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Hassan Elfadaly

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Assistant Research Professor, medical educator (veterinary division).Zoonotic Diseases Department National Research Center, Giza.

Master Degree of Vet. Sci. (zoonosis), Faculty of Vet. Med., Cairo Univ.,, 2002, Cairo University. Epidemiological studies on toxoplasmosis in at risk sheep, goats and women.

Doctor of Philosophy in Veterinary Medicine Ph. D. Degree of Vet. Sci., (zoonosis), Faculty of Vet. Med. Cairo Univ., 2007,.Advanced epidemiological studies on toxoplasmosis in animals and man.
Supervisors: Achievements include researches in zoonotic diseases, public health and food inspection aspects. Education
Phone: 01100998564
Address: Depatment of Zoonotic Diseases, Veterinary Research Division, National, Research Center, P.O. Box 12622 El-Tahrir
Street, Dokki, Giza, Egypt.

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Papers by Hassan Elfadaly

Elicitation of Subjective Probability Distributions

To incorporate expert opinion into a Bayesian analysis, it must be quantified as a prior distribu... more To incorporate expert opinion into a Bayesian analysis, it must be quantified as a prior distribution through an elicitation process that asks the expert meaningful questions whose answers determine this distribution. The aim of this thesis is to fill some gaps in the available techniques for eliciting prior distributions for Generalized Linear Models (GLMs) and multinomial models. A general method for quantifying opinion about GLMs was developed in Garthwaite and Al-Awadhi (2006). They model the relationship between each continuous predictor and the dependant variable as a piecewise-linear function with a regression coefficient at each of its dividing points. However, coefficients were assumed a priori independent if associated with different predictors. We relax this simplifying assumption and propose three new methods for eliciting positive-definite variance-covariance matrices of a multivariate normal prior distribution. In addition, we extend the method of Garthwaite and Dickey...

In vitro Effects of Some Bee Products on T. gondii Tachyzoites

Toxoplasma gondii, is ideal opportunistic zoonotic protozoan, exceeds neurological and congenital... more Toxoplasma gondii, is ideal opportunistic zoonotic protozoan, exceeds neurological and congenital impact sequence to flare up of latent toxoplasmosis. It is intracellular pathogen and is so difficult to treat, it requires higher doses of intracellular antimicrobial drugs which reflect repulsive side actions. So, the aim of a current study is focused to evaluate the effect of some bee products (propolis, honey and bee venom) with special emphasis to sulfa-dugs as anti-Toxoplasma activity. The results revealed that all investigated bee products showed destructive on viable tachyzoites which promoted directly with the different dilutions of the bee products, in particular; 100, 50, 25 and 10 respectively, at chilling temperature (4°C) and corresponding to LD & LD. Bee venom proofed the faster and powerful in vitro anti-tachyzoites action with all dilutions, 50 100 exceeding even the control sulfonamides. 5, 6, 7 and 8 respectively while diluted propolis were effective to enhance the persisting viable tachyzoites course. Where 7% dilution of propolis symbolizes the maximum optimal value, successfully increases the average percent of LD &LD viable tachyzoites longer time a live 50 100 up to 172.9 hours post exposure to propolis, exceeding about three days over the normal control saline solution. In conclusion: the results revealed that bee products are promising for development of potent and novel antitoxoplasma drug.

Serological and molecular diagnosis of T . gondii in complicated pregnant Egyptian women

In pregnant women, primary Toxoplasma infection or reactivated chronic infection can lead to tran... more In pregnant women, primary Toxoplasma infection or reactivated chronic infection can lead to transplacental transmission with potential risk of congenital toxoplasmosis depending on the stage of pregnancy. Blood and matched placental and /or fetal tissue samples were collected from one hundred and six Egyptian women from the labor ward of Beni-Suef General Hospital and Kasr El-Eini HospitalEgypt, which are suffering from complicated pregnancy. The study includes G1, G2, G3 and G4 are symbolized the abortion corresponding to the 1st, 2nd and 3nd trimesters with congenital anomalies. All women were assayed serologically by IgM and IgG ELISA and molecular identification by PCR for confirming T.gondii infection. The sero-positive total percentage was 42.4%, 35% and 33% using ELISA, Latex agglutination and PCR respectively.It was concluded that all T.gondii sero-positive women were harboring DNA or compatible placental cysts; denote that serological assay was not accurate alone for confi...

Advanced epidemiological studies on toxoplasmosis in animals and man

Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular To... more Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular Toxoplasma gondii protozoan capable of infecting all types of nucleated mammalian host cells. The disease is of economic veterinary importance and of public health hazard in human side. In the present study, antigens were successfully prepared from RH strain tachyzoites and were used for sero-diagnosis of random samples of both human& buffaloes by ELISA and SFDT. Where, the percent of sero-positive was [56.2&50] and [14.6&10.1] respectively. These results were compared with the commercial LAT that gave uncorrelated results and judged as unsuitable test for diagnostic purposes. New techniques were used for developing the performance of maintenance and preservation of RH strain tachyzoites. A modified method for long term acute stage in mice was used for the first time and succeeds to harboring the T. gondii protozoan up to 12 DPI. Also, it was proven that the liquid nitrogen preservation is...

THERAPEUTIC EFFECT OF NITAZOXANIDE AND ZINGIBER OFFICINALE IN TREATMENT OF ACUTE TOXOPLASMOSIS GONDII USING MURINE EXPERIMENTAL MODEL By

Toxoplasmosis is a cosmopolitan parasitic disease that impacts enormous population sector. In thi... more Toxoplasmosis is a cosmopolitan parasitic disease that impacts enormous population sector. In this work we investigated the antitoxoplamic therapeutic effect of one synthetic drug; nitazoxanide (NTZ) and another agent of plant origin; Zingiber officinale (ginger) as adjuvant to the traditional antotoxoplamic drugs (pyrimethamine & sulphadiazine) on experimental murine toxoplasmosis (RH virulent strain). Animals were classified into 7 groups; GI: Non-infected, nontreated (normal control), GII: Infected non -treated mice (infected control), GIII: Infected & treated by a combination of pyrimethamine & sulphadiazine (drug control), Group IV: Infected & treated by Zingiber officinale GV: Infected & treated by nitazoxanide, GVI: Infected & treated by combination of Zingiber officinale and pyrimethamine & sulphadiazine, Group VII: Infected & treated by combination of Zingiber officinale & nitazoxanide. For assessment of the therapeutic effect of various drug regimens on acute toxoplasmosis...

Zoonotic Hazards of Campylobacteriosis in some areas in Egypt

A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of pat... more A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of patient with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt. [Barakat A. M. A. Mona M. Sobhy; E...

Zoonotic concern of Campylobacter jejuni in raw and ready-to-eat barbequechickens along with Egyptian handlers and consumers via molecular andimmunofluorescent characterization

Raw and under cooked barbeque chickenswhich harboring pathogens of public health and zoonotic imp... more Raw and under cooked barbeque chickenswhich harboring pathogens of public health and zoonotic impact concerning consumers and handler employees. Campylobacter jejuni considers one of the most prevalent chickenborne gastroenteric bacteria. This study spots light on this concept to indemnify zoonotic hazard of C. jejuni by molecular characterization and indirect fluorescent of Egyptian isolates from both chickens and human in contact. From various Egyptian governorates and clinics a total of 588 chicken visceral contents, eviscerated raw and barbeque chickens were collected from different restaurants. Plus, 96 samples from both symptomatic consumers with history of chickens poisoning and chicken handler. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hipO gene. The positive results were detected by IFT expressed by green fluorescence s...

Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinella spiralis

Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from ... more Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from toxoplasmosis or trichinellosis may present with the same clinical signs. A total of 166 serum samples from suspected cases of toxoplasmosis were collected (Egypt). ELISA results showed that 54.21% of serum samples were positive for Toxoplasma and 90% of the positive anti-Toxoplasma patient sera were also positive for T. spiralis larval antigen. SDS profiles of the two parasites antigens displayed common bands at 130.95, 116.6, 66.2 and 55.38 KDa. Also, the immunoblot profiles of T. gondii and T. spiralis antigens blotted with ELISA positive human sera showed common immunoreactive bands at 130.95., 113.60, 55.69 and 28.42 KDa. We can conclude that there is a strong cross reactivity between the two parasites and some of these proteins could be useful in developing vaccines or drugs for controlling the diseases caused by the two parasites. Also, we may suggest that 94.20, 36.34 and 33.05 KDa and 64.33 and 44.57 KDa are specific bands for T. gondii and T. spiralis, respectively.

Ecological and Pathological Study of T. gondii Egyptian Rat Isolates Reference to Biological & Genetic Typescripts

Toxoplasma gondii is zoonotic opportunistic protozoan of public health impact. It has usual clona... more Toxoplasma gondii is zoonotic opportunistic protozoan of public health impact. It has usual clonal population of three virulent human and animal types: I, II, and III. The commercial rats are frequently harboring the dormant T. gondii tissue cysts in their tissues. So, rats consider the major prey for outdoor cats that regularly stimulating it to shed and contaminate the environment with oocysts infective stage which maximizes human and animals' toxoplasmosis. The aim of the present study is to identify the biological and molecular typescripts of naturally infected commercial rats along with serological, mice viability and histo-pathological assays for clarifying to how extent the indirect zoonotic bio-hazards sequence to rats harboring T. gondiivirulent types. A total number of 278 commercial rats were recognized as R. norvegicus (n=74), Rattus rattus rattus(n=108)and Rattus rattus frugivorus (n=96), were collected from different rural and urban sites from Cairo and Giza govern...

Incidence of zoonotic Campylobacter jejuni in fast meal meat, grill chickens and symptomatic Egyptians

African Journal of Microbiology Research, 2016

Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains ... more Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains of C. jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and produces diffuse bloody edematous exudative enteritis. The common routes of transmission are fecal-oral, person-to-person and the eating of raw or undercooked chickens or meat. This study recognizes the incidence of zoonotic C. jejuni in under cooked chickens and meat meals along with persons in contact. We examined 640 grilled chickens and 733 fast meat meals, plus 93 of symptomatic consumers and handlers were collected from five Egyptian governorates (Fayuom, Cairo, Qaluobia, Bin-suef and Assuit) from different restaurants through culture-based methods for detection of Campylobacter motility. Also, molecular tools were used for genetic amplification by PCR using specific primers of hipO gene. Contamination with C. jejuni was recorded in 21.5% in chickens (16.6% in grill tissues and 26.2%...

Prevalence, molecular detection, and virulence gene profiles of Campylobacter species in humans and foods of animal origin

July-2020, 2020

Background and Aim: Campylobacteriosis is one of the most well-characterized bacterial foodborne ... more Background and Aim: Campylobacteriosis is one of the most well-characterized bacterial foodborne infections worldwide that arise chiefly due to the consumption of foods of animal origin such as poultry, milk, and their products. The disease is caused by numerous species within the genus Campylobacter, but Campylobacter jejuni is the most commonly isolated species from established cases of human campylobacteriosis. This study was conducted to determine the prevalence and virulence of Campylobacter isolates from human, chicken, and milk and milk products in Egypt. Materials and Methods: A total of 1299 samples (547 chicken intestine and liver, 647 milk and milk products, and 105 human stool) were collected and microbiologically investigated, confirmed by multiplex polymerase chain reaction (PCR) targeting the 23S rRNA, hipO, and glyA genes specific for Campylobacter spp., C. jejuni, and Campylobacter Coli, respectively, followed by virulence genes (Campylobacter adhesion to fibronecti...

$Research paper thumbnail of {"__content__"=>"Serological and molecular diagnosis of in patients with schizophrenia.", "i"=>{"__content__"=>"Toxoplasma gondii"}}$

{"__content__"=>"Serological and molecular diagnosis of in patients with schizophrenia.", "i"=>{"__content__"=>"Toxoplasma gondii"}}

Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2018

Schizophrenia is a persistent neuropsychiatric syndrome of uncertain source. Toxoplasmosis is the... more Schizophrenia is a persistent neuropsychiatric syndrome of uncertain source. Toxoplasmosis is the most prevalent parasitic protozoan infecting one-third of the worldwide human population. Infectious agents such as toxoplasma are the probable cause of schizophrenia. This study was aimed to evaluate the association between schizophrenia and toxoplasmosis using SAG1 and B1 Target gene. During February to December 2016, 92 patients with schizophrenia are imported in our study. All cases were assessed by serological (IgG and IgM antibodies) and molecular examinations. ELISA was performed by Commercial kits according to manufactures procedure. DNA was extracted and nested PCR was done using two pairs of primers. From 92 patients, 59 (64.13%) cases were positive for toxoplasmosis by serological examinations (14 samples positive for IgM and IgG, 40 samples positive for only IgG and 5 samples Positive for only IgM) and 58 (63.04%) were positive by Nested PCR technique. Based on the nested PC...

