Papers by Fabiana Resende Rodrigues
Open Journal of Obstetrics and Gynecology, 2018
Vaginal tumors, whether benign or malignant, are rare. They include fibroepithelial polyps (FEPs)... more Vaginal tumors, whether benign or malignant, are rare. They include fibroepithelial polyps (FEPs), which are benign lesions originating in mesenchymal cells, comprised of a core of connective tissue covered by squamous epithelium. They are usually small and asymptomatic. When symptomatic or very large, they may cause bleeding, genital discomfort or the presence of a bulge in the vagina. In the last case, they may be mistaken for a genital prolapse. Although their physiopathology is still not clearly understood, the presence of hormonal receptors and the occurrence of FEPs during the use of hormone therapy or pregnancy suggest that changes in the stroma of these lesions may be induced by hormones. We report on the case of a patient who presented with a vaginal bulge and was referred to the urogynecology outpatient ward with a diagnosis of genital prolapse, which had actually a large fibroepithelial polyp on the posterior vaginal wall.
BMC cancer, May 2, 2018
NKX2.5 is a transcription factor transiently expressed during thyroid organogenesis. Recently, se... more NKX2.5 is a transcription factor transiently expressed during thyroid organogenesis. Recently, several works have pointed out the oncogenic role of NKX2.5 in a variety of tumors. We therefore hypothesized that NKX2.5 could also play a role in thyroid cancer. The validation of NKX2.5 expression was assessed by immunohistochemistry analysis in a Brazilian case series of 10 papillary thyroid carcinoma (PTC) patients. Then, the long-term prognostic value of NKX2.5 and its correlation with clinicopathologic features of 51 PTC patients was evaluated in a cohort with 10-years follow-up (1990-1999). Besides, the effect of NKX2.5 overexpression on thyroid differentiation markers and function was also investigated in a non-tumor thyroid cell line (PCCL3). NKX2.5 was shown to be expressed in most PTC samples (8/10, case series; 27/51, cohort). Patients who had tumors expressing NKX2.5 showed lower rates of persistence/recurrence (p = 0.013). Overexpression of NKX2.5 in PCCL3 cells led to: 1) d...
Jornal Brasileiro de Doenças Sexualmente Transmissíveis, 2011
The exact role that viruses play in tumorigenesis is unclear, but it seems they are responsible f... more The exact role that viruses play in tumorigenesis is unclear, but it seems they are responsible for causing one of a series of steps required for the development of cancer. The first step for infering whether a particular cancer is caused by this virus is showing the virus in tissue degradation. Molecular techniques, in comparison with the evaluation of antibodies in immunohistochemistry, are the most effective techiniques to establish the presence of the virus. Human papillomavirus (HPV) and Epstein-Barr virus (EBV) have been found in breast carcinomas worldwide. The high-risk human papillomavirus may be an important risk factor for breast carcinogenesis and metastasis. The role of human papillomavirus in breast carcinogenesis is still unclear and may ultimately be determined by monitoring the incidence of future breast cancer among women vaccinated for human papillomavirus types as high-risk.
Journal of Cancer Science & Therapy, 2012
Introduction: Breast cancer is the second most common cancer worldwide. Adequate evaluation of th... more Introduction: Breast cancer is the second most common cancer worldwide. Adequate evaluation of the specimen is essential for accurate diagnosis and prognosis. Objective: To present a standardized protocol for the gross and microscopic examination of radical mastectomy specimens, despite the presence or absence of homolateral axillary dissection and irrespective of the type of the malignancy to provide the necessary information to guide potential adjuvant therapy and prognostic information. Materials and methods: A standardized protocol was created for the examination of surgical specimens from radical mastectomies. Results: The group of pathologists responsible for routine examination agreed that use of the standardized protocol reduced the time spent on macroscopic and microscopic examination of the radical mastectomy specimens and increased the accuracy of recording the information needed for diagnosis. Conclusion: We believe that simplification of the protocol presented here for guidance of histological examination of surgical mastectomy specimens is easily applied, rapid, reliable and safe, and will contribute to the routine diagnostic practice.
