Erika Pineda

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Maritza Urteaga

Escuela Nacional de Antropologia e Historia

Mariano Fernández-Enguita

Universidad Complutense de Madrid

Ricardo Cuberos Mejía

University of Zulia (Universidad del Zulia)

Igor Israel González Aguirre

Universidad de Guadalajara

Luis Moreno

CSIC (Consejo Superior de Investigaciones Científicas-Spanish National Research Council)

Eloísa del Pino

CSIC (Consejo Superior de Investigaciones Científicas-Spanish National Research Council)

Magdalena Bas

Universidad de la República (Uruguay)

J. Eduardo Sierra-Nieto

Universidad de Málaga

Javier Rujas Martínez-Novillo

Universidad Complutense de Madrid

Revista Innovación Educativa, Instituto Politécnico Nacional

Instituto Politécnico Nacional

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Papers by Erika Pineda

706: Outcome Comparison of Inhaled Anesthetics Versus Ecmo in Children with Near-Fatal Asthma

Critical Care Medicine

P90: Surfactant Use in Pediatric Patients with Acute Respiratory Failure Receiving Extracorporeal Membrane Oxygenation: A Single Center Experience

ASAIO Journal

Estudio y análisis del nivel de gestión del servicio en las operaciones de ti en las empresas prestadoras de servicios de telefonía móvil celular de Colombia

Este documento se basa en el estudio y analisis realizado sobre el nivel de gestion del servicio ... more Este documento se basa en el estudio y analisis realizado sobre el nivel de gestion del servicio en las operaciones de TI de las principales empresas prestadoras de servicios moviles de Colombia. Dado que actualmente en Colombia la demanda de telefonia movil ha crecido de manera significativa y algunos operadores no han aumentado su infraestructura, por tal razon, el servicio se ha visto degradado de gran manera, ademas el sistema de gestion de los procesos internos para llegar a prontas resoluciones no es el mas adecuado para posibilitar las necesidades y requerimientos que surgen.

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Estrategias para la restauración forestal en comunidades del municipio de Motozintla, Chiapas, México

Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline

PLOS Pathogens, 2021

Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), ... more Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), is transmitted by the tsetse fly where the procyclic forms of the parasite develop in the proline-rich (1–2 mM) and glucose-depleted digestive tract. Proline is essential for the midgut colonization of the parasite in the insect vector, however other carbon sources could be available and used to feed its central metabolism. Here we show that procyclic trypanosomes can consume and metabolize metabolic intermediates, including those excreted from glucose catabolism (succinate, alanine and pyruvate), with the exception of acetate, which is the ultimate end-product excreted by the parasite. Among the tested metabolites, tricarboxylic acid (TCA) cycle intermediates (succinate, malate and α-ketoglutarate) stimulated growth of the parasite in the presence of 2 mM proline. The pathways used for their metabolism were mapped by proton-NMR metabolic profiling and phenotypic analyses of thirteen RNA...

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Fly stage trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in near physiological conditions

Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), ... more Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), is transmitted by the tsetse fly, where the procyclic forms of the parasite develop in the proline-rich (1-2 mM) and glucose-depleted digestive tract. Proline is essential for the midgut colonization of the parasite in the insect vector, however other carbon sources could be available and used to feed its central metabolism. Here we show that procyclic trypanosomes can consume and metabolize metabolic intermediates, including those excreted from glucose catabolism (succinate, alanine and pyruvate), with the exception of acetate, which is the ultimate end-product excreted by the parasite. Among the tested metabolites, tricarboxylic acid (TCA) cycle intermediates (succinate, malate and α-ketoglutarate) stimulated growth of the parasite in the presence of 2 mM proline. The pathways used for their metabolism were mapped by proton-NMR metabolic profiling and phenotypic analyses of a dozen RNA...

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Entamoeba histolytica under Oxidative Stress: What Countermeasure Mechanisms Are in Place?

Cells, 2017

Entamoeba histolytica is the causative agent of human amoebiasis; it affects 50 million people wo... more Entamoeba histolytica is the causative agent of human amoebiasis; it affects 50 million people worldwide and causes approximately 100,000 deaths per year. Entamoeba histolytica is an anaerobic parasite that is primarily found in the colon; however, for unknown reasons, it can become invasive, breaching the gut barrier and migrating toward the liver causing amoebic liver abscesses. During the invasive process, it must maintain intracellular hypoxia within the oxygenated human tissues and cellular homeostasis during the host immune defense attack when it is confronted with nitric oxide and reactive oxygen species. But how? This review will address the described and potential mechanisms available to counter the oxidative stress generated during invasion and the possible role that E. histolytica's continuous endoplasmic reticulum (Eh-ER) plays during these events.