Toxoplasma gondii parasite load elevation in diabetic rats as latent opportunistic character

Annals of Tropical Medicine and Public Health, Mar 1, 2014

Introduction: Toxoplasma gondii; is extreme zoonotic intracellular protozoan, exceeding human med... more Introduction: Toxoplasma gondii; is extreme zoonotic intracellular protozoan, exceeding human medical impact due congenital and cerebral toxoplasmosis. Diabetes is characterized by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both, possible through its long depilating term to flare up the intrinsic (latent) stage of T. gondii. Objective: Is focused on studying the latent toxoplasmosis, based on identifying levels of both average brain parasite load and immunoglobulin titers in diabetic induced rat. Materials and Methods: A total number of 60 Wistar male rats (120-180 g) were divided into four groups, 15 each and experimented as follow; un-infected-normoglycaemic, un-infected-diabetic, infected-normoglycaemic and infected-diabetic groups. Blood samples were taken from rats for glucose determination and for serological assay, and at the same time brain tissue samples for parasite load estimation were collected from three sacrificed rats in each group at sequence days; 1, 15, 30, 45 and 60 cycle to days post diabetes, with the same compatible days at 45, 60, 75, 90 and 105 corresponding to days post infection. Results: Showed significance higher in both blood glucose levels and in cysts number in infected diabetic group than the uninfected-diabetic one. The elevated brain parasite load and IgM titer were always exceeding higher progression all over the course and parallel to the elevated glucose levels in infected diabetic group. Conclusion: Hyperglycemic factor was confirmed to opportunist higher T. gondii brain parasite load and elevate immunoglobulin titer in latent infected and diabetes induced rat, possible match with T. gondii sero-positive diabetic patient, and reflects the importance of serological monitoring in particular uncontrolled hyperglycemic ones.

Public Health Hazard of Zoonotic Campylobacter jejuni Reference to Egyptian Regional and Seasonal Variations

Research Journal of Microbiology, 2015

Several Campylobacter species are known to be pathogenic to humans, with Campylobacter jejuni bei... more Several Campylobacter species are known to be pathogenic to humans, with Campylobacter jejuni being the main leading cause of campylobacteriosis worldwide. The present investigation aimed to detect C. jejuni from chicken, water, milk and milk products and humans among 4 Egyptian Governorates (Cairo, Fayoum, Minya and Qalubiya) using conventional method and PCR 146 C. jejuni isolates with an incidence of 6.2% were confirmed to species level by polymerase chain reaction through detection of MapA gene. high incidence of C. jejuni was recorded in chicken intestine (12.8%) followed by Chicken farms water (12%), raw chicken meat (9.6%), occupational human workers stool samples (8.4%) then raw milk (2%), Quraish cheese (1.7%) and finally it was 1.2% in yoghourt. The PCR was definitive, reliable method that facilitated rapid identification of C. jejuni to the species level. It concluded that poor hygiene and sanitation in poultry farms could explain this high level of prevalence of C. jejuni among the examined samples.

Corticosteroids Opportunist Higher Toxoplasma gondii Brain Cysts in Latent Infected Mice

International Journal of Zoological Research, 2015

Toxoplasma gondii is an opportunistic zoonotic protozoan, distinguish superior brain parasite loa... more Toxoplasma gondii is an opportunistic zoonotic protozoan, distinguish superior brain parasite load in immune-suppressed patients. Corticosteroids are popular anti-inflammatory with immune-suppressive long course, it possible opportunist higher T. gondii brain parasite load and reverts encephalitis in latent infected personals. The present study concerns this concept and preferred for recognize different levels of T. gondii brain parasite load and immunoglobulin titers in both corticosteroids treated and untreated latent infected mice groups. A total number of 70 Swiss-Webster mice (12-18 g) were divided into four groups, the first and second ones are 30 each (infected-untreated and infected-treated group), the third and fourth 5 each (uninfected-untreated and uninfected-treated control). Administration of glucocorticoid (hydrocortisone sodium succinate) at a dose of 50 mg kgG 1 (I.M) injection 3 times a week with oral administration of dexamethasone sodium phosphate in dose of 2.5 mg kgG 1 dayG 1 per mouse in drinking water for sequence 2 months. The 10 3 bradyzoites from mice brain of cystogenic ME-49 strain was used for inducing latent infected mice groups at 30 days before corticosteroids therapy. Serum and brain tissue samples were collected for serological assay and parasite load estimation from sacrificed mice. The results showed significance elevation of average percent of brain parasite load and IgM/IgG titers. All values exceeds higher and parallel to the progression of corticosteroids term in infected treated group than the infected-untreated one. In conclusion, long-term corticosteroids therapy possible opportunist higher T. gondii brain parasite load and induce encephalitis in latent infected murine model, imitate this serious condition in T. gondii infected patients who received corticosteroids therapy.

Zoonotic Hazards T. gondii Viable Cysts in Ready to Eat Egyptian Meat-Meals

World Journal of Medical Sciences

Toxoplasma gondii, a meat cyst-forming protozoan, has a potential zoonotic impact sequence to con... more Toxoplasma gondii, a meat cyst-forming protozoan, has a potential zoonotic impact sequence to consumption of undercooked meat or chickens harboring bradyzoites. The present study, explores some common Egyptian meat and chicken meals (413 samples), which are still neglected as a possible source that can maximize the incidence of human toxoplasmosis such as sausages, koffta, kebab, shawerma, basterma and offal, plus to chickens were examined for the persistence of viable T. gondii bradyzoites through direct microscopical examination of digested meat samples followed by mice viability test, plus using PCR for recognition of T. gondii DNA of either viable or unviable bradyzoites. Through the microscopical examination, offals (43.4%), mutton kebabs (42.9%), brain tissue (30.6%) and sausage (26.9%) showed the highest percentage of toxoplasma contamination. While using the mice passage testing, mutton kebabs (29.2%), sausage (21.4%), Core pastrami (18.8%) and brain tissue (18.2%) showed th...

Serological and Hormonal Assays of Murine Materno-Fetal Toxoplasma gondii Infection with Emphasis on Virulent Strains

World Journal of Medical Sciences

The present investigation dealt with studying of T. gondii materno-fetal skill within rat progeni... more The present investigation dealt with studying of T. gondii materno-fetal skill within rat progenies that were borne from acute and chronic experimentally infected pregnant dams. A total number of 108 pathogen and toxoplasma free Sprague-Dawley female rats were used. Ninety dams were used for experimental induced acute and chronic trials. Both acute and chronic infected rats were exposed to the three T. gondii strains; type I, II and III. ELISA IgM / IgG as well as, progesterone / estrogen assays were done parallel to female rats with their survived litters. The results revealed that type I possessed the higher percentage of fetal degeneration, while cystogenic II and III showed lower values. We can conclude that successful recognition of T. gondii materno-fetal transmission resulted in abortion and parasite survival.

Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice

Iranian journal of parasitology, 2013

Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurat... more Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurate. To increase the diagnostic potency of antigens, isolation of their immunogenic fractions could be useful. The current research adopted to obtain an affinity isolated fraction from RH strain using CNBr Sepharose 4B column coupled with infected mice sera helping in detection of IgM and IgG of toxoplasmosis due to RH strain and other strains. The isolated fraction was characterized by SDS-PAGE. Moreover, the diagnostic potency of the fraction was assessed by indirect ELISA in mice experimentally infected with RH strain and two other local strains; one of sheep origin and the other of human origin. The fraction was found to be consisted of a single band of 116 kDa compared with 17 bands ranged from 116 to 16 kDa associated with crude extract. The fraction proved potent diagnostic potentials of acute and chronic mice toxoplasmosis. Where it was detected both IgM and IgG antibodies as early...

Public health importance of foodborne pathogens

Foodborne pathogens cause a considerable public health burden and challenge. They cause illnesses... more Foodborne pathogens cause a considerable public health burden and challenge. They cause illnesses and deaths in all populations, particularly in groups at risk such as infants, children, elderly and immunocompromised persons. Diarrheal diseases, almost all of which are caused by foodborne or waterborne microbial pathogens, are leading causes of illness and death in less developed countries, killing an estimated 1.9 million people annually at the global level. Even in developed countries, it is estimated that up to one third of the population is affected by microbiological foodborne diseases each year. The majority of the pathogens causing this significant disease burden are now considered to be zoonotic. The occurrence of some of these zoonotic pathogens seems to have increased significantly over recent years. The most important source of foodborne disease is raw or improperly cooked food (meat and poultry, raw eggs, unpasteurized milk, shellfish and rice). Food handlers play a major role in ensuring food safety throughout the chain of food production. The most commonly recognized foodborne infections are those caused by bacteria (Campylobacter spp., Salmonella spp., E. coli O157:H7, L. monocytogenes); viruses (Hepatitis A virus, Hepatitis E virus, Rotavirus); mycotoxins; marine bio toxins and parasites (T. solium, T. saginata, Echinococcus spp., Trich. spiralis, Fasciola, Cryptosporidium parvum, Entamoeba histolytica, T gondii). Symptoms of foodborne diseases greatly depend on the type of pathogen. Numerous organisms cause similar symptoms, especially diarrhea, abdominal cramps and nausea. Pathogens that cause foodborne sporadic diseases and outbreaks are usually detected by specific laboratory tests. The primary goal of foodborne outbreak investigation is to control ongoing and future outbreaks. The general strategy of prevention of foodborne diseases require the application of surveillance and information systems for these diseases and Hazard Analysis and Critical Control Points (HACCP) system in the food production chain from farm to consumers and import/export regulations. Reducing microbiological contamination in meat can be achieved through environmental hygiene, best quality of livestock feed, animal vaccination, mandatory inspection of livestock as well as sanitary standards for slaughterhouses and meat processing plant. Food safety can also be achieved by applying personal hygiene, hygienic handling of food and sanitation of the premises and kitchen utensils. Routine medical and laboratory examination of food handlers should be followed. Planning health education programs for food handlers and consumers are important rules to prevent foodborne diseases.

Molecular Detection and Genotyping of Toxoplasma gondii from Clinical Samples

Toxoplasmosis - Recent Advances, 2012

Toxoplasmosis-Recent Advances 104 2. Molecular diagnostics 2.1. Methodology Conventional PCR was,... more Toxoplasmosis-Recent Advances 104 2. Molecular diagnostics 2.1. Methodology Conventional PCR was, in the beginning, the molecular detection method of choice for the majority of laboratories dealing with the diagnosis of toxoplasmosis and it was based on both in-house protocols and commercial kits (Lavrard et al., 1995). To increase the sensitivity of molecular diagnostics of toxoplasmosis nested PCR was introduced, although in recent years real-time PCR has shown a significantly higher sensitivity as well as specificity

Elicitation of Subjective Probability Distributions

To incorporate expert opinion into a Bayesian analysis, it must be quantified as a prior distribu... more To incorporate expert opinion into a Bayesian analysis, it must be quantified as a prior distribution through an elicitation process that asks the expert meaningful questions whose answers determine this distribution. The aim of this thesis is to fill some gaps in the available techniques for eliciting prior distributions for Generalized Linear Models (GLMs) and multinomial models. A general method for quantifying opinion about GLMs was developed in Garthwaite and Al-Awadhi (2006). They model the relationship between each continuous predictor and the dependant variable as a piecewise-linear function with a regression coefficient at each of its dividing points. However, coefficients were assumed a priori independent if associated with different predictors. We relax this simplifying assumption and propose three new methods for eliciting positive-definite variance-covariance matrices of a multivariate normal prior distribution. In addition, we extend the method of Garthwaite and Dickey...

In vitro Effects of Some Bee Products on T. gondii Tachyzoites

Toxoplasma gondii, is ideal opportunistic zoonotic protozoan, exceeds neurological and congenital... more Toxoplasma gondii, is ideal opportunistic zoonotic protozoan, exceeds neurological and congenital impact sequence to flare up of latent toxoplasmosis. It is intracellular pathogen and is so difficult to treat, it requires higher doses of intracellular antimicrobial drugs which reflect repulsive side actions. So, the aim of a current study is focused to evaluate the effect of some bee products (propolis, honey and bee venom) with special emphasis to sulfa-dugs as anti-Toxoplasma activity. The results revealed that all investigated bee products showed destructive on viable tachyzoites which promoted directly with the different dilutions of the bee products, in particular; 100, 50, 25 and 10 respectively, at chilling temperature (4°C) and corresponding to LD & LD. Bee venom proofed the faster and powerful in vitro anti-tachyzoites action with all dilutions, 50 100 exceeding even the control sulfonamides. 5, 6, 7 and 8 respectively while diluted propolis were effective to enhance the persisting viable tachyzoites course. Where 7% dilution of propolis symbolizes the maximum optimal value, successfully increases the average percent of LD &LD viable tachyzoites longer time a live 50 100 up to 172.9 hours post exposure to propolis, exceeding about three days over the normal control saline solution. In conclusion: the results revealed that bee products are promising for development of potent and novel antitoxoplasma drug.

