A new fiberized fluorescence imaging probe is presented. This device can potentially be used for ... more A new fiberized fluorescence imaging probe is presented. This device can potentially be used for a wide range of biological or medical applications. By exploiting the chromatic aberrations of gradient index lenses, the excitation blue or near-UV excitation light is focused on the sample surface, while the red fluorescence signal is efficiently launched back to collecting fibers. The excitation fiber is single mode at the working wavelength so that a resolution of 5 m is obtained over a scanning area of several square millimeters. Experimental fluorescence images are presented. They concern either selffabricated fluorescent microsamples or views of leaves that constitute an example of biological tissues analysis. The probe can also be adapted for spectroscopic investigations.
Journal of Engineering Mechanics-asce - J ENG MECH-ASCE, 1997
The precision, accuracy, coordination, and dexterity in hand strength are seen as adequate aspect... more The precision, accuracy, coordination, and dexterity in hand strength are seen as adequate aspects to determine an individual's status and progress. In an attempt to explore those aspects, a cylindrical bar design-based measurement system has been developed. Individual isometric exertions are measured, given visual reference and feedback of the exertion levels using the computer screen display. When processed, the resulted data bring out some parameters in terms of which the aforementioned dynamic aspects can be described. This paper describes this assessment, as well as the hardware, the computer software that constitutes the system, and the calibration method. The test, protocols, and future experiences are stated. The present evaluation could serve as a baseline for diagnosis and assessments in different fields such as surgical procedures evaluation or basketball players hand assessment.
The current work describes the elaboration of a simple, sensitive and reliable -casein modified ... more The current work describes the elaboration of a simple, sensitive and reliable -casein modified glass beads, for the detection and quantification of its specific antibody anti -casein. This is an elementary receptor without electronic part, developed by grafting glass bead surface with the antigenic -casein via 3-aminopropyltriethoxy silane and then glutaraldehyde as cross-linker. The whole is realized by a classical process, called in two steps and in mild conditions where chemical protocol is optimized for -casein use. The detection and quantification of the specific reaction antibody-receptor is carried out by the technique of the second antibody labeled with horse radish peroxidase (HRP). Our receptor can detect the -casein antibody present in the serum at dilutions up to a factor 10 7 in strong ionic strength medium. The same antibody of the same serum and in the same conditions can be detected by ELISA test at dilutions up to a factor 10 5. The whole test, after our receptor realization, takes about 5 h.
... et de Mécanique de Précision, Université Ferhat Abbas [Applied Optics Laboratory, Institute o... more ... et de Mécanique de Précision, Université Ferhat Abbas [Applied Optics Laboratory, Institute of Optics and Precision Mechanics, Ferhat Abbas University ... Optics (Brooklyn, NY: Polytechnic Press) pp 33347 [15] Sumida M and Takemoto K 1984 Lens coupling of laser diodes to ...
... e Laboratoire d'Optique PM Duffieux, University of Franche-Comté, 16 Route d... more ... e Laboratoire d'Optique PM Duffieux, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France. Received 2 November 2006; revised 8 March 2007; accepted 20 April 2007. ... Thus, poly-l-lysine, fibronectin or laminin are the most commonly used substrates. ...
Optics InfoBase is the Optical Society's online library for flagship journals, partnered and... more Optics InfoBase is the Optical Society's online library for flagship journals, partnered and copublished journals, and recent proceedings from OSA conferences.
... Auteur(s) / Author(s). VIENNET C. ; ARMBRUSTER V. ; GABIOT AC ; GHARBI T. ; BRIDE J. ; HUMBER... more ... Auteur(s) / Author(s). VIENNET C. ; ARMBRUSTER V. ; GABIOT AC ; GHARBI T. ; BRIDE J. ; HUMBERT P. ; ... Declaration of interest: This study was supported by the Fondation Coloplast pour la Qualite de la Vie of France. ... Faire une nouvelle recherche Make a new search.
