The present study describes the anti-inflammatory, anti-microbial activity and lipophilic profile... more The present study describes the anti-inflammatory, anti-microbial activity and lipophilic profile with acute toxicological studies of Urtica dioica. Successive extraction of the leaves with organic solvents of increasing polarity and their screening for anti-inflammatory and anti-microbial activity was assessed. Hexane extract showed good anti-inflammatory and anti-microbial activity; hence it was further fractionated using open silica gel column chromatography into 19 sub fractions which were pooled together according to their thin layer chromatography (TLC) profile to give an overall 5 fractions. Among the 5 fractions, fraction-II (FII) at a dose of 200 mg/kg bodyweight (bw) exhibits equipotent antiinflammatory activity (48.83% after 3 h) as that of the standard drug indomethacin (53.48%) in Wistar rats. FII also showed a potent anti-microbial activity against all the tested bacterial strains and its minimum inhibitory concentration (MIC) value was 125, 15.62, 31.25, 250, 31.25, 125 and 7.81 µg/ml against Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Shigella flexneri and Salmonella typhi, respectively which was determined by serial tube dilution method. FII was subjected to gas chromatography-mass spectrometry (GC-MS) analysis in search of potent anti-inflammatory and anti-microbial compound(s). 2,4-Di-t-butylphenol (4.56%), neophytadiene (26.97%), butyl tetradecyl ester (9.53%), dibutyl phthalate (7.45%), bis(2-ethyl hexyl) maleate (8.80%), 1,2-benzenedicarboxylic acid (9.89%) and 2-tert-butyl-4,6-bis(3,5-di-tert-butyl-4hydroxybenzyl)phenol (3.19%) were the major constituents responsible for both anti-inflammatory and anti-microbial activity of hexane extract of U. dioica. Sub-acute oral toxicity of crude n-hexane extract of U. dioica was carried out in Wistar rats at doses of 250, 500, 1000 and 2000 mg/kg bw to assess the safety index. Hematological parameters from blood and other biochemical parameters from serum confirmed its safety at tested concentrations. Our results corroborate the anti-inflammatory and antimicrobial activity of U. dioica, and could justify its use in folk medicine for the treatment of rheumatic arthritis and other infectious diseases.
Nanoremediation is an innovative branch of nanotechnology that employed promising sustainable rem... more Nanoremediation is an innovative branch of nanotechnology that employed promising sustainable remediation strategies to control pollution from contaminated sites. Bionanoremediation is an important way of remediation in which nanoparticles are biologically synthesized using plant parts or microbes. This is an effective solution to mitigate the challenge of environmental pollution. Diverse kinds of heavy metal substances and chemicals associated with agriculture can be removed from contaminated locations using nanoremediation. Various technologies have been used in environmental remediation such as adsorption, absorption, chemical reactions, photocatalysis and filtration for the removal of contaminants from the environment. The big surface area to volume ratio of nanoparticles leads to enhance reactivity. Nano-materials are also involved in cleanup of various pollutants from diverse environmental locations such as air, water and earth. The combination of nanoremediation with other bi...
Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different ... more Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different types of secondary metabolites synthesized by plants which are play important role in biotechnological application and also in antimicrobial activity. In the present study were carried out to determine in vitro the antifungal activity of alcoholic, hydroalcoholic and aqueous crude extracts of Polyalthia longifolia leaf against Rhizoctonia solani fungus. All these extracts i.e., 100% aqueous, 100% alcohol and 50% hydro alcohol used as 10mg/ml concentration with acetone solvent to control of Rhizoctonia solani. The results revealed that the extract that most effectively inhibited growth was found to be 100% alcoholic leaf extract. Finding in this study confirmed that plant extracts can be used as to develop the plant extract-based bio-formulation to control plant pathogens to reduce the dependence on the synthetic fungicides which are ecological safe and cheap.
In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Le... more In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Leaf extract has been assayed against Alternaria alternata which is responsible for leaf spot disease of chilli. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 50% alcohol extract and in hot extraction highest percent extractive value was obtained with Methanol extract. In antifungal activity 100% alcohol crude extract and partial purified acetone extract was show Maximum inhibition of Alternaria alternata. Mancozeb and water were used as standards. Results suggest that Lawsonia inermis. Leaf extract can be used to develop a bio control agent against Alternaria alternata. Lawsonia inermis plant can be used to discover bioactive natural p...
