During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for p... more During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for phytoplasma presence. ‘ Candidatus Phytoplasma mali’ was detected in 17 samples from phloem tissue of apple shoots showing typical apple proliferation symptoms. Molecular characterization of these strains was conducted using PCR-RFLP and sequence analyses of three regions: ribosomal DNA, ribosomal protein and non-ribosomal DNA fragment including nitroreductase and rhodanese-like protein genes. Fragments of 16S rDNA plus 16S-23S spacer region from the ‘ Ca. P. mali’ strains showed two restriction profiles: P-I and P-II when digested with Hpa II enzyme. Moreover, based on results of nitroreductase and rhodanese-like protein genes, these phytoplasmas were grouped into two subtypes: AP-15 and AT-1. The majority of the ‘ Ca . P. mali’ strains was classified to the AP-15 subtype. Two strains were grouped to the AT-1 subtype and another showed both AP-15 and AT-1 profiles. Based on results of an...
Potato spindle tuber viroid (PSTVd) has a quarantine status in the EU whereas Tomato apical stunt... more Potato spindle tuber viroid (PSTVd) has a quarantine status in the EU whereas Tomato apical stunt viroid (TASVd) is listed in the EPPO Alert list. During 2007 to 2012 surveys for the presence of PSTVd in 299 ornamental plants of the family Solanaceae (including Solanum jasminoides, S. rantonnetti, Brugmansia sp. and Petunia sp.) were carried out in Poland. The availability of a Pospiviroid genus-specific primer pair (1), which allows for the detection of the most prevalent viroids in ornamental plants by RT-PCR, has facilitated surveys of ornamental plants for pospiviroids. Fifteen S. rantonnetti and twenty-one S. jasminoides plants were sampled randomly and tested. Samples originated from seven different Polish provinces. Total RNA extraction was performed from plant leaves using Master Pure RNA Purification Kit (Epicentre). The obtained RNAs were further used for RT-PCR amplification using SuperScript One-Step RT-PCR System with PlatinumTaq DNA Polymerase (Invitrogen) kit accordin...
During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for p... more During 2010-2013, samples from 267 apple trees growing in six regions of Poland were tested for phytoplasma presence. ‘ Candidatus Phytoplasma mali’ was detected in 17 samples from phloem tissue of apple shoots showing typical apple proliferation symptoms. Molecular characterization of these strains was conducted using PCR-RFLP and sequence analyses of three regions: ribosomal DNA, ribosomal protein and non-ribosomal DNA fragment including nitroreductase and rhodanese-like protein genes. Fragments of 16S rDNA plus 16S-23S spacer region from the ‘ Ca. P. mali’ strains showed two restriction profiles: P-I and P-II when digested with Hpa II enzyme. Moreover, based on results of nitroreductase and rhodanese-like protein genes, these phytoplasmas were grouped into two subtypes: AP-15 and AT-1. The majority of the ‘ Ca . P. mali’ strains was classified to the AP-15 subtype. Two strains were grouped to the AT-1 subtype and another showed both AP-15 and AT-1 profiles. Based on results of an...
Potato spindle tuber viroid (PSTVd) has a quarantine status in the EU whereas Tomato apical stunt... more Potato spindle tuber viroid (PSTVd) has a quarantine status in the EU whereas Tomato apical stunt viroid (TASVd) is listed in the EPPO Alert list. During 2007 to 2012 surveys for the presence of PSTVd in 299 ornamental plants of the family Solanaceae (including Solanum jasminoides, S. rantonnetti, Brugmansia sp. and Petunia sp.) were carried out in Poland. The availability of a Pospiviroid genus-specific primer pair (1), which allows for the detection of the most prevalent viroids in ornamental plants by RT-PCR, has facilitated surveys of ornamental plants for pospiviroids. Fifteen S. rantonnetti and twenty-one S. jasminoides plants were sampled randomly and tested. Samples originated from seven different Polish provinces. Total RNA extraction was performed from plant leaves using Master Pure RNA Purification Kit (Epicentre). The obtained RNAs were further used for RT-PCR amplification using SuperScript One-Step RT-PCR System with PlatinumTaq DNA Polymerase (Invitrogen) kit accordin...
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