It is well known that most odorants stimulate the trigeminal system but the time course of the br... more It is well known that most odorants stimulate the trigeminal system but the time course of the brain regions activated by these chemical stimulations remains poorly documented, especially regarding the trigeminal system. This functional magnetic resonance imaging (fMRI) study compares brain activations resulting from the contrast between two odorant conditions (one bimodal odor and one relatively pure olfactory stimulant) according
Although the cerebral networks involved in sensory perception are of general interest in neurosci... more Although the cerebral networks involved in sensory perception are of general interest in neuroscience, registration of the effects of olfactory stimulation, especially in a magnetic resonance imaging (MRI) environment, presents particular problems and constraints. This article presents details of a reliable and portable system for olfactory stimulation that is modular in design and based on microcontroller technology. It has the following characteristics: (1) It is under software control; (2) the presentation of olfactory stimulation can be synchronized with respiration; (3) it can be manually controlled; and (4) it is fully compatible with an MRI environment. The principle underlying this system is to direct an odor to the subject's nostrils by switching airflow to different odor diffusers. The characteristics of this system were established using (1) ultraviolet (UV) spectroscopy, to measure its response time, and (2) gas chromatography, to measure the repeatability of odor presentation in terms of gas concentration. A response time of 200 ± 25 ms was obtained for the system, and the standard deviations of the gas concentration delivered during stimulation ranged from 1.5% to 22%, depending on the odor, the airflow, and the dilution of the odor used. Since it is portable, controlled by software, and reliable, on the basis of the results we obtained, this system will lend itself to a wide range of applications in olfactory neuroscience.
ABSTRACT The parametric formulation of the combined boundary conditions method (CBCM) with spatia... more ABSTRACT The parametric formulation of the combined boundary conditions method (CBCM) with spatial adaptive resolution is extended to multilayered structures of strip gratings. Furthermore, it is shown that it is not necessary to solve the eigenvalue problem in all the layers of these structures; leading to drastic reduction of the computational load.
Skin topography and microvasculature undergo characteristic changes with age. Although several no... more Skin topography and microvasculature undergo characteristic changes with age. Although several non-invasive bioengineering methods are currently available to measure them quantitatively, few publications have referred to their relationship with age in different anatomical sites. This study was carried out to observe the age-related changes of the skin topography and skin microcirculation. The microrelief was assessed with special processing software from scanning by interference fringe profilometry of silicone replicas performed on two sites (volar forearm and back of hand) on 50 female volunteers (aged 20-74 years who consisted of ten probands in each decade). The superficial vascular network of both sites was assessed by videocapillaroscopy, and the subpapillary vascular plexus was studied with laser Doppler flowmetry. Skin color, which is affected by blood flow, was observed by colorimeter. The skin roughness and the mean height between peak and valley increased with age. There were statistically significant differences between the evaluated sites. This study also shows that the capillary loops in the dermal papillae decrease but the subpapillary plexus increase with age. The interference fringe profilometry associated with videocapillaroscopy may be useful and accurate to measure the efficacy of medical or cosmetic products to delay skin aging.
We describe an interferometric heterodyne vibrometer that uses a laser diode with a triangular mo... more We describe an interferometric heterodyne vibrometer that uses a laser diode with a triangular modulation frequency. This optical sensor is used to probe a vibrating polished surface. As an illustration of the sensor performance, the control of nonuniform velocity of a linear motor is achieved. The technique can be used over a large bandwidth between a few hertz and several tens of kilohertz. Generalization of the technique to the sensing of frequency vibrations is also demonstrated theoretically.
Feedback phenomenon in laser diodes is applied to low-frequency vibration measurement in the micr... more Feedback phenomenon in laser diodes is applied to low-frequency vibration measurement in the micrometer range on poorly reflecting targets. The laser diode is frequency modulated. Its beam is focused on the vibrating target, and backscattered light is fed back into the laser. The optical laser diode power variation is processed to measure the frequency and amplitude of low-frequency vibrations of amplitude, ϳ100 nm to 10 m. Experimental results for sinusoidal and triangular vibrations are compared with theory.
