MYH9 has been identified as an indispensable cellular protein for porcine reproductive and respir... more MYH9 has been identified as an indispensable cellular protein for porcine reproductive and respiratory syndrome virus (PRRSV) entry into permissive cells using the monoclonal anti-idiotypic antibody (Mab2-5G2) recognizing an antibody that specifically interacts with PRRSV glycoprotein 5 (GP5). More recently, we found that Mab2-5G2 interacted with the MYH9 C-terminal domain, designated PRA, which is required for PRRSV internalization. In this study, we demonstrate that blocking of MYH9 with Mab2-5G2 significantly diminished PRRSV internalization by porcine alveolar macrophage (PAM) via interruption of direct interaction between GP5 and MYH9, and thus remarkably inhibited subsequent infection of PAMs by PRRSV-2 isolates. Moreover, the three-dimensional structure of the Mab2-5G2 Fab-PRA complex determined via homology modeling predicted potential docking sites required for PRRSV internalization. Further analysis of Mab2-5G2-binding sites within PRA highlighted that the amino acids E167...
Celangulin V (CV) is a compound isolated from Celastrus angulatus Max that has a toxic activity a... more Celangulin V (CV) is a compound isolated from Celastrus angulatus Max that has a toxic activity against agricultural insect pests. CV can bind to subunits a, H, and B of the vacuolar ATPase (V-ATPase) in the midgut epithelial cells of insects. However, the mechanism of action of CV is still unclear. In this study, the soluble complex of the V-ATPase A subunit mutant TSCA which avoids the feedback inhibition by the hydrolysate ADP and V-ATPase B subunit were obtained and then purified using affinity chromatography. The H+K+-ATPase activity of the complex and the inhibitory activity of CV on ATP hydrolysis were determined. The results suggest that CV inhibits the ATP hydrolysis, resulting in an insecticidal effect. Additionally, the homology modeling of the AB complex and molecular docking results indicate that CV can competitively bind to the AB complex at the ATP binding site, which inhibits ATP hydrolysis. These findings suggest that the AB subunits complex is one of the potential ...
Japanese quail (Coturnix japonica), a recently domesticated poultry species, is important not onl... more Japanese quail (Coturnix japonica), a recently domesticated poultry species, is important not only as an agricultural product, but also as a model bird species for genetic research. However, most of the biological questions concerning genomics, phylogenetics and genetics of some important economic traits have not been answered. It is thus necessary to complete a high-quality genome sequence as well as a series of comparative genomics, evolution and functional studies. Here, we present a quail genome assembly spanning 1.04Gb with 86.63% of sequences anchored to 30 chromosomes (28 autosomes and two sex chromosomes Z/W). Our genomic data has resolved the long-term debate of phylogeny among Perdicinae (Japanese quail), Meleagridinae (turkey) and Phasianinae (chicken). Comparative genomics and functional genomic data found that four candidate genes involved in early maturation had experienced positive selection, and one of them encodes follicle stimulating hormone beta (FSHβ), which is c...
International journal of molecular sciences, Jan 8, 2018
miR-25, a member of the miR-106b-25 cluster, has been reported as playing an important role in ma... more miR-25, a member of the miR-106b-25 cluster, has been reported as playing an important role in many biological processes by numerous studies, while the role of miR-25 in metabolism and its transcriptional regulation mechanism remain unclear. In this study, gain-of-function and loss-of-function assays demonstrated that miR-25-3p positively regulated the metabolism of C2C12 cells by attenuating phosphoinositide 3-kinase () gene expression and triglyceride (TG) content, and enhancing the content of adenosine triphosphate (ATP) and reactive oxygen species (ROS). Furthermore, the results from bioinformatics analysis, dual luciferase assay, site-directed mutagenesis, qRT-PCR, and Western blotting demonstrated that miR-25-3p directly targeted the AKT serine/threonine kinase 1 () 3' untranslated region (3'UTR). The core promoter of miR-25-3p was identified, and the transcription factor activator protein-2α (AP-2α) significantly increased the expression of mature miR-25-3p by binding...
