2), anterior quadrant (Group IV; n=3) and posterior quadrant (Group V; n=3). All of them were submitted to extensive subdural electrodes' implantation (from 64 to 160 contacts) covering all the cortical surface potentially inv...
Arquivos de Neuro-Psiquiatria, 1999
Frontal lobe epilepsies may present difficulties in focus localization in the pre-operative work-... more Frontal lobe epilepsies may present difficulties in focus localization in the pre-operative work-up for epilepsy surgery. This is specially true in patients with normal MRIs. We report on a 16 years-old girl that started with seizures by the age of 8 years. They were brief nocturnal episodes with automatisms such as bicycling and boxing. Seizure frequency ranged from 4-10 per night. Scalp EEG showed few right frontal convexity spiking and intense secondary bilateral syncrhony (SBS). High resolution MRI directed to the frontal lobes was normal. Ictal SPECT suggested a right fronto-lateral focus. Ictal video-EEG showed no focal onset. She was submitted to invasive recordings after subdural plates implantation. Electrodes covered all the frontal convexity and mesial surface bilaterally. Ictal recordings disclosed stereotyped seizures starting from the right mesial frontal. Using a high-resolution tool to measure intra and interhemispheric latencies, the timing and direction of seizure ...
The control of placental hormone biosynthesis is critical during gestation, since their coordinat... more The control of placental hormone biosynthesis is critical during gestation, since their coordinated action is essential for the normal progress of pregnancy. Hormonal synthesis regulation in placenta is still not elucidated and differs from that observed in other steroidogenic tissues since specific tropic hormones have not yet been identified. Cellular localization of growth factors in the placenta, including VEGF, EG-VEGF and bFGF, points that these factors have additional roles in the organ besides their well known modulation on cell proliferation and angiogenesis. In vitro experiments bring new evidence that growth factors play regulatory roles modulating processes related to steroid hormone secretion in the placenta. Importance of local estrogen function has been highlighted and a key enzyme for its synthesis is aromatase cytochrome P450. The objective of this review was to describe some aspects of placental steroidogenesis, mainly focusing on aromatase cytochrome P450 steroidogenic enzyme expression and growth factors as others potential modulators of hormonal synthesis in the organ.
Reproduction, Fertility and Development, 2013
Members of the IGF family are key intra-ovarian regulators of follicle growth, selection, and atr... more Members of the IGF family are key intra-ovarian regulators of follicle growth, selection, and atresia. Growth differentiation factor 9 (GDF9) induces follicular somatic cells to undergo mitosis and differentiation during follicular development. Cattle from Bos indicus breeds are slower to reach sexual maturity and have longer calving intervals when compared with Bos taurus breeds (Luna-Nevarez et al. 2011). On the other hand, indicine cattle have greater number of ovarian follicles recruited per oestrous cycle when compared with taurine breeds (Alvarez et al. 2000). Our objective was to evaluate the expression of IGF1, IGFR1, IGF2, and GDF9 genes in follicles dissected from Nelore and Angus heifers with high (HFC) and low (LFC) follicle counts. Eighteen Nelore heifers and 22 Angus heifers (≈24 months old) were kept on Brachiaria brizantha grass and fed with a mix of grains with minerals and water ad libitum. Oestrous cycle was synchronized with 2 doses of PGF2α 11 days apart. Heifer...
Reproduction, Fertility and Development, 2013
During follicle development, androgens are synthesised by thecal cells under LH influence through... more During follicle development, androgens are synthesised by thecal cells under LH influence through the enzymatic conversion of the androgen precursors. In cattle, androgen concentration is positively correlated with the number of antral follicles. This experiment was designed to assess mRNA levels of androgen-producing enzymes (CYP11A1, CYP17A1, HSD3B, HSD17B) in Nelore and Angus heifers with high (HFC) and low follicle counts (LFC). Eighteen Nelore and 22 Angus heifers (≈24 months old) were kept on Brachiaria brizantha grass supplemented with a mix of grains. To determine the number of follicles, heifers were scanned with an ultrasound device (Mindray Vet DPS 2200, São Paulo, Brazil) with a 7.5-MHz probe 1 day after ovulation for 3 consecutive oestrous cycles. The first and third (before slaughter) oestrous cycles were synchronized with 2 doses of PGF2α 11 days apart. Animals were slaughtered ≈24 h after ovulation of the third cycle; 3 follicles from 2 to 4 mm in diameter were disse...
