Papers by Georgiana Badea
International Journal of Molecular Sciences
The main strategy of this study was to combine the traditional perspective of using medicinal ext... more The main strategy of this study was to combine the traditional perspective of using medicinal extracts with polymeric scaffolds manufactured by an engineering approach to fabricate a potential dressing product with antimicrobial properties. Thus, chitosan-based membranes containing S. officinalis and H. perforatum extracts were developed and their suitability as novel dressing materials was investigated. The morphology of the chitosan-based films was assessed by scanning electron microscopy (SEM) and the chemical structure characterization was performed via Fourier transform infrared spectroscopy (FTIR). The addition of the plant extracts increased the sorption capacity of the studied fluids, mainly at the membrane with S. officinalis extract. The membranes with 4% chitosan embedded with both plant extracts maintained their integrity after being immersed for 14 days in incubation media, especially in PBS. The antibacterial activities were determined by the modified Kirby–Bauer disk ...
Revista de Chimie, 2017
Indole-3-acetic acid is a growth phytohormone considered the most important representative of aux... more Indole-3-acetic acid is a growth phytohormone considered the most important representative of auxin class. This paper presents the assessment of some kinetic parameters in the process of transport of indole-3-acetic acid taking into consideration the kinetic model of consecutive irreversible first order reactions. It was pursued the influence upon the process of parameters such as: feed phase concentration, stripping phase concentration in the presence of two type carriers: tributyl phosphate (TBP) and trioctylphosphine oxide (TOPO). Depending on these transport parameters were calculated kinetics parameters such as: pseudo-first-order apparent membrane entrance and exit rate constants, the maximum flux at the entrance and exit out of the membrane. The highest values of the transport flux is obtained in the presence of carrier trioctylphosphine oxide (TOPO) at the concentration in the feed phase of 10-4 mol/L indole-3-acetic acid and a concentration of 10--2mol/L NaOH in the strippi...
Revista de Chimie, 2019
The paper presents a study of the phenomena that take place at membrane system interfaces in the ... more The paper presents a study of the phenomena that take place at membrane system interfaces in the process of indole-3-acetic acid (IAA) transport. The results were obtained in a bulk liquid membrane system using trioctylamine, tributylphosphate, trioctilphosphine oxide as carriers in chloroform. The main equilibriums that take place at the interface feed phase% membrane phase were identified and the diffusion coefficient of the indole-3-acetic acid complex (DLS) and the extraction constant (Kex) were assessed. The influence of the chemical potential gradient on these parameters was considered.
Romanian Biotechnological Letters
Central European Journal of Chemistry
A fast and selective method based on high-performance liquid chromatography with diode array and ... more A fast and selective method based on high-performance liquid chromatography with diode array and atmospheric pressure chemical ionization-mass spectrometry detection (HPLC-DAD-APCI-MS) was developed and optimized to assess specific food samples content in carotenoids. Two compounds were chosen as significant carotenoids with respect to nutritional value, beta-carotene, and lycopene. The optimum conditions suitable for samples analysis were: a mobile phase of methanol:water (95:5) (A) and acetonitrile:methylene chloride (90:10) (B), with a gradient elution: 0-4 min 0% B, 4-7 min 20% B, 7-10 min 50% B, 0-10 min flow 0.1 mL/min, 10-30 min 90% B, 10-30 min flow 0.2 mL/min. Analytes were separated within 30 min on a C18 column and a diode array detection (450 nm for beta-carotene and 470 nm for lycopene).The linear dependence between peak area and concentration ranged from 5x10-7 molL-1 to 5x10-5 molL-1 (r=0.9998, n=9) for beta-carotene and respectively 7.5x10-7 molL-1 to 7.5x10-5 molL-1...
Infrared Spectroscopy - Materials Science, Engineering and Technology, 2012
Consequently, we consider that the versatility of FTIR techniques (especially variable angle refl... more Consequently, we consider that the versatility of FTIR techniques (especially variable angle reflectance-FTIR, VAR-FTIR and attenuated transmittance-FTIR, ATR-FTIR) in solving these critical issues are presented and, moreover, supported with original experimental data. 2. Applicability of Fourier Transform Infrared technique in the assessment of archeological samples. Case study-Amber Archaeology is a science dealing mainly with the reconstruction of past events by the mean of a cautious credentials and artefacts collection. Historical archaeologists usually encounter www.intechopen.com Infrared Spectroscopy-Materials Science, Engineering and Technology 354 on a list of so-called potential trace evidence, different items which require certain identification; the mentioned list may be formed by objects from various materials: paint, pottery, glass, bricks, liquid residues (oils, wines, and perfumes), fabrics, soil, metals etc. In order to rationalize on the evidences origin, source, transformation and historical age, the archaeologists need a deep analytical study, study that often has to be performed on irreplaceable pieces. Consequently, to accomplish these goals multiple analytical techniques, able to work as non-destructive techniques are used, such as: Fourier Transform Infrared Spectrometry (FTIR), scanning electron microscopy (SEM), polarized light microscopy (PLM) or X-photon spectrometry (XPS).