Analytical and bioanalytical chemistry, Jan 9, 2017
A simple sample preparation method requiring minimal organic solvents is proposed for the determi... more A simple sample preparation method requiring minimal organic solvents is proposed for the determination of the total phthalate content in cosmetics by high-performance liquid chromatography-tandem mass spectrometry. The hydrolysis of phthalates and purification of interfering substances were performed in a three-phase system that included an upper n-hexane phase, a middle ethanol phase, and a lower aqueous alkali solution. This three-phase system utilized an incremental purification strategy. The apolar ingredients were extracted with n-hexane, the polar pigments accumulated in the ethanol phase, and the hydrolysis product, phthalic acid, remained in the hydrolysate. Under the optimized conditions, the correlation coefficients (r) for the calibration curves were 0.998-0.999 in the range 0.60-12 mol L-1. The limit of detection was 5.1 μmol kg-1, and the limit of quantification was 9.2 μmol kg-1. The recoveries varied from 84 to 97% with RSDs equal to or lower than 11%. The intra-day ...
Circular RNAs (circRNAs) are a new class of non-coding RNAs in animals and are a novel target of ... more Circular RNAs (circRNAs) are a new class of non-coding RNAs in animals and are a novel target of non-coding RNA (ncRNA) regulation. The mechanism and function of circRNAs have been reported in some species and tissues. However, there is little available information on the functions of circRNAs in the goat reproductive system. In the present study, we deeply sequenced and analyzed circRNAs through bioinformatics to reveal the expression profiles, and predicted 13,950 circRNAs in the pre-ovulatory ovarian follicles of goats for the first time. Thirty-seven circRNAs were differentially expressed in the Boer goat compared with the Macheng black goat. The chi_circ_0008219 was involved in a vast circRNA-miRNA-mRNA co-expression network. Via a luciferase activity assay, chi_circ_0008219 is observed to sponge to 3 ovarian follicle-related miRNAs. These findings demonstrate that circRNAs have potential effects in the ovarian follicles of ewes and may represent a promising new research field ...
Non-structural protein 7 (NSP7), which can be further cleaved into NSP7α and NSP7β, is one of the... more Non-structural protein 7 (NSP7), which can be further cleaved into NSP7α and NSP7β, is one of the most conserved proteins of porcine reproductive and respiratory syndrome virus (PRRSV). NSP7 plays a role in provoking the humoral immune system in PRRSV-infected swine, but its structure and function are still not fully understood. Here, we analyzed the expression of NSP7, NSP7α, and NSP7β in PRRSV-infected MARC-145 cells. The solution structure of NSP7α was determined by using nuclear magnetic resonance (NMR). Although the structure provided little clue to its function, based on the structure of NSP7α, we predicted and further identified some key amino acids on NSP7α for the interaction of NSP7α with NSP9, the RNA dependent RNA polymerase of PRRSV. This study provided some new insights into the structure and function of PRRSV NSP7.
The current routes to couple dispersive liquid-liquid microextraction with capillary electrophore... more The current routes to couple dispersive liquid-liquid microextraction with capillary electrophoresis are the evaporation of water immiscible extractants and the back-extraction of analytes. In this study, a new methodology for this combination using water-in-oil microemulsion electrokinetic chromatography coupled with normal stacking mode on-line sample concentration was developed to analyze chlorophenols in water samples. The analytes were extracted with tributyl phosphate and the extractant dilution (3 ×) was directly injected into an electrophoresis buffer (7.7 cm) containing 5% sodium dodecyl sulfate, 78% 1-butanol, 2% 1-heptane and 15% sodium acetate solution (pH 8.0). This proposed method is very simple and convenient compared to the conventional procedures. The key parameters affecting separation and concentration were systematically optimized. Under the optimized conditions, dispersive liquid-liquid microextraction contributed an enrichment factor of 45-50, and the overall s...