Encyclopedia of Animal Cognition and Behavior, 2020
Photoperiodism is the ability of plants and animals to measure and respond to duration of light a... more Photoperiodism is the ability of plants and animals to measure and respond to duration of light and dark in a day, or length of day and night cycle in a day, season, or year, i.e., photoperiod.
Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different ... more Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different types of secondary metabolites synthesized by plants which are play important role in biotechnological application and also in antimicrobial activity. In the present study were carried out to determine in vitro the antifungal activity of alcoholic, hydroalcoholic and aqueous crude extracts of Polyalthia longifolia leaf against Rhizoctonia solani fungus. All these extracts i.e., 100% aqueous, 100% alcohol and 50% hydro alcohol used as 10mg/ml concentration with acetone solvent to control of Rhizoctonia solani. The results revealed that the extract that most effectively inhibited growth was found to be 100% alcoholic leaf extract. Finding in this study confirmed that plant extracts can be used as to develop the plant extract-based bio-formulation to control plant pathogens to reduce the dependence on the synthetic fungicides which are ecological safe and cheap.
In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Le... more In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Leaf extract has been assayed against Alternaria alternata which is responsible for leaf spot disease of chilli. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 50% alcohol extract and in hot extraction highest percent extractive value was obtained with Methanol extract. In antifungal activity 100% alcohol crude extract and partial purified acetone extract was show Maximum inhibition of Alternaria alternata. Mancozeb and water were used as standards. Results suggest that Lawsonia inermis. Leaf extract can be used to develop a bio control agent against Alternaria alternata. Lawsonia inermis plant can be used to discover bioactive natural p...
The present study describes the anti-inflammatory, anti-microbial activity and lipophilic profile... more The present study describes the anti-inflammatory, anti-microbial activity and lipophilic profile with acute toxicological studies of Urtica dioica. Successive extraction of the leaves with organic solvents of increasing polarity and their screening for anti-inflammatory and anti-microbial activity was assessed. Hexane extract showed good anti-inflammatory and anti-microbial activity; hence it was further fractionated using open silica gel column chromatography into 19 sub fractions which were pooled together according to their thin layer chromatography (TLC) profile to give an overall 5 fractions. Among the 5 fractions, fraction-II (FII) at a dose of 200 mg/kg bodyweight (bw) exhibits equipotent antiinflammatory activity (48.83% after 3 h) as that of the standard drug indomethacin (53.48%) in Wistar rats. FII also showed a potent anti-microbial activity against all the tested bacterial strains and its minimum inhibitory concentration (MIC) value was 125, 15.62, 31.25, 250, 31.25, 125 and 7.81 µg/ml against Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Shigella flexneri and Salmonella typhi, respectively which was determined by serial tube dilution method. FII was subjected to gas chromatography-mass spectrometry (GC-MS) analysis in search of potent anti-inflammatory and anti-microbial compound(s). 2,4-Di-t-butylphenol (4.56%), neophytadiene (26.97%), butyl tetradecyl ester (9.53%), dibutyl phthalate (7.45%), bis(2-ethyl hexyl) maleate (8.80%), 1,2-benzenedicarboxylic acid (9.89%) and 2-tert-butyl-4,6-bis(3,5-di-tert-butyl-4hydroxybenzyl)phenol (3.19%) were the major constituents responsible for both anti-inflammatory and anti-microbial activity of hexane extract of U. dioica. Sub-acute oral toxicity of crude n-hexane extract of U. dioica was carried out in Wistar rats at doses of 250, 500, 1000 and 2000 mg/kg bw to assess the safety index. Hematological parameters from blood and other biochemical parameters from serum confirmed its safety at tested concentrations. Our results corroborate the anti-inflammatory and antimicrobial activity of U. dioica, and could justify its use in folk medicine for the treatment of rheumatic arthritis and other infectious diseases.