Serological and molecular diagnosis of T . gondii in complicated pregnant Egyptian women

In pregnant women, primary Toxoplasma infection or reactivated chronic infection can lead to tran... more In pregnant women, primary Toxoplasma infection or reactivated chronic infection can lead to transplacental transmission with potential risk of congenital toxoplasmosis depending on the stage of pregnancy. Blood and matched placental and /or fetal tissue samples were collected from one hundred and six Egyptian women from the labor ward of Beni-Suef General Hospital and Kasr El-Eini HospitalEgypt, which are suffering from complicated pregnancy. The study includes G1, G2, G3 and G4 are symbolized the abortion corresponding to the 1st, 2nd and 3nd trimesters with congenital anomalies. All women were assayed serologically by IgM and IgG ELISA and molecular identification by PCR for confirming T.gondii infection. The sero-positive total percentage was 42.4%, 35% and 33% using ELISA, Latex agglutination and PCR respectively.It was concluded that all T.gondii sero-positive women were harboring DNA or compatible placental cysts; denote that serological assay was not accurate alone for confi...

Advanced epidemiological studies on toxoplasmosis in animals and man

Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular To... more Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular Toxoplasma gondii protozoan capable of infecting all types of nucleated mammalian host cells. The disease is of economic veterinary importance and of public health hazard in human side. In the present study, antigens were successfully prepared from RH strain tachyzoites and were used for sero-diagnosis of random samples of both human& buffaloes by ELISA and SFDT. Where, the percent of sero-positive was [56.2&50] and [14.6&10.1] respectively. These results were compared with the commercial LAT that gave uncorrelated results and judged as unsuitable test for diagnostic purposes. New techniques were used for developing the performance of maintenance and preservation of RH strain tachyzoites. A modified method for long term acute stage in mice was used for the first time and succeeds to harboring the T. gondii protozoan up to 12 DPI. Also, it was proven that the liquid nitrogen preservation is...

THERAPEUTIC EFFECT OF NITAZOXANIDE AND ZINGIBER OFFICINALE IN TREATMENT OF ACUTE TOXOPLASMOSIS GONDII USING MURINE EXPERIMENTAL MODEL By

Toxoplasmosis is a cosmopolitan parasitic disease that impacts enormous population sector. In thi... more Toxoplasmosis is a cosmopolitan parasitic disease that impacts enormous population sector. In this work we investigated the antitoxoplamic therapeutic effect of one synthetic drug; nitazoxanide (NTZ) and another agent of plant origin; Zingiber officinale (ginger) as adjuvant to the traditional antotoxoplamic drugs (pyrimethamine & sulphadiazine) on experimental murine toxoplasmosis (RH virulent strain). Animals were classified into 7 groups; GI: Non-infected, nontreated (normal control), GII: Infected non -treated mice (infected control), GIII: Infected & treated by a combination of pyrimethamine & sulphadiazine (drug control), Group IV: Infected & treated by Zingiber officinale GV: Infected & treated by nitazoxanide, GVI: Infected & treated by combination of Zingiber officinale and pyrimethamine & sulphadiazine, Group VII: Infected & treated by combination of Zingiber officinale & nitazoxanide. For assessment of the therapeutic effect of various drug regimens on acute toxoplasmosis...

Zoonotic Hazards of Campylobacteriosis in some areas in Egypt

A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of pat... more A total of 2130 samples collected from diarrhea chicken, raw milk, milk products and stool of patient with diarrhea from Menia, Fayoum, Cairo and Qaluobya in Egypt. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hippuricase gene. The overall prevalence of Campylobacter jejuni in intestine and liver of chicken were 40.4 % and 37.5 % respectively, 30% tape water, 4.44% raw milk, Karish cheese and yoghurt 6.66% and 13.33% respectively and 70 (35%) children stool. The positive results of C.jejuni were detected by IFT expressed by green fluorescence staining. PCR amplification of hipO gene of C. jejuni isolated from the clinically diseased chicken and the environmental samples have shown identical fingerprints with human isolates at 344bp, indicating the zoonotic hazards of Campylobacter jejuni in Egypt. [Barakat A. M. A. Mona M. Sobhy; E...

Zoonotic concern of Campylobacter jejuni in raw and ready-to-eat barbequechickens along with Egyptian handlers and consumers via molecular andimmunofluorescent characterization

Raw and under cooked barbeque chickenswhich harboring pathogens of public health and zoonotic imp... more Raw and under cooked barbeque chickenswhich harboring pathogens of public health and zoonotic impact concerning consumers and handler employees. Campylobacter jejuni considers one of the most prevalent chickenborne gastroenteric bacteria. This study spots light on this concept to indemnify zoonotic hazard of C. jejuni by molecular characterization and indirect fluorescent of Egyptian isolates from both chickens and human in contact. From various Egyptian governorates and clinics a total of 588 chicken visceral contents, eviscerated raw and barbeque chickens were collected from different restaurants. Plus, 96 samples from both symptomatic consumers with history of chickens poisoning and chicken handler. Samples were subjected to standard phenotypic identification of C.jejuni, and subsequently immunofluorescent technique (IFT) identification and genetic amplification by PCR using specific primers of hipO gene. The positive results were detected by IFT expressed by green fluorescence s...

Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinella spiralis

Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from ... more Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from toxoplasmosis or trichinellosis may present with the same clinical signs. A total of 166 serum samples from suspected cases of toxoplasmosis were collected (Egypt). ELISA results showed that 54.21% of serum samples were positive for Toxoplasma and 90% of the positive anti-Toxoplasma patient sera were also positive for T. spiralis larval antigen. SDS profiles of the two parasites antigens displayed common bands at 130.95, 116.6, 66.2 and 55.38 KDa. Also, the immunoblot profiles of T. gondii and T. spiralis antigens blotted with ELISA positive human sera showed common immunoreactive bands at 130.95., 113.60, 55.69 and 28.42 KDa. We can conclude that there is a strong cross reactivity between the two parasites and some of these proteins could be useful in developing vaccines or drugs for controlling the diseases caused by the two parasites. Also, we may suggest that 94.20, 36.34 and 33.05 KDa and 64.33 and 44.57 KDa are specific bands for T. gondii and T. spiralis, respectively.

Ecological and Pathological Study of T. gondii Egyptian Rat Isolates Reference to Biological & Genetic Typescripts

Toxoplasma gondii is zoonotic opportunistic protozoan of public health impact. It has usual clona... more Toxoplasma gondii is zoonotic opportunistic protozoan of public health impact. It has usual clonal population of three virulent human and animal types: I, II, and III. The commercial rats are frequently harboring the dormant T. gondii tissue cysts in their tissues. So, rats consider the major prey for outdoor cats that regularly stimulating it to shed and contaminate the environment with oocysts infective stage which maximizes human and animals' toxoplasmosis. The aim of the present study is to identify the biological and molecular typescripts of naturally infected commercial rats along with serological, mice viability and histo-pathological assays for clarifying to how extent the indirect zoonotic bio-hazards sequence to rats harboring T. gondiivirulent types. A total number of 278 commercial rats were recognized as R. norvegicus (n=74), Rattus rattus rattus(n=108)and Rattus rattus frugivorus (n=96), were collected from different rural and urban sites from Cairo and Giza govern...

Incidence of zoonotic Campylobacter jejuni in fast meal meat, grill chickens and symptomatic Egyptians

African Journal of Microbiology Research, 2016

Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains ... more Campylobacter jejuni is one of the most important foodborne gastroenteric zoonosis. Most strains of C. jejuni produce a toxin (cytolethal distending toxin) that hinders the cells from dividing and produces diffuse bloody edematous exudative enteritis. The common routes of transmission are fecal-oral, person-to-person and the eating of raw or undercooked chickens or meat. This study recognizes the incidence of zoonotic C. jejuni in under cooked chickens and meat meals along with persons in contact. We examined 640 grilled chickens and 733 fast meat meals, plus 93 of symptomatic consumers and handlers were collected from five Egyptian governorates (Fayuom, Cairo, Qaluobia, Bin-suef and Assuit) from different restaurants through culture-based methods for detection of Campylobacter motility. Also, molecular tools were used for genetic amplification by PCR using specific primers of hipO gene. Contamination with C. jejuni was recorded in 21.5% in chickens (16.6% in grill tissues and 26.2%...

Prevalence, molecular detection, and virulence gene profiles of Campylobacter species in humans and foods of animal origin

July-2020, 2020

Background and Aim: Campylobacteriosis is one of the most well-characterized bacterial foodborne ... more Background and Aim: Campylobacteriosis is one of the most well-characterized bacterial foodborne infections worldwide that arise chiefly due to the consumption of foods of animal origin such as poultry, milk, and their products. The disease is caused by numerous species within the genus Campylobacter, but Campylobacter jejuni is the most commonly isolated species from established cases of human campylobacteriosis. This study was conducted to determine the prevalence and virulence of Campylobacter isolates from human, chicken, and milk and milk products in Egypt. Materials and Methods: A total of 1299 samples (547 chicken intestine and liver, 647 milk and milk products, and 105 human stool) were collected and microbiologically investigated, confirmed by multiplex polymerase chain reaction (PCR) targeting the 23S rRNA, hipO, and glyA genes specific for Campylobacter spp., C. jejuni, and Campylobacter Coli, respectively, followed by virulence genes (Campylobacter adhesion to fibronecti...

$Research paper thumbnail of {"__content__"=>"Serological and molecular diagnosis of in patients with schizophrenia.", "i"=>{"__content__"=>"Toxoplasma gondii"}}$

{"__content__"=>"Serological and molecular diagnosis of in patients with schizophrenia.", "i"=>{"__content__"=>"Toxoplasma gondii"}}

Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2018

Schizophrenia is a persistent neuropsychiatric syndrome of uncertain source. Toxoplasmosis is the... more Schizophrenia is a persistent neuropsychiatric syndrome of uncertain source. Toxoplasmosis is the most prevalent parasitic protozoan infecting one-third of the worldwide human population. Infectious agents such as toxoplasma are the probable cause of schizophrenia. This study was aimed to evaluate the association between schizophrenia and toxoplasmosis using SAG1 and B1 Target gene. During February to December 2016, 92 patients with schizophrenia are imported in our study. All cases were assessed by serological (IgG and IgM antibodies) and molecular examinations. ELISA was performed by Commercial kits according to manufactures procedure. DNA was extracted and nested PCR was done using two pairs of primers. From 92 patients, 59 (64.13%) cases were positive for toxoplasmosis by serological examinations (14 samples positive for IgM and IgG, 40 samples positive for only IgG and 5 samples Positive for only IgM) and 58 (63.04%) were positive by Nested PCR technique. Based on the nested PC...

Toxoplasma gondii parasite load elevation in diabetic rats as latent opportunistic character

Annals of Tropical Medicine and Public Health, Mar 1, 2014

Introduction: Toxoplasma gondii; is extreme zoonotic intracellular protozoan, exceeding human med... more Introduction: Toxoplasma gondii; is extreme zoonotic intracellular protozoan, exceeding human medical impact due congenital and cerebral toxoplasmosis. Diabetes is characterized by hyperglycemia resulting from perturbation in insulin secretion, insulin action or both, possible through its long depilating term to flare up the intrinsic (latent) stage of T. gondii. Objective: Is focused on studying the latent toxoplasmosis, based on identifying levels of both average brain parasite load and immunoglobulin titers in diabetic induced rat. Materials and Methods: A total number of 60 Wistar male rats (120-180 g) were divided into four groups, 15 each and experimented as follow; un-infected-normoglycaemic, un-infected-diabetic, infected-normoglycaemic and infected-diabetic groups. Blood samples were taken from rats for glucose determination and for serological assay, and at the same time brain tissue samples for parasite load estimation were collected from three sacrificed rats in each group at sequence days; 1, 15, 30, 45 and 60 cycle to days post diabetes, with the same compatible days at 45, 60, 75, 90 and 105 corresponding to days post infection. Results: Showed significance higher in both blood glucose levels and in cysts number in infected diabetic group than the uninfected-diabetic one. The elevated brain parasite load and IgM titer were always exceeding higher progression all over the course and parallel to the elevated glucose levels in infected diabetic group. Conclusion: Hyperglycemic factor was confirmed to opportunist higher T. gondii brain parasite load and elevate immunoglobulin titer in latent infected and diabetes induced rat, possible match with T. gondii sero-positive diabetic patient, and reflects the importance of serological monitoring in particular uncontrolled hyperglycemic ones.

Public Health Hazard of Zoonotic Campylobacter jejuni Reference to Egyptian Regional and Seasonal Variations

Research Journal of Microbiology, 2015

Several Campylobacter species are known to be pathogenic to humans, with Campylobacter jejuni bei... more Several Campylobacter species are known to be pathogenic to humans, with Campylobacter jejuni being the main leading cause of campylobacteriosis worldwide. The present investigation aimed to detect C. jejuni from chicken, water, milk and milk products and humans among 4 Egyptian Governorates (Cairo, Fayoum, Minya and Qalubiya) using conventional method and PCR 146 C. jejuni isolates with an incidence of 6.2% were confirmed to species level by polymerase chain reaction through detection of MapA gene. high incidence of C. jejuni was recorded in chicken intestine (12.8%) followed by Chicken farms water (12%), raw chicken meat (9.6%), occupational human workers stool samples (8.4%) then raw milk (2%), Quraish cheese (1.7%) and finally it was 1.2% in yoghourt. The PCR was definitive, reliable method that facilitated rapid identification of C. jejuni to the species level. It concluded that poor hygiene and sanitation in poultry farms could explain this high level of prevalence of C. jejuni among the examined samples.

