Papers by Ivaylo Stefanov
Biomacromolecules, 2017
The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. D... more The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes, and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic crosslinking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress and bacterial contamination. In vitro studies revealed above 90 % antioxidant activity, and MPO and collagenase inhibition by up to 98 and 23 %, respectively. Ex vivo studies with venous leg ulcer exudates confirmed the inhibitory capacity of the dressings against MPO and MMPs. Additionally, the hydrogels reduced the population of the most frequently encountered in non-healing wounds bacterial strains. The stable at physiological conditions and resistant to lysozyme degradation hydrogels showed high biocompatibility with human skin fibroblasts.
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Papers by Ivaylo Stefanov