Papers by JOHNNY ALDO TINCO JAYO
Pharmacognosy Journal, May 4, 2022
Objective: The current study aims to evaluate the hypoglycemic effect of the hydroalcoholic extra... more Objective: The current study aims to evaluate the hypoglycemic effect of the hydroalcoholic extract of Geranium ruizii on alloxan-induced hyperglycemia in albino mice. Material and Methods: Geranium ruizii was collected in Huancayo, Junin, Peru. A phytochemical analysis was carried out to confirm the chemical groups. In the pharmacological study, hyperglycemia was induced with alloxan at doses of 170 mg/kg in male albino mice, animals with blood sugar levels above 250 mg/dL were included in the protocol. Mice were randomized into five groups (I: Alloxan 170 mg/Kg; II, III and IV (Geranium ruizii extract: 50; 150 and 300 mg/Kg body weight); and V: glibenclamide, 5mg/Kg B.W. Results: Phytochemical analysis confirmed the presence of tannins, flavonoids, alkaloids, terpenes, saponins and phenolic compounds. It is observed that at doses of 50, 150 and 300 mg/kg reduced blood glucose at 14 days of treatment. Conclusion: The hypoglycemic effect of the hydroalcoholic extract of Geranium ruizii, administered orally in mice with alloxan-induced hyperglycemia was effective with 150 mg/Kg body weight.
International Research Journal of Pharmacy, 2015
The methanolic and aqueous extracts of dried wild edible mushroom were analyzed for phytochemical... more The methanolic and aqueous extracts of dried wild edible mushroom were analyzed for phytochemical screening, antibacterial, antioxidant activity (DPPH) and total phenolic content. The preliminary phytochemical results showed the occurrence of active compounds such as carbohydrate, glycosides, phytosterols, phenol, tannins, flavanoids, alkaloid, terpenoids and saponins. The wild edible mushroom extracts showed varying degree ofinhibition on the test organisms (Escherichia coli, Staphylococcus aureus, Bacillus cereus and Salmonella typhi). P. ostreatus showed good antibacterial activities against all the microorganisms tested. P. ostreatus revealed a high phenolics concentration in methanolic extract (24.012 mg GAE/g) as compared to aqueous extracts (16.5468 mg GAE/g) and powerful antioxidant properties. The antioxidant activity of evaluated mushroom extracts gave positive results with free radical scavenging activity. The result obtained from this study has shown the potential of mushroom extract as a potent therapeutic agent and a food supplement. It could find applications in the prevention of free radical-related diseases as a source of bioactive compounds.
Repositorio de Tesis - UNMSM, 2012
Molecules, 2021
Jatropha macrantha Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditi... more Jatropha macrantha Müll Arg. L is also known as “huanarpo macho” and used in the Peruvian traditional medicine as an aphrodisiac and erectile dysfunction (ED). The aim of this study was to determine the phytochemical constituents in leaves and stems ethyl acetate fraction (LEAF and SEAF) of J. macrantha and to compare the antioxidant activity and the ameliorative effect on ketamine-induced erectile dysfunction in rats. The phytochemical constituents were determined by LC-ESI-MS/MS, the total phenolic compounds and total flavonoids (TPC and TF) by Folin-Ciocalteu and aluminum chloride, respectively. The antioxidant activity was determined by DPPH, ABTS, and FRAP assays. Experimental groups were divided as follows: I: negative control; II: positive control (ketamine at 50 mg/ kg/d); III: sildenafil 5 mg/kg; IV, V, VI: LEAF at 25, 50 and 100 mg/kg, respectively, and VII, VIII, IX: SEAF at 25, 50, and 100 mg/kg, respectively. The phytochemical analysis revealed the presence mainly of co...
