Papers by Jérome Guicheux
Le secteur des aliments santé représente plus que jamais une manne pour les industriels, avec un ... more Le secteur des aliments santé représente plus que jamais une manne pour les industriels, avec un marché mondial de 11 milliards de dollars, en croissance de 5 % par an. Parallèlement, le marché des compléments alimentaires dépasse désormais les 50 milliards, malgré les restrictions imposées ces dernières années par la réglementation. Dans un tel contexte et parce que les exigences des consommateurs concernant les ingrédients et leur efficacité sont plus fortes, l'innovation reste la clef de voûte de cette économie à très haute valeur ajoutée. C'est pourquoi, le colloque Molécules & Ingrédients Santé 2014 sera de nouveau l'occasion de rassembler recherche académique, développement et applications industrielles dans une logique de partenariats et de transferts. Pour la 5 ème fois, MIS réunira pendant 2 jours scientifiques industriels, chercheurs universitaires et experts pour faire le point sur les dernières avancées techniques, ainsi que sur les perspectives en termes de nouveaux actifs, de tests cliniques et de règlementation. Les conférences sélectionnées par le Comité Scientifique seront réparties en différentes sessions : actifs marins, procédés, prébiotiques & probiotiques, réglementation & essais cliniques, actifs végétaux. Les multiples pauses permettront aux participants de créer des contacts, d'échanger et de consulter les posters. Le Prix du Meilleur Poster sera décerné à l'issue d'une présentation orale de 5 d'entre eux. Dans l'attente de vous accueillir à Quimper, Patrice MOREL Coordinateur de MIS 2014 The health products field still represents a strong profitable industry with a US $11 billion global market and a 5 % annual growth. In the same time, dietary supplements turnover is now exceeding US $50 billion in spite of recent regulatory limitations. In this context and because of stronger requirements of consumers regarding the efficacy of ingredients, innovation remains the keystone of this high value-added economy. Once again, Health Molecules & Ingredients 2014 symposium will be the opportunity to bring together academic research, development and industrial applications in order to trigger partnerships and technology transfers. For the 5 th time, MIS will gather during 2 days industrial scientists, university researchers as well as experts to focus on last advances and future prospects in terms of new active ingredients, clinical tests and legislation. The symposium will be divided into sessions including conferences selected by the Scientific Committee: marine ingredients, processes, prebiotics & probiotics, legislation & clinical trials, plant ingredients.
International Journal of Environmental Research and Public Health
Some patients with moderate haemophilia (PWMH) report joint damage potentially responsible for ga... more Some patients with moderate haemophilia (PWMH) report joint damage potentially responsible for gait disorders. Three-dimensional gait analysis (3DGA) is a relevant tool for the identification of complex musculoskeletal impairment. We performed an evaluation with 3DGA of 24 PWMH aged 44.3 ± 16.1 according to their joint status [Haemophilia Joint Health Score (HJHS) < 10 or HJHS ≥ 10] and assessed the correlation with the radiological and clinical parameters. Sixteen had HJHS < 10 (group 1) and eight had HJHS ≥ 10 (group 2). They were compared to 30 healthy subjects of a normative dataset. Both knee and ankle gait variable scores were increased in group 2 compared to the controls (p = 0.02 and p = 0.04, respectively). The PWMH of group 2 had a significant increase in their stance phase, double support duration, and stride width compared to the controls and group 1 (p < 0.01). Very low correlations were found for the ankle gait variable score with the ankle Pettersson sub-scor...
Le Centre pour la Communication Scientifique Directe - HAL - Inria, 2009
International audienc
European Spine Journal, 2020
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Cells, 2020
The founder cells of the Nucleus pulposus, the centre of the intervertebral disc, originate in th... more The founder cells of the Nucleus pulposus, the centre of the intervertebral disc, originate in the embryonic notochord. After birth, mature notochordal cells (NC) are identified as key regulators of disc homeostasis. Better understanding of their biology has great potential in delaying the onset of disc degeneration or as a regenerative-cell source for disc repair. Using human pluripotent stem cells, we developed a two-step method to generate a stable NC-like population with a distinct molecular signature. Time-course analysis of lineage-specific markers shows that WNT pathway activation and transfection of the notochord-related transcription factor NOTO are sufficient to induce high levels of mesendoderm progenitors and favour their commitment toward the notochordal lineage instead of paraxial and lateral mesodermal or endodermal lineages. This study results in the identification of NOTO-regulated genes including some that are found expressed in human healthy disc tissue and highli...
