Papers by Isabel Peraleda Diaz
Plant Cell Reports, 1999
The effect of dithiothreitol (DTT) on the expression of the /3-glucuronidase (GUS) repórter gene ... more The effect of dithiothreitol (DTT) on the expression of the /3-glucuronidase (GUS) repórter gene under the control of the CaMV-35 S promoter has been investigated by radioactive labelling and immunoprecipitation of the enzyme in protoplasts from stably transformed tobáceo plants and compared with that observed in protoplasts transiently expressing the same gene construct. An increase in net accumulation of GUS during the culture period in response to externally added DTT (2 mM) was observed both in protoplasts from transformed tobáceo plants and in electroporated protoplasts. DTT had no effect on rate of degradation of the mature GUS protein, as shown in a pulse-chase experiment. Relevant aspects of protoplast physiology, such as viability, synthesis of 35 S-labelled cellular proteins, or synthesis and export of pathogenesis-related proteins (one putative chitinase and two l,3-/3-glucanases) were not affectedby the reducing reagent.
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Papers by Isabel Peraleda Diaz