Papers by Gustavo Carneiro
Research, Society and Development, 2021
Este estudo avaliou a influência do estresse térmico na resposta superovulatória e no perfil bioq... more Este estudo avaliou a influência do estresse térmico na resposta superovulatória e no perfil bioquímico de ovelhas Dorper superovuladas em ambiente tropical semiárido. Foram utilizadas 13 ovelhas da raça Dorper, as quais foram submetidas à sincronização do estro e ao tratamento superovulatório, em dois períodos diferentes [termoneutro (TN) e estresse térmico (ET)]. As ovelhas foram inseminadas por laparoscopia utilizando sêmen congelado 42-43 horas após a remoção do dispositivo intravaginal. Cinco dias após a inseminação artificial, foi realizada laparotomia para avaliação da resposta ovariana e lavagem uterina. Coletou-se sangue para avaliação das variações nas concentrações séricas de glicose, frutosamina, triglicerídeos, β-hidroxibutirato (BHB), creatinina, ureia, proteína total, albumina, AST/TGO, GGT, Fosfatase Alcalina, Cálcio, Fosfato e Magnésio. Os períodos termoneutro (TN) e de estresse térmico (ET) tiveram temperaturas médias de 22,01 °C e 27,61 °C, respectivamente, com um...
Revista de Ciências Agroveterinárias, 2015
Objetivou-se com o presente trabalho avaliar a expressao das caracteristicas comportamentais de e... more Objetivou-se com o presente trabalho avaliar a expressao das caracteristicas comportamentais de eguas em periodo de estro e diestro, estabuladas submetidas a tecnicas de inseminacao artificial (IA) e transferencia de embrioes (TE) em uma clinica de reproducao equina. Utilizou-se 16 eguas doadoras das racas Mangalarga Marchador, Quarto de Milha e Campolina, com idades entre tres e 12 anos e pesos entre 490 a 740 kg. Essas foram estabuladas em baias individuais de dimensao 4 x 4 metros, tendo acesso a um piquete de exercicio durante algumas horas do dia. As eguas foram observadas por um periodo de 24 horas, anotando a cada dez minutos as atividades e o tempo gasto por cada animal nessas atividades, nas fases de estro e diestro, analisando os parâmetros comportamentais de: frequencia de miccao (MIC), frequencia de defecacao (DEF), tempo em ocio (O), tempo gasto com se alimentando (ALI), frequencia de consumo de agua (H 2 O), tempo caminhando (CAM), tempo dormindo em pe (DP), tempo dorm...
The presence of twinning has long been recognized as one of the most important causes of reproduc... more The presence of twinning has long been recognized as one of the most important causes of reproductive wastage in the mare. The incidence of abortion due to twinning has declined subsequent to the routine use of ultrasonography for pregnancy diagnosis. The early and accurate diagnosis and management of twins remains an important part of routine equine breeding management. While options exist for the management of twins postfixation, more mares will give birth to a single foal if one of the vesicles is eliminated during the mobility phase. The early and accurate diagnosis for handling twins is of great importance in equine reproductive management.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Oct 1, 2017
Estudo da vascularização folicular e do corpo lúteo de éguas cíclicas tratadas com extrato de pit... more Estudo da vascularização folicular e do corpo lúteo de éguas cíclicas tratadas com extrato de pituitária equina utilizando ultrassom Doppler colorido [Study of follicular and corpus luteum vascularization in mares treated with Equine Pituitary Extract using ultrasound color Doppler]
Journal of Equine Veterinary Science, 2018
Due to the mechanization in agriculture and in transports, the number of donkeys reared in Europe... more Due to the mechanization in agriculture and in transports, the number of donkeys reared in Europe was reduced of 50% in the last 20 years and more than 80% since the middle of the last century. During this period, no attention was devoted to the breeding selection and biodiversity conservation, with the results that, nowadays, most of European breeds of donkey are threated of extinction. The remaining donkey, in Europe, are employed for niche purposes as mule production or maintenance of soil and landscape. The consumption of donkey meat is not popular in Europe and a few animals are sent to the slaughterhouse. A new interest is growing in Europe for donkey milk, mostly for human consumption, but also for the production of beauty products. Marginal areas of donkey employment are the social and leisure uses. In spite the fact that interest for donkey in Europe is very marginal, and that very few or even no funding for research were provided from national or European authorities, more or less one third of paper published on different aspect of donkey sciences in the last 20 years where produced in Europe.
Investigação Científica e Técnica em Ciência Animal 2, 2019
Revisão: Os Autores O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade s... more Revisão: Os Autores O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.