Corticosteroids Opportunist Higher Toxoplasma gondii Brain Cysts in Latent Infected Mice

International Journal of Zoological Research, 2015

Toxoplasma gondii is an opportunistic zoonotic protozoan, distinguish superior brain parasite loa... more Toxoplasma gondii is an opportunistic zoonotic protozoan, distinguish superior brain parasite load in immune-suppressed patients. Corticosteroids are popular anti-inflammatory with immune-suppressive long course, it possible opportunist higher T. gondii brain parasite load and reverts encephalitis in latent infected personals. The present study concerns this concept and preferred for recognize different levels of T. gondii brain parasite load and immunoglobulin titers in both corticosteroids treated and untreated latent infected mice groups. A total number of 70 Swiss-Webster mice (12-18 g) were divided into four groups, the first and second ones are 30 each (infected-untreated and infected-treated group), the third and fourth 5 each (uninfected-untreated and uninfected-treated control). Administration of glucocorticoid (hydrocortisone sodium succinate) at a dose of 50 mg kgG 1 (I.M) injection 3 times a week with oral administration of dexamethasone sodium phosphate in dose of 2.5 mg kgG 1 dayG 1 per mouse in drinking water for sequence 2 months. The 10 3 bradyzoites from mice brain of cystogenic ME-49 strain was used for inducing latent infected mice groups at 30 days before corticosteroids therapy. Serum and brain tissue samples were collected for serological assay and parasite load estimation from sacrificed mice. The results showed significance elevation of average percent of brain parasite load and IgM/IgG titers. All values exceeds higher and parallel to the progression of corticosteroids term in infected treated group than the infected-untreated one. In conclusion, long-term corticosteroids therapy possible opportunist higher T. gondii brain parasite load and induce encephalitis in latent infected murine model, imitate this serious condition in T. gondii infected patients who received corticosteroids therapy.

Zoonotic Hazards T. gondii Viable Cysts in Ready to Eat Egyptian Meat-Meals

World Journal of Medical Sciences

Toxoplasma gondii, a meat cyst-forming protozoan, has a potential zoonotic impact sequence to con... more Toxoplasma gondii, a meat cyst-forming protozoan, has a potential zoonotic impact sequence to consumption of undercooked meat or chickens harboring bradyzoites. The present study, explores some common Egyptian meat and chicken meals (413 samples), which are still neglected as a possible source that can maximize the incidence of human toxoplasmosis such as sausages, koffta, kebab, shawerma, basterma and offal, plus to chickens were examined for the persistence of viable T. gondii bradyzoites through direct microscopical examination of digested meat samples followed by mice viability test, plus using PCR for recognition of T. gondii DNA of either viable or unviable bradyzoites. Through the microscopical examination, offals (43.4%), mutton kebabs (42.9%), brain tissue (30.6%) and sausage (26.9%) showed the highest percentage of toxoplasma contamination. While using the mice passage testing, mutton kebabs (29.2%), sausage (21.4%), Core pastrami (18.8%) and brain tissue (18.2%) showed th...

Serological and Hormonal Assays of Murine Materno-Fetal Toxoplasma gondii Infection with Emphasis on Virulent Strains

World Journal of Medical Sciences

The present investigation dealt with studying of T. gondii materno-fetal skill within rat progeni... more The present investigation dealt with studying of T. gondii materno-fetal skill within rat progenies that were borne from acute and chronic experimentally infected pregnant dams. A total number of 108 pathogen and toxoplasma free Sprague-Dawley female rats were used. Ninety dams were used for experimental induced acute and chronic trials. Both acute and chronic infected rats were exposed to the three T. gondii strains; type I, II and III. ELISA IgM / IgG as well as, progesterone / estrogen assays were done parallel to female rats with their survived litters. The results revealed that type I possessed the higher percentage of fetal degeneration, while cystogenic II and III showed lower values. We can conclude that successful recognition of T. gondii materno-fetal transmission resulted in abortion and parasite survival.

Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice

Iranian journal of parasitology, 2013

Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurat... more Serological diagnosis of Toxoplasma gondii infection using crude antigens may not be more accurate. To increase the diagnostic potency of antigens, isolation of their immunogenic fractions could be useful. The current research adopted to obtain an affinity isolated fraction from RH strain using CNBr Sepharose 4B column coupled with infected mice sera helping in detection of IgM and IgG of toxoplasmosis due to RH strain and other strains. The isolated fraction was characterized by SDS-PAGE. Moreover, the diagnostic potency of the fraction was assessed by indirect ELISA in mice experimentally infected with RH strain and two other local strains; one of sheep origin and the other of human origin. The fraction was found to be consisted of a single band of 116 kDa compared with 17 bands ranged from 116 to 16 kDa associated with crude extract. The fraction proved potent diagnostic potentials of acute and chronic mice toxoplasmosis. Where it was detected both IgM and IgG antibodies as early...

Public health importance of foodborne pathogens

Foodborne pathogens cause a considerable public health burden and challenge. They cause illnesses... more Foodborne pathogens cause a considerable public health burden and challenge. They cause illnesses and deaths in all populations, particularly in groups at risk such as infants, children, elderly and immunocompromised persons. Diarrheal diseases, almost all of which are caused by foodborne or waterborne microbial pathogens, are leading causes of illness and death in less developed countries, killing an estimated 1.9 million people annually at the global level. Even in developed countries, it is estimated that up to one third of the population is affected by microbiological foodborne diseases each year. The majority of the pathogens causing this significant disease burden are now considered to be zoonotic. The occurrence of some of these zoonotic pathogens seems to have increased significantly over recent years. The most important source of foodborne disease is raw or improperly cooked food (meat and poultry, raw eggs, unpasteurized milk, shellfish and rice). Food handlers play a major role in ensuring food safety throughout the chain of food production. The most commonly recognized foodborne infections are those caused by bacteria (Campylobacter spp., Salmonella spp., E. coli O157:H7, L. monocytogenes); viruses (Hepatitis A virus, Hepatitis E virus, Rotavirus); mycotoxins; marine bio toxins and parasites (T. solium, T. saginata, Echinococcus spp., Trich. spiralis, Fasciola, Cryptosporidium parvum, Entamoeba histolytica, T gondii). Symptoms of foodborne diseases greatly depend on the type of pathogen. Numerous organisms cause similar symptoms, especially diarrhea, abdominal cramps and nausea. Pathogens that cause foodborne sporadic diseases and outbreaks are usually detected by specific laboratory tests. The primary goal of foodborne outbreak investigation is to control ongoing and future outbreaks. The general strategy of prevention of foodborne diseases require the application of surveillance and information systems for these diseases and Hazard Analysis and Critical Control Points (HACCP) system in the food production chain from farm to consumers and import/export regulations. Reducing microbiological contamination in meat can be achieved through environmental hygiene, best quality of livestock feed, animal vaccination, mandatory inspection of livestock as well as sanitary standards for slaughterhouses and meat processing plant. Food safety can also be achieved by applying personal hygiene, hygienic handling of food and sanitation of the premises and kitchen utensils. Routine medical and laboratory examination of food handlers should be followed. Planning health education programs for food handlers and consumers are important rules to prevent foodborne diseases.

Molecular Detection and Genotyping of Toxoplasma gondii from Clinical Samples

Toxoplasmosis - Recent Advances, 2012

Toxoplasmosis-Recent Advances 104 2. Molecular diagnostics 2.1. Methodology Conventional PCR was,... more Toxoplasmosis-Recent Advances 104 2. Molecular diagnostics 2.1. Methodology Conventional PCR was, in the beginning, the molecular detection method of choice for the majority of laboratories dealing with the diagnosis of toxoplasmosis and it was based on both in-house protocols and commercial kits (Lavrard et al., 1995). To increase the sensitivity of molecular diagnostics of toxoplasmosis nested PCR was introduced, although in recent years real-time PCR has shown a significantly higher sensitivity as well as specificity

صور دكتور حسن الفضالى

Hassan Elfadaly صورة الاستاذ الدكتور حسن على الفضالى

Dr. Hassan Elfadaly Curriculum vitae

Hassan Ali Elfadaly Curriculum vitae Born on 22 /5/ 1967 in Cairo, Egypt. Career Assistant Res... more Hassan Ali Elfadaly Curriculum vitae
Born on 22 /5/ 1967 in Cairo, Egypt.

Career
Assistant Research Professor, medical educator (veterinary division). National Research Center, Giza.
Education
Master Degree of Vet. Sci. (zoonosis), Faculty of Vet. Med., Cairo Univ.,, 2002, Cairo University. Epidemiological studies on toxoplasmosis in at risk sheep, goats and women.

Doctor of Philosophy in Veterinary Medicine Ph. D. Degree of Vet. Sci., (zoonosis), Faculty of Vet. Med. Cairo Univ., 2007,.Advanced epidemiological studies on toxoplasmosis in animals and man.

Major achievements
Achievements include researches in zoonotic diseases, public health and food inspection aspects.

Connections
Married Rasheda ibrahem. Children: Ragaa, Esraa, Ali and Yousef.

Favorites
Decoration and painting
Publication

2008

1. Hassanain, M.A.; Ashraf M. Barakat; Hassan A.Elfadaly; Nawal A.Hassanain and Raafat M. Shaapan . (2008): Zoonotic impact of Toxoplasma gondii Sero-prevalance in naturally infected Egyptian kittens. Journal of the Arab Society for Medical Research (JASMR), 3(2): 243-248.

2. M. Shaapan; M.A.Hassanain; Nawal A.Hassanain and H.A.EL-Fadaly.(2008):Induction of Toxoplasma gondii in goats. Egyptian Journal of Veterinary Science (NRC), 42, 1-10.

2009

3. Hassanain, M.A.; Nawal AHassanain, Raafat M Shaapan; Hassan A El Fadalyand Faragalla M El Mogazy(2009): Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinellaspiralis.XIV ISAH (International Society for Animal Hygiene) Congress in Vechta, Germany, 19th – 23rd July 2009.

4. Barakat A.M.A., M.M. Abd-Elaziz and H.A.El-Fadaly (2009) : Comparative diagnosis of Toxoplasmosis in Egytptian small ruminants by Indirect Haemagglutination Assay and ELISA . Global Veterinaria 3(1):09-14.

2010

5. Sobhy M.M., Barakat.A.M.A..,and El-Fadaly H.A. (2010) : Bio-assay on zoonotic Campylobacter jejuni in Egyptian quick meat snacks concerning low and high standard areas. Journal of the Egyptian Veterinary Medical Association, 70(4):645-653.

6. Hassanain N.A.; Mohey, A. Hassanain; Raafat, M. Shaapan; Hassan, A. Fadalyand Ashraf, M. Barakat (2010): Diagnosis of Egyptian Bovine Meat Borne Zoonosis. Journal of American Science 6(12): 1526-1533.

7. Hassanain Mohey, Hassan A . El Fadaly, Ashraf Barakat, Rafaat Shapaan, Nawal Hassanain . (2010): BIOLOGICAL TYPESCRIPT OF T.GONDII EGYPTIAN SHEEP ISOLATES A BIO-INDICATOR FOR MUTTON-BORNE TOXOPLASMOSIS; Proceedings of the XXVI,World Buiatrics Congress,Nov. 14-18, 2010,Santiago de Chile, Chile http://www.ivis.org.
2011

8. Kotb M.H.R; Barakat A.M.A.; Sobhy M.M. and El-Fadaly H.A. (2011) Detection of zoonotic pathogens in untreated ground water in rural Egyptian communities. Journal of the Egyptian Veterinary Medical Association, 71: 15-21.
9. Abd El-Razik. K.A.; A.A. AL-Humiany; W.M. Ahmed; A.M.A. Barakat and H.A. ELfadaly (2011): Investigations on non- Brucella abortifacients insmall ruminants in Saudi Arabia with emphasis on zoonotic causes. Global Veterinaria .6(1):25-32.
10. Ashraf, M. Barakat; Hassan, A. El Fadaly; Raafat, M. Shaapan and Fathia, A.M. Khalil (2011): Occupational Health Hazard of Egyptian Employees in Contact with Wastage Nourished Swine. Journal of American Science 7(1): 808-813.
11. Hassanain, M.A.,;H.A. Elfadaly; R. M. Shaaban ; N.A. Hassanain and A.M. Barakat(2011). Biological Assay of Toxoplasma gondii Egyptian Mutton Isolates.International Journal of Zoological Research 7 (4): 330-337.
2012

12. El Fadaly H. A, Mohey A. Hassanain; Raafat M. Shaapan; Ashraf, M.Barakat and Nagwa I. Toaleb, (2012): Serological and Hormonal Assays of Murine Materno-Fetal Toxoplasma gondii Infection with Emphasis on Virulent Strains. World Journal of Medical Sciences 7 (4): 248-254.