Journal of Cancer Science & Therapy, 2014
Introduction: The isolation of lymph nodes less than 4.0 mm in diameter from the fatty tissue of ... more Introduction: The isolation of lymph nodes less than 4.0 mm in diameter from the fatty tissue of surgical breast cancer specimens through the traditional method of tactile perception is difficult, time consuming and failed the identification of smaller lymph nodes. The reported numbers of lymph nodes detected through this technique vary widely in the literature demonstrating its inappropriateness. The use of fat clearance techniques however may contribute to the isolation of additional nodes, allowing for appropriate pathological staging of the disease. Objective: To evaluate the impact of the use of fat clearance machinery and solutions in the dissection and identification of lymph nodes from axillary dissections of patients with breast cancer. Materials and methods: Fat clearance techniques (cooker, electric spiral, modified Koren clearing solution and formaldehyde-acetic alcohol solution) were applied to 100 breast cancer axillary dissections from males and females with no age restriction over a period of two years (2009-2011). Results: Through the use of fat clearance methods, additional 174 metastases in 564 lymph nodes were found, more than the classical method, from which 449 metastases in 1426 lymph nodes were identified. There was no statistical difference between the fat clearance methods; however the cooker method demonstrated greater efficiency than the modified Koren clearing solution method. Conclusion: The fat clearance methods described here have various advantages over the classical method. These methods are viable, rapid, practical, inexpensive and do not alter the quality of histological analysis or immunohistochemical reactions. The findings show that examination of a minimum of 20 isolated lymph nodes is necessary for optimal identification and diagnosis of lymph node metastases. The main outcome from this study were the immediate benefits generated by adopting new technical procedures resulting in a change in pathological staging classification in 14% of the patients.
Journal of Cancer Science and Clinical Therapeutics, 2020
The inducible inflammatory enzyme cyclooxygenase-2 (COX-2) favors carcinogenesis, but its express... more The inducible inflammatory enzyme cyclooxygenase-2 (COX-2) favors carcinogenesis, but its expression in breast tumors presents great variability, with controversial prognostic impact. Here, we characterize COX-2 protein levels in breast tumors by immunohistochemistry according to gene polymorphisms, and evaluate if tumor COX-2 protein levels or mRNA are associated with survival outcomes.
Breast Cancer: Targets and Therapy
This study aimed to compare the clinical behavior, clinicopathological and sociodemographic chara... more This study aimed to compare the clinical behavior, clinicopathological and sociodemographic characteristics of patients with early-stage triple-negative breast cancer (TNBC) who belong to the HER2-low and HER2-zero subgroups. Patients and Methods: This study involved a thorough search in the internal database of a single Brazilian institution to identify women with TNBC who underwent neoadjuvant chemotherapy (NACT) followed by curative surgery within the period from January 2010 to December 2014. HER2 analysis through immunohistochemistry (IHC) and, if required, amplification by in situ hybridization, was conducted using core biopsy samples. The study assesses outcomes of residual cancer burden (RCB), event-free survival (EFS), and overall survival (OS). Results: A total of 170 cases were analyzed, with a mean age of 51.4 years (standard deviation, SD 11.2). The HER2 status was categorized as IHC 0, 1+, or 2+ in 80 (47.1%), 73 (42.9%), and 17 (10%) patients, respectively. No significant differences were observed in the prevalence of clinical pathological characteristics among the subgroups. The absence of significant results for clinicopathological and demographic features hindered the multivariate analysis of HER2 subgroups. Similarly, no significant differences were found in the RCB, EFS, and OS outcomes between HER2 subgroups. Conclusion: The findings of this study suggest that, in early-stage TNBC, the clinical behavior and survival outcomes of the HER2low subgroup may not differ significantly from those of the HER2-zero subgroup.
