Papers by Ira Widjiastuti
Lactobacillus acidophilus obtained from dentin caries and produces organic acids resulting in dec... more Lactobacillus acidophilus obtained from dentin caries and produces organic acids resulting in decalcification of dentin. On the process of dentinogenesis is active but will decrease in secondary dentinogenesis. Invasion of the bacteria causing the onset of the immune response by forming odontoblast dentin reparative, indicated by an increased expression of DMP-1, but the mechanism of the formation of dentin is still unclear. The purpose of this study is to show the odontoblast cell like exposure to Lactobacillus acidophilus expression against NFκβ and DMP 1. Pulp tissue is taken from the third molar of teeth that have been extracted then exposed to Lactobacillus acidophilus and expression of NFκβ and DMP 1 measured using imunocytochemistry techniques. Results show an increased NFκβ expression and decrease DMP 1 expression in the odontoblast like cell in exposure Lactobacillus acidophilus. In conclusion, Lactobacillus acidophilus increases the expression of NFκβ and inhibits the expr...
Ekonomika ta derzhava, 2021
Indian Journal of Dental Research, 2020
Background: Calcium hydroxide [Ca(OH)2] is a material used during pulp capping treatment, despite... more Background: Calcium hydroxide [Ca(OH)2] is a material used during pulp capping treatment, despite being readily soluble in both water and acid. In contrast, propolis constitutes a nontoxic resin which is not easily dissolved in water. Therefore, a combination of Ca(OH)2and propolis is assumed to be capable of increasing the mechanical properties of Ca(OH)2and to diffuse into the dentinal tubules. Objective: This research aimed to reveal the solubility and water sorption ability of a combination of Ca(OH)2and propolis as pulp capping material. Materials and Method: The samples comprised 18 Ca(OH)2and Ca(OH)2-propolis chips, 15 mm × 1 mm in dimension, all of which were stored in an incubator for 24 h at 37°C. Each sample was then divided into two groups: one dissolved in 50 mL of artificial saliva for 24 h at 37°C and another for 7 days before being weighed, dried, incubated, and weighed for a second time. The result of the reduction in mass divided by the volume of the samples was considered to constitute the level of solubility and water sorption. The difference between the solubility and water sorption ability was analyzed using an independent t-test with significant difference <0.05. Results: The solubility of Ca(OH)2-propolis is lower than that of Ca(OH)2 after immersion for 1 day (P = 0.001) and 7 days (P = 0.000). The water sorption ability of Ca(OH)2-propolis is no different than that of Ca(OH)2after immersion for 1 day (P = 0.088) and 7 days (P = 0.635). However, the water sorption ability of Ca(OH)2-propolis after 1-day immersion is higher than immersion for 7 days (P = 0.012). Conclusion: The solubility Ca(OH)2-propolis is lower than that of Ca(OH)2, but its water sorption is higher than that of Ca(OH)2.
Dental Journal (Majalah Kedokteran Gigi), 2021
Background: According to Riset Kesehatan Dasar (Riskesdas) (2013) and the World Health Organisati... more Background: According to Riset Kesehatan Dasar (Riskesdas) (2013) and the World Health Organisation (WHO), caries is still a global problem and highly prevalent in Indonesia. Caries is mainly caused by Streptococcus mutans with virulence factors known as glucosyltransferase (GTF). The GTF enzyme contribute to the pathogenesis of caries by converting sucrose to fructose and glucan, which are then used in the formation of biofilms and dental plaques. Natural propolis compounds containing flavonoids, terpenoids, saponins and tannins, can inhibit GTF enzyme activity. Purpose: This study aimed to determine an effective concentration of propolis extract for inhibiting the S. mutans GTF enzyme activity. Methods: This study used propolis extract at 14 μg/ mL, 16 μg/m and 1 μg/mL to determine the inhibitory effect on S. mutans GTF enzyme activity. The GTF enzyme were obtained from the supernatant from S. mutans culture centrifugation. The GTF enzyme activity was measured using high-performan...
