Papers by Gerardo Castañeda
… Cafeeira, 3. Londrina …, 1999
... Autor : SAUCEDO C., G.; ROMANO M., JM; GUTIERREZ S., G.; DELGADO V., F.; RAMIREZ R., G.; PERR... more ... Autor : SAUCEDO C., G.; ROMANO M., JM; GUTIERREZ S., G.; DELGADO V., F.; RAMIREZ R., G.; PERRAUD G., I ... En los meses de recolección de la cereza del café, la atención está orientada hacia el beneficiadero del café y el manejo de subproductos puede ser problemático ...
International audienceCuando el café no es procesado adecuadamente existe un problema potencial d... more International audienceCuando el café no es procesado adecuadamente existe un problema potencial de contaminación degranos de café por ocratoxina A (OTA) producida por el hongo Aspergillus carbonarius. Esto se puedesolucionar mediante el control biológico del hongo empleando bacterias ácido lácticas (BAL). Estasbacterias tienen requerimientos nutricionales exigentes, por lo que en este trabajo se diseña un medio decultivo de bajo costo para el crecimiento de BAL. El diseño se llevó a cabo mediante la sustitución de dosfuentes de N del medio MRS (Man Rogosa Sharpe) por una fuente de alterna, el extracto de germinadode malta (EGM), Asimismo, mediante un diseño Plackett-Burman (PB) se variaron las concentracionesde otros nutrientes, así como el pH y la temperatura. Las variables de respuesta fueron la productividad yel incremento de productividad de la biomasa de BAL. El EGM resultó ser una fuente alternativa de Neficaz además se encontró que los factores que tienen un mayor efecto sobr...
Revista Mexicana de Ingeniería Química, 2020
Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)
Hydroxycinnamic acids (HAs) have a potential application in the food and pharmaceutical industry ... more Hydroxycinnamic acids (HAs) have a potential application in the food and pharmaceutical industry because they are rich in phenolics. Feruloyl esterases release phenolic compounds from plant cell walls. Coffee pulp is rich in HAs linked to polysaccharides. A solvent extraction of free HAs was performed with aqueous methanol (80 %). A response surface methodology was applied to optimise the extraction of these compounds from coffee pulp, and the best results were obtained at 56°C for 34 min. Alkaline and acid hydrolyses were performed to evaluate the content of linked HAs. Treated (extracted) coffee pulp was used to produce feruloyl esterases in solid-state fermentation by Aspergillus tamarii V12307, previously selected by a hydrolysis plate assay. Different dilutions of a culture medium were added to the coffee pulp, and the diluted medium with half the nutrients allowed for higher CO 2 production. A specific growth rate (m CO 2) of 0.25 h-1 and a lag phase (t lag) of 14.3 h were observed under the selected conditions. Finally, enzymatic activities were 14.0 and 10.8 nkat per g of dried matter when methyl and ethyl ferulate were used as substrates, respectively. Productivities (9.3 and 7.2 nkat per g of dried matter per day, respectively) were higher when compared to other studies carried out in solid-state fermentation. Utilisation of coffee pulp for enzyme production improves the added value of this abundant by-product of the coffee industry.