Talanta, 2014
Head space (HS) solid-phase micro-extraction (SPME) combined with gas chromatography-mass spectro... more Head space (HS) solid-phase micro-extraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to analyze the volatile fraction of ambers of different geological origin. In particular, Romanian (romanite) and Baltic (succinite) amber samples were studied. Both types of amber have nearly similar bulk chemical compositions and could probably reflect only some differences of paleobiological and/or diagenetic origin. The present study shows that amber head space fingerprint, obtained by SPME/GC-MS, can provide a simple and quasi non-destructive method capable of romanite/succinite differentiation. Among the numerous compounds present in the head space, a number of few informative variables could be selected that were able to differentiate the ambers as demonstrated by Principal Component and Cluster Analysis.
Journal of Archaeological Science, 2010
Amber beads from the Cioclovina hoard (Hunedoara County, Romania), dating from the transitional p... more Amber beads from the Cioclovina hoard (Hunedoara County, Romania), dating from the transitional period between the Late Bronze Age and the Iron Age, were analysed by Fourier transform infrared spectroscopy – variable angle reflectance (FTIR-VAR) and Fourier transform Raman spectroscopy (FT-Raman). The aim was to determine if the raw material came from the shores of the Baltic Sea, on the
Journal of Archaeological Science, 2012
In the present study Fourier transform infrared spectroscopy in the reflectance mode (FTIR-reflec... more In the present study Fourier transform infrared spectroscopy in the reflectance mode (FTIR-reflectance) e was performed on well preserved archaeological amber, originating from three Romanian archaeological sites of Roman and Byzantine cultures, which did not allow sampling material. Resulting spectra were subjected to multivariate data analysis (MDA) using Principal Components Analysis (PCA). In the light of the FTIR-reflectance bands of archaeological samples from the region 1275 to 1155 cm À1 and 900e600 cm À1 we could conclude that the samples from first two locations, Roş ia Montan a and Nuf aru, are made from amber of Baltic origin. In the case of the third location, Isaccea, we take into account the possibility of a mixture of raw materials, both Baltic and Romanian amber. These assertions are supported by statistical analysis and the historical context. Besides the FTIR spectra comparison or other analytical data, it is very important to correlate any information about the material (colour, conservation status, historical context, connections with other artefacts) and the statistical analysis in order to favour safe conclusions.
Lasparaginase was immobilized by entrapment in hydrogelmagnetic nanoparticles obtained from magne... more Lasparaginase was immobilized by entrapment in hydrogelmagnetic nanoparticles obtained from magnetic nanoparticles and successive layers of chitosan and hyaluronic acid. The obtained nanostructures, with dimensions suitable for penetration cell/tissues, especially tumor tissues, were evaluated for swelling behavior, degradation behavior and the release of Lasparaginase in neutral media and slight acid media (pH 4.5). The experiments showed a good swelling behavior for all the tested samples, but a high stability of nanostructures. Lasparaginase has an initial burst from hydrogel, at two hours, and other two releases at 24 and between 50 and 72 hours.
Chemical Papers, 2014
Increased need for non-destructive investigation methods in archaeology has become a major issue ... more Increased need for non-destructive investigation methods in archaeology has become a major issue since sampling is in most cases restricted in view of the importance or uniqueness of the objects. For this reason, preliminary investigation using non-destructive techniques was performed on five samples of amber beads obtained from different excavation and archaeological sites. The use of FTIR and micro-Raman analysis revealed the presence of carboxyl, peroxide, hydroxyl, and complex ester functional groups as well as single and double bonds in the structure of the studied resin varieties. Further analysis of the amber samples from both archaeological and geological types by XPS, XRF, and SEM showed the presence of sulfur and a wide range of trace elements on the surface of the analysed samples. Our results proved that the combination of structural-molecular and surface elemental techniques for amber characterisation provides a very useful and simple methodology for the description of ...