We introduced recently phase measurements usually performed in interferometry to the domain of im... more We introduced recently phase measurements usually performed in interferometry to the domain of image processing and intelligent vision ͓IEEE Trans. Instrum. Meas. 49, 867 ͑2000͔͒. Our purpose is to sense with a high accuracy the position, orientation, and displacement of two-dimensional ͑2D͒ surfaces observed by a static vision system. We report on significant improvements of the method. Experimental measurements reveal a peak-valley noise of approximately 10 Ϫ2 CCD pixel, corresponding approximately to a 10 Ϫ3 period of the phase reference pattern. Then the observation of 10 m scaled features enables an accuracy of a few nm in the position sensing of the phase reference pattern for the extended 2D measurement range.
We present a robust algorithm of phase unwrapping that was designed for use on phase images with ... more We present a robust algorithm of phase unwrapping that was designed for use on phase images with high noise content. We proceed with the algorithm by first identifying regions with continuous phase values placed between fringe boundaries in an image and then phase shifting the regions with respect to one another by multiples of 2 to unwrap the phase. Image pixels are segmented between interfringe and fringe boundary areas by use of a local histogram of a wrapped phase. The algorithm has been used successfully to unwrap phase images generated in a three-dimensional shape measurement for noninvasive quantification of human skin structure in dermatology, cosmetology, and plastic surgery.
Axial resolution of a chromatic dispersion confocal microscopy. [Proceedings of SPIE 5622, 766 (2... more Axial resolution of a chromatic dispersion confocal microscopy. [Proceedings of SPIE 5622, 766 (2004)]. Johnson Garzon Reyes, J. Meneses, Arturo Plata, Gilbert M. Tribillon, Tijani Gharbi. Abstract. An analysis of the axial resolution ...
In radiobiology, low doses of high-LET radiation correspond to a few particle traversals through ... more In radiobiology, low doses of high-LET radiation correspond to a few particle traversals through the cell population. Therefore, for studies on cell monolayers irradiated with a low dose of-particles, it is extremely useful if the number and position of particle traversals can be determined. In this study we describe a new method, based on UV-curing, to obtain a 10 m thick CR-39 grafted onto a 2.5 m thick PolyEthylene Terephtalate (PET). This thin double polymeric layer, used as a dish base, has a regular and reproducible detector thickness which can be traversed by 3.5 MeV-particles, with a sufficient residual energy to traverse mammalian cells attached to the base. The recording properties of a PET-CR-39 dish, together with a demonstration of its use for radiobiological experiments, are presented. This new tool allows the precise determination of single-track impact parameters at a sub-cellular level.
In Near-Field Optical microscopy, the resolution is directly related to the experimental conditio... more In Near-Field Optical microscopy, the resolution is directly related to the experimental conditions of illumination and separation between tip and sample. In general, there is no well-defined linear transfer function of the measurement system. The local resolution can be described by the signal-to-noise ratio, by the separation of individual objects or in terms of data shape sharpness. In this paper we use wavelet analysis to determine local resolution. We deduce the resolution from the characteristics of the wavelet and with the help of the local energy in the decomposition.
The near-field optical images are known to be inhomogeneous due on the one hand to the piezo deca... more The near-field optical images are known to be inhomogeneous due on the one hand to the piezo decay and on the other hand to the scanning process. Both can induce a variation of the tip-sample distance between the beginning and the end of the scanning in constant height mode and steps between consecutive scanning lines. Moreover, the tip often rubs on the sample and induces artifactual, local high variations of the recorded signal. Therefore, a local characterization of spectral information in the signal could be useful in defining the separation power of the setup. This paper proposes a convenient method to evaluate this local spectral information in terms of local characterization of spatial frequency power in the recorded signal. The location of the spatial frequencies is achieved by using the discrete wavelet transform method.