Conservative Dentistry Journal, 2021
Background: Dental caries is a hard tissue disease caused by bacterial metabolic activity that ca... more Background: Dental caries is a hard tissue disease caused by bacterial metabolic activity that causes demineralization, teeth that have caries should be filled in order to function again. One of the restorative materials closer to natural teeth is composite resin because it is the same colour as natural teeth and is easy to shape. As for the placement technique, bulkfill is also used because it is more time-efficient, and irradiation is accompanied by applying the restoration material in one batch. However, the bulkfill technique often causes micro-leakage of dental caries deposition. The intermediate layer is an intermediate layer used to prevent micro-leakage at the edges of the restoration as a base layer for restoration to create a good adaptation before applying packable composite resin. Therefore, bulkfill coating technique with the intermediate layer can reduce the risk of microleakage. Purpose: To describe the bulkfill technique with an intermediate layer against marginal ad...
F1000Research, 2020
Background:Caries in the dental pulp result in inflammation and damage to the pulp tissue. During... more Background:Caries in the dental pulp result in inflammation and damage to the pulp tissue. During inflammation of the pulp, various inflammatory mediators and growth factors are released, including IL-8, IL-10, TLR-2, VEGF and TGF-β through the NF-kB pathway. In the present study, therapy for pulpal caries was performed through pulp capping by giving a combination of propolis and calcium hydroxide (Ca(OH)2). This treatment was expected to stimulate the formation of reparative dentin as an anti-inflammatory material to prevent pulp tissue damage.Methods:28 Wistar rats were divided into four groups and treated with Ca(OH)2 with or without the addition of propolis for either 7 or 14 days. Immunohistochemical examination was used to determine the expression of IL-8, IL-10, TLR-2, VEGF, TGF-β in the four treatment groups.Results:The group treated with a combination of propolis and Ca(OH)2 for 7 days showed that the expression of IL-10, IL-8, TLR-2, VEGF, TGF-β increased significantly com...
Indian Journal of Forensic Medicine & Toxicology, 2021
Objective: Calcium hydroxide is widely used as pulp-capping material, but it is resistant to Aggr... more Objective: Calcium hydroxide is widely used as pulp-capping material, but it is resistant to Aggregatibacteractinomycetemcomitans bacteria. Because of this deficiency, many researchers are considering usingalternative natural ingredients, such as propolis. Propolis has been widely used in dentistry due to itsproperties and because it has active ingredients that can act as antibacterial agents. It is hoped that whencalcium hydroxide and propolis are combined, the combination can overcome the disadvantages. The aim ofthis study was to determine the dosage effectiveness of the combination calcium hydroxide-propolis as aninhibitor for A. actinomycetemcomitans biofilm. Material and methods: The sample was divided into fivegroups. The positive control group is a combination of calcium hydroxide-sterile aquadest, and the negativecontrol group is A. actinomycetemcomitans bacteria. Group I is a combination of calcium hydroxidepropolis with a ratio of 1:1, group II has a ratio of 1:1.5, and g...
Background: The number of failures in pulp capping treatment were still high, the cause of damage... more Background: The number of failures in pulp capping treatment were still high, the cause of damage in teeth was caries. The material that has been used for pulp capping causes tunnel defect and necrosis in pulp so an alternative material was required. Propolis was known to be containing resin and has anti-inflammatory properties which can be used as a material for pulp capping. Several researches have used propolis as a material for pulp capping but the mechanism as to how the propolis stimulated the formation of reparative dentin remained unclear.The objective of this study was : to reveal the molecular mechanism of propolis stimulation on odontoblast like cells exposed to inactive Lactobacillus acidophilus in inducing differentiation of pulp fibroblast. Methods: this research was done in pulp cell culture induced by inactive Lactobacillus acidophilus and exposed to propolis extract. Pulp cell culture isolated from human impacted thirds molar that has been extracted. Observation and...
