Cada generación está llamada a enfrentar su papel protagónico que le marca su tiempo y su espacio... more Cada generación está llamada a enfrentar su papel protagónico que le marca su tiempo y su espacio. Los integrantes de cada generación, es decir, del grupo de personas que han vivido ciertas etapas de sus vidas con similitud de experiencias, con entornos culturales y sociales semejantes, se sienten identificados, atraídos a actuar en común, a defender con la misma fuerza, el mismo ideal.
Normal semilunar valves develop at the downstream end of the embryonic cardiac tube by remodeling... more Normal semilunar valves develop at the downstream end of the embryonic cardiac tube by remodeling of the endocardial cushion material. Two valves, each with three commissures, form by subdivision of the two larger (lateral) of the four endocardial cushions. In dfis study congenitally malformed semihmar valves from autopsy heart specimens were reviewed to determine whether the lesions could be explained as deviations of normal valvulogenesis. The morphological spectrum of raphes and their occasional occurrence at the site of separation between die two lateral cushions, an obligatory conmfissure, suggested that they are usually dm result of fusion of previously formed commissures. In 306 hearts there were 316 nmlformed semihmar valves: 208 puhnonic and 108 aortic. Four categories of lesions were found: (1) 27 atretic valves could not be interpreted; (2) 6 valves were quadricuspid with an extra commissure; (3) 45 valves showed failure of formation of one (44) or two (1) commissures; and (4)238 valves showed fusion of one (156), two (22), or three (60) commissures as indicated by the presence of raphes. In 27 instances an atretic valve was too small to evaluate. Thus, evidence that three leaflets had at one time been present, as determined by the count of commissures plus raphes, was found in 82 per cent of interpretable valves. Several associated congenital cardiac lesions (coarctation of the aorta, transposition of the great vessels, tetralogy of Fallot, mitral atresia) exhibited a highly significant association with either aortic or pulmonic valvular malformations. The findings suggest that the majority of congenital malformations of the semilunar valves are due to lesions acquired in utero subsequent to normal valvulogenesis. Disproportionate flow reduction of the right or left side of the heart may be a factor in the development of malformations in the respective semilunar valve.
Biochemical and Biophysical Research Communications, 2003
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurific... more This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200 kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmo... more Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmonary atresia at the age of 1 month. Then he underwent right and left Blalock-Taussig shunts at the ages of 2 months and 5 years, respectively. His cyanosis had increased at 20 years of age. Cardiac catheterization showed occlusion of the left Blalock-Taussig shunt and absence of the left pulmonary artery. Lung perfusion scintigram showed late phase perfusion in the left lung. Chest computed tomographic scan demonstrated the left pulmonary artery. We describe the operative technique of total correction.
With the object of analyzing current characteristics of infectious endocarditis (IE) in children,... more With the object of analyzing current characteristics of infectious endocarditis (IE) in children, we carried out a retrospective study of 23 cases of IE in children under 15 years of age seen at the Hospital Ramón y Cajal in Madrid (Spain) between 1977 and 1985. The incidence was high (1.3 cases per 1000 children admitted). The male/female ratio was 2∶1. Eight patients were under 2 years of age and 15 over 2 years, the majority being adolescents. The two groups presented marked etiological and prognostic differences. Congenital heart disease was the predisposing factor in 20 of the 23 cases.Streptococcus viridans (nine cases) andStaphylococcus aureus (eight cases) were the most frequent organisms. Fourteen cases were on a native valve and nine were secondary (seven of these on prosthetic patches). In spite of advances in therapy, IE continues to be a severe illness: the mortality rate in our series was 26%. Factors associated with a poor prognosis were: age less than 2 years,Staphylococcus aureus as the causative agent, and the presence of prosthetic material.
Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmo... more Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmonary atresia at the age of 1 month. Then he underwent right and left Blalock-Taussig shunts at the ages of 2 months and 5 years, respectively. His cyanosis had increased at 20 years of age. Cardiac catheterization showed occlusion of the left Blalock-Taussig shunt and absence of the left pulmonary artery. Lung perfusion scintigram showed late phase perfusion in the left lung. Chest computed tomographic scan demonstrated the left pulmonary artery. We describe the operative technique of total correction.
With the object of analyzing current characteristics of infectious endocarditis (IE) in children,... more With the object of analyzing current characteristics of infectious endocarditis (IE) in children, we carried out a retrospective study of 23 cases of IE in children under 15 years of age seen at the Hospital Ramón y Cajal in Madrid (Spain) between 1977 and 1985. The incidence was high (1.3 cases per 1000 children admitted). The male/female ratio was 2∶1. Eight patients were under 2 years of age and 15 over 2 years, the majority being adolescents. The two groups presented marked etiological and prognostic differences. Congenital heart disease was the predisposing factor in 20 of the 23 cases.Streptococcus viridans (nine cases) andStaphylococcus aureus (eight cases) were the most frequent organisms. Fourteen cases were on a native valve and nine were secondary (seven of these on prosthetic patches). In spite of advances in therapy, IE continues to be a severe illness: the mortality rate in our series was 26%. Factors associated with a poor prognosis were: age less than 2 years,Staphylococcus aureus as the causative agent, and the presence of prosthetic material.
Cada generación está llamada a enfrentar su papel protagónico que le marca su tiempo y su espacio... more Cada generación está llamada a enfrentar su papel protagónico que le marca su tiempo y su espacio. Los integrantes de cada generación, es decir, del grupo de personas que han vivido ciertas etapas de sus vidas con similitud de experiencias, con entornos culturales y sociales semejantes, se sienten identificados, atraídos a actuar en común, a defender con la misma fuerza, el mismo ideal.