A new fiberized fluorescence imaging probe is presented. This device can potentially be used for ... more A new fiberized fluorescence imaging probe is presented. This device can potentially be used for a wide range of biological or medical applications. By exploiting the chromatic aberrations of gradient index lenses, the excitation blue or near-UV excitation light is focused on the sample surface, while the red fluorescence signal is efficiently launched back to collecting fibers. The excitation fiber is single mode at the working wavelength so that a resolution of 5 m is obtained over a scanning area of several square millimeters. Experimental fluorescence images are presented. They concern either selffabricated fluorescent microsamples or views of leaves that constitute an example of biological tissues analysis. The probe can also be adapted for spectroscopic investigations.
Journal of Engineering Mechanics-asce - J ENG MECH-ASCE, 1997
The precision, accuracy, coordination, and dexterity in hand strength are seen as adequate aspect... more The precision, accuracy, coordination, and dexterity in hand strength are seen as adequate aspects to determine an individual's status and progress. In an attempt to explore those aspects, a cylindrical bar design-based measurement system has been developed. Individual isometric exertions are measured, given visual reference and feedback of the exertion levels using the computer screen display. When processed, the resulted data bring out some parameters in terms of which the aforementioned dynamic aspects can be described. This paper describes this assessment, as well as the hardware, the computer software that constitutes the system, and the calibration method. The test, protocols, and future experiences are stated. The present evaluation could serve as a baseline for diagnosis and assessments in different fields such as surgical procedures evaluation or basketball players hand assessment.
The current work describes the elaboration of a simple, sensitive and reliable -casein modified ... more The current work describes the elaboration of a simple, sensitive and reliable -casein modified glass beads, for the detection and quantification of its specific antibody anti -casein. This is an elementary receptor without electronic part, developed by grafting glass bead surface with the antigenic -casein via 3-aminopropyltriethoxy silane and then glutaraldehyde as cross-linker. The whole is realized by a classical process, called in two steps and in mild conditions where chemical protocol is optimized for -casein use. The detection and quantification of the specific reaction antibody-receptor is carried out by the technique of the second antibody labeled with horse radish peroxidase (HRP). Our receptor can detect the -casein antibody present in the serum at dilutions up to a factor 10 7 in strong ionic strength medium. The same antibody of the same serum and in the same conditions can be detected by ELISA test at dilutions up to a factor 10 5. The whole test, after our receptor realization, takes about 5 h.
... et de Mécanique de Précision, Université Ferhat Abbas [Applied Optics Laboratory, Institute o... more ... et de Mécanique de Précision, Université Ferhat Abbas [Applied Optics Laboratory, Institute of Optics and Precision Mechanics, Ferhat Abbas University ... Optics (Brooklyn, NY: Polytechnic Press) pp 33347 [15] Sumida M and Takemoto K 1984 Lens coupling of laser diodes to ...
... e Laboratoire d'Optique PM Duffieux, University of Franche-Comté, 16 Route d... more ... e Laboratoire d'Optique PM Duffieux, University of Franche-Comté, 16 Route de Gray, 25030 Besançon, France. Received 2 November 2006; revised 8 March 2007; accepted 20 April 2007. ... Thus, poly-l-lysine, fibronectin or laminin are the most commonly used substrates. ...
Optics InfoBase is the Optical Society's online library for flagship journals, partnered and... more Optics InfoBase is the Optical Society's online library for flagship journals, partnered and copublished journals, and recent proceedings from OSA conferences.
... Auteur(s) / Author(s). VIENNET C. ; ARMBRUSTER V. ; GABIOT AC ; GHARBI T. ; BRIDE J. ; HUMBER... more ... Auteur(s) / Author(s). VIENNET C. ; ARMBRUSTER V. ; GABIOT AC ; GHARBI T. ; BRIDE J. ; HUMBERT P. ; ... Declaration of interest: This study was supported by the Fondation Coloplast pour la Qualite de la Vie of France. ... Faire une nouvelle recherche Make a new search.