Pharmacognosy Journal, 2020
MATERIALS AND METHODS Chemicals 7,12-Dimethylbenz[a]anthracene (DMBA), Folin Ciocalteu reagent (F... more MATERIALS AND METHODS Chemicals 7,12-Dimethylbenz[a]anthracene (DMBA), Folin Ciocalteu reagent (FCR), gallic acid standard, minimal essential medium, fetal bovine serum, Dulbecco's modified Eagle's medium, dimethyl sulfoxide (DMSO), sulforhodamine B (SRB) and 5-flurouracil were obtained from Sigma Chemical Co. Kits for hematology and biochemical test were purchased from Quimica Suiza S.A. Other reagents were of chemical grade for analysis. Cell lines The cell lines MCF-7 (cancer) and 3T3 (mouse embryonic fibroblasts) were purchased from the American Type Culture Collection (ATCC,
Annual Research & Review in Biology, 2017
Aims: Many herbal products could have serious side effects. This study aim is to evaluate the in ... more Aims: Many herbal products could have serious side effects. This study aim is to evaluate the in vivo acute and sub-acute oral toxicity of ethanol extract of Tanacetum parthenium (L) Sch. Bip. in mice and rats. Study Design: In acute study: a total of 10 female Balb/c mice (20-25 g) were divided into 2 groups (n=5) namely control and tested group which received ethanol extract of T. parthenium orally at
Pharmacognosy Journal, 2019
Each part of Neem tree has various medicinal properties. Non woody part of Neem such as leaf, bar... more Each part of Neem tree has various medicinal properties. Non woody part of Neem such as leaf, bark, oil, flowers, fruits and seed show great properties that is Antiallergic, Antifungal, Antibiotic, Antidermatic, Antibacterial, Antiinflammatory, Insecticidal, Larvicidal, Antimalarial, Antiulcer and other biological activities. 5 Some water soluble extract of Azadirachta Indica have great importance in hyperglycemia, hypolipidemia and hypotensive activities. 6 ANTIMICROBIAL ACTIVITY OF NEEM Neem has great Antimicrobial activity it contains 35 biological active compounds. Neem leaf juice and twigs are used to clean teeth and used as a tonic and people of India used to place Neem leaves in their beds, books and cupboards to prevent bugs. 7 A number of potent pharmaceutical compounds limnoods and triterpenoids have been isolated from the fruits and bark of neem tree. Neem extracts and its different constituents play essential role in the inhibition of several microbes which includes viruses, fungi and bacteria. The extracts of methanol and hexane chloroform of Azadirachta indica were selected against antibacterial activity on Escherichia coli, Proteus vulgaris, Klebsiella pneumonia, Bacillus subtilis, Micrococcus luteus, Streptococcus faecalis and Enterococcus faecalis. It was revealed that methanol extract was the most effective, chloroform reasonably effective and hexane extract showed little antibacterial activity. 8 The antibacterial activity of neem describes as fellow. Antibacterial action of neem Neem usually used in medicine and pharmaceutics. The stem and bark of Neem has great antibacterial activity against Klebsiella, Serratia species and Streptococcus. 9 The methanolic extracts of Neem has antibacterial activity against Vibrio cholera and chloroform extracts against E. coli, Bacillus subtilis, Enterococcus faecalis and Streptococcus faecalis. 10 ABSTRACT Neem has become valuable plant in the world which shows the solutions for hundreds to thousands problems. Azadirachta indica (Neem) is a rapidly growing evergreen well known tree found Pada generally in various regions of world like America, Africa and India. It has been widely used in Chinese, Ayurveda and Unani medicines across the world especially in Asians countries for the prevention and treatment of diseases. The different parts of neem plant contain biological compounds responsible for antibacterial, antiviral and antifungal activities. It is considered as safe medicinal plants and modulates the numerous biological processes without any adverse effect. Neem tree produces some active compounds which contain biological activities, parts of neem tree such as Root, bark, leaf, flower, seed and fruit together possesses biological activities. Various compounds have been obtained from various parts of neem. Biological activities of few of them have been studied. Hence, the article is aims to utilize the medicinal properties of whole neem plant in various disorders of mankind.