BMC Cancer
Objective To examine the prevalence and prognostic role of tumor microenvironment (TME) markers i... more Objective To examine the prevalence and prognostic role of tumor microenvironment (TME) markers in uterine carcinosarcoma (UCS) through immunohistochemical characterization. Methods The internal database of our institution was queried out for women with UCS who underwent surgery and thereafter postoperative chemotherapy with carboplatin and paclitaxel between January 2012 and December 2017. Tissue microarrays containing surgical samples of UCS from 57 women were assessed by immunohistochemistry for CD3, CD4, CD8, FOXP3, PD-1, PD-L1, and PD-L2. Results The mean age was 65.3 years (range, 49 to 79 years). For the epithelial component (E), CD3_E and CD4_E were highly expressed in 38 (66.7%) and in 40 (70.1%) patients, respectively, and were significantly associated with more advanced stages (p = 0.038 and p = 0.025, respectively). CD8_E was highly expressed in 42 (73.7%) patients, FOXP3_E 16 (28.1%), PD-1_E 35 (61.4%), PD-L1_E 27 (47.4%) and PD-L2_E 39 (68.4%). For the sarcomatous comp...
Frontiers in Oncology, 2021
ObjectiveThis study aimed to examine the prevalence and prognostic role of tumor microenvironment... more ObjectiveThis study aimed to examine the prevalence and prognostic role of tumor microenvironment (TME) in triple-negative breast cancer (TNBC) after neoadjuvant chemotherapy (NACT) through immunohistochemical characterization.MethodsThe internal database of the Brazilian National Cancer Institute for women diagnosed with TNBC who underwent NACT and thereafter curative surgery between January 2010 and December 2014 was queried out. Core biopsy specimens and tissue microarrays containing surgical samples of TNBC from 171 and 134 women, respectively, were assessed by immunohistochemistry for CD3, CD4, CD8, CD14, CD56, CD68, CD117, FOXP3, PD-1, PD-L1, and PD-L2. Immune cell profiles were analyzed and correlated with response and survival.ResultsMean age was 50.5 years, and most cases were clinical stage III [143 cases (83.6%)]. According to the multivariate analysis, only Ki67 and clinical stage significantly influenced the pattern of response to systemic treatment (p = 0.019 and p = 0...
Breast Cancer: Targets and Therapy, 2021
India-Developing a knowledge base for policymaking on India-EU migration This project is co-finan... more India-Developing a knowledge base for policymaking on India-EU migration This project is co-financed by the European Union and carried out by the EUI in partnership with the Indian Council of Overseas Employment, (ICOE), the Indian Institute of Management Bangalore Association, (IIMB), and Maastricht University (Faculty of Law). The proposed action is aimed at consolidating a constructive dialogue between the EU and India on migration covering all migration-related aspects. The objectives of the proposed action are aimed at: Assembling high-level Indian-EU expertise in major disciplines that deal with migration (demography, economics, law, sociology and politics) with a view to building up migration studies in India. This is an inherently international exercise in which experts will use standardised concepts and instruments that allow for aggregation and comparison. These experts will belong to all major disciplines that deal with migration, ranging from demography to law and from economics to sociology and political science. Providing the Government of India as well as the European Union, its Member States, the academia and civil society, with: 1. Reliable, updated and comparative information on migration 2. In-depth analyses on India-EU highly-skilled and circular migration, but also on low-skilled and irregular migration. Making research serve action by connecting experts with both policy-makers and the wider public through respectively policy-oriented research, training courses, and outreach programmes. These three objectives will be pursued with a view to developing a knowledge base addressed to policy-makers and migration stakeholders in both the EU and India. Results of the above activities are made available for public consultation through the website of the project: http://www.india-eu-migration.eu/
Journal of Clinical Oncology, 2018
