Papers by Jadwiga Renata Ochocka
Pharmaceutics
Cyclopia sp. (honeybush) is an African shrub known as a rich source of polyphenols. The biologica... more Cyclopia sp. (honeybush) is an African shrub known as a rich source of polyphenols. The biological effects of fermented honeybush extracts were investigated. The influence of honeybush extracts on extracellular matrix (ECM) enzymes responsible for the skin malfunction and aging process—collagenase, elastase, tyrosinase and hyaluronidase—was analysed. The research also included assessment of the in vitro photoprotection efficiency of honeybush extracts and their contribution to the wound healing process. Antioxidant properties of the prepared extracts were evaluated, and quantification of the main compounds in the extracts was achieved. The research showed that the analysed extracts had a significant ability to inhibit collagenase, tyrosinase and hyaluronidase and a weak influence on elastase activity. Tyrosinase was inhibited effectively by honeybush acetone (IC50 26.18 ± 1.45 µg/mL), ethanol (IC50 45.99 ± 0.76 µg/mL) and water (IC50 67.42 ± 1.75 µg/mL) extracts. Significant hyaluro...
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Molecules
Cinnamic acid is a plant metabolite with antimicrobial, anticancer, and antioxidant properties. I... more Cinnamic acid is a plant metabolite with antimicrobial, anticancer, and antioxidant properties. Its synthetic derivatives are often more effective in vitro than parent compounds due to stronger biological activities. In our study, we synthesized ten new N–(4–chloro–2–mercapto–5–methylphenylsulfonyl)cinnamamide derivatives, containing two pharmacophore groups: cinnamic acid moiety and benzenesulfonamide. The antimicrobial activity of the obtained compounds was estimated using different types of Gram-positive and Gram-negative bacteria, fungus species of Candida albicans, as well as clinical strains. The compounds were evaluated on biofilm formation and biofilm formed by Staphylococcus clinical strains (methicillin–resistance S. aureus MRSA and methicillin–resistance coagulase–negative Staphylococcus MRCNS). Furthermore, blood bacteriostatic activity test was performed using S. aureus and S. epidermidis. In cytotoxic study, we performed in vitro hemolysis assay on domestic sheep perip...
European Journal of Pharmaceutical Sciences, 2019
Comparison of the in vitro cytotoxicity among phospholipid-based parenteral drug delivery systems... more Comparison of the in vitro cytotoxicity among phospholipid-based parenteral drug delivery systems: Emulsions, liposomes and aqueous lecithin dispersions (WLDs).
Antioxidants
Mangiferin is a strong antioxidant that presents a wide range of biological activities. The aim o... more Mangiferin is a strong antioxidant that presents a wide range of biological activities. The aim of this study was to evaluate, for the first time, the influence of mangiferin on tyrosinase, an enzyme responsible for melanin synthesis and the unwanted browning process of food. The research included both the kinetics and molecular interactions between tyrosinase and mangiferin. The research proved that mangiferin inhibits tyrosinase activity in a dose-dependent manner with IC50 290 +/− 6.04 µM, which was found comparable with the standard kojic acid (IC50 217.45 +/− 2.54 µM). The mechanism of inhibition was described as mixed inhibition. The interaction between tyrosinase enzyme and mangiferin was confirmed with capillary electrophoresis (CE). The analysis indicated the formation of two main, and four less significant complexes. These results have also been supported by the molecular docking studies. It was indicated that mangiferin binds to tyrosinase, similarly to L-DOPA molecule, b...
Postepy Fitoterapii, Jan 30, 2008
Molecules, 2021
Polyphenolic compounds—mangiferin and hesperidin—are, among others, the most important secondary ... more Polyphenolic compounds—mangiferin and hesperidin—are, among others, the most important secondary metabolites of African shrub Cyclopia sp. (honeybush). The aim of this study was to compare the percutaneous absorption of mangiferin and hesperidin from solutions (water, ethanol 50%, (v/v)) and extracts obtained from green and fermented honeybush (water, ethanol 50%, (v/v)). Research was performed with the Bronaugh cells, on human dorsal skin. The mangiferin and hesperidin distributions in skin layers (stratum corneum, epidermis, and dermis) and in acceptor fluid (in every 2, 4, 6, and 24 h) were evaluated by HPLC–Photodiode Array Coulometric and Coulometric Electrochemical Array Detection. The transdermal distribution of hesperidin was also demonstrated by fluorescence microscopy. Results indicated that mangiferin and hesperidin were able to cross the stratum corneum and penetrate into the epidermis and dermis. An advantage of hesperidin penetration into the skin from the water over e...