2013

13. Hassanain, M.A.,, Hassan A. El-Fadaly, 1Nawal A. Hassanain,Raafat M. Shaapan, 1Ashraf M. Barakat and 2Khaled A. Abd El-Razik. (2013);Serological and Molecular Diagnosis of Toxoplasmosis in Human and Animals.World Journal of Medical Sciences 9 (4): 243-247.

14. Hassanain N.A., Mohey A. Hassanain, 2Wahid M. Ahmed,Raafat M. Shaapan, 1Ashraf M. Barakat and Hassan A.El-Fadaly. (2013):Public Health Importance of Foodborne Pathogens.World Journal of Medical Sciences 9 (4): 208-222.

15. Hassanain, M.A., ,Eman Hussien ABDEL-RAHMAN , Nagwa Ibrahim TOALEB , *Raafat Mohamed SHAAPAN , Hasan Ali ELFADALY, Nawal Abdel-Hafez HASSANAIN..Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice.. Iranian J Parasitol: Vol. 8, No.3, July -Sep 2013, pp.441-448.

2014

16. Hassanain, M.A.,, Hassan A. El-Fadaly, Nawal A. Hassanain..Toxoplasma gondii parasite load elevation in diabetic rats as latent opportunistic character.Annals of Tropical Medicine and Public Health | Mar-Apr 2014 | Vol 7 | Issue 2

17. Hegazi A. G., Hassan A. El-Fadaly, Ashraf M. Barakat and Sanaa K.A. Abou-El-Doubal.In vitro Effects of Some Bee Products on T. gondiiTachyzoites.Global Veterinaria 13 (6): 1043-1050, 2014.

18. Abd El-Razik. K.A., H.A. El Fadaly, 2A.M.A. Barakat and Azza 3S.M. Abu Elnaga.Zoonotic Hazards T. gondii Viable Cysts in Ready to Eat Egyptian Meat-Meals.World Journal of Medical Sciences 11 (4): 510-517, 2014
2015

19. El Fadaly H. A.Soror A. H., Barakat A. M. A., Abd El-Razik K. A. (2015) ZOONOTIC AND HISTO-PATHOLOGICAL ASPECTS OF VARIOUS TOXOPLASMA GONDII STRAINS IN FEMALE RATS . Afr. J. Infect. Dis. 9(2): 32 – 38 .http://dx.doi.org/10.4314/ajid.v9i2.3.

20. Barakat A. M. A. 1; Mona, M. Sobhy2; El Fadaly H. A.; Nagwa, S. Rabaei3;, Nashwa, M. Othman4; Eman, S.Ramadan2, Kotb, M. H. R.2 and Mona S. Zaki5. (2015) Zoonotic Hazards of Campylobacteriosis in some areas in Egypt. Life Science Journal;12(7).http://www.lifesciencesite.com.

21. Elfadaly H.A., Mohey A. Hassanain, Raafat M. Shaapan, Nawal A. Hassanain and Ashraaf M. Barakat.( 2015) Corticosteroids Opportunist Higher Toxoplasma gondii Brain Cystsin Latent Infected Mice .International Journal of Zoological Research 11 (4): 169-176.

22. HodaAbd El Megid El Bolaky1, Mohey El Din Abd El Hafiz Hassanain,Azza Ibrahim HassanYounis1, EnasYahia Abu- Sarea ,Hassan Ali El Fadaly and Wegdan Mohamed Abd El Wahab. (2015).Bio-typing of Toxoplasma gondii isolate from complicated pregnant Egyptian women. .Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 3(9) pp. 453-458.

23. Hassanain, M.A.,, HodaAbd El Megid El Bolaky2, Azza Ibrahim HassanYounis2, EnasYahia Abu- Sarea3, Khaled A. Abd El-Razik4 , Hassan Ali El Fadaly ,WegdanMohamed Abd El Wahab.(2015) ,Serological and molecular diagnosis of T. gondii in complicated pregnant Egyptian women. Basic Research Journal of Medicine and Clinical Sciences ISSN 2315-6864 Vol. 4(9) pp. 231-236 September 2015.

24. Shimaa T. Omara, H.A. El Fadaly and A.M.A. Barakat (2015).Public Health Hazard of Zoonotic Campylobacter jejuni Reference to Egyptian Regional and Seasonal Variations. Research Journal of Microbiology 10 (8): 343-354, 2015.

25. Raafat Shaapan and Hassan Elfadaly, (2015): Latency in Toxoplasmosis:Amazon.co.uk: 9783659761348: Books Paperback.: LAP Lambert Academic Publishing (5 Aug. 2015).

2016

Hassan Ali Elfadaly Curriculum vitae

Hassan Ali Elfadaly Curriculum vitae Born on 22 /5/ 1967 in Cairo, Egypt. Career Assistant Res... more Hassan Ali Elfadaly Curriculum vitae
Born on 22 /5/ 1967 in Cairo, Egypt.

Career
Assistant Research Professor, medical educator (veterinary division). National Research Center, Giza.
Education
Master Degree of Vet. Sci. (zoonosis), Faculty of Vet. Med., Cairo Univ.,, 2002, Cairo University. Epidemiological studies on toxoplasmosis in at risk sheep, goats and women.

Doctor of Philosophy in Veterinary Medicine Ph. D. Degree of Vet. Sci., (zoonosis), Faculty of Vet. Med. Cairo Univ., 2007,.Advanced epidemiological studies on toxoplasmosis in animals and man.

Major achievements
Achievements include researches in zoonotic diseases, public health and food inspection aspects.

Connections
Married Rasheda ibrahem. Children: Ragaa, Esraa, Ali and Yousef.

Favorites
Decoration and painting
Publication

2008

1. Hassanain, M.A.; Ashraf M. Barakat; Hassan A.Elfadaly; Nawal A.Hassanain and Raafat M. Shaapan . (2008): Zoonotic impact of Toxoplasma gondii Sero-prevalance in naturally infected Egyptian kittens. Journal of the Arab Society for Medical Research (JASMR), 3(2): 243-248.

2. M. Shaapan; M.A.Hassanain; Nawal A.Hassanain and H.A.EL-Fadaly.(2008):Induction of Toxoplasma gondii in goats. Egyptian Journal of Veterinary Science (NRC), 42, 1-10.

2009

3. Hassanain, M.A.; Nawal AHassanain, Raafat M Shaapan; Hassan A El Fadalyand Faragalla M El Mogazy(2009): Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinellaspiralis.XIV ISAH (International Society for Animal Hygiene) Congress in Vechta, Germany, 19th – 23rd July 2009.

4. Barakat A.M.A., M.M. Abd-Elaziz and H.A.El-Fadaly (2009) : Comparative diagnosis of Toxoplasmosis in Egytptian small ruminants by Indirect Haemagglutination Assay and ELISA . Global Veterinaria 3(1):09-14.

2010

5. Sobhy M.M., Barakat.A.M.A..,and El-Fadaly H.A. (2010) : Bio-assay on zoonotic Campylobacter jejuni in Egyptian quick meat snacks concerning low and high standard areas. Journal of the Egyptian Veterinary Medical Association, 70(4):645-653.

6. Hassanain N.A.; Mohey, A. Hassanain; Raafat, M. Shaapan; Hassan, A. Fadalyand Ashraf, M. Barakat (2010): Diagnosis of Egyptian Bovine Meat Borne Zoonosis. Journal of American Science 6(12): 1526-1533.

7. Hassanain Mohey, Hassan A . El Fadaly, Ashraf Barakat, Rafaat Shapaan, Nawal Hassanain . (2010): BIOLOGICAL TYPESCRIPT OF T.GONDII EGYPTIAN SHEEP ISOLATES A BIO-INDICATOR FOR MUTTON-BORNE TOXOPLASMOSIS; Proceedings of the XXVI,World Buiatrics Congress,Nov. 14-18, 2010,Santiago de Chile, Chile http://www.ivis.org.
2011

8. Kotb M.H.R; Barakat A.M.A.; Sobhy M.M. and El-Fadaly H.A. (2011) Detection of zoonotic pathogens in untreated ground water in rural Egyptian communities. Journal of the Egyptian Veterinary Medical Association, 71: 15-21.
9. Abd El-Razik. K.A.; A.A. AL-Humiany; W.M. Ahmed; A.M.A. Barakat and H.A. ELfadaly (2011): Investigations on non- Brucella abortifacients insmall ruminants in Saudi Arabia with emphasis on zoonotic causes. Global Veterinaria .6(1):25-32.
10. Ashraf, M. Barakat; Hassan, A. El Fadaly; Raafat, M. Shaapan and Fathia, A.M. Khalil (2011): Occupational Health Hazard of Egyptian Employees in Contact with Wastage Nourished Swine. Journal of American Science 7(1): 808-813.
11. Hassanain, M.A.,;H.A. Elfadaly; R. M. Shaaban ; N.A. Hassanain and A.M. Barakat(2011). Biological Assay of Toxoplasma gondii Egyptian Mutton Isolates.International Journal of Zoological Research 7 (4): 330-337.
2012

12. El Fadaly H. A, Mohey A. Hassanain; Raafat M. Shaapan; Ashraf, M.Barakat and Nagwa I. Toaleb, (2012): Serological and Hormonal Assays of Murine Materno-Fetal Toxoplasma gondii Infection with Emphasis on Virulent Strains. World Journal of Medical Sciences 7 (4): 248-254.

2013

13. Hassanain, M.A.,, Hassan A. El-Fadaly, 1Nawal A. Hassanain,Raafat M. Shaapan, 1Ashraf M. Barakat and 2Khaled A. Abd El-Razik. (2013);Serological and Molecular Diagnosis of Toxoplasmosis in Human and Animals.World Journal of Medical Sciences 9 (4): 243-247.

14. Hassanain N.A., Mohey A. Hassanain, 2Wahid M. Ahmed,Raafat M. Shaapan, 1Ashraf M. Barakat and Hassan A.El-Fadaly. (2013):Public Health Importance of Foodborne Pathogens.World Journal of Medical Sciences 9 (4): 208-222.

15. Hassanain, M.A., ,Eman Hussien ABDEL-RAHMAN , Nagwa Ibrahim TOALEB , *Raafat Mohamed SHAAPAN , Hasan Ali ELFADALY, Nawal Abdel-Hafez HASSANAIN..Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice.. Iranian J Parasitol: Vol. 8, No.3, July -Sep 2013, pp.441-448.

2014

16. Hassanain, M.A.,, Hassan A. El-Fadaly, Nawal A. Hassanain..Toxoplasma gondii parasite load elevation in diabetic rats as latent opportunistic character.Annals of Tropical Medicine and Public Health | Mar-Apr 2014 | Vol 7 | Issue 2

17. Hegazi A. G., Hassan A. El-Fadaly, Ashraf M. Barakat and Sanaa K.A. Abou-El-Doubal.In vitro Effects of Some Bee Products on T. gondiiTachyzoites.Global Veterinaria 13 (6): 1043-1050, 2014.

18. Abd El-Razik. K.A., H.A. El Fadaly, 2A.M.A. Barakat and Azza 3S.M. Abu Elnaga.Zoonotic Hazards T. gondii Viable Cysts in Ready to Eat Egyptian Meat-Meals.World Journal of Medical Sciences 11 (4): 510-517, 2014
2015

19. El Fadaly H. A.Soror A. H., Barakat A. M. A., Abd El-Razik K. A. (2015) ZOONOTIC AND HISTO-PATHOLOGICAL ASPECTS OF VARIOUS TOXOPLASMA GONDII STRAINS IN FEMALE RATS . Afr. J. Infect. Dis. 9(2): 32 – 38 .http://dx.doi.org/10.4314/ajid.v9i2.3.

20. Barakat A. M. A. 1; Mona, M. Sobhy2; El Fadaly H. A.; Nagwa, S. Rabaei3;, Nashwa, M. Othman4; Eman, S.Ramadan2, Kotb, M. H. R.2 and Mona S. Zaki5. (2015) Zoonotic Hazards of Campylobacteriosis in some areas in Egypt. Life Science Journal;12(7).http://www.lifesciencesite.com.

21. Elfadaly H.A., Mohey A. Hassanain, Raafat M. Shaapan, Nawal A. Hassanain and Ashraaf M. Barakat.( 2015) Corticosteroids Opportunist Higher Toxoplasma gondii Brain Cystsin Latent Infected Mice .International Journal of Zoological Research 11 (4): 169-176.

22. HodaAbd El Megid El Bolaky1, Mohey El Din Abd El Hafiz Hassanain,Azza Ibrahim HassanYounis1, EnasYahia Abu- Sarea ,Hassan Ali El Fadaly and Wegdan Mohamed Abd El Wahab. (2015).Bio-typing of Toxoplasma gondii isolate from complicated pregnant Egyptian women. .Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 3(9) pp. 453-458.