It is well known that most odorants stimulate the trigeminal system but the time course of the br... more It is well known that most odorants stimulate the trigeminal system but the time course of the brain regions activated by these chemical stimulations remains poorly documented, especially regarding the trigeminal system. This functional magnetic resonance imaging (fMRI) study compares brain activations resulting from the contrast between two odorant conditions (one bimodal odor and one relatively pure olfactory stimulant) according
Although the cerebral networks involved in sensory perception are of general interest in neurosci... more Although the cerebral networks involved in sensory perception are of general interest in neuroscience, registration of the effects of olfactory stimulation, especially in a magnetic resonance imaging (MRI) environment, presents particular problems and constraints. This article presents details of a reliable and portable system for olfactory stimulation that is modular in design and based on microcontroller technology. It has the following characteristics: (1) It is under software control; (2) the presentation of olfactory stimulation can be synchronized with respiration; (3) it can be manually controlled; and (4) it is fully compatible with an MRI environment. The principle underlying this system is to direct an odor to the subject's nostrils by switching airflow to different odor diffusers. The characteristics of this system were established using (1) ultraviolet (UV) spectroscopy, to measure its response time, and (2) gas chromatography, to measure the repeatability of odor presentation in terms of gas concentration. A response time of 200 ± 25 ms was obtained for the system, and the standard deviations of the gas concentration delivered during stimulation ranged from 1.5% to 22%, depending on the odor, the airflow, and the dilution of the odor used. Since it is portable, controlled by software, and reliable, on the basis of the results we obtained, this system will lend itself to a wide range of applications in olfactory neuroscience.
ABSTRACT The parametric formulation of the combined boundary conditions method (CBCM) with spatia... more ABSTRACT The parametric formulation of the combined boundary conditions method (CBCM) with spatial adaptive resolution is extended to multilayered structures of strip gratings. Furthermore, it is shown that it is not necessary to solve the eigenvalue problem in all the layers of these structures; leading to drastic reduction of the computational load.
Skin topography and microvasculature undergo characteristic changes with age. Although several no... more Skin topography and microvasculature undergo characteristic changes with age. Although several non-invasive bioengineering methods are currently available to measure them quantitatively, few publications have referred to their relationship with age in different anatomical sites. This study was carried out to observe the age-related changes of the skin topography and skin microcirculation. The microrelief was assessed with special processing software from scanning by interference fringe profilometry of silicone replicas performed on two sites (volar forearm and back of hand) on 50 female volunteers (aged 20-74 years who consisted of ten probands in each decade). The superficial vascular network of both sites was assessed by videocapillaroscopy, and the subpapillary vascular plexus was studied with laser Doppler flowmetry. Skin color, which is affected by blood flow, was observed by colorimeter. The skin roughness and the mean height between peak and valley increased with age. There were statistically significant differences between the evaluated sites. This study also shows that the capillary loops in the dermal papillae decrease but the subpapillary plexus increase with age. The interference fringe profilometry associated with videocapillaroscopy may be useful and accurate to measure the efficacy of medical or cosmetic products to delay skin aging.
We describe an interferometric heterodyne vibrometer that uses a laser diode with a triangular mo... more We describe an interferometric heterodyne vibrometer that uses a laser diode with a triangular modulation frequency. This optical sensor is used to probe a vibrating polished surface. As an illustration of the sensor performance, the control of nonuniform velocity of a linear motor is achieved. The technique can be used over a large bandwidth between a few hertz and several tens of kilohertz. Generalization of the technique to the sensing of frequency vibrations is also demonstrated theoretically.
Feedback phenomenon in laser diodes is applied to low-frequency vibration measurement in the micr... more Feedback phenomenon in laser diodes is applied to low-frequency vibration measurement in the micrometer range on poorly reflecting targets. The laser diode is frequency modulated. Its beam is focused on the vibrating target, and backscattered light is fed back into the laser. The optical laser diode power variation is processed to measure the frequency and amplitude of low-frequency vibrations of amplitude, ϳ100 nm to 10 m. Experimental results for sinusoidal and triangular vibrations are compared with theory.