BioTechniques, 2016
The xCELLigence Real-Time Cell Analyzer (RTCA) is a non-invasive, impedence-based biosensor syste... more The xCELLigence Real-Time Cell Analyzer (RTCA) is a non-invasive, impedence-based biosensor system that can measure cell viability, migration, growth, spreading, and proliferation. Changes in cell morphology and behavior are continuously monitored in real time using microelectronics located in the wells of RTCA E-plates. According to the manufacturer's recommendation, E-plates are single-use and disposable. Here, we show that E-plates can be regenerated and reused several times without significantly effecting experimental results.
Scientific Reports, 2016
Hypoxia-inducible factors (HIF) are heterodimeric transcription factors that allow cells to adapt... more Hypoxia-inducible factors (HIF) are heterodimeric transcription factors that allow cells to adapt and survive during hypoxia. Regulation of HIF1A and HIF2A mRNA is well characterized, whereas HIF3A mRNA regulation and function are less clear. Using RNA-Seq analysis of primary human umbilical vein endothelial cells, we found two isoforms of HIF3A were expressed, HIF3A2 and HIF3A3. Comparing HIF3A expression profiles to HIF1A mRNA during 48 hours of hypoxia revealed that HIF1A message peaked at 4 hours, whereas HIF3A expression increased while HIF1A was decreasing. Given that HIF1A mRNA is regulated by miR-429, we tested miR-429 effects on both HIF3A isoforms and found that they too were regulated by miR-429. Analysis of a HIF-3 target, DNA-damage-inducible transcript 4, a key survival gene, indicated that DDIT4 mRNA is induced by HIF-3 and negatively regulated by miR-429 through miR-429's actions on HIF3A message. This provides a compelling model for how hypoxiainduced miR-429 regulates the switch between HIF-1 adaptive responses to HIF-3 survival responses by rapidly decreasing HIF1A levels while simultaneously slowing the progression of HIF3A expression until the miR-429 levels drop below normoxic levels. Since HIF-1 drives HIF3A and miR-429 expression, this establishes a regulatory network in which miR-429 plays a pivotal role.
Chromatographia, 1990
The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ioniza... more The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ionization potentials but different molecular polarizability values was investigated using reversed-phase HPLC. Methanol-water binary systems containing [3-cyclodextrin were applied as the mobile phase. The relationships between the capacity factors, molecular polarizabilities and the shape parameter of solute molecules are discussed.
Chromatographia, 1990
The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ioniza... more The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ionization potentials but different molecular polarizability values was investigated using reversed-phase HPLC. Methanol-water binary systems containing [3-cyclodextrin were applied as the mobile phase. The relationships between the capacity factors, molecular polarizabilities and the shape parameter of solute molecules are discussed.
Chromatographia, 1989
The retention behaviour of steroids was investigated using normal and reversed-phase high-perform... more The retention behaviour of steroids was investigated using normal and reversed-phase high-performance TLC systems. Homologous series of esters and n-alcohols were applied as the mobile phases. The electrostatic interactions operating in these chromatographic systems were analysed by studing the relationship between the log (1/R F) values and the molecular polarizabilities of the solvents.
Canadian Journal of Plant Pathology, 2002
... to that of viscotoxins have been reported for cardiotoxins, cyto-toxins, or lytic factors fro... more ... to that of viscotoxins have been reported for cardiotoxins, cyto-toxins, or lytic factors from snake venoms (Samuelson 1974; Franz 1986; Urech et al. ... Bohlmann, H., Clauser, S., Behnke, S., Giese, H., Hiller, C., Reimann-Philipp, U., Schrader, G., Barkholt, V., and Apel, K. 1988. ...
