Papers by Fereshteh karamali
Journal of Materials Chemistry B, 2017
Functionalized nanofibrous scaffolds with decellularized ECM (DECM) particles can mimic the natur... more Functionalized nanofibrous scaffolds with decellularized ECM (DECM) particles can mimic the natural motifs of cartilage ECMs and enhance chondro-inductivity for tissue engineering applications.
Objective: The aim of this study was to produce a stable CHO cell line expressing tenecteplase.Ma... more Objective: The aim of this study was to produce a stable CHO cell line expressing tenecteplase.Materials and Methods: In the first step, the tenecteplase coding sequence was clonedin a pDB2 vector containing attB recognition sites for the phage φC31 integrase. Then,using lipofection, the CHO cells were co-transfected with constructed recombinant plasmidencoding tenecteplase and attB recognition sites and the integrase coding sequencecontaining pCMV-Int plasmid. As the recombinant plasmid contained the neomycin resistancegene (neo), stable cells were then selected using G418 as an antibiotic. Stabletransformed cells were assessed using genomic PCR and RT-PCR. Finally, the functionalityof tenecteplase was evaluated on the cell culture media.Results: our results indicated that tenecteplase coding sequence was inserted into theCHO cell genome and was successfully expressed. Moreover, tenecteplase activity assessmentindicated the presence of our functional tenecteplase in the cell culture medium.Conclusion: Considering the data obtained from this study, φC31 integrase can be usedfor the production of a stable cell line and it be used to introduce ectopic genes into mammaliancells.
Embryonic stem cells derived from a preimplantation embryo appear to have an unlimited capacity t... more Embryonic stem cells derived from a preimplantation embryo appear to have an unlimited capacity to self-renew in cell culture, and they are also able to differentiate into hundreds of adult cell types. Human embryonic stem cell lines offer a platform technology that has the potential ...
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 2012
Methamphetamine (MA) is the most popular recreational drug. According to potential neurotoxicity ... more Methamphetamine (MA) is the most popular recreational drug. According to potential neurotoxicity of this agent, it can cause deleterious effects on neural differentiation of embryo, if MA is used during the child bearing period. In recent decades, undifferentiated pluripotent embryo-derived stem cell lines, resembling early embryonic stages, have been used to analyze the toxic effects of components in vitro. Thus, this study aims at assessing toxic effects of MA on embryonic stem cell (ESC)-derived neuronal cells during differentiation in a pharmacological model. ESC line Royan was used throughout this study. The effect of MA on neural differentiation was assessed during two periods, group 1: MA (10, 100, 200,500, 750, 1000 μM concentrations) was added during EB formation, group 2: MA (10, 50, 70, 100, 200, 500 μM concentrations) was added after the generation of neural precursors. Then cells were evaluated for neuronal markers by immunocytochemistry and RT-PCR. One way ANOVA follow...
Advances in Materials Science and Engineering
One promising treatment for degenerative retinal diseases such as age-related macular degeneratio... more One promising treatment for degenerative retinal diseases such as age-related macular degeneration (AMD) is the delivery of retinal pigment epithelial (RPE) cells using degradable scaffolds. Tough-aligned scaffolds are promising candidates for some applications of tissue engineering, such as peripheral nerve regeneration. However, aligned scaffolds have not been investigated in retinal tissue engineering so far. Here, a comparison was made between aligned and random scaffolds fabricated from polycaprolactone (PCL) and human amniotic membrane powder (HAMP) as a scaffold for RPE cells. The effects of alignment on mechanical properties, porosity, hydrophilicity, degradation of the scaffolds, and the cellular interaction of RPE cells were investigated. The results revealed that the aligned scaffold has a lower average fiber diameter, porosity, hydrophilicity, and Young’s modulus and also a higher maximum strain in failure compared with the random scaffold. However, the proliferation of ...
Page 1. 1 بوﺮﻜﻴﻣ ﺪﺷرا سﺎﻨﺷرﺎﻛ ﻲﺳﺎﻨﺷ ،ﻲﻜﺷﺰﭘ اد هﺪﻜﺸﻧ ي ناﺮﻳا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲ... more Page 1. 1 بوﺮﻜﻴﻣ ﺪﺷرا سﺎﻨﺷرﺎﻛ ﻲﺳﺎﻨﺷ ،ﻲﻜﺷﺰﭘ اد هﺪﻜﺸﻧ ي ناﺮﻳا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ . 2 هﺪﻜﺸﻨﻧاد ،يژﻮﻟﻮﻴﺑوﺮﻜﻴﻣ هوﺮﮔ ،رﺎﻴﺸﻧاد ي ناﺮﻳا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ،ﻲﻜﺷﺰﭘ . 3 اﺮﻫﺰﻟا ﻲﻜﺷﺰﭘ ﺰﻛﺮﻣ ،ﻲﻫﺎﮕﺸﻳﺎﻣزآ مﻮﻠﻋ سﺎﻨﺷرﺎﻛ ) س ( ناﺮﻳا ،نﺎﻬﻔﺻا ،نﺎﻬﻔﺻا ﻲﻜﺷﺰﭘ مﻮﻠﻋ هﺎﮕﺸﻧاد ، . ...