We introduced recently phase measurements usually performed in interferometry to the domain of im... more We introduced recently phase measurements usually performed in interferometry to the domain of image processing and intelligent vision ͓IEEE Trans. Instrum. Meas. 49, 867 ͑2000͔͒. Our purpose is to sense with a high accuracy the position, orientation, and displacement of two-dimensional ͑2D͒ surfaces observed by a static vision system. We report on significant improvements of the method. Experimental measurements reveal a peak-valley noise of approximately 10 Ϫ2 CCD pixel, corresponding approximately to a 10 Ϫ3 period of the phase reference pattern. Then the observation of 10 m scaled features enables an accuracy of a few nm in the position sensing of the phase reference pattern for the extended 2D measurement range.
We present a robust algorithm of phase unwrapping that was designed for use on phase images with ... more We present a robust algorithm of phase unwrapping that was designed for use on phase images with high noise content. We proceed with the algorithm by first identifying regions with continuous phase values placed between fringe boundaries in an image and then phase shifting the regions with respect to one another by multiples of 2 to unwrap the phase. Image pixels are segmented between interfringe and fringe boundary areas by use of a local histogram of a wrapped phase. The algorithm has been used successfully to unwrap phase images generated in a three-dimensional shape measurement for noninvasive quantification of human skin structure in dermatology, cosmetology, and plastic surgery.
Axial resolution of a chromatic dispersion confocal microscopy. [Proceedings of SPIE 5622, 766 (2... more Axial resolution of a chromatic dispersion confocal microscopy. [Proceedings of SPIE 5622, 766 (2004)]. Johnson Garzon Reyes, J. Meneses, Arturo Plata, Gilbert M. Tribillon, Tijani Gharbi. Abstract. An analysis of the axial resolution ...
In radiobiology, low doses of high-LET radiation correspond to a few particle traversals through ... more In radiobiology, low doses of high-LET radiation correspond to a few particle traversals through the cell population. Therefore, for studies on cell monolayers irradiated with a low dose of-particles, it is extremely useful if the number and position of particle traversals can be determined. In this study we describe a new method, based on UV-curing, to obtain a 10 m thick CR-39 grafted onto a 2.5 m thick PolyEthylene Terephtalate (PET). This thin double polymeric layer, used as a dish base, has a regular and reproducible detector thickness which can be traversed by 3.5 MeV-particles, with a sufficient residual energy to traverse mammalian cells attached to the base. The recording properties of a PET-CR-39 dish, together with a demonstration of its use for radiobiological experiments, are presented. This new tool allows the precise determination of single-track impact parameters at a sub-cellular level.
In Near-Field Optical microscopy, the resolution is directly related to the experimental conditio... more In Near-Field Optical microscopy, the resolution is directly related to the experimental conditions of illumination and separation between tip and sample. In general, there is no well-defined linear transfer function of the measurement system. The local resolution can be described by the signal-to-noise ratio, by the separation of individual objects or in terms of data shape sharpness. In this paper we use wavelet analysis to determine local resolution. We deduce the resolution from the characteristics of the wavelet and with the help of the local energy in the decomposition.
The near-field optical images are known to be inhomogeneous due on the one hand to the piezo deca... more The near-field optical images are known to be inhomogeneous due on the one hand to the piezo decay and on the other hand to the scanning process. Both can induce a variation of the tip-sample distance between the beginning and the end of the scanning in constant height mode and steps between consecutive scanning lines. Moreover, the tip often rubs on the sample and induces artifactual, local high variations of the recorded signal. Therefore, a local characterization of spectral information in the signal could be useful in defining the separation power of the setup. This paper proposes a convenient method to evaluate this local spectral information in terms of local characterization of spatial frequency power in the recorded signal. The location of the spatial frequencies is achieved by using the discrete wavelet transform method.
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