Pharmacognosy Journal, 2019
Background: Experimental studies have demonstrated that fatty acids such as omega 3 and 6 have an... more Background: Experimental studies have demonstrated that fatty acids such as omega 3 and 6 have anti-inflammatory, anticonvulsant, cardio protective and anti-tumor effects. Objective: The main objective was to determine the protective effect of sacha inchi oil on pentylenetetrazoleinduced seizures in albino mice. Material and Methods: Thirty male Balb/C albino mice of 8 weeks of age were purchased from the National Institute of Health (Lima-Peru). The animals were divided into 5 groups of 6 animals each one. Group I: Control; PTZ (pentylenetetrazole 80 mg / kg, s.c.). II: PTZ + Diazepam (1 mg / kg; s.c.). Group III, IV, V: PTZ + sacha inchi oil at single doses of 250, 500, 1000 mg / kg respectively orally. Sacha inchi oil was administered 30 minutes before induction of seizures by PTZ. Next, various parameters such as latency, seizure frequency, duration and score were scored according to Racine scale. The program Graph Pad Prism v.4 was used for statistical analysis. Results: As result, the experimental group treated with sacha inchi oil at maximum dose of 1000 mg/kg showed better results in response to PTZ induced seizure; low levels of seizures, frequency and duration (P<0.001; ANOVA, P <0.001 Post-hoc Dunnett test). Conclusion: It is concluded that Sacha inchi oil presented anticonvulsant effect at the highest doses tested being very similar to Diazepam. The main mechanism could be by reducing free radical and improving GABA levels in the brain.
OncoTargets and Therapy, 2019
Toxics, 2019
Piper aduncum, commonly known as matico, is a plant that grows in the mountainous and coastal reg... more Piper aduncum, commonly known as matico, is a plant that grows in the mountainous and coastal regions of Peru, and is studied for its antimicrobial properties and various ethnopharmacological uses. The main objective of this study was to determine the cytoprotective and antioxidant effects of the methanolic extract of Piper aduncum leaves in Mus musculus previously administered with sodium fluoride (NaF) using the Micronucleus test and the Comet assay. The extract was administrated orally in four different concentrations: 150, 300, 600, and 1200 mg/Kg for ten days. At the 11th day, a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was performed with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according to OECD guidelines 474 and 489, respectively. The statistical analysis was performed by median analysis with ANOVA. Significant differences were found in Micronucleus frequ...
Asian Pacific Journal of Tropical Disease, 2017
contract N° 311-OGPIEC-UNICA-2014. The journal implements double-blind peer review practiced by s... more contract N° 311-OGPIEC-UNICA-2014. The journal implements double-blind peer review practiced by specially invited international editorial board members.
Universidad Nacional de San Cristóbal de Huamanga, 2005
TesisSe realizó un estudio explicativo, comparativo y prospectivo, con un diseño de pre y post pr... more TesisSe realizó un estudio explicativo, comparativo y prospectivo, con un diseño de pre y post prueba para la intervención farmacéutica en suministro de medicamentos. Probabilístico, conglomerado y polietápico para los clientes que acuden a estos establecimientos. La intervención se realizó en la Red Huamanga, tomando los 20 establecimientos que se encuentran en los respectivos comités locales de salud. Se usó el manual de intervención en suministro de medicamentos y las técnicas de encuesta con la escala de Lickert. Se encontró que sólo 15% de los responsables del medicamento trabajan en estos establecimientos, el 85% del cargo que tienen es diferente al nombramiento que tienen, edad promedio de 38 a 47 años, y el tiempo que vienen laborando es de 3 a 8 años. El 85% de los establecimientos no tienen Comité Fannacológico, las enfermedades más frecuentes son las EDAs, IRAs y desnutrición
Clinical and Experimental Gastroenterology, 2017
Background Currently, gastric cancer (GC) is considered a public health problem worldwide. Using ... more Background Currently, gastric cancer (GC) is considered a public health problem worldwide. Using medicinal plants for the prevention of chronic diseases such as cancer constitutes new alternatives in traditional medicine. Oenothera rosea (OR) could be an option, but it needs to be evaluated. Aim The main objective of this study was to evaluate the protective effect of OR extract on N-methyl-N-nitrosourea (NMU)-induced GC in rats. Methods In total, 80 male Holtzman rats were randomized into five groups. Group A received the saline solution (5mL/kg), group B received NMU 500 μg/kg (cancer inductor) by oral administration for 16 weeks, and groups C, D, and E were treated with OR extract (100, 200, and 300 mg/kg, respectively) and NMU in order to evaluate the preventive effect on cancer induced by NMU for 16 weeks. Blood and histological samples of stomachs were collected to determine histopathological, biochemical, and hematological parameters between different experimental groups. Res...