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2016
A new clinic conception, healthy cosmetic management (HCM), was innovated introduced into diagnos... more A new clinic conception, healthy cosmetic management (HCM), was innovated introduced into diagnose and treatment of patients with high caries risk. The priority of HCM should be given to aesthetic restoration of teeth, taking consideration of the conserving of tissue and pulp of teeth and the balance between health and aesthetics, which may reach to the goal of the individual clinic treatment. HCM includes five steps: 1) caries risk assessment; 2) detection of initial caries; 3) digital aesthetic design; 4) minimally invasive therapy; 5) behavior management of caries and doctor-patient communication pattern. In this article, HCM is introduced into two aspects: process management and clinical protocols, followed by the combination of the clinical practice.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine rep... more Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome, a destructive disease of swine. PRRSV has a single strand positive-sense RNA genome which contains at least ten open reading frames, of these, ORF1a and ORF1b encode polyproteins pp1a and pp1ab. Subsequently, pp1a is cleaved into ten nonstructural proteins, including nonstructural protein 7α and 7β (nsp7α and 7β), the internal cleavage products of a conserved nonstructural protein nsp7. Nsp7 plays a role in provoking the humoral immune system into producing anti-nsp7 antibodies which can be highly and persistently expressed in PRRSV-infected swine. However, the functions of nsp7α and 7β remain unknown. Western blot and radioimmunoprecipitation analysis of the two proteins showed that only cleaved nsp7α was detectable and cleaved nsp7β was not detected in the infected cells. Here, we reported the (1)H, (13)C and (15)N resonance assignment of nsp7α from ...
Advanced materials (Deerfield Beach, Fla.), Jan 28, 2015
The combination of water-based titanate nanosheets dispersion and silk fibroin solution allow the... more The combination of water-based titanate nanosheets dispersion and silk fibroin solution allow the realization of a versatile nanocomposite. Different fabrication techniques can be easily applied on these nanocomposites to manipulate the end form of these materials on the micro- and nanoscale. Easy tunability of the refractive index from n = 1.55 up to n = 1.97 is achieved, making it attractive for flexible, biopolymer-based optical devices.
The recent introduction of transient, bioresorbable electronics into the field of electronic devi... more The recent introduction of transient, bioresorbable electronics into the field of electronic device design offers promise for the areas of medical implants and environmental monitors, where programmed loss of function and environmental resorption are advantageous characteristics. Materials challenges remain, however, in protecting the labile device components from degradation at faster than desirable rates. Here we introduce an indirect passivation strategy for transient electronic devices that consists of encapsulation in multiple air pockets fabricated from silk fibroin. This approach is investigated through the properties of silk as a diffusional barrier to water penetration, coupled with the degradation of magnesium-based devices in humid air. Finally, silk pockets are demonstrated to be useful for controlled modulation of device lifetime. This approach may provide additional future opportunities for silk utility due to the low immunogenicity of the material and its ability to s...
Dens evaginatus (DE) is a dental anomaly that occurs as an accessory tubercle on the occlusal or ... more Dens evaginatus (DE) is a dental anomaly that occurs as an accessory tubercle on the occlusal or lingual surface of a tooth. The authors provide a literature review and report a rare case in which DE occurs on multiple mandibular premolars and maxillary molars. The patient is a 26-year-old Chinese woman, with a chief complaint of gingival bleeding. DE affecting teeth 17, 27, 35, and 45 was found during clinical examination. For treatment of the patient, we reduced the opposing occluding teeth, while undertaking progressive grinding of the tubercles for six months. We followed-up for a year. This suggests the importance of examining for multiple DE during clinical practice.