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Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica

The FEBS Journal, 2010

The in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties... more The in vitro Entamoeba histolytica pyruvate:ferredoxin oxidoreductase (EhPFOR) kinetic properties and the effect of oxidative stress on glycolytic pathway enzymes and fluxes in live trophozoites were evaluated. EhPFOR showed a strong preference for pyruvate as substrate over other oxoacids. The enzyme was irreversibly inactivated by a long period of saturating O2 exposure (IC50 0.034 mm), whereas short‐term exposure (< 30 min) leading to > 90% inhibition allowed for partial restoration by addition of Fe2+. CoA and acetyl‐CoA prevented, whereas pyruvate exacerbated, inactivation induced by short‐term saturating O2 exposure. Superoxide dismutase was more effective than catalase in preventing the inactivation, indicating that reactive oxygen species (ROS) were involved. Hydrogen peroxide caused inactivation in an Fe2+‐reversible fashion that was not prevented by the coenzymes, suggesting different mechanisms of enzyme inactivation by ROS. Structural analysis on an EhPFOR 3D model...

Pyruvate : ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells

Microbiology, 2011

The Trichomonas vaginalis 120 kDa protein adhesin (AP120) is induced under iron-rich conditions a... more The Trichomonas vaginalis 120 kDa protein adhesin (AP120) is induced under iron-rich conditions and has sequence homology with pyruvate : ferredoxin oxidoreductase A (PFO A), a hydrogenosomal enzyme that is absent in humans. This homology raises the possibility that, like AP120, PFO might be localized to the parasite surface and participate in cytoadherence. Here, the cellular localization and function of PFO that was expressed under various iron concentrations was investigated using a polyclonal antibody generated against the 50 kDa recombinant C-terminal region of PFO A (anti-PFO50). In Western blot assays, this antibody recognized a 120 kDa protein band in total protein extracts, and proteins with affinity to the surface of HeLa cells from parasites grown under iron-rich conditions. In addition to localization that is typical of hydrogenosomal proteins, PFOs that were expressed under iron-rich conditions were found to localize at the surface. This localization was demonstrated us...

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706: Outcome Comparison of Inhaled Anesthetics Versus Ecmo in Children with Near-Fatal Asthma

Critical Care Medicine

P90: Surfactant Use in Pediatric Patients with Acute Respiratory Failure Receiving Extracorporeal Membrane Oxygenation: A Single Center Experience

ASAIO Journal

Estudio y análisis del nivel de gestión del servicio en las operaciones de ti en las empresas prestadoras de servicios de telefonía móvil celular de Colombia

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Estrategias para la restauración forestal en comunidades del municipio de Motozintla, Chiapas, México

Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline

PLOS Pathogens, 2021

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Fly stage trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in near physiological conditions

Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), ... more Trypanosoma brucei, a protist responsible for human African trypanosomiasis (sleeping sickness), is transmitted by the tsetse fly, where the procyclic forms of the parasite develop in the proline-rich (1-2 mM) and glucose-depleted digestive tract. Proline is essential for the midgut colonization of the parasite in the insect vector, however other carbon sources could be available and used to feed its central metabolism. Here we show that procyclic trypanosomes can consume and metabolize metabolic intermediates, including those excreted from glucose catabolism (succinate, alanine and pyruvate), with the exception of acetate, which is the ultimate end-product excreted by the parasite. Among the tested metabolites, tricarboxylic acid (TCA) cycle intermediates (succinate, malate and α-ketoglutarate) stimulated growth of the parasite in the presence of 2 mM proline. The pathways used for their metabolism were mapped by proton-NMR metabolic profiling and phenotypic analyses of a dozen RNA...

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Entamoeba histolytica under Oxidative Stress: What Countermeasure Mechanisms Are in Place?

Cells, 2017

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Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica

The FEBS Journal, 2010

Pyruvate : ferredoxin oxidoreductase (PFO) is a surface-associated cell-binding protein in Trichomonas vaginalis and is involved in trichomonal adherence to host cells

Microbiology, 2011

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