Molecular and Cellular Neuroscience, 2003
Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been repo... more Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been reported to exhibit neurotrophic activity in addition to being expressed in different parts of the nervous system. At present, the effects of HGF on neural stem cells (NSCs) are not known. In this study, we first report the promoting effect of HGF on the proliferation of neurospheres and neuronal differentiation of NSCs. Medium containing only HGF was capable of inducing neurosphere formation. Addition of HGF to medium containing fibroblast growth factor 2 or epidermal growth factor increased both the size and number of newly formed neurospheres. More neurons were also obtained when HGF was added in differentiation medium. In contrast, neurosphere numbers were reduced after repeated subculture by mechanical dissociation, suggesting that HGF-formed neurospheres comprised predominantly progenitor cells committed to neuronal or glial lines. Together, these results suggest that HGF promotes proliferation of neurospheres and neuronal differentiation of NSCs derived from mouse embyos.
Journal of Biological Chemistry
Cell journal, Mar 1, 2022
Objective Degeneration of the photoreceptors due to retinal disorders can affect vision, and even... more Objective Degeneration of the photoreceptors due to retinal disorders can affect vision, and even lead to blindness. Recently therapeutic progress in retinal degeneration, using human embryonic stem cells (hESCs), has been facing technical challenges, demanding the development of simple and standardized protocols. In addition to the designing of the protocols, characterization of the obtained cells is highly required for confirming the reliability of the applied methods for future medical applications. Previously, we showed that human stem cells from apical papilla (SCAP) have stromal cell-derived inducing activity (SDIA). Materials and Methods In this experimental study, we developed an efficient retinal differentiation protocol, based on the co-culture of confluent hESCs and SCAP in the absence of exogenous molecules, such as activators or inhibitors of molecular signaling pathways. This experimental procedure resulted in the generation of self-forming neural retina (NR)-like structures containing retinal progenitor cells (RPCs) within 4 weeks. Results We have focused on the characterization of the derived RPCs, as a crucial step towards further verification of the efficiency of our previously suggested protocol. The differentiated cells expressed eye-field markers, PAX6, RAX, LHX2, and SIX3, and also generated neurospheres by a floating culture system for one week. Conclusion We have reported that the treatment of hESC-derived RPCs by the Notch pathway-inhibitor induced the generation of photoreceptor precursor cells (PPCs). The presented method demonstrates the fact that a co-culture of hESCs and SCAP without exogenous molecules provides an efficient approach to produce RPCs for the treatment of retinal disease, and act as an in vitro model for the development of human retina.
Journal of Bioscience and Bioengineering, 2022
Tissue-specific extracellular matrix (ECM) plays a critical role in cell survival and homeostasis... more Tissue-specific extracellular matrix (ECM) plays a critical role in cell survival and homeostasis, which are particularly essential for directing differentiation of different complex tissues such as retina. However, ECM maintenance should be considered to design an effective therapeutic strategy for retina regeneration. To achieve this, cell sheet engineering has emerged as a growing approach to closely reconstruct basal membrane of cells through a scaffold-free manner. Several irreversible sight-threatening diseases are characterized by the dysfunction and lose of retinal pigment epithelium (RPE), leading to vision loss and eventually total blindness in patients. According to impressive developments in achievement of RPE from human embryonic stem cells (hESCs), we obtained RPE cells without any extrinsic factors in a co-culture system, and cultured them on a temporary alginate hydrogel substrate. Subsequently, Arg-Gly-Asp (RGD) peptide was superficially immobilized on the upper layer of hydrogel to improve cell attachment before harvesting sheet layer. RPE cell sheet layer was released by treating pre-seeded hydrogels with sodium citrate as a calcium chelating agent and characterized in both in vitro and in vivo models. RPE sheets formed tight junction and expressed high levels of retina structural markers such as ZO-1, Bestrophin and Collagen type IV. One week after in vivo transplantation of RPE sheet, cells survived in the subretinal space, indicating that our harvesting method is non-invasive. To sum up, we introduced a unique scaffold-free method for RPE cell sheet engineering, which can find potential use for future therapeutic purposes.
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Papers by Fereshteh karamali