Annals of Clinical Microbiology and Antimicrobials, 2020
Background Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is an emerging viral pand... more Background Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is an emerging viral pandemic disease. In the last 6 months, SARS-CoV-2 has caused millions of reported cases and hundreds of thousands of deaths. As other world regions, South America has not contained the pandemic’s advance since it lacks the hospital and economic capacities. Public health implications of transmission, while the asymptomatic/presymptomatic infection is a critical concern at the current pandemic. Objective Describe the socio-demographic, clinical, and viral features of a cohort of SARS-CoV-2 infected individuals from the Colombian Caribbean. Methods Six hundred eighty-six clinical samples of suspected SARS-CoV-2 infection cases and contacts individuals from several hospital centers in the department of Córdoba, Colombia, were received at our laboratory between April 9th and May 16th, 2020. RNA was extracted using lysis buffers and spin columns. The samples were tested for SARS-CoV-2 by reverse ...
Barrera et al Conclusiones: Los hallazgos demuestran exposición previa a Rickettsia sp. y a Hanta... more Barrera et al Conclusiones: Los hallazgos demuestran exposición previa a Rickettsia sp. y a Hantavirus en la población indígena de Tuchín. Los resultados pueden ser útiles para establecer una alerta sobre estas fiebres hemorrágicas. Aunque no se hallaron seropositivos para Chikungunya, este fue el primer trabajo de vigilancia epidemiológica realizado en Colombia sobre este virus.
Biomédica : revista del Instituto Nacional de Salud, 2011
Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rod... more Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rodents are usually parasitized by the tick vector, Amblyomma cajennense, the main vector of rickettsioses in humans and animals in South America. Capybaras can be used as sentinels in detection of circulation of rickettsiae. Antibodies to rickettsiae of spotted fever group were detected in capybaras in a rural area of Cordoba Province, northern Colombia. Sera were analyzed from 36 capybaras in a rural area of Monteria (village of San Jeronimo) in Córdoba. For the detection of IgG antibodies, indirect immunofluorescence was performed. The antigens were derived from R. rickettsia strain Taiaçu isolated in Brazil. Capybara sera were diluted 1:64 for IFA analysis. Ticks were collected from each capybara (also known as chigüiro) and identified to species. The seroprevalence of spotted fever group Rickettsia was 22% (8 capybaras). Four sera had a titer of 1:64, 3 had a titer of 1:128 and one ser...
The purpose of this study was to provide molecular evidence of Rickettsia spp. in ticks collected... more The purpose of this study was to provide molecular evidence of Rickettsia spp. in ticks collected from 2 sites of Cordoba. From May to June 2009, 1069 Amblyomma cajennense ticks were removed from 40 capybaras (Hydrochoerus hydrochaeris) in a rural locality of Monteria. Furthermore, 458 Amblyomma sp. larvae and 20 Amblyomma sp. nymphs were collected in a rural locality of Los Cordobas (Cordoba) by drag sampling on vegetation (n=1547). Ticks were grouped into pools and tested for rickettsial infection by real-time PCR targeting the rickettsial gene gltA. Subsequently, PCR targeting for gltA, ompA, ompB, and 16S rRNA, sequencing, and phylogenetic analyses were undertaken. Rickettsial DNA was detected in 10 (4.6%) out of 214 pools of ticks by RT-PCR. Five (33%) of free-living Amblyomma sp. larval pools were positive, as well as 5 (2.6%) pools from A. cajennense. Only the gltA gene was amplified from 5 pools of free-living larvae. The nucleotide sequences were 100% identical to R. bellii by BLAST. Only one pool from A. cajennense was positive for gltA, ompA, ompB, and 16S rRNA. The partial nucleotide sequences of these genes were 100% identical to nucleotide sequences of the same genes of a new proposed species Candidatus Rickettsia sp. strain Colombianensi. This is the first report of R. bellii in ticks in Colombia and the second report of detection of Candidatus Rickettsia sp. strain Colombianensi. These Rickettsia species are still considered of unknown pathogenicity. Further studies are needed to characterize the ecological and potential pathogenic role of these 2 Rickettsia species found in Cordoba.