23. Hassanain, M.A.,, HodaAbd El Megid El Bolaky2, Azza Ibrahim HassanYounis2, EnasYahia Abu- Sarea3, Khaled A. Abd El-Razik4 , Hassan Ali El Fadaly ,WegdanMohamed Abd El Wahab.(2015) ,Serological and molecular diagnosis of T. gondii in complicated pregnant Egyptian women. Basic Research Journal of Medicine and Clinical Sciences ISSN 2315-6864 Vol. 4(9) pp. 231-236 September 2015.

24. Shimaa T. Omara, H.A. El Fadaly and A.M.A. Barakat (2015).Public Health Hazard of Zoonotic Campylobacter jejuni Reference to Egyptian Regional and Seasonal Variations. Research Journal of Microbiology 10 (8): 343-354, 2015.

25. Raafat Shaapan and Hassan Elfadaly, (2015): Latency in Toxoplasmosis:Amazon.co.uk: 9783659761348: Books Paperback.: LAP Lambert Academic Publishing (5 Aug. 2015).

2016

Ass. Prof.Hassan A. Elfadaly Curriculum vitae

Hassan Ali Elfadaly Curriculum vitae Born on 22 /5/ 1967 in Cairo, Egypt. Career Assistant Res... more Hassan Ali Elfadaly Curriculum vitae
Born on 22 /5/ 1967 in Cairo, Egypt.

Career
Assistant Research Professor, medical educator (veterinary division). National Research Center, Giza.
Education
Master Degree of Vet. Sci. (zoonosis), Faculty of Vet. Med., Cairo Univ.,, 2002, Cairo University. Epidemiological studies on toxoplasmosis in at risk sheep, goats and women.

Doctor of Philosophy in Veterinary Medicine Ph. D. Degree of Vet. Sci., (zoonosis), Faculty of Vet. Med. Cairo Univ., 2007,.Advanced epidemiological studies on toxoplasmosis in animals and man.

Major achievements
Achievements include researches in zoonotic diseases, public health and food inspection aspects.

Connections
Married Rasheda ibrahem. Children: Ragaa, Esraa, Ali and Yousef.

Favorites
Decoration and painting
Publication

2008

1. Hassanain, M.A.; Ashraf M. Barakat; Hassan A.Elfadaly; Nawal A.Hassanain and Raafat M. Shaapan . (2008): Zoonotic impact of Toxoplasma gondii Sero-prevalance in naturally infected Egyptian kittens. Journal of the Arab Society for Medical Research (JASMR), 3(2): 243-248.

2. M. Shaapan; M.A.Hassanain; Nawal A.Hassanain and H.A.EL-Fadaly.(2008):Induction of Toxoplasma gondii in goats. Egyptian Journal of Veterinary Science (NRC), 42, 1-10.

2009

3. Hassanain, M.A.; Nawal AHassanain, Raafat M Shaapan; Hassan A El Fadalyand Faragalla M El Mogazy(2009): Problem of toxoplasmosis and detection of possible cross-reactivity with Trichinellaspiralis.XIV ISAH (International Society for Animal Hygiene) Congress in Vechta, Germany, 19th – 23rd July 2009.

4. Barakat A.M.A., M.M. Abd-Elaziz and H.A.El-Fadaly (2009) : Comparative diagnosis of Toxoplasmosis in Egytptian small ruminants by Indirect Haemagglutination Assay and ELISA . Global Veterinaria 3(1):09-14.

2010

5. Sobhy M.M., Barakat.A.M.A..,and El-Fadaly H.A. (2010) : Bio-assay on zoonotic Campylobacter jejuni in Egyptian quick meat snacks concerning low and high standard areas. Journal of the Egyptian Veterinary Medical Association, 70(4):645-653.

6. Hassanain N.A.; Mohey, A. Hassanain; Raafat, M. Shaapan; Hassan, A. Fadalyand Ashraf, M. Barakat (2010): Diagnosis of Egyptian Bovine Meat Borne Zoonosis. Journal of American Science 6(12): 1526-1533.

7. Hassanain Mohey, Hassan A . El Fadaly, Ashraf Barakat, Rafaat Shapaan, Nawal Hassanain . (2010): BIOLOGICAL TYPESCRIPT OF T.GONDII EGYPTIAN SHEEP ISOLATES A BIO-INDICATOR FOR MUTTON-BORNE TOXOPLASMOSIS; Proceedings of the XXVI,World Buiatrics Congress,Nov. 14-18, 2010,Santiago de Chile, Chile http://www.ivis.org.
2011

8. Kotb M.H.R; Barakat A.M.A.; Sobhy M.M. and El-Fadaly H.A. (2011) Detection of zoonotic pathogens in untreated ground water in rural Egyptian communities. Journal of the Egyptian Veterinary Medical Association, 71: 15-21.
9. Abd El-Razik. K.A.; A.A. AL-Humiany; W.M. Ahmed; A.M.A. Barakat and H.A. ELfadaly (2011): Investigations on non- Brucella abortifacients insmall ruminants in Saudi Arabia with emphasis on zoonotic causes. Global Veterinaria .6(1):25-32.
10. Ashraf, M. Barakat; Hassan, A. El Fadaly; Raafat, M. Shaapan and Fathia, A.M. Khalil (2011): Occupational Health Hazard of Egyptian Employees in Contact with Wastage Nourished Swine. Journal of American Science 7(1): 808-813.
11. Hassanain, M.A.,;H.A. Elfadaly; R. M. Shaaban ; N.A. Hassanain and A.M. Barakat(2011). Biological Assay of Toxoplasma gondii Egyptian Mutton Isolates.International Journal of Zoological Research 7 (4): 330-337.
2012

12. El Fadaly H. A, Mohey A. Hassanain; Raafat M. Shaapan; Ashraf, M.Barakat and Nagwa I. Toaleb, (2012): Serological and Hormonal Assays of Murine Materno-Fetal Toxoplasma gondii Infection with Emphasis on Virulent Strains. World Journal of Medical Sciences 7 (4): 248-254.

2013

13. Hassanain, M.A.,, Hassan A. El-Fadaly, 1Nawal A. Hassanain,Raafat M. Shaapan, 1Ashraf M. Barakat and 2Khaled A. Abd El-Razik. (2013);Serological and Molecular Diagnosis of Toxoplasmosis in Human and Animals.World Journal of Medical Sciences 9 (4): 243-247.

14. Hassanain N.A., Mohey A. Hassanain, 2Wahid M. Ahmed,Raafat M. Shaapan, 1Ashraf M. Barakat and Hassan A.El-Fadaly. (2013):Public Health Importance of Foodborne Pathogens.World Journal of Medical Sciences 9 (4): 208-222.

15. Hassanain, M.A., ,Eman Hussien ABDEL-RAHMAN , Nagwa Ibrahim TOALEB , *Raafat Mohamed SHAAPAN , Hasan Ali ELFADALY, Nawal Abdel-Hafez HASSANAIN..Development of 116 kDa Fraction for Detecting Experimental Toxoplasma gondii Infections in Mice.. Iranian J Parasitol: Vol. 8, No.3, July -Sep 2013, pp.441-448.

2014

16. Hassanain, M.A.,, Hassan A. El-Fadaly, Nawal A. Hassanain..Toxoplasma gondii parasite load elevation in diabetic rats as latent opportunistic character.Annals of Tropical Medicine and Public Health | Mar-Apr 2014 | Vol 7 | Issue 2

17. Hegazi A. G., Hassan A. El-Fadaly, Ashraf M. Barakat and Sanaa K.A. Abou-El-Doubal.In vitro Effects of Some Bee Products on T. gondiiTachyzoites.Global Veterinaria 13 (6): 1043-1050, 2014.

18. Abd El-Razik. K.A., H.A. El Fadaly, 2A.M.A. Barakat and Azza 3S.M. Abu Elnaga.Zoonotic Hazards T. gondii Viable Cysts in Ready to Eat Egyptian Meat-Meals.World Journal of Medical Sciences 11 (4): 510-517, 2014
2015

19. El Fadaly H. A.Soror A. H., Barakat A. M. A., Abd El-Razik K. A. (2015) ZOONOTIC AND HISTO-PATHOLOGICAL ASPECTS OF VARIOUS TOXOPLASMA GONDII STRAINS IN FEMALE RATS . Afr. J. Infect. Dis. 9(2): 32 – 38 .http://dx.doi.org/10.4314/ajid.v9i2.3.

20. Barakat A. M. A. 1; Mona, M. Sobhy2; El Fadaly H. A.; Nagwa, S. Rabaei3;, Nashwa, M. Othman4; Eman, S.Ramadan2, Kotb, M. H. R.2 and Mona S. Zaki5. (2015) Zoonotic Hazards of Campylobacteriosis in some areas in Egypt. Life Science Journal;12(7).http://www.lifesciencesite.com.

21. Elfadaly H.A., Mohey A. Hassanain, Raafat M. Shaapan, Nawal A. Hassanain and Ashraaf M. Barakat.( 2015) Corticosteroids Opportunist Higher Toxoplasma gondii Brain Cystsin Latent Infected Mice .International Journal of Zoological Research 11 (4): 169-176.

22. HodaAbd El Megid El Bolaky1, Mohey El Din Abd El Hafiz Hassanain,Azza Ibrahim HassanYounis1, EnasYahia Abu- Sarea ,Hassan Ali El Fadaly and Wegdan Mohamed Abd El Wahab. (2015).Bio-typing of Toxoplasma gondii isolate from complicated pregnant Egyptian women. .Merit Research Journal of Medicine and Medical Sciences (ISSN: 2354-323X) Vol. 3(9) pp. 453-458.

23. Hassanain, M.A.,, HodaAbd El Megid El Bolaky2, Azza Ibrahim HassanYounis2, EnasYahia Abu- Sarea3, Khaled A. Abd El-Razik4 , Hassan Ali El Fadaly ,WegdanMohamed Abd El Wahab.(2015) ,Serological and molecular diagnosis of T. gondii in complicated pregnant Egyptian women. Basic Research Journal of Medicine and Clinical Sciences ISSN 2315-6864 Vol. 4(9) pp. 231-236 September 2015.

24. Shimaa T. Omara, H.A. El Fadaly and A.M.A. Barakat (2015).Public Health Hazard of Zoonotic Campylobacter jejuni Reference to Egyptian Regional and Seasonal Variations. Research Journal of Microbiology 10 (8): 343-354, 2015.

25. Raafat Shaapan and Hassan Elfadaly, (2015): Latency in Toxoplasmosis:Amazon.co.uk: 9783659761348: Books Paperback.: LAP Lambert Academic Publishing (5 Aug. 2015).

2016

All cases have been linked through travel to or residence in Saudi Arabia, Qatar, Jordan, and Uni... more

Comparative diagnosis of Toxoplasme gondii in Egytptian small ruminants by Indirect Haemagglutination Assay abd ELISA

Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from ... more Both Toxoplasma gondii and Trichinella spiralis are tissue parasites and patients suffering from toxoplasmosis or trichinellosis may present with the same clinical signs. A total of 166 serum samples from suspected cases of toxoplasmosis were collected (Egypt). ELISA results showed that 54.21% of serum samples were positive for Toxoplasma and 90% of the positive anti-Toxoplasma patient sera were also positive for T. spiralis larval antigen. SDS profiles of the two parasites antigens displayed common bands at 130.95, 116.6, 66.2 and 55.38 KDa. Also, the immunoblot profiles of T. gondii and T. spiralis antigens blotted with ELISA positive human sera showed common immunoreactive bands at 130.95., 113.60, 55.69 and 28.42 KDa. We can conclude that there is a strong cross reactivity between the two parasites and some of these proteins could be useful in developing vaccines or drugs for controlling the diseases caused by the two parasites. Also, we may suggest that 94.20, 36.34 and 33.05 KDa and 64.33 and 44.57 KDa are specific bands for T. gondii and T. spiralis, respectively.