Revista Brasileira de Reprodução Animal, 2021
A endometrite é a principal causa de infertilidade em éguas, além de gerar grandes transtornos ec... more A endometrite é a principal causa de infertilidade em éguas, além de gerar grandes transtornos econômicos e produtivos. Objetivou-se com esse trabalho, realizar uma revisão sistemática sobre endometrite equina, sua etiologia, os mecanismos próprios de defesa uterina mecânicos e quimiotáticos. Avaliando ainda os principais microrganismos envolvidos no processo bem como sua virulência e resistência antimicrobiana. Apresentamos ainda uma proposta de abordagem diagnóstica contemplando a identificação dos agentes, susceptibilidade farmacológica associadas a comprovação da produção de biofilme, assim como sua graduação, possibilitando assim em caso de comprovação de produção de biofilme uma terapia alternativa eficaz para a quebra desse biofilme associado a um tratamento antibacteriano específico. A correlação desses testes possivelmente fornecerá subsídios ainda mais importantes para melhorar a eficiência reprodutiva dos rebanhos equinos.
Animal Reproduction, 2021
The objective of this study was to evaluate the effects of the addition of different concentratio... more The objective of this study was to evaluate the effects of the addition of different concentrations of ozone to quarter horse semen submitted to cryopreservation. Six ejaculates from four stallions were collected and were divided in four experimental groups: a control group (BotuCRIO® extender) and three other groups with BotuCRIO® ozonized at concentrations of 6, 8 and 12 μg of O 3 /mL. The semen samples were diluted (200 x 10 6 spermatozoa/mL), filled in straws and frozen. After thawing (37 ºC, 30s), the samples were evaluated at 0, 30 and 60 minutes of incubation regarding sperm kinetics by a computer-assisted sperm analysis (CASA), and plasma membrane integrity (PMI), acrosome integrity (ACi) and mitochondrial membrane potential (MMP) by fluorescent probes. There was a reduction in the kinetic parameters total motility (TM), progressive motility (PM), curvilinear velocity (VCL), straight line velocity (VSL) and average path velocity (VAP) in all groups during the thermoresistance test (TT), a pattern also found in PMI and MMP analyses (p<0.05). There was no difference (p>0.05) between the control and treatment (6, 8, and 12 μg of O 3 /mL) groups, in any of the evaluated times for the kinetic parameters TM, linearity (LIN), straightness (STR), wobble index (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF). Regarding the VCL, VSL and VAP parameters, the group treated with 6 μg did not differ from the control or from 8 μg, but was higher than 12 μg at 30 and 60 minutes. ACi and PMI did not differ between groups (p>0.05), but PMI was lower in groups 8 μg and 12 μg compared to the control and 6 μg (p<0.05). It was concluded that the addition of ozone does not present beneficial effects for cryopreservation of equine semen at the concentrations used and decreases important parameters of fertility.
Biopreservation and Biobanking, 2020
l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through b-oxidatio... more l-Carnitine (LC) plays a key role in sperm metabolism, easily providing energy through b-oxidation, which positively affects motility. The objective of this study was to investigate the association between blood plasma and seminal plasma LC levels, as well as the effect of LC as an additive in a skimmed milk-based extender during sperm storage at 5°C. In the first experiment, semen and blood samples from 14 Quarter Horse stallions were used. The LC content in blood plasma and seminal plasma was determined by spectrophotometry and their relationships with seminal parameters were evaluated. In the second experiment, ejaculates (n = 16) from four Quarter Horses were used. Each ejaculate was split into four treatment groups with different LC concentrations: 0 (control), 0.5, 1.0, and 2.0 mM. Sperm motility, integrity of plasma and acrosomal membranes, intracellular reactive oxygen species content, and plasma membrane stability were evaluated immediately after samples reached 5°C (0 hour) and after 24, 48, and 72 hours. There was a positive correlation (p < 0.05) between LC levels in seminal plasma with both sperm concentration and plasma and acrosomal membrane integrity. Furthermore, the addition of LC (1 and 2 mM) preserved the motility of equine sperm stored at 5°C. It was concluded that the concentrations of LC with seminal plasma present correlate to semen parameters and the addition of LC to skimmed milk-based extender preserves the motility of equine sperm stored at 5°C for up to 48 hours.
The goat business is in expansion, particularly in developing countries. To reach this goal, it i... more The goat business is in expansion, particularly in developing countries. To reach this goal, it is necessary an organization of the agribusiness. The easy adaptation to unfavorable weather associated to low cholesterol profile in the goat meet makes this segment an economic atraction for the agribusiness in semi-arid areas. The increase in productivity demands the use of biotechnology. The popularization of byotechniques such as estrous synchronization, artificial insemination (AI) with fresh or frozen semen and Embryo Trasnfer (ET) can offer this genetic improvement in a short time. The embryo collection and transfer using the transcervical technique, without necessity of surgery apparatus, gives an opportunity to generate a genetic gain by a noninvasive methodology with good results. The use of these techniques can accelerate the production and technological advances in this specie with a low budget, increasing therefore the productivity and the development of unfavorable regions.