Recent developments in fl exible/strechable electronics [ 1-3 ] and optoelectronics [ 4-6 ] demon... more Recent developments in fl exible/strechable electronics [ 1-3 ] and optoelectronics [ 4-6 ] demonstrate that high-performance semiconductor device functionality can be achieved in forms that enable intimate, conformal contacts to static or timedynamic curvilinear surfaces, [ 7 , 8 ] with promising applications in advanced biomedical devices and other areas that cannot
An essential issue in future climate treaties is how to bring developing countries on board. 38 c... more An essential issue in future climate treaties is how to bring developing countries on board. 38 countries have ratified the Kyoto Protocol and committed to greenhouse gas emission reductions. But these countries cover less than a third of global CO 2-emissions (IEA, 2007) and the share is falling over Resource and Energy Economics 31 (2009) 139-160
ABSTRACT The inclination (i) and position angle (PA) of the Whirlpool galaxy (M51) are critical t... more ABSTRACT The inclination (i) and position angle (PA) of the Whirlpool galaxy (M51) are critical to modeling and interpreting observations. Here we make improved estimates of these parameters by fitting logarithmic spirals to the main arms. From separate fits to each major arm, we obtain i = 20. Degree-Sign 3 {+-} 2. Degree-Sign 8 and PA = 12. Degree-Sign 0 {+-} 2. Degree-Sign 5. We then use Poisson's equation for the logarithmic perturbation of the density to estimate the mean vertical scale height (H) of M51 to be 95-178 pc.
This article was published in an Elsevier journal. The attached copy is furnished to the author f... more This article was published in an Elsevier journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the author's institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright
☆ Thanks to Zhai Fan for help in building the scenarios, and to Taran Faehn, Hans Martin Seip and... more ☆ Thanks to Zhai Fan for help in building the scenarios, and to Taran Faehn, Hans Martin Seip and a referee for the thoughtful comments.
MYH9 has been identified as an indispensable cellular protein for porcine reproductive and respir... more MYH9 has been identified as an indispensable cellular protein for porcine reproductive and respiratory syndrome virus (PRRSV) entry into permissive cells using the monoclonal anti-idiotypic antibody (Mab2-5G2) recognizing an antibody that specifically interacts with PRRSV glycoprotein 5 (GP5). More recently, we found that Mab2-5G2 interacted with the MYH9 C-terminal domain, designated PRA, which is required for PRRSV internalization. In this study, we demonstrate that blocking of MYH9 with Mab2-5G2 significantly diminished PRRSV internalization by porcine alveolar macrophage (PAM) via interruption of direct interaction between GP5 and MYH9, and thus remarkably inhibited subsequent infection of PAMs by PRRSV-2 isolates. Moreover, the three-dimensional structure of the Mab2-5G2 Fab-PRA complex determined via homology modeling predicted potential docking sites required for PRRSV internalization. Further analysis of Mab2-5G2-binding sites within PRA highlighted that the amino acids E167...
Celangulin V (CV) is a compound isolated from Celastrus angulatus Max that has a toxic activity a... more Celangulin V (CV) is a compound isolated from Celastrus angulatus Max that has a toxic activity against agricultural insect pests. CV can bind to subunits a, H, and B of the vacuolar ATPase (V-ATPase) in the midgut epithelial cells of insects. However, the mechanism of action of CV is still unclear. In this study, the soluble complex of the V-ATPase A subunit mutant TSCA which avoids the feedback inhibition by the hydrolysate ADP and V-ATPase B subunit were obtained and then purified using affinity chromatography. The H+K+-ATPase activity of the complex and the inhibitory activity of CV on ATP hydrolysis were determined. The results suggest that CV inhibits the ATP hydrolysis, resulting in an insecticidal effect. Additionally, the homology modeling of the AB complex and molecular docking results indicate that CV can competitively bind to the AB complex at the ATP binding site, which inhibits ATP hydrolysis. These findings suggest that the AB subunits complex is one of the potential ...
