Papers by Gloria Invernici
Diabetologia, 2006
Aims/hypothesis: Benfotiamine, a vitamin B1 analogue, reportedly prevents diabetic microangiopath... more Aims/hypothesis: Benfotiamine, a vitamin B1 analogue, reportedly prevents diabetic microangiopathy. The aim of this study was to evaluate whether benfotiamine is of benefit in reparative neovascularisation using a type I diabetes model of hindlimb ischaemia. We also investigated the involvement of protein kinase B (PKB)/Akt in the therapeutic effects of benfotiamine. Methods: Streptozotocin-induced diabetic mice, given oral benfotiamine or vehicle, were subjected to unilateral limb ischaemia. Reparative neovascularisation was analysed by histology. The expression of Nos3 and Casp3 was evaluated by real-time PCR, and the activation state of PKB/Akt was assessed by western blot analysis and immunohistochemistry. The functional importance of PKB/Akt in benfotiamine-induced effects was investigated using a dominant-negative construct. Results: Diabetic muscles showed reduced transketolase activity, which was corrected by benfotiamine. Importantly, benfotiamine prevented ischaemia-induced toe necrosis, improved hindlimb perfusion and oxygenation, and restored endothelium-dependent vasodilation. Histological studies revealed the improvement of reparative neovascularisation and the inhibition of endothelial and skeletal muscle cell apoptosis. In addition, benfotiamine prevented the vascular accumulation of advanced glycation end products and the induction of pro-apoptotic caspase-3, while restoring proper expression of Nos3 and Akt in ischaemic muscles. The benefits of benfotiamine were nullified by dominant-negative PKB/Akt. In vitro, benfotiamine stimulated the proliferation of human EPCs, while inhibiting apoptosis induced by high glucose. In diabetic mice, the number of circulating EPCs was reduced, with the deficit being corrected by benfotiamine. Conclusions/ interpretation: We have demonstrated, for the first time, that benfotiamine aids the post-ischaemic healing of diabetic animals via PKB/Akt-mediated potentiation of angiogenesis and inhibition of apoptosis. In addition, benfotiamine combats the diabetes-induced deficit in endothelial progenitor cells.
Experimental Cell Research, Jul 1, 2010
Introduction: Myocardial Infarction (MI) represents one of principal causes of human death. Cell-... more Introduction: Myocardial Infarction (MI) represents one of principal causes of human death. Cell-based therapy could be a valid new approach for MI. Different kind of cells, including embryonic stem cells as well as adult/progenitor stem cells, have been proposed as candidates for therapeutic purposes. However, many aspects, ranging from ethical questions to functional efficacy, still remain to be clarified. Among adult/progenitor stem cells, Adipose-derived stromal cells (ADSC) seem to have some advantages, mainly because of their easy tissue accessibility and iv tro an adequate rate of growth. Aim of the study: To investigate the capacity of transplanted ADSCs, through functional, haemodynamic and histopathological assessment, to improve myocardial infarction and regeneration of experimental heart ischemia induced by permanent IVA-ligation in pigs. Methods: ADSCs isolated from human adipose tissue (omentum fat) were cultured, expanded, and phenotipically characterized. Furthermore, in vitro proangiogenic, anti-inflammatory and anti-apoptotic properties were analyzed. 50x10 6 cells/pig were transplanted by intramyocardial injection in acute infarcted hearts (treated-group, n=12 cell-injected pigs). Two months after MI induction echocardiographyc and haemodinamic follow-up was performed. In addition, hystopathological examination was conducted.
Supplementary Figure 1 from Transforming Growth Factor-β1 and CD105 Promote the Migration of Hepa... more Supplementary Figure 1 from Transforming Growth Factor-β1 and CD105 Promote the Migration of Hepatocellular Carcinoma–Derived Endothelium
Surgical neurology international, 2018
Proof of the efficacy and safety of a xenogeneic mesenchymal stem cell (MSCs) transplant for spin... more Proof of the efficacy and safety of a xenogeneic mesenchymal stem cell (MSCs) transplant for spinal cord injury (SCI) may theoretically widen the spectrum of possible grafts for neuroregeneration. Twenty rats were submitted to complete spinal cord transection. Ovine bone marrow MSCs, retrovirally transfected with red fluorescent protein and not previously induced for neuroglial differentiation, were applied in 10 study rats (MSCG). Fibrin glue was injected in 10 control rats (FGG). All rats were evaluated on a weekly basis and scored using the Basso-Beattie-Bresnahan (BBB) locomotor scale for 10 weeks, when the collected data were statistically analyzed. The spinal cords were then harvested and analyzed with light microscopy, immunohistochemistry, and immunofluorescence. Ovine MSCs culture showed positivity for Nestin. MSCG had a significant and durable recovery of motor functions (<.001). Red fluorescence was found at the injury sites in MSCG. Positivity for Nestin, tubulin βIII...
Decellularized silk fibroin scaffold primed with adipose mesenchymal stro diabetic mice, both Ad-... more Decellularized silk fibroin scaffold primed with adipose mesenchymal stro diabetic mice, both Ad-MSCs-SF and D-Ad-MSCs-SF significantly improved tissue regeneration, reducing the wound area respectively by 40 % and 35%, within three days, completing the process in around 10 days compared to Navone et al. Stem Cell Research & Therapy 2014, 5:7
L'invention concerne un milieu de culture pour l'isolement et la propagation de lignees c... more L'invention concerne un milieu de culture pour l'isolement et la propagation de lignees cellulaires animales de purete elevee issues de structures vasculaires et/ou endotheliales des systemes cardio-circulatoire et/ou mesodermique, comprenant des glucides, des sels inorganiques, des vitamines, des acides amines essentiels pour la synthese proteique, comprenant au moins de la glutamine, des hemoderives ou similaires, pour la contribution d'au moins des metalloproteines, des hormones et des facteurs trophiques, presents dans des proportions variables afin d'assurer un equilibre trophique pour la proliferation cellulaire, et ayant un pH se situant dans la plage entre 6,8 et 7,4.
Circulation, Oct 31, 2007
Circulation, Oct 31, 2006
Diritto Processuale Amministrativo, 2013
SOMMARJO:1. Introduzione. - 2. L'art. 34, comma 3, del Codice del processo amministrativo. - ... more SOMMARJO:1. Introduzione. - 2. L'art. 34, comma 3, del Codice del processo amministrativo. - 3. L'interpretazione giurisprudenziale: due tesi a confronto.
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Papers by Gloria Invernici