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2019
The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine o... more The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated shortly after follicular aspiration, 79.00% were in GV, GVBD, MI, while 13.40%, were in MII and 7.60%, D/NI. Significant difference was observed in different concentrations (T100, T200 and T150µM) in oocytes that have reached the MII phase comp...
Ciência Animal Brasileira, 2010
Molecular Reproduction and Development, 2003
The isomer 9‐cis of retinoic acid (9‐cis‐RA) exerts a beneficial effect on bovine in vitro develo... more The isomer 9‐cis of retinoic acid (9‐cis‐RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9‐cis‐RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus–oocyte complexes (COCs) were treated with the found physiological dose 9‐cis‐RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF‐I, by reverse transcriptase‐polymerase chain reaction (RT‐PCR), in cumulus‐granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9‐cis‐RA, but not by 1% ethanol (vehicle). However, we did not detect IGF‐I expression, both in dependence on or in the absence of 9‐cis‐RA acting on cumulus‐granulosa cells. The ability of vitrified blastocysts to survive in vitro was not impro...
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
Foram utilizados ejaculados (n=25) de garanhões para avaliar o efeito de glutationa peroxidase (G... more Foram utilizados ejaculados (n=25) de garanhões para avaliar o efeito de glutationa peroxidase (GPx) e cisteína na viabilidade de espermatozoides congelados. O sêmen foi diluído em Botu Crio, com antioxidantes, e foram formados os grupos: G1, Controle; G2, 1U GPx ; G3, 5U GPx; G4, 0,5mM cisteína; G5, 1mM cisteína. Depois foi envasado em palhetas (0,5mL) e congelado. Após descongelação, 37°C por 30 segundos, alíquotas foram analisadas quanto à integridade de membrana plasmática (IMP) e acrossoma (IAc), potencial de membrana mitocondrial (PMM) e cinética, nos tempos zero (T0) e 60 minutos (T60). GPx 5U e cisteína 0,5mM determinaram maior (P<0,05) IAc em T0 do que em T60. Cisteína 1mM resultou em maior (P<0,05) IAc em T60 do que GPx 1 e 5U e cisteína 0,5mM. O PMM de um garanhão no T60 foi mais alto (P<0,05) do que o de dois garanhões. VCL e VAP foram maiores (P<0,05) no T0 do que no T60 do grupo controle, e um garanhão apresentou, em geral, valores cinéticos mais altos (P&l...
Animal, 2013
In horses, successfulin vitrofertilization procedures are limited by our inability to consistentl... more In horses, successfulin vitrofertilization procedures are limited by our inability to consistently mature equine oocytes byin vitromethods. Growth hormone (GH) is an important regulator of female reproduction in mammals, playing an important role in ovarian function, follicular growth and steroidogenesis. The objectives of this research were to investigate: the effects of equine growth hormone (eGH) and insulin-like growth factor-I (IGF-I) on thein vitromaturation (IVM) of equine oocytes, and the effects of eGH in addition to estradiol (E2), gonadotropins (FSH and LH) and fetal calf serum (FCS) on IVM. We also evaluated the cytoskeleton organization of equine oocytes after IVM with eGH. Equine oocytes were aspirated from follicles <30 mm in diameter and matured for 30 h at 38.5°C in air with 5% CO2. In experiment 1, selected cumulus–oocyte complexes (COCs) were randomly allocated as follows: (a) control (no additives); (b) 400 ng/ml eGH; (c) 200 ng/ml IGF-I; (d) eGH + IGF-I; and ...
Revista Brasileira de Reprodução Animal, 2021
The objective of this study was to evaluate the main microorganisms isolated from the uterus of s... more The objective of this study was to evaluate the main microorganisms isolated from the uterus of subfertile mares and the resistance profile to antimicrobial agents in vitro of the bacteria. Samples were collected from 41 mares with a history of subfertility. For bacterial culture the samples were stored in tubes containing BHI broth and for fungal culture containing 0.9% NaCl solution and sent to LABRAPE-UFRPE. The isolated bacteria were subjected to the in vitro sensitivity test for Amikacin, Gentamicin, Enrofloxacin, Azithromycin, Ceftriaxone, Tetracycline, Penicillin and Ampicillin. 75.6% of the samples presented microbiological isolation, being 25.8% Staphylococcus spp.; 12.9% nonlactose-fermenting Gram-negative bacilli; 3.22% β-hemolytic streptococcus; 3.22% E. coli; 3.22% Klebsiella spp. and 3.22% Proteus spp.; 22.6% Bacillus spp. and 6.45% Micrococcus spp. Among the isolated samples for fungi 6.45% were Cladosporium spp. It was observed that 12.9% of the animals had mixed inf...