Ferulic, caffeic, p-coumaric and chlorogenic acids are classified as hydroxycinnamic acids, prese... more Ferulic, caffeic, p-coumaric and chlorogenic acids are classified as hydroxycinnamic acids, presenting anticarcinogenic, anti-inflammatory and antioxidant properties. In this work, enzymatic extraction has been studied in order to extract high value-added products like hydroxycinnamic acids from coffee pulp. A commercial pectinase and enzyme extract produced by Rhizomucor pusillus strain 23aIV in solid-state fermentation using olive oil or coffee pulp (CP) as an inducer of the feruloyl esterase activity were evaluated separately and mixed. The total content (covalently linked and free) of ferulic, caffeic, p-coumaric and chlorogenic acids was 5276 mg per kg of coffee pulp. Distribution was as follows (in %): chlorogenic acid 58.7, caffeic acid 37.6, ferulic acid 2.1 and p-coumaric acid 1.5. Most of the hydroxycinnamic acids were covalently bound to the cell wall (in %): p-coumaric acid 97.2, caffeic acid 94.4, chlorogenic acid 76.9 and ferulic acid 73.4. The content of covalently linked hydroxycinnamic acid was used to calculate the enzyme extraction yield. The maximum carbon dioxide rate for the solid-state fermentation using olive oil as an inducer was higher and it was reached in a short cultivation time. Nevertheless, the feruloyl esterase (FAE) activity (units per mg of protein) obtained in the fermentation using CP as an inducer was 31.8 % higher in comparison with that obtained in the fermentation using olive oil as the inducer. To our knowledge, this is the first report indicating the composition of both esterified and free ferulic, caffeic, p-coumaric and chlorogenic acids in coffee pulp. The highest yield of extraction of hydroxycinnamic acids was obtained by mixing the produced enzyme extract using coffee pulp as an inducer and a commercial pectinase. Extraction yields were as follows (in %): chlorogenic acid 54.4, ferulic acid 19.8, p-coumaric acid 7.2 and caffeic acid 2.3. An important increase in the added value of coffee pulp was mainly due to the extraction of chlorogenic acid.
Effect of initial glucose concentration and inoculation level of lactic acid bacteria in shrimp w... more Effect of initial glucose concentration and inoculation level of lactic acid bacteria in shrimp waste ensilation. Enzyme Microb
International audienceLas bacterias ácido lácticas (BAL), son ampliamente utilizadas en la indust... more International audienceLas bacterias ácido lácticas (BAL), son ampliamente utilizadas en la industria alimentos; poseen lacapacidad de inhibir el crecimiento y desarrollo de patógenos. El medio de cultivo comúnmente utilizadopara el cultivo de BAL es el MRS (Man Rogosa Sharpe), una de las fuentes de nitrógeno: el extracto delevadura está asociado a la producción de compuestos con capacidad antifúngica. En este trabajo seevaluaron 3 concentraciones (2.5, 5 y7 g/l) de extracto de levadura sobre la capacidad antifúngica de doscepas de bacterias ácido lácticas (BAL 7 y BAL 031) para inhibir el crecimiento de Aspergilluscarbonarius DO162 y DO089 provenientes de Costa de Marfil. El efecto se determinó midiendo elcrecimiento radial del hongo en cajas Petri utilizando la técnica de cultivos envenenados empleando agarCzapeck-Extracto de Levadura (CYA).Se determinó que el extracto de levadura no tiene un efecto significativo sobre la inhibición delcrecimiento del hongo, por lo que podría ser r...
Journal of Chemical Technology & Biotechnology, 2021
Applied Biochemistry and Biotechnology, 2021
Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented... more Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF). Fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with various temperatures, sucrose concentrations, and feed flow rates. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70 °C for at least 70 h of continuous operation. In a 20-mL packed bed reactor, the highest hydrolysis rate (12.3 g/g db h) was obtained at 40 °C with 2 M sucrose. Continuous hydrolysis in 20-mL and 200-mL reactors at 60 °C led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification, and immobilisation stages offers an economical alternative for developing accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.
Journal of Physics Communications, 2021
This study presents results of the preparation, absorption and emission characterizations of chlo... more This study presents results of the preparation, absorption and emission characterizations of chlorophyll samples extracted from green microalgae, based on the importance of this pigment and its optical properties. In this work, we characterized extracts containing chlorophyll from green microalgae of the genus Chlorella (C. vulgaris and C. protothecoides) via absorption and emission spectroscopies. The present results were compared with those of chlorophyll extracted from grass, previously reported. Microalgal chlorophyll samples were covalently bonded to the pore surface of organo-modified silica xerogels for a major spectroscopic analysis. Our findings indicate that the composition of green microalgal extracts corresponds mainly to chlorophyll a with a minimal amount of β-carotene. UV-VIS, IR absorption and UV-VIS emission spectra of the samples displayed the typical bands of chlorophyll a, and some other bands ascribed to β-carotene. Both the obtained absorption and emission spectra identify the algae chlorophyll bands and are unique. An effect of a radiative energy transfer mechanism between the silica matrix and the microalgal chlorophyll was observed as reabsorption bands in the emission spectra, and this effect was more evident in the C. vulgaris sample with respect to C. protothecoides.
