Papers by Gabriela Garcia
The American Journal of Tropical Medicine and Hygiene, 2009
The present work deals with the identification of a patient with two liver abscesses containing t... more The present work deals with the identification of a patient with two liver abscesses containing two different strains of Entamoeba histolytica , as defined by chitinase gene plymorphisms.
The American Journal of Tropical Medicine and Hygiene, 2002
In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is... more In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).
Molecular and Biochemical Parasitology, 2005
Molecular and biochemical parasitology, 2005
The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an impo... more The intra-species polymorphism of E. histolytica and E. dispar species in endemic area is an important tool for geographic distribution and spread mechanism studies. Since E. dispar and E. histolytica shears ecological niche, cell cycle, and transmission mechanism for human host, we studied the intra-specie variation and distribution of E. dispar strains obtained from cyst passers, in two neighbor rural communities in Morelos Mexico. We analyzed the polymorphic region of the quitinase protein gene in isolates of E. dispar. In 45 isolates from one community we identified 12 different CHI patterns while in 15 isolates from the other community we identified 5 different patterns. However both communities share 4 patterns. This finding suggests the presence of strains with different geographic mobility.
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Papers by Gabriela Garcia