Isolation of the methanol extract of Solanum xanthocarpum resulted in the isolation and character... more Isolation of the methanol extract of Solanum xanthocarpum resulted in the isolation and characterization of carpesterol (1) and four steroidal glycosides (2, 3, 4, and 5). The structures of these compounds were established by spectroscopic analysis. The antifungal activity of the steroidal compounds extracted from the fruits of S. xanthocarpum was investigated against Aspergillus niger and Trichoderma viride. The isolated compounds 1, 2, 3, 4, and 5 exhibited inhibitory effects on the radial growth of A. niger and Trichoderma viride.
The proccessed substrates were pre-soaked in water for overnight and then partially pasteurized b... more The proccessed substrates were pre-soaked in water for overnight and then partially pasteurized by immersing them for 2 to 3hr in boiling water. Excess water was drain off to maintain 60-70% moisture content by squeezing with hands and then allowed to cool down for a certain period (1hr). The cooled substrate was then mixed with grain spawn and filled in perforated polythene bags (60 x 45 cm). Spawning was done by ‘layer spawning method ’ @ 100g/500g (dry wt.) of the substrate. After spawning, the mouth of the polythene bag was closed securely using a rubber band. For every treatment, 4 (four) replicates were kept. All the bags were then incubated in a warm dark room for spawn running. The temperature of the mushroom shed was maintained at 25-300C. The bags were cut open when the mycelial run was completed. Watering was done twice a day regularly and relative humidity (RH) was maintained at 80-90%. A total of 3 to 4 flushes were obtained within 45 days. Fresh weights of mushrooms we...
Insect species complex of cauliflower and biology of Pieris brassicae (L.) were studied during 20... more Insect species complex of cauliflower and biology of Pieris brassicae (L.) were studied during 2013–2014 in the Department of Life Sciences, Manipur University. The study revealed twenty five species of insects comprising of 16 pests and 8 predators (4 coccinellids, 3 syrphids and one crambid). The biology of Pieris brassicae (L.) on cauliflower revealed that the egg period was 3.00±0.00 and 4.0±0.00 days during October-December and January-April respectively. The entire life cycle (egg to adult) occupied 50.22±1.56 days and 60.76±1.71 days during the two seasons studied.
S. Nimyaola, M.Shyamkesho Singh and N. Irabanta Singh Centre of Advanced Study in Life Sciences, ... more S. Nimyaola, M.Shyamkesho Singh and N. Irabanta Singh Centre of Advanced Study in Life Sciences, Manipur University, Canchipur-795003, Imphal ...................................................................................................................... Manuscript Info Abstract ......................... ........................................................................ Manuscript History
The present investigation was carried out for understanding and exploring the indigenous phosphat... more The present investigation was carried out for understanding and exploring the indigenous phosphate solubilizing fungi and their efficient utilization as a potential biofertilizer for the improvement of soil fertility. For our study, the rhizospheric soils were collected from 21 different ginger fields of Rengkai, Churachandpur District,Manipur. Fungi were isolated from the rhizospheric soil samples. The dominant species were selected and screened for phosphate solubilizing activities using rock phosphate and tri-calcium phosphate in NBRIP Growth Medium. Fungi that showed halo zones around the colonies are good phosphate solubilizers and belong mainly to the genera of Aspergillus, Penicillium and Trichoderma. The present study would help in utilizing the indigenous phosphate solubilizing fungal strains as an effective biofertilizers by the farmers.
The antifungal activity of leaf extracts of plants of Azadirachta indica, Vitex negundo, Eupatori... more The antifungal activity of leaf extracts of plants of Azadirachta indica, Vitex negundo, Eupatorium birmanicum, Centella asiatica, Ageratum conyzoides, Gynura angulosa, Adhatoda vasica and Mentha aravensis was investigated for the control of fruit rot pathogen of Pineapple (Aspergillus niger) at different concentrations ( 5,10,15 and 20 %). At 20 % concentration , the maximum inhibition of the growth of the fungus was observed with the extracts of Eupatorium birmanicum, followed by the extracts of Vitex negundo. The minimum inhibition rate of the fungus was found in the leaf extracts of Adhatoda vasica at all the level of concentrations.
Varying levels of chemical fertilizers i.e. NPK and vermicompost significantly influenced the yie... more Varying levels of chemical fertilizers i.e. NPK and vermicompost significantly influenced the yield attributing characters and growth and biomass production in cabbage (Brassica oleraceae var.capitata) cv.