Advances in Solid State Fermentation, 1997
A new culture technique was developed for studying the physiology of Pleurotus mycelium in solid ... more A new culture technique was developed for studying the physiology of Pleurotus mycelium in solid state fermentation (SSF) system. This method consists of utilization sugar cane bagasse, a by-product of sugar industry, as solid support for absorbing a liquid culture medium. Compared with classical techniques, it offers the advantage of better control of liquid medium composition as well as culture conditions. Fermenters were coupled with on-line gas analysis by using a gas chromatograph equipped with thermal conductivity detector. Solid state culture were aerated at a very low flow air rate (0.04 ml/g moist solids). Concentrations of CO2 and O2 in the exhaust air from fermenters were continuously analysed. By means of this technique, culture conditions were optimized respect to the ratio urea/ammonium sulphate, the particle size of bagasse and inoculation rate. Under best conditions, nearly 90% of substrate was consumed in 6 days. This culture technique offers, excellent practicability for the development of mycelium inocula for commercial exploitation of Pleurotus spp.
Solid-state fermentation of coffee pulp with Aspergillus tamarii V12307 was carried out in labora... more Solid-state fermentation of coffee pulp with Aspergillus tamarii V12307 was carried out in laboratory scale reactors (bottle and column) to evaluate the effect of four different mixing frequencies (2.0, 2.7, 4.0 and 8.0 day-1) on fungal growth, indirectly determined by carbon dioxide formation and the production of spores and pectin methylesterase. Coffee pulp was used as the sole source of nutrients. An increase in the fraction of bonded particles was observed in the bottle reactors after 12 h of cultivation when no mixing was applied. The use of any mixing frequency reduced the fraction of bonded particles. However, there was no significant difference in pectin methylesterase production between the mixing frequencies at the end of the fermentation. Similarly, there were no significant differences in CO 2 production, oxygen uptake or sporulation, demonstrating that the mycelium was not damaged by intermittent mixing. This strategy of mixing could be used in large scale reactors in order to reduce heat and mass limitations.
Environmental Biotechnology, 1995
Coffee Biotechnology and Quality, 2000
... Association Scientifique Internationale du Cafe (ASIC), 15eme Colloque, Montpellier, France D... more ... Association Scientifique Internationale du Cafe (ASIC), 15eme Colloque, Montpellier, France De Rozo M P., Velez J., Garcia L A.(1985) Efecto de ... D.(1985), Planta productora de hongos comestibles sobre pulpa de cafe\ Ciencia y Desarollo, 65: 41-48 Hakil M., Denis S., Viniegra ...
Current Developments in Biotechnology and Bioengineering, 2018
Abstract Solid-state fermentation (SSF) is characterized by its complexity and heterogeneity. Use... more Abstract Solid-state fermentation (SSF) is characterized by its complexity and heterogeneity. Use of agro-industrial residues as substrate avoids accurate measurement of biomass and substrates during the SSF process. Exhaust gas analysis has been used to indirectly estimate the biomass growth; however, these devices don't allow simultaneous measurements from different experimental units or bioreactors and they don't allow accurate simultaneous measurement of CO2, O2, and air-flow rate. A respirometry system based on CO2, O2, and air-flow measurement detectors fed by a system of 15 solenoid valves controlled by a programmable logic controller and a data logger was developed and evaluated during the growth of Rhizomucor pusillus in SSF. The respirometry system was compared to a classical chromatographic respirometer. Accurate measurements of CO2, O2, and air-flow rate allow precise and instantaneous estimation of some kinetic parameters associated with the respiration of the culture such as the specific CO2 production rate and the respiratory quotient. The respirometry system herein described allows online measurements of the microbial process allowing control procedures to be set in SSF processes.
Handbook of Research on Food Science and Technology, 2019
Micologia Neotropical Aplicada, 1991
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Papers by Gerardo Castañeda