Japanese quail (Coturnix japonica), a recently domesticated poultry species, is important not onl... more Japanese quail (Coturnix japonica), a recently domesticated poultry species, is important not only as an agricultural product, but also as a model bird species for genetic research. However, most of the biological questions concerning genomics, phylogenetics and genetics of some important economic traits have not been answered. It is thus necessary to complete a high-quality genome sequence as well as a series of comparative genomics, evolution and functional studies. Here, we present a quail genome assembly spanning 1.04Gb with 86.63% of sequences anchored to 30 chromosomes (28 autosomes and two sex chromosomes Z/W). Our genomic data has resolved the long-term debate of phylogeny among Perdicinae (Japanese quail), Meleagridinae (turkey) and Phasianinae (chicken). Comparative genomics and functional genomic data found that four candidate genes involved in early maturation had experienced positive selection, and one of them encodes follicle stimulating hormone beta (FSHβ), which is c...
International journal of molecular sciences, Jan 8, 2018
miR-25, a member of the miR-106b-25 cluster, has been reported as playing an important role in ma... more miR-25, a member of the miR-106b-25 cluster, has been reported as playing an important role in many biological processes by numerous studies, while the role of miR-25 in metabolism and its transcriptional regulation mechanism remain unclear. In this study, gain-of-function and loss-of-function assays demonstrated that miR-25-3p positively regulated the metabolism of C2C12 cells by attenuating phosphoinositide 3-kinase () gene expression and triglyceride (TG) content, and enhancing the content of adenosine triphosphate (ATP) and reactive oxygen species (ROS). Furthermore, the results from bioinformatics analysis, dual luciferase assay, site-directed mutagenesis, qRT-PCR, and Western blotting demonstrated that miR-25-3p directly targeted the AKT serine/threonine kinase 1 () 3' untranslated region (3'UTR). The core promoter of miR-25-3p was identified, and the transcription factor activator protein-2α (AP-2α) significantly increased the expression of mature miR-25-3p by binding...
Analytical and bioanalytical chemistry, Jan 9, 2017
A simple sample preparation method requiring minimal organic solvents is proposed for the determi... more A simple sample preparation method requiring minimal organic solvents is proposed for the determination of the total phthalate content in cosmetics by high-performance liquid chromatography-tandem mass spectrometry. The hydrolysis of phthalates and purification of interfering substances were performed in a three-phase system that included an upper n-hexane phase, a middle ethanol phase, and a lower aqueous alkali solution. This three-phase system utilized an incremental purification strategy. The apolar ingredients were extracted with n-hexane, the polar pigments accumulated in the ethanol phase, and the hydrolysis product, phthalic acid, remained in the hydrolysate. Under the optimized conditions, the correlation coefficients (r) for the calibration curves were 0.998-0.999 in the range 0.60-12 mol L-1. The limit of detection was 5.1 μmol kg-1, and the limit of quantification was 9.2 μmol kg-1. The recoveries varied from 84 to 97% with RSDs equal to or lower than 11%. The intra-day ...
Circular RNAs (circRNAs) are a new class of non-coding RNAs in animals and are a novel target of ... more Circular RNAs (circRNAs) are a new class of non-coding RNAs in animals and are a novel target of non-coding RNA (ncRNA) regulation. The mechanism and function of circRNAs have been reported in some species and tissues. However, there is little available information on the functions of circRNAs in the goat reproductive system. In the present study, we deeply sequenced and analyzed circRNAs through bioinformatics to reveal the expression profiles, and predicted 13,950 circRNAs in the pre-ovulatory ovarian follicles of goats for the first time. Thirty-seven circRNAs were differentially expressed in the Boer goat compared with the Macheng black goat. The chi_circ_0008219 was involved in a vast circRNA-miRNA-mRNA co-expression network. Via a luciferase activity assay, chi_circ_0008219 is observed to sponge to 3 ovarian follicle-related miRNAs. These findings demonstrate that circRNAs have potential effects in the ovarian follicles of ewes and may represent a promising new research field ...