ACS Biomaterials Science & Engineering, 2020
ACS Biomaterials Science & Engineering, 2019
Finding alternative strategies for the regeneration of craniofacial bone defects (CSDs), such as ... more Finding alternative strategies for the regeneration of craniofacial bone defects (CSDs), such as combining a synthetic ephemeral calcium phosphate (CaP) implant and/or active substances and cells, would contribute to solving this reconstructive roadblock. However, CaP's architectural features (i.e., architecture and composition) still need to be tailored, and the use of processed stem cells and synthetic active substances (e.g., recombinant human bone morphogenetic protein 2) drastically limits the clinical application of such approaches. Focusing on solutions that are directly transposable to the clinical setting, biphasic calcium phosphate (BCP) and carbonated hydroxyapatite (CHA) 3D-printed disks with a triply periodic minimal structure (TPMS) were implanted in calvarial critical-sized defects (rat model) with or without addition of total bone marrow (TBM). Bone regeneration within the defect was evaluated, and the outcomes were compared to a standard-care procedure based on BCP granules soaked with TBM (positive control). After seven weeks, de novo bone formation was significantly greater in the CHA disks + TBM group than in the positive controls (3.33 and 2.15 mm 3 , respectively, P = 0.04). These encouraging results indicate that both CHA and TPMS architectures are potentially advantageous in the repair of CSDs and that this one-step procedure warrants further clinical investigation.
Cytotherapy, 2018
The Signature Series Symposium "Cellular Therapies for Orthopaedics and Musculoskeletal Disease P... more The Signature Series Symposium "Cellular Therapies for Orthopaedics and Musculoskeletal Disease Proven and Unproven Therapies-Promise, Facts and Fantasy" was held as a pre-meeting of the 26 th International Society for Cellular Therapy (ISCT) annual congress in Montreal, Canada, May 2, 2018. This was the first ISCT program that was entirely dedicated to the advancement of cell-based therapies for musculoskeletal diseases. Cellular therapies in musculoskeletal medicine are a source of great promise and opportunity. They are also the source of public controversy, confusion and misinformation. Patients, clinicians, scientists, industry and government share a commitment to clear communication and responsible development of the field. Therefore, this symposium convened thought leaders from around the world in a forum designed to catalyze communication and collaboration to bring the greatest possible innovation and value to patients with musculoskeletal conditions.
Acta biomaterialia, 2018
Articular cartilage is a connective tissue which does not spontaneously heal. To address this iss... more Articular cartilage is a connective tissue which does not spontaneously heal. To address this issue, biomaterial-assisted cell therapy has been researched with promising advances. The lack of strong mechanical properties is still a concern despite significant progress in three-dimensional scaffolds. This article's objective was to develop a composite hydrogel using a small amount of nano-reinforcement clay known as laponites. These laponites were capable of self-setting within the gel structure of the silated hydroxypropylmethyl cellulose (Si-HPMC) hydrogel. Laponites (XLG) were mixed with Si-HPMC to prepare composite hydrogels leading to the development of a hybrid interpenetrating network. This interpenetrating network increases the mechanical properties of the hydrogel. The in vitro investigations showed no side effects from the XLG regarding cytocompatibility or oxygen diffusion within the composite after cross-linking. The ability of the hybrid scaffold containing the compo...
Osteoarthritis and Cartilage, 2017
PLOS ONE, 2016
Introduction Chronic Periodontitis (CP) is an inflammatory disease of bacterial origin that resul... more Introduction Chronic Periodontitis (CP) is an inflammatory disease of bacterial origin that results in alveolar bone destruction. Porphyromonas gingivalis (Pg), one of the main periopathogens, initiates an inflammatory cascade by host immune cells thereby increasing recruitment and activity of osteoclasts, the bone resorbing cells, through enhanced production of the crucial osteoclastogenic factor, RANK-L. Antibodies directed against some cytokines (IL-1β, IL-6 and TNF-α) failed to exhibit convincing therapeutic effect in CP. It has been suggested that IL-33, could be of interest in CP. Objective the present study aims to analyze whether and how IL-33 and RANK-L and/or their interplay are involved in the bone destruction associated to CP.