Background: Necrotic teeth with periapical abscess is one of the most common problem in endodonti... more Background: Necrotic teeth with periapical abscess is one of the most common problem in endodontic. Management of necrotic tooth with a large periapical abscess can be done with apical resection procedure. The main goal of apical resection is to prevent bacterial leakage from root canal by placing a hermetic root end filling following apical root resection. Purpose: to provide information about management of necrosis teeth with a large periapical abscess with apical resection procedure. Case: Female pasien, 23 years old, came to Conservative Dentistry Clinic, RSGM Universitas Airlangga. She is reffered from Ortodontic Clinic. Patient feels uncomfortable on her front teeth. Radiographic examination reveal radiolucency in the apical teeth 11 and 21 sized ±10mm.Case Management : Before surgery, root canal treatment on 11 and 21 was done. Preparation of the rooth canal using crown down pressureless technic and orthograde obturation. The next treatment was apical resection. Making semilu...
Background: the teeth are integrated part of facial aesthetic and are involved in a complex socia... more Background: the teeth are integrated part of facial aesthetic and are involved in a complex social, culture and psychological interaction. inapproprite endodontic and restoration treatment could cause some aesthetic problem. purpose: the aim of this article is to present a completecrown restoration and endodontic treatment to solve aesthetic problem in maxillary anterior teeth. case: in this case, a patient 28 years old man with a non vital pulp abd bad crown restoration of central incisor and labiovertion of lateral incisor maxillary teeth. case management: four incisor maxillary anterior teeth performing root canal treatment and lateral incisor having its inclination repaired using custom post. treatment was completed with porcelain fised to metal for all teeth. conclusion: complex aesthetic treatment using of bad anterior crown restoration included changing inclination. keywords: complex aesthetic, one visit endodontic, custom post, porcelain fused to metal
Background: Improper endodontic treatment may cause defect at bifurcation area. Tooth with defect... more Background: Improper endodontic treatment may cause defect at bifurcation area. Tooth with defect at the bifurcation area needs root canal treatment and endodontic surgery such as bicuspidization to repair the defect. Purpose: The aim of this case report is to report the treatment in mandibular right first molar with bifurcation perforation by bicuspidization surgery. Case: A 51 year-old female was referred to Conservative Dentistry Clinic Airlangga University, who suffered painful symptom in her mandibular right first molar. Intra oral examination showed an occlusal cavity with opened pulp, there is hole on the bottom of pulp chamber. There was no response to vitality test, percussion, palpation, and tooth mobility. Preoperative radiograph indicated the presence of radiolucency. Case management: The artificial wall and the closure of bifurcation perforation with glass ionomer cement were done. The biomechanical preparation was performed using crown down technique with ProTaper Next...
Exposed pulp will produce pro-inflammatory cytokines, which are the main target in inhibiting inf... more Exposed pulp will produce pro-inflammatory cytokines, which are the main target in inhibiting inflammation of the pulp. Early reparative dentin formation begins with the synthesis of extracellular matrix dentin such as type I collagen, which is believed to be the key of successful pulp capping treatment. Propolis is an alternative pulp capping material that is known to reduce inflammation in the exposed pulp. Calcium hydroxide (CH) is the gold standard for pulp capping material but many studies have shown some limitations on calcium hydroxide. This study was conducted to determine the expression of TNF-α and ALP after the application of a combination of calcium hydroxide and propolis extract as pulp capping material. The maxillary first molars of sixty rats (Wistar) were mechanically perforated. Teeth were divided into 3 groups containing 20 each. Group I as a control, group II treated with calcium hydroxide, group III treated with calcium hydroxide and propolis. The final restorati...
Inflammation in pulp tissue is caused by caries bacteria. Most bacteria found are Lactobacillus a... more Inflammation in pulp tissue is caused by caries bacteria. Most bacteria found are Lactobacillus acidophilus . Propolis is a sticky resin material that is derived from the bees and the surrounding plants, which are reported to have several biological effects including anti-bacterial and anti-inflammatory. This study will prove the effectiveness of propolis against proinflammatory cytokines on odontoblast like cells in human dental pulp. This study was performed on cultured odontoblast like cells in pulp. Cell culture was derived from the pulp tissue of human M3 teeth that had been extracted. Odontoblast pulp culture was divided in 3 groups, group 1 was cultured cells with propolis; group 2, cultured cells induced with Lactobacillus acidophilus ; group 3, cultured cells induced with inactive Lactobacillus acidophilus and exposed to 3μg/ml propolis. A measurement of TNF-α danTGF-β1 expression was done using the immunocytochemical technique to determine the effectiveness of propolis ext...