Reproduction, 2005
Paracrine cell signaling is believed to be important for ovarian follicle development, and a role... more Paracrine cell signaling is believed to be important for ovarian follicle development, and a role for some members of the fibroblast growth factor (FGF) family has been suggested. In the present study, we tested the hypothesis that FGF-8 and its cognate receptors (FGFR3c and FGFR4) are expressed in bovine antral follicles. RT-PCR was used to analyze bovineFgf8,Fgfr3candFgfr4mRNA levels in oocytes, and granulosa and theca cells.Fgf8expression was detected in oocytes and in granulosa and theca cells; this expression pattern differs from that reported in rodents. Granulosa and theca cells, but not oocytes, expressedFgfr3c, and expression in granulosa cells increased significantly with follicle estradiol content, a major indicator of follicle health.Fgfr4expression was restricted to theca cells in the follicle, and decreased significantly with increasing follicle size. To investigate the potential regulation ofFgfr3cexpression in the bovine granulosa, cells were cultured in serum-free m...
Reproduction, Fertility and Development, 2010
A member of the FGF7 subfamily, FGF10 acts via FGFR2B and FGFR1B. In bovine antral follicles, FGF... more A member of the FGF7 subfamily, FGF10 acts via FGFR2B and FGFR1B. In bovine antral follicles, FGF-10 was detected in oocytes and theca cells (TC). Levels of mRNA were negatively correlated with intrafollicular concentrations of estradiol, and FGF10 inhibited estradiol production from granulosa cells (GC). In Nellore (Bos indicus), morphological divergence occurs on average 2.5 days after ovulation, when dominant follicle diameter is around 6.0 mm. To gain insight into the involvement of the FGF10 system in the control of follicle selection, we assessed mRNA expression of FGF10 in TC and of FGFR1B and FGFR2B in GC from dominant and subordinate follicles around deviation in Nellore heifers. Thirteen Nellore heifers were hormonally synchronized, and ovulation was detected by ultrasound monitoring every 12 h. Heifers were slaughtered 2 (n =4), 2.5 (n = 5), and 3 (n = 4) days after ovulation. Granulosa cells and TC were separated from the 2 largest follicles and submitted to total RNA ex...