Annals of Clinical Microbiology and Antimicrobials, 2020
Background Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is an emerging viral pand... more Background Severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) is an emerging viral pandemic disease. In the last 6 months, SARS-CoV-2 has caused millions of reported cases and hundreds of thousands of deaths. As other world regions, South America has not contained the pandemic’s advance since it lacks the hospital and economic capacities. Public health implications of transmission, while the asymptomatic/presymptomatic infection is a critical concern at the current pandemic. Objective Describe the socio-demographic, clinical, and viral features of a cohort of SARS-CoV-2 infected individuals from the Colombian Caribbean. Methods Six hundred eighty-six clinical samples of suspected SARS-CoV-2 infection cases and contacts individuals from several hospital centers in the department of Córdoba, Colombia, were received at our laboratory between April 9th and May 16th, 2020. RNA was extracted using lysis buffers and spin columns. The samples were tested for SARS-CoV-2 by reverse ...
Barrera et al Conclusiones: Los hallazgos demuestran exposición previa a Rickettsia sp. y a Hanta... more Barrera et al Conclusiones: Los hallazgos demuestran exposición previa a Rickettsia sp. y a Hantavirus en la población indígena de Tuchín. Los resultados pueden ser útiles para establecer una alerta sobre estas fiebres hemorrágicas. Aunque no se hallaron seropositivos para Chikungunya, este fue el primer trabajo de vigilancia epidemiológica realizado en Colombia sobre este virus.
Biomédica : revista del Instituto Nacional de Salud, 2011
Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rod... more Capybaras (Hydrochoerus hydrochaeris) are considered amplifying hosts of Rickettsia sp. These rodents are usually parasitized by the tick vector, Amblyomma cajennense, the main vector of rickettsioses in humans and animals in South America. Capybaras can be used as sentinels in detection of circulation of rickettsiae. Antibodies to rickettsiae of spotted fever group were detected in capybaras in a rural area of Cordoba Province, northern Colombia. Sera were analyzed from 36 capybaras in a rural area of Monteria (village of San Jeronimo) in Córdoba. For the detection of IgG antibodies, indirect immunofluorescence was performed. The antigens were derived from R. rickettsia strain Taiaçu isolated in Brazil. Capybara sera were diluted 1:64 for IFA analysis. Ticks were collected from each capybara (also known as chigüiro) and identified to species. The seroprevalence of spotted fever group Rickettsia was 22% (8 capybaras). Four sera had a titer of 1:64, 3 had a titer of 1:128 and one ser...
The purpose of this study was to provide molecular evidence of Rickettsia spp. in ticks collected... more The purpose of this study was to provide molecular evidence of Rickettsia spp. in ticks collected from 2 sites of Cordoba. From May to June 2009, 1069 Amblyomma cajennense ticks were removed from 40 capybaras (Hydrochoerus hydrochaeris) in a rural locality of Monteria. Furthermore, 458 Amblyomma sp. larvae and 20 Amblyomma sp. nymphs were collected in a rural locality of Los Cordobas (Cordoba) by drag sampling on vegetation (n=1547). Ticks were grouped into pools and tested for rickettsial infection by real-time PCR targeting the rickettsial gene gltA. Subsequently, PCR targeting for gltA, ompA, ompB, and 16S rRNA, sequencing, and phylogenetic analyses were undertaken. Rickettsial DNA was detected in 10 (4.6%) out of 214 pools of ticks by RT-PCR. Five (33%) of free-living Amblyomma sp. larval pools were positive, as well as 5 (2.6%) pools from A. cajennense. Only the gltA gene was amplified from 5 pools of free-living larvae. The nucleotide sequences were 100% identical to R. bellii by BLAST. Only one pool from A. cajennense was positive for gltA, ompA, ompB, and 16S rRNA. The partial nucleotide sequences of these genes were 100% identical to nucleotide sequences of the same genes of a new proposed species Candidatus Rickettsia sp. strain Colombianensi. This is the first report of R. bellii in ticks in Colombia and the second report of detection of Candidatus Rickettsia sp. strain Colombianensi. These Rickettsia species are still considered of unknown pathogenicity. Further studies are needed to characterize the ecological and potential pathogenic role of these 2 Rickettsia species found in Cordoba.
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Papers by Jorge Miranda