􀀐􀀂􀀄􀀑􀀉􀀒􀀏􀀅􀀓􀀉􀀅􀀔􀀕􀀖􀀕􀀈􀀍􀀂􀀋􀀗􀀕􀀋􀀓􀀋􀀐􀀂􀀄􀀑􀀉􀀒􀀏􀀅􀀓􀀉􀀅􀀔􀀕􀀖􀀕􀀈􀀍􀀂􀀋􀀗􀀕􀀋􀀓􀀋Latency in Toxoplasmosis

􀀁􀀂􀀃􀀄􀀅 􀀆􀀇􀀇􀀈􀀅 􀀉􀀃􀀊􀀊􀀅 􀀋􀀌􀀅 􀀍􀀇􀀎􀀍􀀌􀀏􀀎􀀅 􀀐􀀂􀀌􀀅 􀀌􀀑􀀃􀀒􀀌􀀓􀀃􀀇􀀊􀀇􀀔􀀃􀀍􀀕􀀊􀀅 􀀕􀀎􀀒􀀅 􀀖􀀇􀀇􀀎􀀇􀀐􀀃􀀍􀀅 􀀕􀀄􀀑􀀌􀀍􀀐􀀄􀀅 􀀇􀀗 􀀊􀀕􀀐􀀌􀀎􀀐􀀅 􀀂􀀘􀀓􀀕􀀎􀀅 􀀕􀀎... more 􀀁􀀂􀀃􀀄􀀅 􀀆􀀇􀀇􀀈􀀅 􀀉􀀃􀀊􀀊􀀅 􀀋􀀌􀀅 􀀍􀀇􀀎􀀍􀀌􀀏􀀎􀀅 􀀐􀀂􀀌􀀅 􀀌􀀑􀀃􀀒􀀌􀀓􀀃􀀇􀀊􀀇􀀔􀀃􀀍􀀕􀀊􀀅 􀀕􀀎􀀒􀀅 􀀖􀀇􀀇􀀎􀀇􀀐􀀃􀀍􀀅 􀀕􀀄􀀑􀀌􀀍􀀐􀀄􀀅 􀀇􀀗
􀀊􀀕􀀐􀀌􀀎􀀐􀀅 􀀂􀀘􀀓􀀕􀀎􀀅 􀀕􀀎􀀒􀀅 􀀕􀀎􀀃􀀓􀀕􀀊􀀅 􀀐􀀇􀀙􀀇􀀑􀀊􀀕􀀄􀀓􀀇􀀄􀀃􀀄􀀚􀀅 􀀓􀀌􀀍􀀂􀀕􀀎􀀃􀀄􀀓􀀅 􀀕􀀎􀀒􀀅 􀀗􀀕􀀍􀀐􀀇􀀏􀀄􀀅 􀀃􀀎􀀒􀀘􀀍􀀃􀀎􀀔Toxoplasmosis is ideal latent zoonoses; signify public health impact among humans, all
species of warm-blooded animals and birds (Lovett and Sibley, 2003). Toxoplasma gondii is
obligate latent intracellular tissue cyst-forming protozoan, and is one of the most successful
coccidian on earth known to date. Latency is a pathogen defiance mechanism against the
competent host immunity, the acute stage tachyzoites capable to metamorphosis intracellular
to active bradyzites latent phase (tissue cyst), are slowly replicated, and stay permanent alive
for the host survives (Hill and Dubey, 2002). Latent bradyzoites have the fitness to
transmutation among strain types through powerful opportunistic variability; with estimated
􀁚􀁒􀁕􀁏􀁇􀁚􀁌􀁇􀁈􀀃 􀁓􀁕􀁈􀁙􀁄􀁏􀁈􀁑􀁆􀁈􀀃 􀁒􀁉􀀃 􀁄􀁅􀁒􀁘􀁗􀀃 􀀕􀀃 􀁅􀁌􀁏􀁏􀁌􀁒􀁑􀀃 􀁓􀁈􀁒􀁓􀁏􀁈􀀃 􀁒􀁕􀀃 􀁘􀁓􀀃 􀁗􀁒􀀃 􀁄􀀃 􀁗􀁋􀁌􀁕􀁇􀀃 􀁒􀁉􀀃 􀁗􀁋􀁈􀀃 􀁚􀁒􀁕􀁏􀁇􀂶􀁖􀀃 population are
harboring the latent stage of this zoonoses, its ranks amongst the 10 most commonly latent
opportunistic pathogens, and proof the third leading cause of death among
immunocompromised individuals (Tenter et al., 2000; Kovari et al., 2010).
The pathogen has both intestinal and extra intestinal cycle only in the feline species
[final hosts], and an extra intestinal cycle in human and all intermediate hosts, including
herbivores, omnivores, and carnivores (WHO, 1979). Host infection may be vertically by
tachyzoites (acute stage) that transmitted materno-fetal series to either primary infection or
opportunity of latent stage re-conversion (bradyzoite_tachyzoite) (Bonametti et al., 1997 and
Boothroyd, 2009). While horizontal transmission through consumption oocyst contaminated
food or water, but eating undercooked meat harboring viable latent bradyzoites was definite as
a major mode of humans' toxoplasmosis (Dubey and Baetii, 1988). During unfit host immune
condition, and consequence to latent stage opportunisty, the protozoan stimulate serious
affection, and signify higher morbidity and mortality including humans with Acquired Immuno-
Deficiency Syndrome (AIDS) or submitted to corticosteroids and cancer chemotherapy (Lu et
al., 2006). Host opportunisty was confirmed sequel to reactivation of primary infection (Buxton,
1990), this the real answer for the old unanswered question of why toxoplasmosis was
diagnostic more prevalent during pregnancy and with AIDS or immune-compromised
individuals (Jones et al., 2001).
􀀊􀀕􀀐􀀌􀀎􀀍􀀛􀀚􀀅􀀉􀀃􀀐􀀂􀀅􀀐􀀂􀀌􀀅􀀓􀀕􀀜􀀇􀀏􀀅􀀂􀀘􀀓􀀕􀀎􀀅􀀃􀀊􀀊􀀎􀀌􀀄􀀄􀀝􀀅􀀞􀀊􀀄􀀇􀀚􀀅􀀉􀀃􀀊􀀊􀀅􀀋􀀌􀀅􀀒􀀃􀀄􀀍􀀘􀀄􀀄􀀅􀀐􀀂􀀌􀀅􀀕􀀒􀀟􀀕􀀎􀀍􀀌􀀄􀀅􀀃􀀎
􀀒􀀃􀀕􀀔􀀎􀀇􀀄􀀃􀀄􀀅 􀀕􀀎􀀒􀀅 􀀑􀀏􀀌􀀟􀀌􀀎􀀐􀀃􀀟􀀌􀀅 􀀍􀀇􀀎􀀐􀀏􀀇􀀊􀀅 􀀓􀀌􀀕􀀄􀀘􀀏􀀌􀀄􀀅 􀀉􀀂􀀃􀀍􀀂􀀅 􀀄􀀌􀀏􀀟􀀌􀀅 􀀏􀀌􀀒􀀘􀀍􀀃􀀎􀀔
􀀌􀀍􀀇􀀎􀀇􀀓􀀃􀀍􀀕􀀊􀀅 􀀒􀀏􀀕􀀉􀀅 􀀋􀀕􀀍􀀈􀀄􀀅 􀀇􀀎􀀅 􀀕􀀎􀀃􀀓􀀕􀀊􀀄􀀅 􀀕􀀎􀀒􀀅 􀀂􀀘􀀓􀀕􀀎􀀅 􀀂􀀌􀀕􀀊􀀐􀀂􀀝􀀅 􀀁􀀇􀀙􀀇􀀑􀀊􀀕􀀄􀀓􀀕􀀅 􀀔􀀇􀀎􀀒􀀃􀀃
􀀃􀀄􀀅 􀀕􀀎􀀅 􀀃􀀒􀀌􀀕􀀊􀀅 􀀖􀀇􀀇􀀎􀀇􀀐􀀃􀀍􀀅 􀀍􀀇􀀍􀀍􀀃􀀒􀀃􀀕􀀎􀀅 􀀑􀀏􀀇􀀐􀀇􀀖􀀇􀀕􀀎􀀠􀀅 􀀃􀀐􀀅 􀀍􀀕􀀎􀀅 􀀄􀀑􀀏􀀌􀀕􀀒􀀅 􀀟􀀃􀀕􀀅 􀀓􀀇􀀄􀀐􀀅 􀀐􀀛􀀑􀀃􀀍􀀕􀀊
􀀖􀀇􀀇􀀎􀀇􀀐􀀃􀀍􀀅􀀓􀀕􀀎􀀎􀀌􀀏􀀄􀀚􀀅 􀀋􀀛􀀅 􀀒􀀃􀀏􀀌􀀍􀀐􀀚􀀅 􀀍􀀛􀀍􀀊􀀇􀀅 􀀕􀀎􀀒􀀅 􀀄􀀕􀀑􀀏􀀇􀀖􀀇􀀇􀀎􀀇􀀄􀀃􀀄􀀝􀀅 􀀡􀀃􀀎􀀍􀀌􀀅 􀀂􀀘􀀓􀀕􀀎􀀅 􀀋􀀊􀀇􀀇􀀒
􀀐􀀏􀀕􀀎􀀄􀀗􀀘􀀄􀀃􀀇􀀎􀀅 􀀕􀀎􀀒􀀅 􀀇􀀏􀀔􀀕􀀎􀀄􀀅 􀀐􀀏􀀕􀀎􀀄􀀑􀀊􀀕􀀎􀀐􀀕􀀐􀀃􀀇􀀎􀀅 􀀉􀀌􀀏􀀌􀀅 􀀓􀀌􀀒􀀃􀀍􀀃􀀎􀀕􀀊􀀅 􀀕􀀒􀀟􀀕􀀎􀀍􀀌􀀒􀀚􀀅 􀀊􀀕􀀐􀀌􀀎􀀍􀀛
􀀃􀀎􀀅 􀀐􀀇􀀙􀀇􀀑􀀊􀀕􀀄􀀓􀀇􀀄􀀃􀀄􀀅 􀀂􀀕􀀄􀀅 􀀇􀀍􀀍􀀘􀀑􀀃􀀌􀀒􀀅 􀀄􀀘􀀑􀀌􀀏􀀃􀀇􀀏􀀅 􀀂􀀌􀀕􀀊􀀐􀀂􀀅 􀀕􀀐􀀐􀀌􀀎􀀐􀀃􀀇􀀎􀀅 􀀕􀀓􀀇􀀎􀀔􀀄􀀐􀀅 􀀊􀀕􀀐􀀌􀀎􀀐
􀀇􀀑􀀑􀀇􀀏􀀐􀀘􀀎􀀃􀀄􀀐􀀃􀀍􀀅 􀀑􀀕􀀐􀀂􀀇􀀔􀀌􀀎􀀄􀀝􀀅 􀀢􀀕􀀐􀀌􀀎􀀍􀀛􀀅 􀀃􀀎􀀅 􀀐􀀇􀀙􀀇􀀑􀀊􀀕􀀄􀀓􀀇􀀄􀀃􀀄􀀅 􀀃􀀄􀀅 􀀕􀀎􀀅 􀀌􀀎􀀍􀀛􀀄􀀐􀀓􀀌􀀎􀀐
􀀄􀀌􀀍􀀘􀀏􀀃􀀎􀀔􀀅 􀀒􀀇􀀏􀀓􀀕􀀎􀀐􀀅 􀀋􀀏􀀕􀀒􀀛􀀖􀀇􀀃􀀐􀀌􀀄􀀅 􀀐􀀇􀀅 􀀑􀀌􀀏􀀄􀀃􀀄􀀐􀀅 􀀕􀀎􀀒􀀅 􀀄􀀑􀀏􀀌􀀕􀀒􀀅 􀀉􀀃􀀐􀀂􀀃􀀎􀀅 􀀎􀀌􀀉􀀅 􀀂􀀇􀀄􀀐􀀄
􀀉􀀃􀀐􀀂􀀇􀀘􀀐􀀅 􀀇􀀋􀀊􀀃􀀔􀀕􀀐􀀌􀀅 􀀏􀀌􀀣􀀘􀀃􀀏􀀌􀀅 􀀐􀀇􀀅 􀀑􀀏􀀇􀀍􀀌􀀌􀀒􀀃􀀎􀀔􀀅 􀀐􀀂􀀏􀀇􀀘􀀔􀀂􀀅 􀀃􀀐􀀄􀀅 􀀄􀀌􀀙􀀘􀀕􀀊􀀅 􀀄􀀐􀀕􀀔􀀌􀀝􀀅 􀀤􀀘􀀓􀀕􀀎􀀄􀀚
􀀕􀀊􀀊􀀅 􀀉􀀕􀀏􀀓􀀅 􀀋􀀊􀀇􀀇􀀒􀀌􀀒􀀅 􀀓􀀕􀀓􀀓􀀕􀀊􀀄􀀅 􀀕􀀎􀀒􀀅 􀀋􀀃􀀏􀀒􀀄􀀅 􀀕􀀏􀀌􀀅 􀀃􀀎􀀐􀀌􀀏􀀓􀀌􀀒􀀃􀀕􀀐􀀌􀀅 􀀂􀀇􀀄􀀐􀀄􀀅 􀀂􀀕􀀏􀀋􀀇􀀏􀀃􀀎􀀔
􀀐􀀃􀀄􀀄􀀘􀀌􀀅 􀀍􀀛􀀄􀀐􀀄􀀠􀀅 􀀉􀀂􀀃􀀊􀀌􀀅 􀀇􀀇􀀍􀀛􀀄􀀐􀀄􀀅 􀀄􀀂􀀌􀀒􀀒􀀌􀀏􀀅 􀀈􀀃􀀐􀀐􀀌􀀎􀀄􀀅 􀀕􀀎􀀒􀀅 􀀏􀀌􀀥􀀄􀀂􀀌􀀒􀀒􀀌􀀏􀀅 􀀊􀀕􀀐􀀌􀀎􀀐􀀅 􀀃􀀎􀀗􀀌􀀍􀀐􀀌􀀒
􀀍􀀕􀀐􀀄􀀅 􀀕􀀏􀀌􀀅 􀀐􀀂􀀌􀀅 􀀇􀀎􀀊􀀛􀀅 􀀒􀀌􀀗􀀃􀀎􀀃􀀐􀀃􀀟􀀌􀀅 􀀂􀀇􀀄􀀐􀀚􀀅 􀀐􀀂􀀌􀀛􀀅 􀀕􀀏􀀌􀀅 􀀇􀀗􀀅 􀀑􀀘􀀋􀀊􀀃􀀍􀀅 􀀂􀀌􀀕􀀊􀀐􀀂􀀅 􀀃􀀓􀀑􀀕􀀍􀀐􀀅 􀀐􀀂􀀏􀀇􀀘􀀔􀀂
􀀐􀀂􀀌􀀓􀀅 􀀕􀀋􀀊􀀌􀀅 􀀐􀀇􀀅 􀀏􀀌􀀔􀀘􀀊􀀕􀀏􀀅 􀀑􀀇􀀊􀀊􀀘􀀐􀀌􀀅 􀀐􀀂􀀌􀀅 􀀄􀀘􀀏􀀏􀀇􀀘􀀎􀀒􀀃􀀎􀀔􀀅 􀀋􀀛􀀅 􀀇􀀇􀀍􀀛􀀄􀀐􀀄􀀅 􀀓􀀇􀀏􀀌􀀅 􀀐􀀂􀀕􀀎􀀅 􀀇􀀎􀀍􀀌
􀀒􀀘􀀏􀀃􀀎􀀔􀀅􀀐􀀂􀀌􀀃􀀏􀀅􀀊􀀃􀀗􀀌􀀅􀀄􀀑􀀕􀀎􀀝