The study was conducted with ethanolic extract of e dibl mushroom, Lentinus connatus, which was t... more The study was conducted with ethanolic extract of e dibl mushroom, Lentinus connatus, which was tested for total phenol, flavonoid, β-carotene, lycopene and ascorbic acid and in vitro antioxidant activity in terms of superoxide anion and DPPH radical scavenging activity. Some ot her biochemical assays including chelating effect o f ferrous ion, reducing power and total antioxidant capacity assay were conducted with varying concentrations. The data showed that EC 50 values were between 1 – 2 mg /ml except superoxide an DPPH radical scavenging tests. Phenol was present in the highest amount followed by other bioactive components (flavonoids, ascorbic acid, β carotene and lycopene in the respective order). The results obtained reveal that L. connatus can be a potential source of natural antioxidant which may be used to treat vari ous oxidative stress related diseases.
Asian Journal of Pharmaceutical and Clinical Research, 2014
Objective: Aim of this work was to evaluate the antioxidant properties of the edible mushroom spe... more Objective: Aim of this work was to evaluate the antioxidant properties of the edible mushroom species, Lentinula edodes . Methods: Presence of potential antioxidant compounds, such as, ascorbic acid, β carotene, lycopene, phenolics and flavonoids were tested. To understand the antioxidant potentiality like scavenging of superoxide radical, chelating of ferrous ion, scavenging capacity of DPPH radicals and reducing power of the extract were investigated. Results: The extraction yield achieved from L. edodes extraction was 2.4%. Total phenolic content was 25.5 mg GAE/g and total flavonoids content was 2 mg CE/g. The EC 50 value for superoxide radical scavenging activity was found to be at a concentration of 0.64 mg/ml. Conclusion: The results obtained reveal that L. edodes can be a potential source of natural antioxidant which may be used to treat various oxidative stress related diseases. Key words: Ascorbic acid, edible mushroom, Lentinula edodes, flavonoids, phenols, reactive oxyge...
Systematic surveys of Parthenium hysterophorus L. infested regions on 12 selected national highwa... more Systematic surveys of Parthenium hysterophorus L. infested regions on 12 selected national highways of North-Eastern India were conducted during 2009–2011. In all the surveyed sites, Parthenium hysterophorus was present but its abundance was more during the month of June to August. Complete inhibition of Parthenium seed germination was recorded at 20% of leaf and stem extracts of Cassia sericea, Mimosa pudica and Cassia tora. Different stages of Zygograma bicolorata (grubs to adult) showed reduction in plant height, shoot length, root length and biomass of Parthenium when exposed in mosquito net cages.
Different biochemical reactions in our body generat free radicals and these are capable of damagi... more Different biochemical reactions in our body generat free radicals and these are capable of damaging crucial biomolecules. Mushrooms have the capacity t o destroy such free radicals formed in our body as they possess antioxidant properties. The purpose of this study was to determine the antioxidant activities of mushroom, Pleurotus cornucopiae collected from Eastern Himalayan region. Antioxida nt activity was determined using reducing power assay, DPPH radical scavenging assay, chelating effect of ferrous ion and total antioxidant capacity assay. Ethanolic fractio n from the above mentioned edible mushroom was also tested for total phenol, flavonoid, β-carotene, lycopene and ascorbic acid content. Find ings showed that most of the EC50 values were more than 2 mg /ml except DPPH radical s venging test. The extract exhibited 50% DPPH radical scavenging activity at only 1.5 mg /ml conc e tration. Estimated putative antioxidant component s was in order of phenol > ascorbic acid > f...
L'etude des champignons sur feuilles de Z.l. a ete effectuee durant l'annee 1983. Le nomb... more L'etude des champignons sur feuilles de Z.l. a ete effectuee durant l'annee 1983. Le nombre de genres identifies dans la phyllosphere des feuilles vertes est de 23 et de 19 pour les jaunes, tandis que sur le phylloplan il est, respectivement de 11 et 12. Quelques genres dominants se rencontrent dans la phyllosphere tandis que le phylloplan n'en presente pas. Melanopsychium esculentum (parasite de Z.l.) n'a ete rencontre que durant les mois de Janvier, Fevrier et Decembre
Isolation of the methanol extract of Solanum xanthocarpum resulted in the isolation and character... more Isolation of the methanol extract of Solanum xanthocarpum resulted in the isolation and characterization of carpesterol (1) and four steroidal glycosides (2, 3, 4, and 5). The structures of these compounds were established by spectroscopic analysis. The antifungal activity of the steroidal compounds extracted from the fruits of S. xanthocarpum was investigated against Aspergillus niger and Trichoderma viride. The isolated compounds 1, 2, 3, 4, and 5 exhibited inhibitory effects on the radial growth of A. niger and Trichoderma viride.