Non-structural protein 7 (NSP7), which can be further cleaved into NSP7α and NSP7β, is one of the... more Non-structural protein 7 (NSP7), which can be further cleaved into NSP7α and NSP7β, is one of the most conserved proteins of porcine reproductive and respiratory syndrome virus (PRRSV). NSP7 plays a role in provoking the humoral immune system in PRRSV-infected swine, but its structure and function are still not fully understood. Here, we analyzed the expression of NSP7, NSP7α, and NSP7β in PRRSV-infected MARC-145 cells. The solution structure of NSP7α was determined by using nuclear magnetic resonance (NMR). Although the structure provided little clue to its function, based on the structure of NSP7α, we predicted and further identified some key amino acids on NSP7α for the interaction of NSP7α with NSP9, the RNA dependent RNA polymerase of PRRSV. This study provided some new insights into the structure and function of PRRSV NSP7.
The current routes to couple dispersive liquid-liquid microextraction with capillary electrophore... more The current routes to couple dispersive liquid-liquid microextraction with capillary electrophoresis are the evaporation of water immiscible extractants and the back-extraction of analytes. In this study, a new methodology for this combination using water-in-oil microemulsion electrokinetic chromatography coupled with normal stacking mode on-line sample concentration was developed to analyze chlorophenols in water samples. The analytes were extracted with tributyl phosphate and the extractant dilution (3 ×) was directly injected into an electrophoresis buffer (7.7 cm) containing 5% sodium dodecyl sulfate, 78% 1-butanol, 2% 1-heptane and 15% sodium acetate solution (pH 8.0). This proposed method is very simple and convenient compared to the conventional procedures. The key parameters affecting separation and concentration were systematically optimized. Under the optimized conditions, dispersive liquid-liquid microextraction contributed an enrichment factor of 45-50, and the overall s...
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2016
A new clinic conception, healthy cosmetic management (HCM), was innovated introduced into diagnos... more A new clinic conception, healthy cosmetic management (HCM), was innovated introduced into diagnose and treatment of patients with high caries risk. The priority of HCM should be given to aesthetic restoration of teeth, taking consideration of the conserving of tissue and pulp of teeth and the balance between health and aesthetics, which may reach to the goal of the individual clinic treatment. HCM includes five steps: 1) caries risk assessment; 2) detection of initial caries; 3) digital aesthetic design; 4) minimally invasive therapy; 5) behavior management of caries and doctor-patient communication pattern. In this article, HCM is introduced into two aspects: process management and clinical protocols, followed by the combination of the clinical practice.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine rep... more Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of porcine reproductive and respiratory syndrome, a destructive disease of swine. PRRSV has a single strand positive-sense RNA genome which contains at least ten open reading frames, of these, ORF1a and ORF1b encode polyproteins pp1a and pp1ab. Subsequently, pp1a is cleaved into ten nonstructural proteins, including nonstructural protein 7α and 7β (nsp7α and 7β), the internal cleavage products of a conserved nonstructural protein nsp7. Nsp7 plays a role in provoking the humoral immune system into producing anti-nsp7 antibodies which can be highly and persistently expressed in PRRSV-infected swine. However, the functions of nsp7α and 7β remain unknown. Western blot and radioimmunoprecipitation analysis of the two proteins showed that only cleaved nsp7α was detectable and cleaved nsp7β was not detected in the infected cells. Here, we reported the (1)H, (13)C and (15)N resonance assignment of nsp7α from ...
Advanced materials (Deerfield Beach, Fla.), Jan 28, 2015
The combination of water-based titanate nanosheets dispersion and silk fibroin solution allow the... more The combination of water-based titanate nanosheets dispersion and silk fibroin solution allow the realization of a versatile nanocomposite. Different fabrication techniques can be easily applied on these nanocomposites to manipulate the end form of these materials on the micro- and nanoscale. Easy tunability of the refractive index from n = 1.55 up to n = 1.97 is achieved, making it attractive for flexible, biopolymer-based optical devices.