Open Chemistry, 2009
The study aims to establish several definite criteria which will differentiate Romanian amber and... more The study aims to establish several definite criteria which will differentiate Romanian amber and Baltic amber to certify the local or Baltic origin of the materials found in archaeological sites on the Romanian territory, by using light microscopy and performing analytical methods, such as Fourier transform infrared spectroscopy-variable angle reflectance and liquid chromatography with mass spectrometry detection. Experiments especially by Fourier transformed infrared spectroscopy, were applied to a wide range of samples with controlled origin. The methods were optimised and resulted in premises to apply the techniques to analysis of the archaeological material.
Open Chemistry, 2012
This work focuses on using analytical methods, such as Fourier transform Infrared spectroscopy (F... more This work focuses on using analytical methods, such as Fourier transform Infrared spectroscopy (FTIR) and high performance liquid chromatography (HPLC) with mass spectrometry (MS) detection to assess archaeological and geological amber. The main goal of this study is to apply the previously developed and optimized analytical methods in verifying criteria to ascribe and characterize the origin of materials found in archaeological sites. The proposed LC-MS method was successfully applied for the quantification of succinic acid content both in geological and archaeological samples of amber and offers excellent linearity between 0.1 and 5µg mL−1. The developed FTIR method provided some criteria which is able to differentiate between Baltic and Romanian amber (Romanite) that furthermore validates on archaeological amber artefacts.
Archaeometry, 2013
Ten geological samples (five from each source—Romanian amber and Baltic amber) in duplicate for f... more Ten geological samples (five from each source—Romanian amber and Baltic amber) in duplicate for five different media (air, water, saline, acid and basic) were subjected to thermal alteration at 80 ± 2°C for 12 weeks, in the dark. The samples were analysed before and after the accelerated alteration experiment by Fourier transform infrared spectroscopy, both in transmittance and reflectance mode, and the acquired spectra were statistically processed by multivariate data analysis, mainly using principal component analysis. The results of the experiment are useful in giving a better picture of how to discriminate between archaeological amber artefacts.
Analytical Methods, 2013
ABSTRACT A simple, fast and sensitive high-performance liquid chromatography/ electrospray ioniza... more ABSTRACT A simple, fast and sensitive high-performance liquid chromatography/ electrospray ionization-mass spectrometry method is described and validated in terms of linearity, accuracy/recovery and reproducibility, as well as limit of detection for the determination of ascorbic acid from grape (Vitis vinifera L.) samples from Murfatlar vineyard. Good linearity (correlation factor > 0.9970) was achieved in the concentration range 0.5-15 μg mL-1 for AA in acetic acid 0.1% solution. The obtained RSD values (below 5%) indicated excellent repeatability of the proposed method. The limit of detection was 0.32 μg mL-1 while the recovery ranged between 96.86 and 102.41%. The advantages of the method are: small amounts of sample and solvents, short analysis time and minimum steps for sample preparation.
Revista de Chimie -Bucharest- Original Edition-
A simple, fast and sensitive capillary electrophoresis method was developed and partially validat... more A simple, fast and sensitive capillary electrophoresis method was developed and partially validated for the determination of several water-soluble vitamins from food samples. The separation of five vitamins from group B and ascorbic acid was carried out by using capillary electrophoresis with a fused-silica capillary and UV detection (214 nm). The optimum conditions were: 20mM NaH 2 PO 4 and 15mM SDS background electrolyte, pH=9 and voltage 25kV. The method exhibited good results concerning precision (RSD %< 5), linear calibration curves (R 2 >0.99) and selectivity in the concentration range studied. The advantages of the method are: small amounts of sample and reagents, short analysis time and minimum steps. The results were confirmed and are comparable to those obtained by high performance liquid chromatography coupled with mass spectrometry.
Carboxylic Acid - Key Role in Life Sciences, 2018
Revista de Chimie, 2017
A fast separation method for simultaneous determination of eleven organic acids was validated and... more A fast separation method for simultaneous determination of eleven organic acids was validated and applied to different commercial food items to evaluate their organic acids content. The present method gives detection limits between 0.04 and 4.65 mg mL-1, recovery values in real samples between 78.2 and 97.3% and relative standard deviation values for precision lower than 5%. All validation data were in acceptable range and prove the method�s fit for purpose. The advantages of the method are the short runtime analysis (15 min), no preparation step for the samples before the injection combined with good sensitivity which recommends it for routine control analysis in food industries. Moreover, this methodology has high potential in drinks industry but can by further extended to other types of food items.
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Papers by Georgiana Badea