The proccessed substrates were pre-soaked in water for overnight and then partially pasteurized b... more The proccessed substrates were pre-soaked in water for overnight and then partially pasteurized by immersing them for 2 to 3hr in boiling water. Excess water was drain off to maintain 60-70% moisture content by squeezing with hands and then allowed to cool down for a certain period (1hr). The cooled substrate was then mixed with grain spawn and filled in perforated polythene bags (60 x 45 cm). Spawning was done by ‘layer spawning method ’ @ 100g/500g (dry wt.) of the substrate. After spawning, the mouth of the polythene bag was closed securely using a rubber band. For every treatment, 4 (four) replicates were kept. All the bags were then incubated in a warm dark room for spawn running. The temperature of the mushroom shed was maintained at 25-300C. The bags were cut open when the mycelial run was completed. Watering was done twice a day regularly and relative humidity (RH) was maintained at 80-90%. A total of 3 to 4 flushes were obtained within 45 days. Fresh weights of mushrooms we...
Insect species complex of cauliflower and biology of Pieris brassicae (L.) were studied during 20... more Insect species complex of cauliflower and biology of Pieris brassicae (L.) were studied during 2013–2014 in the Department of Life Sciences, Manipur University. The study revealed twenty five species of insects comprising of 16 pests and 8 predators (4 coccinellids, 3 syrphids and one crambid). The biology of Pieris brassicae (L.) on cauliflower revealed that the egg period was 3.00±0.00 and 4.0±0.00 days during October-December and January-April respectively. The entire life cycle (egg to adult) occupied 50.22±1.56 days and 60.76±1.71 days during the two seasons studied.
S. Nimyaola, M.Shyamkesho Singh and N. Irabanta Singh Centre of Advanced Study in Life Sciences, ... more S. Nimyaola, M.Shyamkesho Singh and N. Irabanta Singh Centre of Advanced Study in Life Sciences, Manipur University, Canchipur-795003, Imphal ...................................................................................................................... Manuscript Info Abstract ......................... ........................................................................ Manuscript History
The present investigation was carried out for understanding and exploring the indigenous phosphat... more The present investigation was carried out for understanding and exploring the indigenous phosphate solubilizing fungi and their efficient utilization as a potential biofertilizer for the improvement of soil fertility. For our study, the rhizospheric soils were collected from 21 different ginger fields of Rengkai, Churachandpur District,Manipur. Fungi were isolated from the rhizospheric soil samples. The dominant species were selected and screened for phosphate solubilizing activities using rock phosphate and tri-calcium phosphate in NBRIP Growth Medium. Fungi that showed halo zones around the colonies are good phosphate solubilizers and belong mainly to the genera of Aspergillus, Penicillium and Trichoderma. The present study would help in utilizing the indigenous phosphate solubilizing fungal strains as an effective biofertilizers by the farmers.
The antifungal activity of leaf extracts of plants of Azadirachta indica, Vitex negundo, Eupatori... more The antifungal activity of leaf extracts of plants of Azadirachta indica, Vitex negundo, Eupatorium birmanicum, Centella asiatica, Ageratum conyzoides, Gynura angulosa, Adhatoda vasica and Mentha aravensis was investigated for the control of fruit rot pathogen of Pineapple (Aspergillus niger) at different concentrations ( 5,10,15 and 20 %). At 20 % concentration , the maximum inhibition of the growth of the fungus was observed with the extracts of Eupatorium birmanicum, followed by the extracts of Vitex negundo. The minimum inhibition rate of the fungus was found in the leaf extracts of Adhatoda vasica at all the level of concentrations.
Varying levels of chemical fertilizers i.e. NPK and vermicompost significantly influenced the yie... more Varying levels of chemical fertilizers i.e. NPK and vermicompost significantly influenced the yield attributing characters and growth and biomass production in cabbage (Brassica oleraceae var.capitata) cv.