Background. Taxanes usually follow anthracyclines in breast cancer neo/adjuvant treatment, likely... more Background. Taxanes usually follow anthracyclines in breast cancer neo/adjuvant treatment, likely because of their later introduction into clinical practice. However, there is no biological rationale that justifies this current standard of care. We compared a taxane followed by an anthracycline-based regimen with the reverse sequence in the neoadjuvant setting. Patients and Methods. In a randomized, open-label, singlecenter phase II trial, women with inoperable, locally advanced, HER2-negative breast cancer were stratified by hormone receptor status and randomized to three cycles of docetaxel (T) followed by three cycles of fluorouracil, doxorubicin, and cyclophosphamide (FAC) versus three cycles of FAC followed by three cycles of docetaxel. Surgery, radiotherapy, and adjuvant hormonal therapy were administered as per local guidelines. The primary endpoint was pathological complete response (pCR), and secondary endpoints included toxicity, event-free survival (EFS), and overall survival (OS). Results. Treatment sequence did not improve pCR, which was 7% with T-FAC and 3% with FACT. However, after a median follow-up of 79 months, the 5-year EFS rate was 75.7% (95% confidence interval [CI], 65.4%-87.7%) with T-FAC and 48.2% (95% CI, 37.0%-62.7%) with FACT (hazard ratio [HR], 0.46; 95% CI, 0.26-0.81; log-rank p = .0054), and the 5-year OS rate was 89.7% (95% CI, 82.2%-97.8%) with T-FAC and 64.7% (95% CI, 53.6%-78.1%) with FACT (HR, 0.41; 95% CI, 0.22-0.78; p = .0052). There were no unexpected toxicities. Conclusion. We showed for the first time an improvement in EFS and OS with taxane-first compared with anthracyclinefirst sequencing chemotherapy in HER2-negative, locally advanced breast cancer. Confirmation of these results may have implications for clinical practice. This trial was registered with Clinicatrials.gov identifier NCT01270373. The Oncologist 2020;9999:• • Implications for Practice: The NeoSAMBA trial showed a benefit for taxane-first sequencing chemotherapy consistent with the systematic review of the literature as well as the larger Neo-tAnGo study. Many recent and current ongoing clinical trials have already followed this treatment strategy. As a taxane-before-anthracycline sequence carries neither an incremental cost nor an increased toxicity, and given the available literature on this issue, reinforced taxane-first regimen can be easily incorporated into daily clinical practice while we wait for confirmation of these findings from larger trials.
International Journal of General Medicine, 2013
We report two rare cases of breast cancer in men treated with modified radical mastectomy. The pa... more We report two rare cases of breast cancer in men treated with modified radical mastectomy. The patients were aged 67 and 45 years at the time of diagnosis. Tumor sizes were 2 cm and 8 cm in the older and younger patient, respectively. The histopathologic diagnosis was infiltrating ductal carcinoma in the first patient and infiltrating papillary carcinoma in the second patient. Immunohistochemical tests demonstrated estrogen and progesterone receptor positivity and HER2/c-erbB-2 negativity in both cases. The rarity of these cases is noted, in view of their uncommon occurrence at an early age in men and their immunophenotypic positivity, similar to breast cancer in women.
BMC Cancer, 2015
Background: Neurofibromatosis 1 is one of the most common genetic diseases in humans, presenting ... more Background: Neurofibromatosis 1 is one of the most common genetic diseases in humans, presenting with multiple neurofibromas and an increased risk of various benign and malignant tumors, including breast cancer. Case presentation: In this paper we report a case of a woman with neurofibromatosis 1 and the challenge associated with detecting an advanced breast cancer because of numerous skin neurofibromas, which were responsible for a substantial delay in cancer diagnosis. Literature concerning the association of neurofibromatosis 1 and breast cancer is reviewed and discussed. Conclusions: Best practice guidelines for breast cancer detection are not sufficient for the screening of neurofibromatosis 1 carriers. A more intensive clinical and imaging approach should be used if the same early detection rate as in non-neurofibromatosis 1 women is to be achieved.