Normal semilunar valves develop at the downstream end of the embryonic cardiac tube by remodeling... more Normal semilunar valves develop at the downstream end of the embryonic cardiac tube by remodeling of the endocardial cushion material. Two valves, each with three commissures, form by subdivision of the two larger (lateral) of the four endocardial cushions. In dfis study congenitally malformed semihmar valves from autopsy heart specimens were reviewed to determine whether the lesions could be explained as deviations of normal valvulogenesis. The morphological spectrum of raphes and their occasional occurrence at the site of separation between die two lateral cushions, an obligatory conmfissure, suggested that they are usually dm result of fusion of previously formed commissures. In 306 hearts there were 316 nmlformed semihmar valves: 208 puhnonic and 108 aortic. Four categories of lesions were found: (1) 27 atretic valves could not be interpreted; (2) 6 valves were quadricuspid with an extra commissure; (3) 45 valves showed failure of formation of one (44) or two (1) commissures; and (4)238 valves showed fusion of one (156), two (22), or three (60) commissures as indicated by the presence of raphes. In 27 instances an atretic valve was too small to evaluate. Thus, evidence that three leaflets had at one time been present, as determined by the count of commissures plus raphes, was found in 82 per cent of interpretable valves. Several associated congenital cardiac lesions (coarctation of the aorta, transposition of the great vessels, tetralogy of Fallot, mitral atresia) exhibited a highly significant association with either aortic or pulmonic valvular malformations. The findings suggest that the majority of congenital malformations of the semilunar valves are due to lesions acquired in utero subsequent to normal valvulogenesis. Disproportionate flow reduction of the right or left side of the heart may be a factor in the development of malformations in the respective semilunar valve.
Biochemical and Biophysical Research Communications, 2003
This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurific... more This paper provides an evaluation of a plant-derived HBsAg-specific antibody in the immunopurification of the recombinant HBsAg for vaccine purposes. This plant-derived antibody was obtained from different batches of 100-200 kg of tobacco leaves and coupled to Sepharose CL-4B with high efficiency. The plant-derived antibody immunoaffinity matrix purification behavior (elution capacity, antigen purity, purification cycles, and ligand leakage) was comparable to that of its mouse-derived monoclonal antibody homolog. This result supports the feasibility of using this plant-derived antibody for the immunopurification of the Hepatitis B surface antigen for human use, opening a new possibility to overcome the constrain of monoclonal antibody production in mice.
Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmo... more Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmonary atresia at the age of 1 month. Then he underwent right and left Blalock-Taussig shunts at the ages of 2 months and 5 years, respectively. His cyanosis had increased at 20 years of age. Cardiac catheterization showed occlusion of the left Blalock-Taussig shunt and absence of the left pulmonary artery. Lung perfusion scintigram showed late phase perfusion in the left lung. Chest computed tomographic scan demonstrated the left pulmonary artery. We describe the operative technique of total correction.
With the object of analyzing current characteristics of infectious endocarditis (IE) in children,... more With the object of analyzing current characteristics of infectious endocarditis (IE) in children, we carried out a retrospective study of 23 cases of IE in children under 15 years of age seen at the Hospital Ramón y Cajal in Madrid (Spain) between 1977 and 1985. The incidence was high (1.3 cases per 1000 children admitted). The male/female ratio was 2∶1. Eight patients were under 2 years of age and 15 over 2 years, the majority being adolescents. The two groups presented marked etiological and prognostic differences. Congenital heart disease was the predisposing factor in 20 of the 23 cases.Streptococcus viridans (nine cases) andStaphylococcus aureus (eight cases) were the most frequent organisms. Fourteen cases were on a native valve and nine were secondary (seven of these on prosthetic patches). In spite of advances in therapy, IE continues to be a severe illness: the mortality rate in our series was 26%. Factors associated with a poor prognosis were: age less than 2 years,Staphylococcus aureus as the causative agent, and the presence of prosthetic material.
Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmo... more Our patient was diagnosed with complete atrioventricular canal and Tetralogy of Fallot with pulmonary atresia at the age of 1 month. Then he underwent right and left Blalock-Taussig shunts at the ages of 2 months and 5 years, respectively. His cyanosis had increased at 20 years of age. Cardiac catheterization showed occlusion of the left Blalock-Taussig shunt and absence of the left pulmonary artery. Lung perfusion scintigram showed late phase perfusion in the left lung. Chest computed tomographic scan demonstrated the left pulmonary artery. We describe the operative technique of total correction.
With the object of analyzing current characteristics of infectious endocarditis (IE) in children,... more With the object of analyzing current characteristics of infectious endocarditis (IE) in children, we carried out a retrospective study of 23 cases of IE in children under 15 years of age seen at the Hospital Ramón y Cajal in Madrid (Spain) between 1977 and 1985. The incidence was high (1.3 cases per 1000 children admitted). The male/female ratio was 2∶1. Eight patients were under 2 years of age and 15 over 2 years, the majority being adolescents. The two groups presented marked etiological and prognostic differences. Congenital heart disease was the predisposing factor in 20 of the 23 cases.Streptococcus viridans (nine cases) andStaphylococcus aureus (eight cases) were the most frequent organisms. Fourteen cases were on a native valve and nine were secondary (seven of these on prosthetic patches). In spite of advances in therapy, IE continues to be a severe illness: the mortality rate in our series was 26%. Factors associated with a poor prognosis were: age less than 2 years,Staphylococcus aureus as the causative agent, and the presence of prosthetic material.
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Papers by José Brito