Acta Biologica Cracoviensia s. Botanica, 2016
Rhododendron tomentosumHarmaja (formerlyLedum palustreL.) is a medicinal peat bog plant native to... more Rhododendron tomentosumHarmaja (formerlyLedum palustreL.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in EuropeR. tomentosumis classified as an endangered species, mainly due to degradation of peatlands. In the present work, the micropropagation protocol forR. tomentosumwas established for the first time, providing both anex situconservation tool and a means of continuous production ofin vivoandin vitroplant material for further studies.R. tomentosummicroshoots were initiated from leaf explants and further multiplied using Schenk-Hildebrandt (SH) medium supplemented with 9.84 μM 2iP and 1.00 μM TDZ. The shoots were elongated on the SH medium supplemented with 24.6 μM 2iP and subsequently rooted using the perlite substrate saturated with half-strength Woody Plant med...
Scientific Reports, 2016
Hypoxia-inducible factors (HIF) are heterodimeric transcription factors that allow cells to adapt... more Hypoxia-inducible factors (HIF) are heterodimeric transcription factors that allow cells to adapt and survive during hypoxia. Regulation of HIF1A and HIF2A mRNA is well characterized, whereas HIF3A mRNA regulation and function are less clear. Using RNA-Seq analysis of primary human umbilical vein endothelial cells, we found two isoforms of HIF3A were expressed, HIF3A2 and HIF3A3. Comparing HIF3A expression profiles to HIF1A mRNA during 48 hours of hypoxia revealed that HIF1A message peaked at 4 hours, whereas HIF3A expression increased while HIF1A was decreasing. Given that HIF1A mRNA is regulated by miR-429, we tested miR-429 effects on both HIF3A isoforms and found that they too were regulated by miR-429. Analysis of a HIF-3 target, DNA-damage-inducible transcript 4, a key survival gene, indicated that DDIT4 mRNA is induced by HIF-3 and negatively regulated by miR-429 through miR-429's actions on HIF3A message. This provides a compelling model for how hypoxia-induced miR-429 regulates the switch between HIF-1 adaptive responses to HIF-3 survival responses by rapidly decreasing HIF1A levels while simultaneously slowing the progression of HIF3A expression until the miR-429 levels drop below normoxic levels. Since HIF-1 drives HIF3A and miR-429 expression, this establishes a regulatory network in which miR-429 plays a pivotal role.
Chromatographia, 1991
The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ioniza... more The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ionization potentials but different molecular polarizability values was investigated by reversed-phase HPLC and gas chromatography, using 13-Cyclodextrin as a selective inclusion reagent. In HPLC, the CYclodextrin was applied as an additive to the ethanolwater binary mobile phase, while in gas chromatography 13-Cyclodextrin served as the stationary phase coated on an inert support. The relationships between the capacity factors, molecular polarizabilities and the shape parameter of Solute molecules is discussed.
Chromatographia, Feb 1, 1991
The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ioniza... more The retention behaviour of a group of polycyclic aromatic hydrocarbons having nearly equal ionization potentials but different molecular polarizability values was investigated by reversed-phase HPLC and gas chromatography, using 13-Cyclodextrin as a selective inclusion reagent. In HPLC, the CYclodextrin was applied as an additive to the ethanolwater binary mobile phase, while in gas chromatography 13-Cyclodextrin served as the stationary phase coated on an inert support. The relationships between the capacity factors, molecular polarizabilities and the shape parameter of Solute molecules is discussed.
Planta Medica, Aug 1, 2006
Chromatographia, 1994
A procedure is described for simultaneous determination of estriol, estrone and 1713-estradiol in... more A procedure is described for simultaneous determination of estriol, estrone and 1713-estradiol in human urine. After acid hydrolysis of the sulphate conjugates, the estrogens were extracted into diethyl ether. The ether extracts were concentrated and applied directly to an HPLC column. Using a 25 cm C-18 column and acetonitrile-water modified by the addition of [3cyclodextrin as mobile phase, the separation of estriol, estradiol and estrone was achieved within 20 minutes. The extraction of estrogens from the biological matrix is excellent. Estrogens were detected using a UVdetector (280 nm) or a spectrofluorimetric detector (~'exc = 280 nm, ~'em = 312 nm). The latter detection system has been used for the determination of estrogens in the urine of non-pregnant women. The procedure is simple and can be used in clinical practice.
Pharmaceutical Biology, Sep 28, 2008
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Papers by Jadwiga Renata Ochocka