Naunyn-Schmiedeberg's Archives of Pharmacology, 2007
It has been recently shown that the supersensitivity of distal segments of the rat tail artery to... more It has been recently shown that the supersensitivity of distal segments of the rat tail artery to phenylephrine after chemical sympathectomy with reserpine results from the appearance of α 1D-adrenoceptors. It is known that both α 1A-and α 1D-adrenoceptors are involved in the contractions of proximal portions of the rat tail artery. Therefore, this study investigated whether sympathectomy with reserpine would induce supersensitivity in proximal segments of the rat tail artery, a tissue in which α 1Dadrenoceptors are already functional. Proximal segments of tail arteries from reserpinised rats were three-to sixfold more sensitive to phenylephrine and methoxamine than were arteries from control rats (n=6-2; p<0.05). The imidazolines N-[5-(4,5-Dihydro-1H-imidazol-2-yl)-2-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl]methanesulfonamide hydrobromide (A-61603) and oxymetazoline, which activate selectively α 1A-adrenoceptors, were equipotent in tail arteries from control and reserpinised rats (n=4-2; p< 0.05), whereas buspirone, which activates selectively α 1Dadrenoceptor, was ≈4-fold more potent in tail arteries from reserpinised rats (n=4-6; p<0.05). Prazosin (nonselective) and 5-methylurapidil (α 1A-selective), were competitive antagonists of contractions induced by phenylephrine and were equipotent in tail arteries from control and reserpinised rats (n=4-6). The selective α 1D-adrenoceptor antagonist 8-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-8-azaspiro [4.5]decane-7,9-dione dihydrochloride (BMY-7378) presented similar complex antagonism in tail arteries from control and reserpinised rats, with Schild slopes much lower than 1.0 (p<0.05, n=4-6). Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) revealed that mRNA encoding α 1A-and α 1B-adrenoceptors are similarly distributed in tail arteries from control and reserpinised rats, whereas mRNA for α 1D-adrenoceptors is twice more abundant in the tail artery from reserpinised rats. In conclusion, the supersensitivity induced by reserpine is related only to α 1D-adrenoceptors, even in tissues where this receptor subtype is already present and functional. Only the use of subtype-selective α 1-adrenoceptor agonists detected the increased α 1D-adrenoceptor component after reserpinisation, as the antagonists behaved similarly in tail arteries from control and reserpinised rats.
Journal of Pharmacology and Experimental Therapeutics, 2005
The rat tail artery has been used for the study of vasoconstriction mediated by ␣ 1A-adrenoceptor... more The rat tail artery has been used for the study of vasoconstriction mediated by ␣ 1A-adrenoceptors (ARs). However, rings from proximal segments of the tail artery (within the initial 4 cm, PRTA) were at least 3-fold more sensitive to methoxamine and phenylephrine (n ϭ 6-12; p Ͻ 0.05) than rings from distal parts (between the sixth and 10th cm, DRTA). Interestingly, the imidazolines N-[5-(4,5-dihydro-1H-imidazol-2-yl)-2-hydroxy-5,6,7,8-tetrahydronaphthalen-1-yl]methanesulfonamide hydrobromide (A-61603) and oxymetazoline, which activate selectively ␣ 1A-ARs, were equipotent in PRTA and DRTA (n ϭ 4-12), whereas buspirone, which activates selectively ␣ 1D-AR, was Ϸ70-fold more potent in PRTA than in DRTA (n ϭ 8; p Ͻ 0.05). The selective ␣ 1D-AR antagonist 8-[2-[4-(methoxyphenyl)-1piperazinyl]ethyl]-8-azaspiro[4.5]decane-7,9-dione dihydrochloride (BMY-7378) was Ϸ70-fold more potent against the contractions induced by phenylephrine in PRTA (pK B of Ϸ8.45; n ϭ 6) than in DRTA (pK B of Ϸ6.58; n ϭ 6), although the antagonism was complex in PRTA. 5-Methylurapidil, a selective ␣ 1A-antagonist, was equipotent in PRTA and DRTA (pK B of Ϸ8.4), but the Schild slope in DRTA was 0.73 Ϯ 0.05 (n ϭ 5). The noncompetitive ␣ 1B-antagonist conotoxin-TIA reduced the maximal contraction induced by phenylephrine in DRTA, but not in PRTA. These results indicate a predominant role for ␣ 1A-ARs in the contractions of both PRTA and DRTA but with significant coparticipations of ␣ 1D-ARs in PRTA and ␣ 1B-ARs in DRTA. Semiquantitative reverse transcription-polymerase chain reaction revealed that mRNA encoding ␣ 1A-and ␣ 1B-ARs are similarly distributed in PRTA and DRTA, whereas mRNA for ␣ 1D-ARs is twice more abundant in PRTA. Therefore, ␣ 1-ARs subtypes are differentially distributed along the tail artery. It is important to consider the segment from which the tissue preparation is taken to avoid misinterpretations on receptor mechanisms and drug selectivities.
Uploads
Papers by José Buratini Junior