Nanoremediation is an innovative branch of nanotechnology that employed promising sustainable rem... more Nanoremediation is an innovative branch of nanotechnology that employed promising sustainable remediation strategies to control pollution from contaminated sites. Bionanoremediation is an important way of remediation in which nanoparticles are biologically synthesized using plant parts or microbes. This is an effective solution to mitigate the challenge of environmental pollution. Diverse kinds of heavy metal substances and chemicals associated with agriculture can be removed from contaminated locations using nanoremediation. Various technologies have been used in environmental remediation such as adsorption, absorption, chemical reactions, photocatalysis and filtration for the removal of contaminants from the environment. The big surface area to volume ratio of nanoparticles leads to enhance reactivity. Nano-materials are also involved in cleanup of various pollutants from diverse environmental locations such as air, water and earth. The combination of nanoremediation with other bi...
Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different ... more Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different types of secondary metabolites synthesized by plants which are play important role in biotechnological application and also in antimicrobial activity. In the present study were carried out to determine in vitro the antifungal activity of alcoholic, hydroalcoholic and aqueous crude extracts of Polyalthia longifolia leaf against Rhizoctonia solani fungus. All these extracts i.e., 100% aqueous, 100% alcohol and 50% hydro alcohol used as 10mg/ml concentration with acetone solvent to control of Rhizoctonia solani. The results revealed that the extract that most effectively inhibited growth was found to be 100% alcoholic leaf extract. Finding in this study confirmed that plant extracts can be used as to develop the plant extract-based bio-formulation to control plant pathogens to reduce the dependence on the synthetic fungicides which are ecological safe and cheap.
In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Le... more In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Leaf extract has been assayed against Alternaria alternata which is responsible for leaf spot disease of chilli. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 50% alcohol extract and in hot extraction highest percent extractive value was obtained with Methanol extract. In antifungal activity 100% alcohol crude extract and partial purified acetone extract was show Maximum inhibition of Alternaria alternata. Mancozeb and water were used as standards. Results suggest that Lawsonia inermis. Leaf extract can be used to develop a bio control agent against Alternaria alternata. Lawsonia inermis plant can be used to discover bioactive natural p...
Encyclopedia of Animal Cognition and Behavior, 2020
Photoperiodism is the ability of plants and animals to measure and respond to duration of light a... more Photoperiodism is the ability of plants and animals to measure and respond to duration of light and dark in a day, or length of day and night cycle in a day, season, or year, i.e., photoperiod.
Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different ... more Phytochemicals are chemical compounds which are also called as a secondary metabolite. Different types of secondary metabolites synthesized by plants which are play important role in biotechnological application and also in antimicrobial activity. In the present study were carried out to determine in vitro the antifungal activity of alcoholic, hydroalcoholic and aqueous crude extracts of Polyalthia longifolia leaf against Rhizoctonia solani fungus. All these extracts i.e., 100% aqueous, 100% alcohol and 50% hydro alcohol used as 10mg/ml concentration with acetone solvent to control of Rhizoctonia solani. The results revealed that the extract that most effectively inhibited growth was found to be 100% alcoholic leaf extract. Finding in this study confirmed that plant extracts can be used as to develop the plant extract-based bio-formulation to control plant pathogens to reduce the dependence on the synthetic fungicides which are ecological safe and cheap.
In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Le... more In this study antifungal activity of crude and partially purified extracts of Lawsonia inermis Leaf extract has been assayed against Alternaria alternata which is responsible for leaf spot disease of chilli. Cold and hot extraction of leaf extract was prepared in different organic solvents, which were subsequently recycled by rotary vacuum evaporator. Antifungal activity of prepared plant extracts were determined by poison food technique. In cold extraction highestpercent extractive value was obtained with 50% alcohol extract and in hot extraction highest percent extractive value was obtained with Methanol extract. In antifungal activity 100% alcohol crude extract and partial purified acetone extract was show Maximum inhibition of Alternaria alternata. Mancozeb and water were used as standards. Results suggest that Lawsonia inermis. Leaf extract can be used to develop a bio control agent against Alternaria alternata. Lawsonia inermis plant can be used to discover bioactive natural p...
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