Journal of endodontics, Jan 15, 2016
The dentin extracellular matrix is a reservoir of bioactive molecules sequestered into dentin dur... more The dentin extracellular matrix is a reservoir of bioactive molecules sequestered into dentin during dental initial development. They can be released under pathological conditions but also by controlled demineralization with bioactive materials. The purpose of this study was to investigate the ability of a biomedical hydrogel to extract and release these proteins from smashed dentin. Smashed dentin was obtained with 2 different kinds of grinders: a blade mill and a zirconia mortar grinder. The particle size was measured by scanning electron microscopy. Dentin powder was incorporated into a silated hydroxypropylmethylcellulose hydrogel. Several types of mixtures with variable parameters were tested. The mixtures were immersed into phosphate-buffered saline. The supernatants were collected, and the total released proteins were quantified by gel shift migration and Coomassie staining. The presence of transforming growth factor beta 1 was investigated by Western blot analysis and the EL...
Articular cartilage does not repair itself spontaneously. To promote its repair, the transfer of ... more Articular cartilage does not repair itself spontaneously. To promote its repair, the transfer of stem cells from adipose tissue (ATSC) using an injectable self-setting cellulosic-hydrogel (Si-HPMC) appears promising. In this context, the objective of this work was to investigate the influence of in vitro chondrogenic differentiation of ATSC on the in vivo cartilage formation when combined with Si-HPMC. In a first set of experiments, we characterized ATSC for their ability to proliferate, self renew and express typical mesenchymal stem cell surface markers. Then, the potential of ATSC to differentiate towards the chondrogenic lineage and the optimal culture conditions to drive this differentiation were evaluated. Real-time RT-PCR and histological analysis for sulphated glycosaminoglycans and type II collagen revealed that 3-dimensional culture and hypoxic condition favored ATSC chondrogenesis regarding mRNA expression level and the corresponding proteins production. In order to assess the phenotypic stability of chondrogenically-differentiated ATSC, real-time RT-PCR for specific terminal chondrogenic markers and alkaline phosphatase activity assay were performed. In addition to promote chondrogenesis, our culture conditions seem to prevent the terminal differentiation of ATSC. Histological examination of ATSC/Si-HPMC implants suggested that the in vitro chondrogenic pre-commitment of ATSC in monolayer is sufficient to obtain cartilaginous tissue in vivo.
Rheumatology, 2009
Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) ... more Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) cell biology by comparing the phenotype of nucleus pulposus (NP) and annulus fibrosus (AF) cells with that of articular chondrocytes (ACs). Methods. Rabbit cells from NP and AF were isolated and their phenotype was compared with that of AC by real-time PCR analysis of type I (COL1A1), II (COL2A1) and V (COL5A1) collagens, aggrecan transcript (AGC1), matrix Gla protein (MGP) and Htra serine peptidase 1 (Htra1). Results. Transcript analysis indicated that despite certain similarities, IVD cells exhibit distinct COL2A1/COL1A1 and COL2A1/AGC1 ratios as compared with AC. The expression pattern of COL5A1, MGP and Htra1 makes it possible to define a phenotypic signature for NP and AF cells. Conclusions. Our study shows that NP and AF cells exhibit a clearly distinguishable phenotype from that of AC. Type V collagen, MGP and HtrA1 could greatly help to discriminate among NP, AF and AC cells.
Progress in Solid State Chemistry, 2006
A new process for preparing oligonucleotide arrays is described that uses surface grafting chemis... more A new process for preparing oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. On the other hand, the reaction of a bisphosphonate bone resorption inhibitor (Zoledronate) with calcium deficient hydroxyapatites (CDAs) was studied as a potential route to local drug delivery systems active against bone resorption disorders. A simple mathematical model of the Zoledronate / CDA interaction was designed that correctly described the adsorption of Zoledronate onto CDAs. The resulting Zoledronate-loaded materials were found to release the drug in different phosphate-containing media, with a satisfactory agreement between experimental data and the values predicted from the model.
Macromolecular Symposia, 2008
We have developed a self-reticulating polymer based on silanized hydroxypropylmethylcellulose (Si... more We have developed a self-reticulating polymer based on silanized hydroxypropylmethylcellulose (Si-HPMC). The aim of this study was to determine whether this Si-HPMC hydrogel with or without calcium phosphate granules could represent a potential scaffold for bone tissue engineering. This study showed that Si-HPMC hydrogel didn't affect SaOS-2 and rat bone marrow cells viability. In addition, SaOS-2 cells are able to proliferate within Si-HPMC hydrogel containing or not calcium phosphate granules whereas Rat bone marrow cells proliferate only at the surface of calcium phosphate granules contained within Si-HPMC hydrogel. Finally, SaOS-2 cells seeded at the surface of reticulated Si-HPMC were not able to penetrate the hydrogel, while J774, a macrophage cells line, were able to move into the Si-HPMC hydrogel. These data indicate that Si-HPMC is a promissing scaffold for tissue engineering.
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Papers by Jérome Guicheux