Plants
Quinoa has acquired a great interest due to its high content of nutrients and biomolecules that h... more Quinoa has acquired a great interest due to its high content of nutrients and biomolecules that have nutritional and medicinal properties. The aim of this study was to compare the total phenolic content (TPC), total flavonoids (TF), and the antioxidant capacity of 20 varieties of seeds and sprouts of quinoa extract. Quinoa seeds were germinated for 72 h and dried in an oven at 45 °C. The extracts were obtained by dynamic extraction using methanol. Phytochemical analysis with liquid chromatography coupled with mass spectrometry (LC-ESI-MS/MS), TPC, TF, and the antioxidant capacity was carried out and compared between both extracts. The TPC was determined with Folin-Ciocalteu reagent, TF with AlCl3, and the antioxidant capacity was determined according to the DPPH and ABTS assays. Sprout extracts showed high values of TPC (31.28 ± 0.42 mg GAE/g; Pasankalla variety), TF (14.31 ± 0.50 mg EQ/g; black Coito variety), and antioxidant capacity (IC50 (DPPH): 12.69 ± 0.29 µg/mL and IC50 (ABTS...
… , and Vascular Biology, 1995
The effect of 17 alpha-dihydroequilin sulfate (DHES), a water-soluble estrogen of conjugated estr... more The effect of 17 alpha-dihydroequilin sulfate (DHES), a water-soluble estrogen of conjugated estrogens (Premarin), and ethynylestradiol (EE), a commonly used estrogen found in many oral contraceptives, on the development of atherosclerosis was studied in rabbits fed an atherogenic diet (0.2% cholesterol) for 24 weeks. Ten animals were given 15 micrograms. kg-1.d-1 EE, 10 received 3.8 mg.kg-1.d-1 of DHES, and the remaining 10 sham-ovariectomized and 10 ovariectomized animals served as cholesterol-fed controls. These doses were chosen to have similar estrogenic potency. Plasma cholesterol concentrations increased to about 900 mg/dL and did not differ among the experimental groups. After 24 weeks, plasma beta-VLDL and HDL cholesterol concentrations were the same for all cholesterol-fed groups, while LDL cholesterol was significantly higher in the two estrogen-treated groups. In spite of this, both EE and DHES significantly reduced atherosclerosis by 35% in the aortic arch and 75% to 80% in the thoracic and abdominal aorta. The reduction in atherosclerosis was seen in animals with a wide range (400 to 1400 mg/dL) of plasma cholesterol concentrations and was independent of lipoprotein profile. beta-VLDL isolated from estrogen-treated animals was not significantly different from control beta-VLDL in its ability to stimulate cholesterol accumulation in THP-1 macrophages in culture. This suggests that the protective effect of estrogens on the development of atherosclerosis is not mediated by qualitative differences in beta-VLDL that affect uptake by macrophages. The results of this study extend our knowledge of the range of estrogens that reduce atherosclerosis. Given the lack of effect on plasma lipid and lipoprotein concentrations, these data are consistent with the conclusion that estrogens exert some of this beneficial effect directly at the level of the arterial wall by influencing certain key components in the pathogenesis of atherosclerosis.
Uploads
Papers by JOHNNY ALDO TINCO JAYO