Background: Inflammation of the pulp can lead to elicit pain. Pain in inflammation is induced by ... more Background: Inflammation of the pulp can lead to elicit pain. Pain in inflammation is induced by the cyclooxygenase-2 enzyme (COX-2) which induces prostaglandin E2 (PGE2) resulting in pain. Pain in the pulp can be relieved by eugenol. In its application, eugenol is toxic to pulp fibroblasts. Due to the side effect, it is worth considering other biocompatible materials with minimal side effects, such as propolis. Flavonoids and phenolic acids that contained in propolis can inhibit COX-2. Therefore, an analysis outlined in the literature review is needed to examine the results of research related to the role of propolis as pulp pain relief by inhibiting COX-2 expression. Purpose: To analyze the role of propolis in pulp pain by inhibiting COX-2 expression. Reviews: Propolis extract that extracted by ethanol, water, and hydroalcohol has pain relief properties in the pulp by inhibiting COX-2 by directly binding to the COX-2 receptors and by reducing the production of proinflammatory cyto...
ODONTO : Dental Journal, 2017
Background: Lactobacillus acidophilus, Gram-positive bacterias that enterthe dentinal tissue duri... more Background: Lactobacillus acidophilus, Gram-positive bacterias that enterthe dentinal tissue during the carious process are suspected to influence the immune response in human dental pulp. Odontoblasts situated at the pulp dentin interface are the frst cells encountered by these bacteria and have an important role in this response. Lipoteichoic acid (LTA), a wall component of Gram-positive bacteria, triggered the activation of the odontoblasts. LTA upregulated the expression of its own receptor TLR2, as well as the production of proinflammatory cytokineTNFα. Propolis is a resinous material that holds a great potential as an antiinflammatory agent. Present studies have shown that propolis has a reduction effect towards the proinflammatory cytokines expression and favor pulp healing.Purpose: To reveal the moleculer mechanism of propolis stimulation onodontoblast like cells, induced by inactive Lactobacillus acidophilus.Methods: This review was presented in odontoblasts like cells culture i...
Conservative Dentistry Journal, 2020
Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold s... more Background: Pulp inflammation can be treated with pulp capping by calcium hydroxide as the gold standard. Pulp capping by calcium hydroxide could causing tissue necrosis followed by inflammation. Calcium hydroxide combined with propolis which have anti-inflammatory effects through inhibition of the Nf-kB pathway and pro-inflammatory cytokines. That process will inhibit the increase of vascular permeability and neutrophil chemotaxis. Inflammatory process can accelerate by controlling the amount of neutrophil cells as a acute phase marker, so the healing process occurs more quickly. Therefore it is necessary to study the amount of neutrophil cells in the pulp perforation of Wistar rats after applicated by combination of calcium hydroxide-propolis. Purpose: To analyze the difference amount of neutrophil cells on the Wistar rats pulp perforation after applicated by calcium hydroxide-propolis combination compared with calcium hydroxide-aquadest Method: M1 tooth perforated for all groups ...
Acta Medica Philippina, 2019
Orthostatic hypotension occurs when cardiovascular adaptive mechanisms fail to compensate the red... more Orthostatic hypotension occurs when cardiovascular adaptive mechanisms fail to compensate the reduction in venous return that normally occurs on the upright position. A patient with orthostatic hypotension can be a challenging case for a dentist. A 78-year-old male came with idiopatic orthostatic hypotension and felt uncomfortable with his old crown restoration and discoloration on his upper teeth. Management of orthostatic hypotension can be handled by manipulating the dental chair interval movement position.
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Papers by Ira Widjiastuti