Research, Society and Development, 2021
Objetivou-se com este estudo observar a eficácia da utilização das Proteínas Associadas à Gestaçã... more Objetivou-se com este estudo observar a eficácia da utilização das Proteínas Associadas à Gestação (PAG´s) como método de diagnóstico precoce de gestação em vacas leiteiras da raça Girolando. Por meio de um teste de reação colorimétrica, recomendado para uso em vacas a partir do 28º dia pós-serviço, o VISUAL-PAG do “IDEXX Laboratories”. Verificou-se a sensibilidade e especificidade do teste aos 25, 26, 27 e 28 dias pós-Inseminação Artificial em Tempo Fixo (IATF), utilizando-se 57 fêmeas multíparas, submetidas a quatro coletas sequenciais de amostras de sangue nos dias descritos. O material foi coletado através de venopunção dos vasos coccígenos em tubos de vácuo sem anticoagulante, processado e armazenado a -20ºC para posterior análise. Aos 32 dias da IATF todas as fêmeas foram submetidas ao diagnóstico de gestação por ultrassonografia (US), teste padrão do experimento. Os resultados foram obtidos através de um estudo de concordância, onde revelou sensibilidade de 100% e especificid...
Endometritis is the main cause of infertility in mares, besides generating major economic and pro... more Endometritis is the main cause of infertility in mares, besides generating major economic and productive disorders. The objective was to characterize the cytology, microbiological and fungal culture, as well as the identification of biofilm formation in mares with endometritis. Samples of seventy mares were collected for uterine cytology and microbiological examination. The determination of biofilm formation was performed using violet crystal technique. Of the seventy mares diagnosed with endometritis 43 (61.4%) presented bacterial and/or fungal infection. From the 43 mares, 30.2% (13/43) presented fungal infection, the most evidenced were Aspergillus spp. and Candida albicans, Curvalaria spp., Cladosporium spp. and Zygomices spp. Of the identified fungi, 9/13 (69.2%) formed biofilm. These results reveal above average numbers of fungal infection compared to the literature. Several causes are related to the increase in cases of fungal endometritis, among them the increase in the use ...
Semina: Ciências Agrárias, 2020
Equine alphaherpesvirus is a major pathogen that causes respiratory disorders, abortion, neurolog... more Equine alphaherpesvirus is a major pathogen that causes respiratory disorders, abortion, neurological syndromes, and perinatal mortality in horse populations worldwide. The aim of this study was to determine whether the occurrence of anti-alphaherpesvirus antibodies is correlated to reproductive abnormalities in horses in the state of Pernambuco, Brazil. The virus neutralization was carried out in 322 serological samples from unvaccinated horses from 42 farms located in 16 municipalities in the Microregion of the Vale do Ipojuca, Pernambuco, Brazil. Of these horses, 23.3% (75/322; 95% CI: 18.9-28.4%) were found to be reactive to anti-alphaherpesvirus antibodies. In addition, 61.9% (26/42) of farms had at least one reactive animal, with an inter-herd variation of 5% to 100%. With regard to reproductive rates, herpetic infection was associated with the use of biological reproductive techniques such as artificial insemination (AI) and embryo transfer (ET), which uses natural mating (p ...
Reproduction, 2004
Changes in placental development have been associated with foetal abnormalities afterin vitroembr... more Changes in placental development have been associated with foetal abnormalities afterin vitroembryo manipulations. This study was designed to investigate bovine conceptus development and substrate levels in plasma and fluids inin vivo- andin vitro-produced (IVP) concepti and neonates.In vivo-produced and IVP embryos were derived by established embryo production procedures. Pregnant animals from both groups were slaughtered on days 90 or 180 of gestation, or allowed to go to term. Conceptus and neonatal physical traits were recorded; foetal, maternal and neonatal blood, and foetal fluids were collected for the determination of blood and fluid chemistry, and glucose, fructose and lactate concentrations. Placental transcripts for specific glucose transporters were determined by quantitative RT-PCR. No significant differences in uterine and conceptus traits were observed between groups on day 90. On day 180, larger uterine, placental and foetal weights, and an increase in placental gros...
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Papers by Gustavo Carneiro