Advanced epidemiological studies on toxoplasmosis in animals and man

Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular To... more Toxoplasmosis is one of the most common parasitic zoonoses world-wide, caused by intracellular Toxoplasma gondii protozoan capable of infecting all types of nucleated mammalian host cells. The disease is of economic veterinary importance and of public health hazard in human side. In the present study, antigens were successfully prepared from RH strain tachyzoites and were used for sero-diagnosis of random samples of both human& buffaloes by ELISA and SFDT. Where, the percent of sero-positive was [56.2&50] and [14.6&10.1] respectively. These results were compared with the commercial LAT that gave uncorrelated results and judged as unsuitable test for diagnostic purposes. New techniques were used for developing the performance of maintenance and preservation of RH strain tachyzoites. A modified method for long term acute stage in mice was used for the first time and succeeds to harboring the T. gondii protozoan up to 12 DPI. Also, it was proven that the liquid nitrogen preservation is the favorable media to save the RH strain tachyzoites viable for more than 18 months, also, DMSO media at (-70ºC) save the tachyzoites viable for about one year. The sero-prevalence of naturally infected Egyptian kittens was [41.8] by using SFDT where, the shedding percent recorded was [3.6 ], and experimental infection of sero-negative kittens was done to detect the average time of both prepatent period [5-7days] & the shedding course [12days ]. The sero-positive shedder cats were re-housed at rest period for 2months before the first re-shed trial followed by 3 months before the second trial where cats re-infected with the T. gondii RH strain bradyzoites accompanied with and without corticosteroid injection corresponding to the two trials where the result obtained was [7.7] in corticosteroid non-treated group and [41.7] in corticosteroid treated one. The re-shedding investigation is absolutely the first Egyptian trial. Also, in the present study, another two new developed Egyptian experiments were successfully done for the first time. The first was in vivo induction of RH strain bradyzoites in mice brain and the isolation at 45 DPI.also, the success for reactivation this chronic stage to ensure the parasite opportunisty. Where, latent infection was confirmed in 100% of corticosteroid treated mice. In the second experiment, T. gondii DNA was isolated from some Egyptian kinds of processed meat; basterma, showerma and cooked brain. Where, the total percent of isolation was [14.4]. Mice brain emulsion containing bradyzoites were used as processed meat model and exposed to different types of treatments: heating, chilling, freezing and salting. The total percentage of bradyzoites persistence with different types of treatments was recorded and heating is found to be effective than salting as a method of treatment while freezing is more effective than chilling as a storage method of processed meat. The main conclusions: [1] cats re-shed oocysts under certain circumstances and could contaminate the surrounding environment more than one time [2] processed meat was confirmed as non-neglected source of human toxoplasmosis [3] latent toxoplasmosis is considered a potential mode of Advanced epidemiological studies on toxoplasmosis in animals and man. Abstract infection maximizing the host risk and must be statistically estimated compared to acquired and congenital toxoplasmosis.

Hassan Elfadaly أسم الله على مايذبح

يسألونك ماذا أحل لهم، قل أحل لكم الطيبات ) (المائدة 4 ) جعل الله سبحانة وتعالى الذبح أحد أهم مناس... more يسألونك ماذا أحل لهم، قل أحل لكم الطيبات ) (المائدة 4 ) جعل الله سبحانة وتعالى الذبح أحد أهم مناسك الفرائض و السن. لذلك لم يكن الذبح فى الاسلام مقصودا بة أكل لحم طهورا طيبا فقط , بل هى أحد المناسك الدينية التى يتقرب بها المسلم لله عز وجل. لهذا شرعت بضوابت تضمن تحقيق أهداف دينية من خلال لحم طيبا صحيا مباركا فية , يضمن سلوكا يتناسب مع صفات وأنسانية المسلم, ويحافظ على رحمتنا بالحيوان.لذلك حرص الله عز وجل على طيب طعام المسلم وان يكون عونا لة لتحقيق ما يريدة الله سبحانة وتعالى لنا من حسن الخلق وجميل الطباع والتى تؤهلنا للقيام بالمناسك الدينية ونحن فى أرقى المشاعر الأنسانية,ويأتى فرض ذكر اسم اللة على مايذبح واستقبال القبلة تأكيدا لقدسية تلك الشريعة ,فذكر اسم الله أثناء الذبح يطمئن قلب المسلم (الا بذكر الله تطمئن القلوب) فيبعد عنة الأثار السلبية لهذة العملية التى تتسم بالعنف ويذهب عنة وحشيتها فيتحلا بهدؤة فلاتثيرة الدماء ولاتذيدة الا طمئنينة وخشوع لرب العالمين و لذلك حدد لنا الله سبحانة وتعالى شروط للذبيحة و لعملية الذبح وشرعيتها وإن كل عمل مخالف لأحكام الشريعة الإسلامية وفقا لما جاء في الكتاب والسنة سينتج عنه ضرر ومرض وسوء عاقبة.

أسم الله على مايذبح

والله سبحانه وتعالى أمرنا بالتسمية عند الذبح فقال جل جلاله فى سورة ‏الأنعام

( فكلوا مما ذكر اسم الله عليه ان كنتم بآياته مؤمنين)( آية 18 )
وقال جل ‏‏شأنه { ولا تأكلوا مما لم يذكر اسم الله عليه وإنه لفسق} (آية 121) .
وقال أيضا ( أنعام لايذكرون اسم الله عليها افتراء عليه ) (آية138).

إن ذكر اسم الله أثناء الذبح يطمئن قلب المسلم (الا بذكر الله تطمئن القلوب) فيبعد عنة الأثار السلبية لهذة العملية التى تتسم بالعنف ويذهب عنة وحشيتها ويجعل منها منسك دينى يتقرب بة المسلم لله عز وجل فيتحلا بهدؤة فلاتثيرة الدماء ولاتذيدة الا طمئنينة وخشوع لرب العالمين. أنّ رَسُولِ اللَّهِ -صلى الله عليه وسلم- قَالَ «لايذبحن احدكم حتى يصلى», ولاعجب من سلوك الأنعام إن ذكر اسم الله عليها فأن للأنعام قلوب أرتضت منذ الخلق ماكلفت بة, لذلك تجد الدابة التى يذكر اسم الله عليها مستسلمه لأمر ربها,فرحة بما أتاها من فضل وتكريم وجعل دمائها الطاهرة تسيل راضية بذكر الله.

إن إهمال ذكر اسم الله على الذبيحة يجعل من لحمها بؤرة تغص بالدماء سابحةً فيه الجراثيم، كما أن بقاء الدم في لحوم الذبائح التي لم يذكر اسم الله عليها يؤدي إلى تبقعها وفساد مظهرها مما يجعلها تفقد قيمتها التجارية، بالإضافة إلى سرعة فسادها عند التخزين مما يُلحق الضرر الاقتصادي بالمجتمع. لقد كانت كل الفحوص الدموية والنسيجية كلها لمصلحة التكبير على اللحم. فاللحم الذي كبر عليه يكاد يكون طاهراً نظيفاً من كل تلوث ومن كل أثر للبقع الدموية والجراثيم والمستعمرات الجرثومية. أما اللحم غير المكبر عليه نجد أن الفحوص الدموية والنسيجية والصور التي عرضت بهذا الموضوع كانت موبوءة وملوثة بشكل كبير جدا ًلا يمكن إهماله ولا يمكن التغاضي عنه.
يقول الدكتور أنس الناظر:عندما كنت في الخارج أثناء دراستي رأيت كيف يتماثل المرضى تماماً للشفاء من الكثير من الأمراض المعديه عند امتناعهم عن تناول اللحوم, كان تعليلي الأساسي لهذه الحالة هو أن هذه اللحوم غير مذبوحة على الطريقة الإسلامية، إما مخنوقة خنقاً أو مقتولة ثم مذبوحة، ولكن بعدما تم اكتشاف أن للتكبير هذه الأهمية الكبيرة في منع بقاء الدم ضمن الذبيحة وبالتالي منع التكاثر الجرثومي ضمن الذبيحة تبين لي تماماً أن هذا الموضوع ليس عن عبث أبداً. فالامتناع عن تناول اللحوم كتلك اللحوم التي لم يكبر عليها والتي تم التكاثر الجرثومي فيها لهذه الدرجة التي تحتوي الزيفان والانزيمات المفرزة من قبل الجراثيم هي بالتأكيد لحوم مسببة للمرض ليست شافية منه.

Hassan Elfadaly الحم الطيب

جعل الله سبحانة وتعالى الذبح أحد أهم مناسك الفرائض و السنن. لذلك لم يكن الذبح فى الاسلام مقصودا ب... more جعل الله سبحانة وتعالى الذبح أحد أهم مناسك الفرائض و السنن. لذلك لم يكن الذبح فى الاسلام مقصودا بة أكل لحم طهورا طيبا فقط , بل هى أحد المناسك الدينية التى يتقرب بها المسلم لله عز وجل. لهذا شرعت بضوابت تضمن تحقيق أهداف دينية من خلال لحم طيبا صحيا مباركا فية , يضمن سلوكا يتناسب مع صفات وأنسانية المسلم, ويحافظ على رحمتنا بالحيوان.لذلك حرص الله عز وجل على طيب طعام المسلم وان يكون عونا لة لتحقيق ما يريدة الله سبحانة وتعالى لنا من حسن الخلق وجميل الطباع والتى تؤهلنا للقيام بالمناسك الدينية ونحن فى أرقى المشاعر الأنسانية,ويأتى فرض ذكر اسم اللة على مايذبح واستقبال القبلة تأكيدا لقدسية تلك الشريعة ,فذكر اسم الله أثناء الذبح يطمئن قلب المسلم (الا بذكر الله تطمئن القلوب) فيبعد عنة الأثار السلبية لهذة العملية التى تتسم بالعنف ويذهب عنة وحشيتها فيتحلا بهدؤة فلاتثيرة الدماء ولاتذيدة الا طمئنينة وخشوع لرب العالمين و لذلك حدد لنا الله سبحانة وتعالى شروط للذبيحة و لعملية الذبح وشرعيتها وإن كل عمل مخالف لأحكام الشريعة الإسلامية وفقا لما جاء في الكتاب والسنة سينتج عنه ضرر ومرض وسوء عاقبة.

Hassan Elfadaly تكريم الأستاذ الدكتور/ محى الدين عبد الحفيظ

من أين ابدأ فى مديح أستاذى فما الشعر ينصفة ولا الأقوال ينصفة رب العالمين بعلمة ... more من أين ابدأ فى مديح أستاذى فما الشعر ينصفة ولا الأقوال
ينصفة رب العالمين بعلمة ويجزية عنا خير جزاء
محى الدين بذل للعلم لايبتغى جاها ولامال

All cases have been linked through travel to or residence in Saudi Arabia, Qatar, Jordan, and Uni... more

Hassan Elfadaly تكريم الأستاذ الدكتور/ أحمد جعفر حجازى

ناديت الابيات تكرمك فجاء اسمك للأبيات تكريما احب النحل فأورثتة خصالها فأخلاقة عسل مصفى كا... more

Master Degree of Vet. Sci. (zoonosis), Faculty of Vet. Med., Cairo Univ.,, 2002, Cairo University. Epidemiological studies on toxoplasmosis in at risk sheep, goats and women.

Hassan Elfadaly دكتور حسن القضالى والصحة العامة

دكتور حسن القضالى والصحة العامة دكتور حسن القضالى والصحة العامة