The recent introduction of transient, bioresorbable electronics into the field of electronic devi... more The recent introduction of transient, bioresorbable electronics into the field of electronic device design offers promise for the areas of medical implants and environmental monitors, where programmed loss of function and environmental resorption are advantageous characteristics. Materials challenges remain, however, in protecting the labile device components from degradation at faster than desirable rates. Here we introduce an indirect passivation strategy for transient electronic devices that consists of encapsulation in multiple air pockets fabricated from silk fibroin. This approach is investigated through the properties of silk as a diffusional barrier to water penetration, coupled with the degradation of magnesium-based devices in humid air. Finally, silk pockets are demonstrated to be useful for controlled modulation of device lifetime. This approach may provide additional future opportunities for silk utility due to the low immunogenicity of the material and its ability to s...
Dens evaginatus (DE) is a dental anomaly that occurs as an accessory tubercle on the occlusal or ... more Dens evaginatus (DE) is a dental anomaly that occurs as an accessory tubercle on the occlusal or lingual surface of a tooth. The authors provide a literature review and report a rare case in which DE occurs on multiple mandibular premolars and maxillary molars. The patient is a 26-year-old Chinese woman, with a chief complaint of gingival bleeding. DE affecting teeth 17, 27, 35, and 45 was found during clinical examination. For treatment of the patient, we reduced the opposing occluding teeth, while undertaking progressive grinding of the tubercles for six months. We followed-up for a year. This suggests the importance of examining for multiple DE during clinical practice.
Recent developments in fl exible/strechable electronics [ 1-3 ] and optoelectronics [ 4-6 ] demon... more Recent developments in fl exible/strechable electronics [ 1-3 ] and optoelectronics [ 4-6 ] demonstrate that high-performance semiconductor device functionality can be achieved in forms that enable intimate, conformal contacts to static or timedynamic curvilinear surfaces, [ 7 , 8 ] with promising applications in advanced biomedical devices and other areas that cannot
An essential issue in future climate treaties is how to bring developing countries on board. 38 c... more An essential issue in future climate treaties is how to bring developing countries on board. 38 countries have ratified the Kyoto Protocol and committed to greenhouse gas emission reductions. But these countries cover less than a third of global CO 2-emissions (IEA, 2007) and the share is falling over Resource and Energy Economics 31 (2009) 139-160
ABSTRACT The inclination (i) and position angle (PA) of the Whirlpool galaxy (M51) are critical t... more ABSTRACT The inclination (i) and position angle (PA) of the Whirlpool galaxy (M51) are critical to modeling and interpreting observations. Here we make improved estimates of these parameters by fitting logarithmic spirals to the main arms. From separate fits to each major arm, we obtain i = 20. Degree-Sign 3 {+-} 2. Degree-Sign 8 and PA = 12. Degree-Sign 0 {+-} 2. Degree-Sign 5. We then use Poisson's equation for the logarithmic perturbation of the density to estimate the mean vertical scale height (H) of M51 to be 95-178 pc.
This article was published in an Elsevier journal. The attached copy is furnished to the author f... more This article was published in an Elsevier journal. The attached copy is furnished to the author for non-commercial research and education use, including for instruction at the author's institution, sharing with colleagues and providing to institution administration. Other uses, including reproduction and distribution, or selling or licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or institutional repository. Authors requiring further information regarding Elsevier's archiving and manuscript policies are encouraged to visit: http://www.elsevier.com/copyright
☆ Thanks to Zhai Fan for help in building the scenarios, and to Taran Faehn, Hans Martin Seip and... more ☆ Thanks to Zhai Fan for help in building the scenarios, and to Taran Faehn, Hans Martin Seip and a referee for the thoughtful comments.
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