The study was conducted with ethanolic extract of e dibl mushroom, Lentinus connatus, which was t... more The study was conducted with ethanolic extract of e dibl mushroom, Lentinus connatus, which was tested for total phenol, flavonoid, β-carotene, lycopene and ascorbic acid and in vitro antioxidant activity in terms of superoxide anion and DPPH radical scavenging activity. Some ot her biochemical assays including chelating effect o f ferrous ion, reducing power and total antioxidant capacity assay were conducted with varying concentrations. The data showed that EC 50 values were between 1 – 2 mg /ml except superoxide an DPPH radical scavenging tests. Phenol was present in the highest amount followed by other bioactive components (flavonoids, ascorbic acid, β carotene and lycopene in the respective order). The results obtained reveal that L. connatus can be a potential source of natural antioxidant which may be used to treat vari ous oxidative stress related diseases.
Asian Journal of Pharmaceutical and Clinical Research, 2014
Objective: Aim of this work was to evaluate the antioxidant properties of the edible mushroom spe... more Objective: Aim of this work was to evaluate the antioxidant properties of the edible mushroom species, Lentinula edodes . Methods: Presence of potential antioxidant compounds, such as, ascorbic acid, β carotene, lycopene, phenolics and flavonoids were tested. To understand the antioxidant potentiality like scavenging of superoxide radical, chelating of ferrous ion, scavenging capacity of DPPH radicals and reducing power of the extract were investigated. Results: The extraction yield achieved from L. edodes extraction was 2.4%. Total phenolic content was 25.5 mg GAE/g and total flavonoids content was 2 mg CE/g. The EC 50 value for superoxide radical scavenging activity was found to be at a concentration of 0.64 mg/ml. Conclusion: The results obtained reveal that L. edodes can be a potential source of natural antioxidant which may be used to treat various oxidative stress related diseases. Key words: Ascorbic acid, edible mushroom, Lentinula edodes, flavonoids, phenols, reactive oxyge...
Systematic surveys of Parthenium hysterophorus L. infested regions on 12 selected national highwa... more Systematic surveys of Parthenium hysterophorus L. infested regions on 12 selected national highways of North-Eastern India were conducted during 2009–2011. In all the surveyed sites, Parthenium hysterophorus was present but its abundance was more during the month of June to August. Complete inhibition of Parthenium seed germination was recorded at 20% of leaf and stem extracts of Cassia sericea, Mimosa pudica and Cassia tora. Different stages of Zygograma bicolorata (grubs to adult) showed reduction in plant height, shoot length, root length and biomass of Parthenium when exposed in mosquito net cages.
Different biochemical reactions in our body generat free radicals and these are capable of damagi... more Different biochemical reactions in our body generat free radicals and these are capable of damaging crucial biomolecules. Mushrooms have the capacity t o destroy such free radicals formed in our body as they possess antioxidant properties. The purpose of this study was to determine the antioxidant activities of mushroom, Pleurotus cornucopiae collected from Eastern Himalayan region. Antioxida nt activity was determined using reducing power assay, DPPH radical scavenging assay, chelating effect of ferrous ion and total antioxidant capacity assay. Ethanolic fractio n from the above mentioned edible mushroom was also tested for total phenol, flavonoid, β-carotene, lycopene and ascorbic acid content. Find ings showed that most of the EC50 values were more than 2 mg /ml except DPPH radical s venging test. The extract exhibited 50% DPPH radical scavenging activity at only 1.5 mg /ml conc e tration. Estimated putative antioxidant component s was in order of phenol > ascorbic acid > f...
L'etude des champignons sur feuilles de Z.l. a ete effectuee durant l'annee 1983. Le nomb... more L'etude des champignons sur feuilles de Z.l. a ete effectuee durant l'annee 1983. Le nombre de genres identifies dans la phyllosphere des feuilles vertes est de 23 et de 19 pour les jaunes, tandis que sur le phylloplan il est, respectivement de 11 et 12. Quelques genres dominants se rencontrent dans la phyllosphere tandis que le phylloplan n'en presente pas. Melanopsychium esculentum (parasite de Z.l.) n'a ete rencontre que durant les mois de Janvier, Fevrier et Decembre
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