Brazilian Oral Research, 2013
Oral cytopathology is a simple, non-invasive technique that could be used for early detection of ... more Oral cytopathology is a simple, non-invasive technique that could be used for early detection of oral premalignant and malignant lesions, but the effectiveness of this diagnostic approach remains controversial. The aim of this study was to evaluate the sensitivity, specificity, positive and negative predictive values, and accuracy of cytopathology for diagnosing oral squamous cell carcinoma (OSCC) and the diagnostic concordance between cytopathological and histopathological diagnoses. The study enrolled 172 patients at outpatient clinics who presented with oral lesions suspicious of malignancy. All patients underwent oral cytological scrapes followed by an incisional biopsy. Of 148 cases that were histopathologically diagnosed with OSCC, the cytopathological method diagnosed 123 positive cases and resulted in a suspicion of OSCC in 16 patients. Based on these data, the sensitivity was 83.1%, the specificity was 100.0%, the positive predictive value was 100.0%, the negative predictive value was 49.0%, and the accuracy was 85.5%. The diagnostic concordance between histopathological and cytopathological examinations was 83.1% for OSCC and 85.7% for non-neoplastic lesions. The results indicate that cytopathological diagnosis had good concordance with histopathological diagnosis and showed high sensitivity, specificity, positive predictive value, and accuracy. We conclude that the sensitivity of oral cytopathology is sufficient to justify its use as a diagnostic screening test and to confirm the malignant nature of epithelial cells, mainly for the classification of OSCC. Therefore, cytopathology may be a reliable method for referring patients who require diagnosis of suspected oral cancer for starting treatment.
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2011
Introdução: O declínio no número de autópsias em hospitais e instituições universitárias é evento... more Introdução: O declínio no número de autópsias em hospitais e instituições universitárias é evento amplamente reportado, inicialmente em países do primeiro mundo e posteriormente em muitos países em desenvolvimento, como o Brasil. As causas para essa tendência são múltiplas e complexas, incluindo aspectos religiosos, familiares e médicos. Entre estes últimos, salientam-se razões diagnósticas, caracterizadas pelo significativo avanço tecnológico na imagenologia, com a suposição de que todos os diagnósticos agora podem ser feitos em vida, e a crescente ansiedade de processos judiciais contra médicos por má prática. Objetivo: Demonstrar o decréscimo vertiginoso e drástico do número de autópsias em um hospital universitário no Brasil. Material e método: Avaliou-se o número de registros nos livros de autópsias consecutivas realizadas no
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2013
Fisioterapia Brasil, 2016
Introdução: A crioterapia é um procedimento físico que utiliza gelo e/ou água gelada para baixar ... more Introdução: A crioterapia é um procedimento físico que utiliza gelo e/ou água gelada para baixar a temperatura corporal, com fins terapêuticos, utilizada e estudada há tempos com propósito analgésico e antiedematoso. Objetivo: Determinar a percepção de um grupo de pacientes oncológicos atendidos em um ambulatório de mastologia num hospital universitário. Material e métodos: Este estudo caracteriza-se como uma pesquisa descritiva, realizada no Hospital Universitário Antônio Pedro, em Niterói/RJ, com pacientes portadoras de carcinoma infiltrante de mama e que cumpriram o protocolo de crioterapia proposto: teste de tolerância; aplicação diária em mama durante 15 dias antes e 30 dias após a cirurgia; realização de questionários no 15º e 30º pós-cirúrgico. Resultados: Houve predominância de pacientes que já haviam utilizado a crioterapia como recurso terapêutico, em situações de trauma e tempo de aplicação de 10 minutos. Todas relataram percepção de melhora em graus variados e que aceita...
Jornal Brasileiro de Patologia e Medicina Laboratorial, 2010
Primeira submissão em 08/12/09 Última submissão em 27/12/09 Aceito para publicação em 17/06/10 Pu... more Primeira submissão em 08/12/09 Última submissão em 27/12/09 Aceito para publicação em 17/06/10 Publicado em 20/10/10 1. Mestra em Patologia; professora assistente de Patologia da Universidade Federal Fluminense (UFF). 2. Chefe do Serviço de Anatomia Patológica do Hospital Geral de Bonsucesso; residência médica em Patologia. 3. Mestra em Patologia; professora adjunta de Patologia da UFF. 4. Mestra em Patologia; professora assistente de Patologia da UFF. 5. Professora titular de Patologia da UFF; chefe da pós-graduação da UFF. Investigação da LMP1 do EBV e a coinfeçcão do HPV em lesões genitais de pacientes infectados ou não pelo HIV Investigation of the LMP1 EBV and co-infection by HPV in genital lesions of patients infected or not by HIV
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Papers by Fabiana Resende Rodrigues